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The map label for this gene is dsbA [H]

Identifier: 78047871

GI number: 78047871

Start: 2647616

End: 2648260

Strand: Reverse

Name: dsbA [H]

Synonym: XCV2315

Alternate gene names: 78047871

Gene position: 2648260-2647616 (Counterclockwise)

Preceding gene: 78047872

Following gene: 78047869

Centisome position: 51.14

GC content: 60.31

Gene sequence:

>645_bases
ATGAAACGACGTCATTTTCTGATTGCCGCTGCGATGGTGCCCTTGGCGCCGCTGGCCAATAGCGTTTCCGCCCAAGAGGC
TTTCAAAGCGGGCAAGGATTTCCTGGAGCTGGACAAACCCGTGCCGGTCGATGCGCCGCCTGGCAAGGTGGAGGTCATTG
AGTTCTTCTCGTACAACTGTCCGCACTGCGCTGCCTTCGAGCCGCAGCTCGAAATCTGGACGAGGCAACTTCCCTCCATC
GCATCCTTCCGGCGCGTTCCTGTGCCCTTTGTAGGCAGTGACGTGGAAGCCAAACAGCGCATGTACTACGCACTGCAGGC
TCTGGGCAAAGAGGACGAATTCCGACCCAGGATCTTCCGGGCCATCCATCAAGAGCACCAACGCCTGTTCGGCGACGATG
CCATCTTAGCTTGGGCCGACAAGCAACCTGGACTGGACGGCAAGAAGTTCGCCGAGGCGTATCACTCGTTCTCGACCATG
ACCAGAGCCAAACGCGCAACGCAGACGACCAGTGATTTCAAGGTGGCGGGTGTGCCGGCGCTAGGCATCGCCGGACGTTG
GTACGCGGACGGAGAAACCGCCGGCAGCCCCGAGCGCGCGCTTCAGGTAACCAGTTTCCTGATTCGGGAAGCAAGCCGGG
CGTGA

Upstream 100 bases:

>100_bases
CCTCGTTCCCGATGATGGCGAAAGCTACAGCTTCTGAATGGCCCTTTCACCAGACCGACAGTGGTATCGGAGCAGTCCCG
ACCCTGGAGATACCCCCCGC

Downstream 100 bases:

>100_bases
GGATTGCCCGGTACTGCTCAGGAGACGGGGCCGGTGCTGCCTTGCAGCGCAAGGCCCGCCGCCCGCCATTCGGGCACGCC
CCCCGCCAGTGGCGTGGCCT

Product: putative thiol:disulfide interchange protein

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MKRRHFLIAAAMVPLAPLANSVSAQEAFKAGKDFLELDKPVPVDAPPGKVEVIEFFSYNCPHCAAFEPQLEIWTRQLPSI
ASFRRVPVPFVGSDVEAKQRMYYALQALGKEDEFRPRIFRAIHQEHQRLFGDDAILAWADKQPGLDGKKFAEAYHSFSTM
TRAKRATQTTSDFKVAGVPALGIAGRWYADGETAGSPERALQVTSFLIREASRA

Sequences:

>Translated_214_residues
MKRRHFLIAAAMVPLAPLANSVSAQEAFKAGKDFLELDKPVPVDAPPGKVEVIEFFSYNCPHCAAFEPQLEIWTRQLPSI
ASFRRVPVPFVGSDVEAKQRMYYALQALGKEDEFRPRIFRAIHQEHQRLFGDDAILAWADKQPGLDGKKFAEAYHSFSTM
TRAKRATQTTSDFKVAGVPALGIAGRWYADGETAGSPERALQVTSFLIREASRA
>Mature_214_residues
MKRRHFLIAAAMVPLAPLANSVSAQEAFKAGKDFLELDKPVPVDAPPGKVEVIEFFSYNCPHCAAFEPQLEIWTRQLPSI
ASFRRVPVPFVGSDVEAKQRMYYALQALGKEDEFRPRIFRAIHQEHQRLFGDDAILAWADKQPGLDGKKFAEAYHSFSTM
TRAKRATQTTSDFKVAGVPALGIAGRWYADGETAGSPERALQVTSFLIREASRA

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=180, Percent_Identity=28.3333333333333, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 23840; Mature: 23840

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRHFLIAAAMVPLAPLANSVSAQEAFKAGKDFLELDKPVPVDAPPGKVEVIEFFSYNC
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCEEEEEECCCCC
PHCAAFEPQLEIWTRQLPSIASFRRVPVPFVGSDVEAKQRMYYALQALGKEDEFRPRIFR
CCCCCCCCHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
AIHQEHQRLFGDDAILAWADKQPGLDGKKFAEAYHSFSTMTRAKRATQTTSDFKVAGVPA
HHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
LGIAGRWYADGETAGSPERALQVTSFLIREASRA
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRRHFLIAAAMVPLAPLANSVSAQEAFKAGKDFLELDKPVPVDAPPGKVEVIEFFSYNC
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCEEEEEECCCCC
PHCAAFEPQLEIWTRQLPSIASFRRVPVPFVGSDVEAKQRMYYALQALGKEDEFRPRIFR
CCCCCCCCHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
AIHQEHQRLFGDDAILAWADKQPGLDGKKFAEAYHSFSTMTRAKRATQTTSDFKVAGVPA
HHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
LGIAGRWYADGETAGSPERALQVTSFLIREASRA
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10051655 [H]