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The map label for this gene is topB [H]

Identifier: 78047843

GI number: 78047843

Start: 2621288

End: 2623300

Strand: Reverse

Name: topB [H]

Synonym: XCV2287

Alternate gene names: 78047843

Gene position: 2623300-2621288 (Counterclockwise)

Preceding gene: 78047844

Following gene: 78047842

Centisome position: 50.66

GC content: 64.08

Gene sequence:

>2013_bases
ATGCGGCTGTTCTTGTGCGAGAAGCCTTCCCAGGGCAAAGACATTGGCCGGATTCTCGGCGCCACGCAACGCGGTGAAGG
CTGCCTCAACGGTTCCGGCGTCACCGTCACCTGGTGCATCGGCCATCTCGTAGAGGCGGCGGCACCCGAAGCCTACGACG
AACAGCTAAAGCGCTGGTCCATCGAGCAGTTGCCCATCATTCCCCAGCGTTGGCAGGTCGAGGTCAAACCGAAAACCGCC
ACGCAATTCAAGGTCGTCAAGGCGCTACTGGCGAAGGCAACACAGCTTGTCATCGCCACCGATGCCGACCGCGAAGGCGA
ACTGATCGCCCGCGAGATCATTGACCTGTGCGGCTACCGCGGCCCCATCGAGCGGCTGTGGTTGTCGGCACTCAACGATG
CGTCCATTCGCGCCGCGCTAGGCAAGCTCCGGCCATCGGCCGAGACGCTGCCGATGTACTACTCGGCACTAGCGCGCTCG
CGTGCGGACTGGCTTGTGGGCATGAACCTCAGTCGGTTGTTCACGGTGCTGGGTCGGCAAGCCGGCTACGACGGAGTGCT
GTCGGTCGGTCGCGTCCAGACCCCGACGCTCAAGCTGGTCGTGGACCGCGACCGCGAGATCGCGGCCTTCGTGTCCGTGC
CGTACTGGGCCATCGACGTGTACCTGACCACAAGCGGCCAAGCCTTCACCGCGCAGTGGATTTCGCCCGACACCTGCACC
GACGACTCGGGCCGCTGCCTGCAGCAGCCGGTGGCACAGCAGGCGGCGCAGCAGATCCGCGCCACCGGCAGTGCCCAGGT
TGTCTCGGTCGAAACCGAGCGCGTGCGTGAAGGCCCGCCGCTGCCGTTCGACCTGGGCACCTTGCAGGAGTTGTGTTCCA
AGCAGCTTGGGCTGGATGTGCAGGAAACCTTGGACATCGCTCAGGCCCTGTACGAAACGCACAAGGCCACGACCTATCCG
CGCTCGGATTCCGGCTACCTGCCGGAGAGCATGTTTGCCGAAGTCCCCATAGTCCTCGACAGCCTGCTCAAGACCGATTT
CTCGCTGCGCCCGATCATTGACCAACTCGACCGCACACAACGCTCACGCGCTTGGAACGACGCGAAGGTATCCGCGCACC
ACGGCATCATCCCGACGCTCGAACCGGCGAACCTCTCGGCCATGAGCGAGAAGGAACTGGCGGTGTACAAGCTCATCCGG
GCGCACTACCTCGCGCAGTTCCTTCCTCACCACGAGTTCGATCGCACAGTGGCGAACCTCTCTTGCGGCCAGCAGACGCT
GACGGCCACAGGAAAGCAGGTGGTCGTCAAGGGCTGGCGCCTGGTGTTGGCCGAACCGCAACCCGAGGAGGATAGCGACA
CCGCGGCGCGCAGCCAGGTGCTGCCCGCGCTACGCGAGGGCGTGTCATGCCAGGTAGCCGATGTCGATCTGAAGGCGCTC
AAGACACAGCCATCTCGACCCTACACGCAGGGCGAATTGGTCAAGTCCATGAAAGGCGTCGCCAAGCTGGTCTCGGATCC
GCGCTTGAAACAGAAGCTCAAGGATACGGTCGGCATCGGCACCGAAGCGACACGGGCTAACATCATCAGCGGCCTGATCG
CACGCGGCTATCTCATGAAGAAGGGGCGCGCGATTCGCGCCTCGGATGCGGCATTCACCCTCATCGACGCCGTGCCCGCA
GCGATTGCCGACCCCGGGACCACCGCCGTATGGGAACAAGCGCTAGACATGATCGAAGCGGGACAACTCACGCTGGACGT
GTTCATCGGCAAGCAGGCCGCATGGATTTCGCAGTTGATCACGCAGTACGCCAGCGCGTCCCTGTCAATCAAGGTTCCCC
ATGGGCCGGCTTGCCCGCAGTGCGGCGCACCCACGCACCAGCGCAGTGGCAAGAGCGGCCCGTTCTGGTCGTGCAGTCGC
TATCCAGACTGCAAAGGCACGCTGCCGGTCGAGTCCGGCACGTCCAAGCGCGTAGCCTCACGCTCGCGCCGTGGCGGCCG
AAAAGACTCCTGA

Upstream 100 bases:

