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The map label for this gene is ngoBVM [H]

Identifier: 78047842

GI number: 78047842

Start: 2619215

End: 2620753

Strand: Reverse

Name: ngoBVM [H]

Synonym: XCV2286

Alternate gene names: 78047842

Gene position: 2620753-2619215 (Counterclockwise)

Preceding gene: 78047843

Following gene: 78047841

Centisome position: 50.61

GC content: 65.24

Gene sequence:

>1539_bases
ATGCACCCTCAACCTTGCGCGCCGCTGCTTTACGGCAGTGTGTGCAGCGGCATCGAGGCCGTGAGCCTAGCGTGGCAACC
GCTCGGCCTCGAAGCCGCGTGGTTCGCAGAGATCGAACCGTTCCCGAGCGCCGTGCTCGCCCACCACTACCCGCACGTGC
CCAACCTGGGCGACATGACCACGATCGCCCGCCAGGTTCACGCCGGCACCGTGCCGGCCCCCGACATCCTGGTCGGCGGC
ACGCCATGCCAGTCGTTCAGCGTGGCCGGCGCGCGCCGGGGACTGGATGACCCACGCGGAGCCTTGACCCTTGCCTATGT
GGAGTTAGCCAATGCCATCGACCAAGCCCGTCACCAAAATGACCGCTCGCCGGCCACCCTTGTCTGGGAAAACGTCCCGG
GCGTCCTCAGCGACCGCAGCAACGCCTTCGGGAACTTCCTGGGCGCATTGGCCGGAGAAAGCCGTGCGCTCCAACCGCCA
GGGAAAAAATGGGCGCACGCAGGTTACGTGTCTGGCCCCAGGCGCCGCATCGCCTGGCGCGTGCTCGACGCTCAATATTT
CGGCGTCGCCCAACGCCGCAAGCGTGTGTTTCTTGTGGCAAGTGGTGGAGATGGTTTCGATCCCGTCAAAGTACTTTTTG
AGCGCACGGGCCTGCGCGGGGATTCTTCTGCGGGCAGCGCGCCGTGGCAAGAAGCTGCCCGTGCTGCTGGACCAAGCGCT
GAAGCAGCAGGCGGATACGCAGGACTGAAATCGTCCTACGGTGAGGTCAAGACGACGTTCGGATTCAGTGGAGGCATCGG
CCCTGTCGATGTGGCCGCATGCCTAATGGCGGCTGGCCCCAAGCATGACATCCGCACGGAGACGTTCATGGTGCAGTCGG
TCGCCGGCAGCATCACCCACACCCTCGACACCGCCAACGGCGGCAAGGGCAGCAGCGAGGACGGTACGGGCAAAGGTGTG
CCGATCATCGCCTTCACCGCCCAGGGCAGCGGCGCGGATGCCACGATGGATCTGACGCCGACGCTGCGTGCAGGCGGGCA
TCGCAGCAGCCATGCGAACGCAGGTGTCGTGCCTGCTATCGCCTTCGCGCAGAACCACCGCTGCGAGGTGCGCTTCGAGT
CGGGCCACGGACAGGTGGCTTGCACCGTGCTGTCCAACGGCAAGCCGGGATATGGCGTGCCAATGATCGCCTGCGTTGCC
CTGCATGATCGTCAGAACGGTCTGGCTGCTGAACTGGGCAGCGGGGCCAATGGGCATGTGCTGGCGCCTGACCACGAGGC
GCATTTCCGCTATGACTGGAATGACCCCATTCCAAGGGACTGGTCGCAGTGGCGGGTGCGGCGGTTGATGCCCGTGGAAT
GCGAACGTCTGCAAGGCATGCCCGACGACTACACGCTGGTTCCGTATCGCGGTAAGCCGGCTGCGGACGCGCCGCGCTAC
AAGGCAATCGGCAACTCCATGGCCATGCCGTGCGTCGCCTGGCTAGGCCAGCGGCTGGTGCAGTGCTTGCACAAGATGGG
ATCGATCGCTTCGGATTGA

Upstream 100 bases:

