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The map label for this gene is mltD [H]

Identifier: 78046642

GI number: 78046642

Start: 1222161

End: 1223339

Strand: Reverse

Name: mltD [H]

Synonym: XCV1086

Alternate gene names: 78046642

Gene position: 1223339-1222161 (Counterclockwise)

Preceding gene: 78046643

Following gene: 78046635

Centisome position: 23.62

GC content: 67.18

Gene sequence:

>1179_bases
ATGAGGCGCCTGGTTTTGACAGTGCTGGCCGCCTGTTCGGCCATCGCACCGGCATCCGCATCGCCCAACGACGCAAGCTC
CGCCCCCGCCAGTAGCCAGGTCGCGCAAGCCAGCGCGCCAAGCCTCCGCAACGGGCGCGAGATTTTTTCGGCCTTCCTCG
ACGGTCGTGCCGACCCGGGCTGCGACAGCGCCCATAGCGATGCCCGCTGGGAACAGCATTTTTCGCGCGCCCCGGCACGT
CTGGCAGACGATGCGCAGGATGTCCTGCCGTTATTCGGTTATGTCGTCGACGAGTTGCGCAAGGCCGACATGCCGACCGA
ATTTGCGCTGATCCCGTTCGTTGAAAGCGGCTATCGCCCCGGGGCCCGCAACGGCAGCGGCCCTGCCGGCCTGTGGCAGT
TCATCGCGACCACCGCGCGCAACCACCAGGTGCCGGTCGGTGCGCAATATGACGGACGCCTGTCGGCAGTGGACTCCACC
ACTGCGGCGGTGCGCTATCTCAAAACCCTTTACGGCATGTTCGGCGGAGACTGGCGCCTGGCCCTGATGGCCTACAACGC
CGGCGAGTACCGCGTGCTGCAGGCAATGCGCAGCGCCGGGATGAACGCGCAAAACGCACGCCCCTCCGAATTGCCGGGCA
TGTCCAAGGTCACCTACGACTATGTGGAGAAACTGCACGCACTTGCCTGCGTGCTGGATCACGCGCAACAGCAGGGCAAC
CTGCTGACGTCGCTGGATCGCCAGGTGCCCGTCCTCACCGAACATGCGTTGCCGGCAGGCACCAGCCTCAACGCCTGGGC
CGGACAGCGCGCGATCGAGCCGCAACTGCTCGCGCGCCTGAATCCCGCCCTCGCTGGCGCACGCGCTGCGCCGCGCGGCG
TGCACGTGCTTGCGCCGCTTGCGCCGCCGCAGCCCGGTGCAAATAGCGCCATGGTCGCCTCTGCCGAGGTGGGAACTGGC
GCCGCCAGCAGCACCCCCGCACCGACCGCCGTTGCCTCTGCAGCCAGCAAAGCGTCGACCTCGCCAGCACGTACCCACAC
CGTGCGCAATGGCGAATCGGCCTGGGCGATCGCGCGCCGCTATGGCATCACCGTGACGACGCTGCTCGCCACCAACGGCC
TGGACAAAAGCGCCATTCTGAAGCCTGGCATGGTGCTGTCGTTCGACGACTCGCCCTAA

Upstream 100 bases:

>100_bases
CCGAGATCCGCGCTGCAGTGGCACCGCGCGCAGCCGGCGAGCTTTCCTCTGAAGTCGATGTTTTCGCCGAACTTCGGCGC
TGGAAAGACGAATTTCGCTC

Downstream 100 bases:

>100_bases
AGTGACGCGCCAAGGTGCGACGCACCTTGGACGTTGTCGTGGGCACAGGCAGGCGGTAGGCGGTAGGCGGTAGGCGGTAG
GCGGTAGGCGGCATGATCGC

Product: membrane-bound murein hydrolase D

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MRRLVLTVLAACSAIAPASASPNDASSAPASSQVAQASAPSLRNGREIFSAFLDGRADPGCDSAHSDARWEQHFSRAPAR
LADDAQDVLPLFGYVVDELRKADMPTEFALIPFVESGYRPGARNGSGPAGLWQFIATTARNHQVPVGAQYDGRLSAVDST
TAAVRYLKTLYGMFGGDWRLALMAYNAGEYRVLQAMRSAGMNAQNARPSELPGMSKVTYDYVEKLHALACVLDHAQQQGN
LLTSLDRQVPVLTEHALPAGTSLNAWAGQRAIEPQLLARLNPALAGARAAPRGVHVLAPLAPPQPGANSAMVASAEVGTG
AASSTPAPTAVASAASKASTSPARTHTVRNGESAWAIARRYGITVTTLLATNGLDKSAILKPGMVLSFDDSP

Sequences:

>Translated_392_residues
MRRLVLTVLAACSAIAPASASPNDASSAPASSQVAQASAPSLRNGREIFSAFLDGRADPGCDSAHSDARWEQHFSRAPAR
LADDAQDVLPLFGYVVDELRKADMPTEFALIPFVESGYRPGARNGSGPAGLWQFIATTARNHQVPVGAQYDGRLSAVDST
TAAVRYLKTLYGMFGGDWRLALMAYNAGEYRVLQAMRSAGMNAQNARPSELPGMSKVTYDYVEKLHALACVLDHAQQQGN
LLTSLDRQVPVLTEHALPAGTSLNAWAGQRAIEPQLLARLNPALAGARAAPRGVHVLAPLAPPQPGANSAMVASAEVGTG
AASSTPAPTAVASAASKASTSPARTHTVRNGESAWAIARRYGITVTTLLATNGLDKSAILKPGMVLSFDDSP
>Mature_392_residues
MRRLVLTVLAACSAIAPASASPNDASSAPASSQVAQASAPSLRNGREIFSAFLDGRADPGCDSAHSDARWEQHFSRAPAR
LADDAQDVLPLFGYVVDELRKADMPTEFALIPFVESGYRPGARNGSGPAGLWQFIATTARNHQVPVGAQYDGRLSAVDST
TAAVRYLKTLYGMFGGDWRLALMAYNAGEYRVLQAMRSAGMNAQNARPSELPGMSKVTYDYVEKLHALACVLDHAQQQGN
LLTSLDRQVPVLTEHALPAGTSLNAWAGQRAIEPQLLARLNPALAGARAAPRGVHVLAPLAPPQPGANSAMVASAEVGTG
AASSTPAPTAVASAASKASTSPARTHTVRNGESAWAIARRYGITVTTLLATNGLDKSAILKPGMVLSFDDSP

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=323, Percent_Identity=29.7213622291022, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 41065; Mature: 41065

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLVLTVLAACSAIAPASASPNDASSAPASSQVAQASAPSLRNGREIFSAFLDGRADPG
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCC
CDSAHSDARWEQHFSRAPARLADDAQDVLPLFGYVVDELRKADMPTEFALIPFVESGYRP
CCCCCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHCCCCC
GARNGSGPAGLWQFIATTARNHQVPVGAQYDGRLSAVDSTTAAVRYLKTLYGMFGGDWRL
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEE
ALMAYNAGEYRVLQAMRSAGMNAQNARPSELPGMSKVTYDYVEKLHALACVLDHAQQQGN
EEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
LLTSLDRQVPVLTEHALPAGTSLNAWAGQRAIEPQLLARLNPALAGARAAPRGVHVLAPL
HHHHHHCCCCHHHHCCCCCCCCCCHHCCCCCCCHHHHHHHCHHHHCCCCCCCCEEEEECC
APPQPGANSAMVASAEVGTGAASSTPAPTAVASAASKASTSPARTHTVRNGESAWAIARR
CCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHEECCCCHHHHHHHH
YGITVTTLLATNGLDKSAILKPGMVLSFDDSP
HCCEEEEEEHHCCCCHHHHCCCCEEEEECCCC
>Mature Secondary Structure
MRRLVLTVLAACSAIAPASASPNDASSAPASSQVAQASAPSLRNGREIFSAFLDGRADPG
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCC
CDSAHSDARWEQHFSRAPARLADDAQDVLPLFGYVVDELRKADMPTEFALIPFVESGYRP
CCCCCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHCCCCC
GARNGSGPAGLWQFIATTARNHQVPVGAQYDGRLSAVDSTTAAVRYLKTLYGMFGGDWRL
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEE
ALMAYNAGEYRVLQAMRSAGMNAQNARPSELPGMSKVTYDYVEKLHALACVLDHAQQQGN
EEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
LLTSLDRQVPVLTEHALPAGTSLNAWAGQRAIEPQLLARLNPALAGARAAPRGVHVLAPL
HHHHHHCCCCHHHHCCCCCCCCCCHHCCCCCCCHHHHHHHCHHHHCCCCCCCCEEEEECC
APPQPGANSAMVASAEVGTGAASSTPAPTAVASAASKASTSPARTHTVRNGESAWAIARR
CCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHEECCCCHHHHHHHH
YGITVTTLLATNGLDKSAILKPGMVLSFDDSP
HCCEEEEEEHHCCCCHHHHCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]