>100_bases
TGGTCGCCACCGCATGCGGCTTGTTTGCTGCTGCTCTTGGTAGCGCTCGGCATCCTCGATTCCAGCACCTCAATGAACCA
CGGAAATCGGGTGGACGGAC

Downstream 100 bases:

>100_bases
TCGACCCCGTCCCCCCGTGAGCAGTGCTCGCCTTCGGCGGCGCGACTCGTGTCCCGCACGCCCTGCTGGACGCCCAGCGC
ACAACACCTTCTTGATCCGT

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 670; Mature: 670

Protein sequence:

>670_residues
MRLFLCEKPSQGKDIGRILGATQRGEGCLNGSGVTVTWCIGHLVEAAAPEAYDEQLKRWSIEQLPIIPQRWQVEVKPKTA
TQFKVVKALLAKATQLVIATDADREGELIAREIIDLCGYRGPIERLWLSALNDASIRAALGKLRPSAETLPMYYSALARS
RADWLVGMNLSRLFTVLGRQAGYDGVLSVGRVQTPTLKLVVDRDREIAAFVSVPYWAIDVYLTTSGQAFTAQWISPDTCT
DDSGRCLQQPVAQQAAQQIRATGSAQVVSVETERVREGPPLPFDLGTLQELCSKQLGLDVQETLDIAQALYETHKATTYP
RSDSGYLPESMFAEVPIVLDSLLKTDFSLRPIIDQLDRTQRSRAWNDAKVSAHHGIIPTLEPANLSAMSEKELAVYKLIR
AHYLAQFLPHHEFDRTVANLSCGQQTLTATGKQVVVKGWRLVLAEPQPEEDSDTAARSQVLPALREGVSCQVADVDLKAL
KTQPSRPYTQGELVKSMKGVAKLVSDPRLKQKLKDTVGIGTEATRANIISGLIARGYLMKKGRAIRASDAAFTLIDAVPA
AIADPGTTAVWEQALDMIEAGQLTLDVFIGKQAAWISQLITQYASASLSIKVPHGPACPQCGAPTHQRSGKSGPFWSCSR
YPDCKGTLPVESGTSKRVASRSRRGGRKDS

Sequences:

>Translated_670_residues
MRLFLCEKPSQGKDIGRILGATQRGEGCLNGSGVTVTWCIGHLVEAAAPEAYDEQLKRWSIEQLPIIPQRWQVEVKPKTA
TQFKVVKALLAKATQLVIATDADREGELIAREIIDLCGYRGPIERLWLSALNDASIRAALGKLRPSAETLPMYYSALARS
RADWLVGMNLSRLFTVLGRQAGYDGVLSVGRVQTPTLKLVVDRDREIAAFVSVPYWAIDVYLTTSGQAFTAQWISPDTCT
DDSGRCLQQPVAQQAAQQIRATGSAQVVSVETERVREGPPLPFDLGTLQELCSKQLGLDVQETLDIAQALYETHKATTYP
RSDSGYLPESMFAEVPIVLDSLLKTDFSLRPIIDQLDRTQRSRAWNDAKVSAHHGIIPTLEPANLSAMSEKELAVYKLIR
AHYLAQFLPHHEFDRTVANLSCGQQTLTATGKQVVVKGWRLVLAEPQPEEDSDTAARSQVLPALREGVSCQVADVDLKAL
KTQPSRPYTQGELVKSMKGVAKLVSDPRLKQKLKDTVGIGTEATRANIISGLIARGYLMKKGRAIRASDAAFTLIDAVPA
AIADPGTTAVWEQALDMIEAGQLTLDVFIGKQAAWISQLITQYASASLSIKVPHGPACPQCGAPTHQRSGKSGPFWSCSR
YPDCKGTLPVESGTSKRVASRSRRGGRKDS
>Mature_670_residues
MRLFLCEKPSQGKDIGRILGATQRGEGCLNGSGVTVTWCIGHLVEAAAPEAYDEQLKRWSIEQLPIIPQRWQVEVKPKTA
TQFKVVKALLAKATQLVIATDADREGELIAREIIDLCGYRGPIERLWLSALNDASIRAALGKLRPSAETLPMYYSALARS
RADWLVGMNLSRLFTVLGRQAGYDGVLSVGRVQTPTLKLVVDRDREIAAFVSVPYWAIDVYLTTSGQAFTAQWISPDTCT
DDSGRCLQQPVAQQAAQQIRATGSAQVVSVETERVREGPPLPFDLGTLQELCSKQLGLDVQETLDIAQALYETHKATTYP
RSDSGYLPESMFAEVPIVLDSLLKTDFSLRPIIDQLDRTQRSRAWNDAKVSAHHGIIPTLEPANLSAMSEKELAVYKLIR
AHYLAQFLPHHEFDRTVANLSCGQQTLTATGKQVVVKGWRLVLAEPQPEEDSDTAARSQVLPALREGVSCQVADVDLKAL
KTQPSRPYTQGELVKSMKGVAKLVSDPRLKQKLKDTVGIGTEATRANIISGLIARGYLMKKGRAIRASDAAFTLIDAVPA
AIADPGTTAVWEQALDMIEAGQLTLDVFIGKQAAWISQLITQYASASLSIKVPHGPACPQCGAPTHQRSGKSGPFWSCSR
YPDCKGTLPVESGTSKRVASRSRRGGRKDS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=712, Percent_Identity=24.0168539325843, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI4507635, Length=677, Percent_Identity=22.8951255539143, Blast_Score=107, Evalue=4e-23,
Organism=Escherichia coli, GI1788061, Length=614, Percent_Identity=40.228013029316, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1787529, Length=678, Percent_Identity=25.5162241887906, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI32563869, Length=586, Percent_Identity=24.5733788395904, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17555378, Length=648, Percent_Identity=24.8456790123457, Blast_Score=120, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6323263, Length=608, Percent_Identity=25.8223684210526, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24585251, Length=725, Percent_Identity=25.5172413793103, Blast_Score=131, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24640096, Length=579, Percent_Identity=23.6614853195164, Blast_Score=102, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 73136; Mature: 73136

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFLCEKPSQGKDIGRILGATQRGEGCLNGSGVTVTWCIGHLVEAAAPEAYDEQLKRWS
CEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCHHHHHHHHHCC
IEQLPIIPQRWQVEVKPKTATQFKVVKALLAKATQLVIATDADREGELIAREIIDLCGYR
HHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHCCC
GPIERLWLSALNDASIRAALGKLRPSAETLPMYYSALARSRADWLVGMNLSRLFTVLGRQ
CHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHC
AGYDGVLSVGRVQTPTLKLVVDRDREIAAFVSVPYWAIDVYLTTSGQAFTAQWISPDTCT
CCCCHHHCCCCCCCCEEEEEEECCCCEEEEEECCEEEEEEEEECCCCEEEEECCCCCCCC
DDSGRCLQQPVAQQAAQQIRATGSAQVVSVETERVREGPPLPFDLGTLQELCSKQLGLDV
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHCCCH
QETLDIAQALYETHKATTYPRSDSGYLPESMFAEVPIVLDSLLKTDFSLRPIIDQLDRTQ
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
RSRAWNDAKVSAHHGIIPTLEPANLSAMSEKELAVYKLIRAHYLAQFLPHHEFDRTVANL
HHCCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SCGQQTLTATGKQVVVKGWRLVLAEPQPEEDSDTAARSQVLPALREGVSCQVADVDLKAL
HCCCHHHHHCCCCEEEECEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHH
KTQPSRPYTQGELVKSMKGVAKLVSDPRLKQKLKDTVGIGTEATRANIISGLIARGYLMK
CCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
KGRAIRASDAAFTLIDAVPAAIADPGTTAVWEQALDMIEAGQLTLDVFIGKQAAWISQLI
CCCEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHH
TQYASASLSIKVPHGPACPQCGAPTHQRSGKSGPFWSCSRYPDCKGTLPVESGTSKRVAS
HHHHCCCEEEECCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
RSRRGGRKDS
HHHCCCCCCC
>Mature Secondary Structure
MRLFLCEKPSQGKDIGRILGATQRGEGCLNGSGVTVTWCIGHLVEAAAPEAYDEQLKRWS
CEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCHHHHHHHHHCC
IEQLPIIPQRWQVEVKPKTATQFKVVKALLAKATQLVIATDADREGELIAREIIDLCGYR
HHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHCCC
GPIERLWLSALNDASIRAALGKLRPSAETLPMYYSALARSRADWLVGMNLSRLFTVLGRQ
CHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHC
AGYDGVLSVGRVQTPTLKLVVDRDREIAAFVSVPYWAIDVYLTTSGQAFTAQWISPDTCT
CCCCHHHCCCCCCCCEEEEEEECCCCEEEEEECCEEEEEEEEECCCCEEEEECCCCCCCC
DDSGRCLQQPVAQQAAQQIRATGSAQVVSVETERVREGPPLPFDLGTLQELCSKQLGLDV
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHCCCH
QETLDIAQALYETHKATTYPRSDSGYLPESMFAEVPIVLDSLLKTDFSLRPIIDQLDRTQ
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
RSRAWNDAKVSAHHGIIPTLEPANLSAMSEKELAVYKLIRAHYLAQFLPHHEFDRTVANL
HHCCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SCGQQTLTATGKQVVVKGWRLVLAEPQPEEDSDTAARSQVLPALREGVSCQVADVDLKAL
HCCCHHHHHCCCCEEEECEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHH
KTQPSRPYTQGELVKSMKGVAKLVSDPRLKQKLKDTVGIGTEATRANIISGLIARGYLMK
CCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
KGRAIRASDAAFTLIDAVPAAIADPGTTAVWEQALDMIEAGQLTLDVFIGKQAAWISQLI
CCCEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHH
TQYASASLSIKVPHGPACPQCGAPTHQRSGKSGPFWSCSRYPDCKGTLPVESGTSKRVAS
HHHHCCCEEEECCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
RSRRGGRKDS
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2553698; 9097039; 9278503; 10574789 [H]