>100_bases
CCTCCCGAGCCGAAGGTCCTACGGCCTTCGGCGCCTTTCTCCTGCCCATCAACGTCCCGGCCCGGCCATTGGCTTGGGTC
GTACGAACAGGAGAGCTGAT

Downstream 100 bases:

>100_bases
TGCACGACGCCGCCAACGGGCCGCGCCATTCTCGTTCCTGTATTGCCGACATGTCGGCAGCCGCCAGGGTGTCGAGGCTG
CCGTGGGTTGCACCCATGCC

Product: cytosine-specific DNA methyltransferase

Products: NA

Alternate protein names: M.NgoBV; Cytosine-specific methyltransferase NgoBV; M.NgoV [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MHPQPCAPLLYGSVCSGIEAVSLAWQPLGLEAAWFAEIEPFPSAVLAHHYPHVPNLGDMTTIARQVHAGTVPAPDILVGG
TPCQSFSVAGARRGLDDPRGALTLAYVELANAIDQARHQNDRSPATLVWENVPGVLSDRSNAFGNFLGALAGESRALQPP
GKKWAHAGYVSGPRRRIAWRVLDAQYFGVAQRRKRVFLVASGGDGFDPVKVLFERTGLRGDSSAGSAPWQEAARAAGPSA
EAAGGYAGLKSSYGEVKTTFGFSGGIGPVDVAACLMAAGPKHDIRTETFMVQSVAGSITHTLDTANGGKGSSEDGTGKGV
PIIAFTAQGSGADATMDLTPTLRAGGHRSSHANAGVVPAIAFAQNHRCEVRFESGHGQVACTVLSNGKPGYGVPMIACVA
LHDRQNGLAAELGSGANGHVLAPDHEAHFRYDWNDPIPRDWSQWRVRRLMPVECERLQGMPDDYTLVPYRGKPAADAPRY
KAIGNSMAMPCVAWLGQRLVQCLHKMGSIASD

Sequences:

>Translated_512_residues
MHPQPCAPLLYGSVCSGIEAVSLAWQPLGLEAAWFAEIEPFPSAVLAHHYPHVPNLGDMTTIARQVHAGTVPAPDILVGG
TPCQSFSVAGARRGLDDPRGALTLAYVELANAIDQARHQNDRSPATLVWENVPGVLSDRSNAFGNFLGALAGESRALQPP
GKKWAHAGYVSGPRRRIAWRVLDAQYFGVAQRRKRVFLVASGGDGFDPVKVLFERTGLRGDSSAGSAPWQEAARAAGPSA
EAAGGYAGLKSSYGEVKTTFGFSGGIGPVDVAACLMAAGPKHDIRTETFMVQSVAGSITHTLDTANGGKGSSEDGTGKGV
PIIAFTAQGSGADATMDLTPTLRAGGHRSSHANAGVVPAIAFAQNHRCEVRFESGHGQVACTVLSNGKPGYGVPMIACVA
LHDRQNGLAAELGSGANGHVLAPDHEAHFRYDWNDPIPRDWSQWRVRRLMPVECERLQGMPDDYTLVPYRGKPAADAPRY
KAIGNSMAMPCVAWLGQRLVQCLHKMGSIASD
>Mature_512_residues
MHPQPCAPLLYGSVCSGIEAVSLAWQPLGLEAAWFAEIEPFPSAVLAHHYPHVPNLGDMTTIARQVHAGTVPAPDILVGG
TPCQSFSVAGARRGLDDPRGALTLAYVELANAIDQARHQNDRSPATLVWENVPGVLSDRSNAFGNFLGALAGESRALQPP
GKKWAHAGYVSGPRRRIAWRVLDAQYFGVAQRRKRVFLVASGGDGFDPVKVLFERTGLRGDSSAGSAPWQEAARAAGPSA
EAAGGYAGLKSSYGEVKTTFGFSGGIGPVDVAACLMAAGPKHDIRTETFMVQSVAGSITHTLDTANGGKGSSEDGTGKGV
PIIAFTAQGSGADATMDLTPTLRAGGHRSSHANAGVVPAIAFAQNHRCEVRFESGHGQVACTVLSNGKPGYGVPMIACVA
LHDRQNGLAAELGSGANGHVLAPDHEAHFRYDWNDPIPRDWSQWRVRRLMPVECERLQGMPDDYTLVPYRGKPAADAPRY
KAIGNSMAMPCVAWLGQRLVQCLHKMGSIASD

Specific function: This methylase recognizes the double-stranded sequence GGNNCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NgoBV endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 54091; Mature: 54091

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPQPCAPLLYGSVCSGIEAVSLAWQPLGLEAAWFAEIEPFPSAVLAHHYPHVPNLGDMT
CCCCCCCHHHHHHHHCCCHHHHEEECCCCCCEEEEECCCCCHHHHHHHCCCCCCCCCHHH
TIARQVHAGTVPAPDILVGGTPCQSFSVAGARRGLDDPRGALTLAYVELANAIDQARHQN
HHHHHHHCCCCCCCCEEECCCCCCCCCHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHCC
DRSPATLVWENVPGVLSDRSNAFGNFLGALAGESRALQPPGKKWAHAGYVSGPRRRIAWR
CCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCEEEEE
VLDAQYFGVAQRRKRVFLVASGGDGFDPVKVLFERTGLRGDSSAGSAPWQEAARAAGPSA
EECHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCC
EAAGGYAGLKSSYGEVKTTFGFSGGIGPVDVAACLMAAGPKHDIRTETFMVQSVAGSITH
CCCCCHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
TLDTANGGKGSSEDGTGKGVPIIAFTAQGSGADATMDLTPTLRAGGHRSSHANAGVVPAI
EEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCCHHHCCCCCCCCCCCCCCHHH
AFAQNHRCEVRFESGHGQVACTVLSNGKPGYGVPMIACVALHDRQNGLAAELGSGANGHV
HHCCCCEEEEEEECCCCEEEEEEEECCCCCCCCHHEEEEEHHCCCCCCHHHHCCCCCCCE
LAPDHEAHFRYDWNDPIPRDWSQWRVRRLMPVECERLQGMPDDYTLVPYRGKPAADAPRY
ECCCCCCCEEECCCCCCCCCHHHHHHHHCCCCCHHHHCCCCCCEEEEEECCCCCCCCCCH
KAIGNSMAMPCVAWLGQRLVQCLHKMGSIASD
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MHPQPCAPLLYGSVCSGIEAVSLAWQPLGLEAAWFAEIEPFPSAVLAHHYPHVPNLGDMT
CCCCCCCHHHHHHHHCCCHHHHEEECCCCCCEEEEECCCCCHHHHHHHCCCCCCCCCHHH
TIARQVHAGTVPAPDILVGGTPCQSFSVAGARRGLDDPRGALTLAYVELANAIDQARHQN
HHHHHHHCCCCCCCCEEECCCCCCCCCHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHCC
DRSPATLVWENVPGVLSDRSNAFGNFLGALAGESRALQPPGKKWAHAGYVSGPRRRIAWR
CCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCEEEEE
VLDAQYFGVAQRRKRVFLVASGGDGFDPVKVLFERTGLRGDSSAGSAPWQEAARAAGPSA
EECHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCC
EAAGGYAGLKSSYGEVKTTFGFSGGIGPVDVAACLMAAGPKHDIRTETFMVQSVAGSITH
CCCCCHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
TLDTANGGKGSSEDGTGKGVPIIAFTAQGSGADATMDLTPTLRAGGHRSSHANAGVVPAI
EEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCCHHHCCCCCCCCCCCCCCHHH
AFAQNHRCEVRFESGHGQVACTVLSNGKPGYGVPMIACVALHDRQNGLAAELGSGANGHV
HHCCCCEEEEEEECCCCEEEEEEEECCCCCCCCHHEEEEEHHCCCCCCHHHHCCCCCCCE
LAPDHEAHFRYDWNDPIPRDWSQWRVRRLMPVECERLQGMPDDYTLVPYRGKPAADAPRY
ECCCCCCCEEECCCCCCCCCHHHHHHHHCCCCCHHHHCCCCCCEEEEEECCCCCCCCCCH
KAIGNSMAMPCVAWLGQRLVQCLHKMGSIASD
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7607490 [H]