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The map label for this gene is dusB [H]

Identifier: 78046424

GI number: 78046424

Start: 996846

End: 997844

Strand: Reverse

Name: dusB [H]

Synonym: XCV0868

Alternate gene names: 78046424

Gene position: 997844-996846 (Counterclockwise)

Preceding gene: 78046425

Following gene: 78046423

Centisome position: 19.27

GC content: 66.47

Gene sequence:

>999_bases
ATGCAGATCGGCTCCTACTCCATCGCTCCCAAGGTGATCCTCGCCCCCATGGCGGGCGTTACCGACAAACCGTTCCGGCT
GCTGTGCAAGCGGCTGGGCGCGGGCCTGGCGGTGTCGGAAATGACCATTTCCGACCCGCGCTTCTGGGGCACGCGCAAAT
CGCTGCACCGCATGGACCATGCCGGCGAGCCGGACCCGATCAGCGTGCAGATCGCCGGCACCGAGCCGCAGCAACTGGCC
GAGGCGGCGCGCTACAACGTCGACCACGGTGCGCAGTTGATCGACATCAACATGGGCTGCCCGGCCAAGAAGGTCTGCAA
CGCGTGGGCCGGCTCGGCGCTGATGCGCGACGAAGACCTGGTGGCACGCATCCTGACCGCGGTGGTACAGGCAGTGAACG
TGCCGGTGACGTTGAAGATCCGCACCGGTTGGGATTGCGACCACCGCAACGGCCCGACGATCGCGCGGATCGCGCAGGAC
TGCGGCATCGCCGCGCTCGCCGTACACGGACGCACCCGCGACCAGCATTACACCGGCAGCGCCGAGTACGCGACGATTGC
GCAGATCAAGGCCGCGCTGCAGATCCCGGTGGTTGCCAATGGCGATATCGACTCGCCGCAGAAAGCCGCTCAGGTGCTGC
GCGAAACCGGCGCCGATGCCGTGATGATCGGCCGCGCCGCACAAGGCCGCCCGTGGATCTTCGGCGAAGTGGCACATTTT
TTGTCCACCGGCGAGGTATTGCCGCCGCCGTCGTTGGCGTTCGTGCGCGATACCTTGCTCGGCCACCTGGAAGCGCTGCA
CGCGTTTTATGGCCAACCGCAGGGCGTGCGCATCGCACGCAAGCATCTGGGTTGGTATGCCAAGGACCACCCGCAAAGCG
CCGACTTCCGGGCGGTGGTCAATCGCGCCGAAACGCCCGAAGCCCAGTTGGCCCTGACGCGTGATTACTTCGATGCCTTG
ATCGCCGGCGTGCCGCCCGCACTATCGGACGCTGCCTGA

Upstream 100 bases:

>100_bases
CACAGCGGCGGTGCCGGCCATGCGGCCCGCCTGCTGCCGGCCCGGCCGCCGCAGGGTTGGCAGCGCGAAGGGCCGTCACC
GCTGAAGTTACACTAGCGCC

Downstream 100 bases:

>100_bases
GTTTTCCTGCCTTGCTGGAGCCGCACGCATGAGCGCACCGAGCGACACCCCCACCTATCTGCACGGCTTTTCCGCCACCG
AACAGGCGCGTCTGCTCAAA

Product: nifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MQIGSYSIAPKVILAPMAGVTDKPFRLLCKRLGAGLAVSEMTISDPRFWGTRKSLHRMDHAGEPDPISVQIAGTEPQQLA
EAARYNVDHGAQLIDINMGCPAKKVCNAWAGSALMRDEDLVARILTAVVQAVNVPVTLKIRTGWDCDHRNGPTIARIAQD
CGIAALAVHGRTRDQHYTGSAEYATIAQIKAALQIPVVANGDIDSPQKAAQVLRETGADAVMIGRAAQGRPWIFGEVAHF
LSTGEVLPPPSLAFVRDTLLGHLEALHAFYGQPQGVRIARKHLGWYAKDHPQSADFRAVVNRAETPEAQLALTRDYFDAL
IAGVPPALSDAA

Sequences:

>Translated_332_residues
MQIGSYSIAPKVILAPMAGVTDKPFRLLCKRLGAGLAVSEMTISDPRFWGTRKSLHRMDHAGEPDPISVQIAGTEPQQLA
EAARYNVDHGAQLIDINMGCPAKKVCNAWAGSALMRDEDLVARILTAVVQAVNVPVTLKIRTGWDCDHRNGPTIARIAQD
CGIAALAVHGRTRDQHYTGSAEYATIAQIKAALQIPVVANGDIDSPQKAAQVLRETGADAVMIGRAAQGRPWIFGEVAHF
LSTGEVLPPPSLAFVRDTLLGHLEALHAFYGQPQGVRIARKHLGWYAKDHPQSADFRAVVNRAETPEAQLALTRDYFDAL
IAGVPPALSDAA
>Mature_332_residues
MQIGSYSIAPKVILAPMAGVTDKPFRLLCKRLGAGLAVSEMTISDPRFWGTRKSLHRMDHAGEPDPISVQIAGTEPQQLA
EAARYNVDHGAQLIDINMGCPAKKVCNAWAGSALMRDEDLVARILTAVVQAVNVPVTLKIRTGWDCDHRNGPTIARIAQD
CGIAALAVHGRTRDQHYTGSAEYATIAQIKAALQIPVVANGDIDSPQKAAQVLRETGADAVMIGRAAQGRPWIFGEVAHF
LSTGEVLPPPSLAFVRDTLLGHLEALHAFYGQPQGVRIARKHLGWYAKDHPQSADFRAVVNRAETPEAQLALTRDYFDAL
IAGVPPALSDAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=238, Percent_Identity=32.3529411764706, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI239788462, Length=238, Percent_Identity=32.3529411764706, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI40807366, Length=229, Percent_Identity=30.5676855895196, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI8923374, Length=242, Percent_Identity=31.8181818181818, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI31742496, Length=227, Percent_Identity=30.8370044052863, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1789660, Length=318, Percent_Identity=55.9748427672956, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1788462, Length=233, Percent_Identity=36.480686695279, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI145693211, Length=318, Percent_Identity=26.7295597484277, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17510279, Length=244, Percent_Identity=32.3770491803279, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17543114, Length=238, Percent_Identity=28.5714285714286, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17507177, Length=243, Percent_Identity=27.9835390946502, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25144369, Length=179, Percent_Identity=30.7262569832402, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=236, Percent_Identity=31.7796610169492, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323433, Length=255, Percent_Identity=30.1960784313725, Blast_Score=102, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6323437, Length=233, Percent_Identity=26.6094420600858, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24585320, Length=234, Percent_Identity=32.9059829059829, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24580595, Length=273, Percent_Identity=32.2344322344322, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI19920448, Length=273, Percent_Identity=32.2344322344322, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI19921524, Length=229, Percent_Identity=27.0742358078603, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45549423, Length=243, Percent_Identity=30.8641975308642, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35709; Mature: 35709

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGSYSIAPKVILAPMAGVTDKPFRLLCKRLGAGLAVSEMTISDPRFWGTRKSLHRMDH
CCCCCCCCCCHHEECCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHC
AGEPDPISVQIAGTEPQQLAEAARYNVDHGAQLIDINMGCPAKKVCNAWAGSALMRDEDL
CCCCCCEEEEEECCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCHHCCCHHH
VARILTAVVQAVNVPVTLKIRTGWDCDHRNGPTIARIAQDCGIAALAVHGRTRDQHYTGS
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHCCEEEEEECCCCCCCCCCCC
AEYATIAQIKAALQIPVVANGDIDSPQKAAQVLRETGADAVMIGRAAQGRPWIFGEVAHF
CCHHHHHHHHHHHEECEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
LSTGEVLPPPSLAFVRDTLLGHLEALHAFYGQPQGVRIARKHLGWYAKDHPQSADFRAVV
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCHHHHHH
NRAETPEAQLALTRDYFDALIAGVPPALSDAA
HCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MQIGSYSIAPKVILAPMAGVTDKPFRLLCKRLGAGLAVSEMTISDPRFWGTRKSLHRMDH
CCCCCCCCCCHHEECCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHC
AGEPDPISVQIAGTEPQQLAEAARYNVDHGAQLIDINMGCPAKKVCNAWAGSALMRDEDL
CCCCCCEEEEEECCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCHHCCCHHH
VARILTAVVQAVNVPVTLKIRTGWDCDHRNGPTIARIAQDCGIAALAVHGRTRDQHYTGS
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHCCEEEEEECCCCCCCCCCCC
AEYATIAQIKAALQIPVVANGDIDSPQKAAQVLRETGADAVMIGRAAQGRPWIFGEVAHF
CCHHHHHHHHHHHEECEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
LSTGEVLPPPSLAFVRDTLLGHLEALHAFYGQPQGVRIARKHLGWYAKDHPQSADFRAVV
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCHHHHHH
NRAETPEAQLALTRDYFDALIAGVPPALSDAA
HCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]