Click here to switch to the map view.

The map label for this gene is pyrB [H]

Identifier: 78048617

GI number: 78048617

Start: 3485984

End: 3486931

Strand: Reverse

Name: pyrB [H]

Synonym: XCV3061

Alternate gene names: 78048617

Gene position: 3486931-3485984 (Counterclockwise)

Preceding gene: 78048618

Following gene: 78048616

Centisome position: 67.34

GC content: 68.04

Gene sequence:

>948_bases
ATGACCACCATGCAACTCGATTCGGACGGACGCCTGCGCCACCTGCTCACCCTGGAAGGACTGCCGCGCACCACGCTGCT
GCAATTGCTCGACCGCGCCGGCCAGATCCGCGATGCGGCGGTGGGGCGCGTGGGCAAGCGCAGCGTGCTGGCGGGCACGG
CGGTGTGCACGCTGTTCTTCGAACCCTCCACGCGCACGCGCAGCTCCTTCCATCTGGCGGCGCAGCGGCTGGGCGCGGAC
GTGCTCAATTTCGATGCATCCACCTCATCCACCCGCAAGGGCGAAACCGCGCGCGACACCTTGAAGAATCTCGAAGCGAT
GGGCGTGCGCGGCTTCGTGGTGCGTCACCCCGACGACGGCGCGGTGGAAGCGCTGGCGACCGCCGCGGGCGAGGGCACCG
CGTTGATCAATGCCGGCGACGGACGCAGCGCGCATCCTACCCAGGGCCTGCTCGACATGCTCACCCTGCGCCAGGCCAAG
GGCACCGATTTTTCCAAGCTCAAGGTCGTTATCGTCGGCGACGTCAAGCACTCGCGTGTGGCGCGCTCGGACCTGCATGC
CCTGCGCACGCTGGGCGCTGGCGAGATCCGCGTCTGCGGCCCGGCCAGCCTGCTGCCGGACGACGGCATACTGGAGGGCT
GCGTGGTCGGCCAGGATTTCGATGCCATGCTCGAAGGCGCCGACGCGTTGATGATGCTGCGCCTGCAGCGCGAGCGCATG
GAAGAAGGCCTGGTGCCCTCGCTGGAGCAGTACCACACCGAATATGGTCTGACCCGTGAGCGTCTGGCGCGTGCTGGCCG
CGACGCCGCGGTGCTGCACCCGGGCCCGATCAACCGCGGCGTTGAAATCACCGATGAAGTGGCCGACGGCGCGCAATCCT
GCGTGCTGCGTCAGGTTGCCAATGGCGTTGCCGTACGCATGGCCGTGCTGGAAACCCTGCTCGGCTAA

Upstream 100 bases:

>100_bases
GACGCCGAAGCGCTCGATGCGATGGCCGCCGCAGTGATCGTCGAACGCTGGTTGGCCGCGCCCGACCAAGCCACCTCTCT
TCCTTGACTCCCGACCTGCG

Downstream 100 bases:

>100_bases
GCGCGGCTGCGCCGTCAGATCGTGAGTCGCTCCAGGAGCGGCGCCCCCACCGCGCAACCAACGCGCGGTGGCCTTTGATC
CATGAGGCGTCGATTGCGAA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MTTMQLDSDGRLRHLLTLEGLPRTTLLQLLDRAGQIRDAAVGRVGKRSVLAGTAVCTLFFEPSTRTRSSFHLAAQRLGAD
VLNFDASTSSTRKGETARDTLKNLEAMGVRGFVVRHPDDGAVEALATAAGEGTALINAGDGRSAHPTQGLLDMLTLRQAK
GTDFSKLKVVIVGDVKHSRVARSDLHALRTLGAGEIRVCGPASLLPDDGILEGCVVGQDFDAMLEGADALMMLRLQRERM
EEGLVPSLEQYHTEYGLTRERLARAGRDAAVLHPGPINRGVEITDEVADGAQSCVLRQVANGVAVRMAVLETLLG

Sequences:

>Translated_315_residues
MTTMQLDSDGRLRHLLTLEGLPRTTLLQLLDRAGQIRDAAVGRVGKRSVLAGTAVCTLFFEPSTRTRSSFHLAAQRLGAD
VLNFDASTSSTRKGETARDTLKNLEAMGVRGFVVRHPDDGAVEALATAAGEGTALINAGDGRSAHPTQGLLDMLTLRQAK
GTDFSKLKVVIVGDVKHSRVARSDLHALRTLGAGEIRVCGPASLLPDDGILEGCVVGQDFDAMLEGADALMMLRLQRERM
EEGLVPSLEQYHTEYGLTRERLARAGRDAAVLHPGPINRGVEITDEVADGAQSCVLRQVANGVAVRMAVLETLLG
>Mature_314_residues
TTMQLDSDGRLRHLLTLEGLPRTTLLQLLDRAGQIRDAAVGRVGKRSVLAGTAVCTLFFEPSTRTRSSFHLAAQRLGADV
LNFDASTSSTRKGETARDTLKNLEAMGVRGFVVRHPDDGAVEALATAAGEGTALINAGDGRSAHPTQGLLDMLTLRQAKG
TDFSKLKVVIVGDVKHSRVARSDLHALRTLGAGEIRVCGPASLLPDDGILEGCVVGQDFDAMLEGADALMMLRLQRERME
EGLVPSLEQYHTEYGLTRERLARAGRDAAVLHPGPINRGVEITDEVADGAQSCVLRQVANGVAVRMAVLETLLG

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=310, Percent_Identity=34.5161290322581, Blast_Score=149, Evalue=2e-36,
Organism=Escherichia coli, GI2367364, Length=312, Percent_Identity=31.7307692307692, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1786469, Length=343, Percent_Identity=25.9475218658892, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790703, Length=335, Percent_Identity=25.6716417910448, Blast_Score=68, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=293, Percent_Identity=33.1058020477816, Blast_Score=111, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6322331, Length=319, Percent_Identity=33.5423197492163, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24642586, Length=313, Percent_Identity=33.5463258785943, Blast_Score=145, Evalue=4e-35,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 33667; Mature: 33536

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTMQLDSDGRLRHLLTLEGLPRTTLLQLLDRAGQIRDAAVGRVGKRSVLAGTAVCTLFF
CCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHEEEEEEEE
EPSTRTRSSFHLAAQRLGADVLNFDASTSSTRKGETARDTLKNLEAMGVRGFVVRHPDDG
CCCCCCHHHHHHHHHHHCCHHEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCH
AVEALATAAGEGTALINAGDGRSAHPTQGLLDMLTLRQAKGTDFSKLKVVIVGDVKHSRV
HHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHHEEEEEEECCHHHHH
ARSDLHALRTLGAGEIRVCGPASLLPDDGILEGCVVGQDFDAMLEGADALMMLRLQRERM
HHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEECCCHHHHHHCHHHHHHHHHHHHHH
EEGLVPSLEQYHTEYGLTRERLARAGRDAAVLHPGPINRGVEITDEVADGAQSCVLRQVA
HCCCCCCHHHHHHHHCCCHHHHHHCCCCCEEECCCCCCCCCEEHHHHHHHHHHHHHHHHH
NGVAVRMAVLETLLG
HHHHHHHHHHHHHHC
>Mature Secondary Structure 
TTMQLDSDGRLRHLLTLEGLPRTTLLQLLDRAGQIRDAAVGRVGKRSVLAGTAVCTLFF
CEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHEEEEEEEE
EPSTRTRSSFHLAAQRLGADVLNFDASTSSTRKGETARDTLKNLEAMGVRGFVVRHPDDG
CCCCCCHHHHHHHHHHHCCHHEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCH
AVEALATAAGEGTALINAGDGRSAHPTQGLLDMLTLRQAKGTDFSKLKVVIVGDVKHSRV
HHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHHEEEEEEECCHHHHH
ARSDLHALRTLGAGEIRVCGPASLLPDDGILEGCVVGQDFDAMLEGADALMMLRLQRERM
HHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEECCCHHHHHHCHHHHHHHHHHHHHH
EEGLVPSLEQYHTEYGLTRERLARAGRDAAVLHPGPINRGVEITDEVADGAQSCVLRQVA
HCCCCCCHHHHHHHHCCCHHHHHHCCCCCEEECCCCCCCCCEEHHHHHHHHHHHHHHHHH
NGVAVRMAVLETLLG
HHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA