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The map label for this gene is cca [H]

Identifier: 78046329

GI number: 78046329

Start: 883052

End: 884284

Strand: Reverse

Name: cca [H]

Synonym: XCV0773

Alternate gene names: 78046329

Gene position: 884284-883052 (Counterclockwise)

Preceding gene: 78046330

Following gene: 78046308

Centisome position: 17.08

GC content: 66.26

Gene sequence:

>1233_bases
ATGAAGATCTATCTCGTTGGCGGCGCAGTCCGCGATGCATTGCTTGAGCAGCCGGCTGGCGACCGCGACTGGGTGGTGGT
TGGCGCCGATCAGGCGCAGATGGAAGCGCAGGGGTTCAAGCCGGTCGGCAAGGATTTCCCGGTGTTCCTGCACCCGCGCA
GTGGCGAGGAATACGCACTGGCGCGTACCGAGCGCAAATCCGGCCGCGGATATCGCGGCTTCGTGGTGGACGCCGACCCA
TCGGTGACCCTGGAAGAAGACCTGCTACGCCGCGACTTCACCATCAACGCGATCGCGCGCGATGAGGAGACGGGTGAATT
GTTCGACCCGTACAACGGTGCGCGTGATCTGCAGGCGCGCGTCTTACGGCATGTCGGTCCGGCGTTTGTCGAAGATCCTG
TGCGCGTGTTGCGTGCCGCACGCTTCATGGCGCGGCTGGCACCGCTGGGCTTCACGCTCGCTGCCGACACTGCGGCTTTG
ATGCGGGAGATGGCCGCCAGCGGCGAACTCGATAGCCTGGTGCCGGAGCGCGTGTGGCAGGAGCTGCGGCGCGCATTGAG
CTGCGCGCAGCCCTCGGCGTTCCTGCGCACGCTGCACGATGCCGATGCGCTGCGGGTCATCCTGCCGGAAGTCGATGCGC
TGTACGGCGTGCCGCAGCGCGCCGAATTCCATCCGGAGGTCGACACGGGCATTCACCAGGAAATGGTCAGCGATATGGCG
GCACGTCTCGCGCCCGGCGATGCGCTGGTGGGCTTTGCCGCACTGACCCACGATCTGGGCAAGGCACTCACACCACCTGA
GGAATGGCCGCGCCACGTCATGCACGAACAACGCGGCGTGGCGCCATTGCAGGCGCTGTGCGAGCGGCTCAAGGTGCCGC
AGGACTTTCGGCAACTTGCCATCATCGCCTGCCGCGAACACTTGAACGTGCATCGCCTGGCCGAGCTGCGCGACCGCACC
CTGCACGAGCTGCTAGTGCGCTGCGATGCGTTTCGCCGCCCGGAGCGTATCGCGCAGCTGGCACTGGTCTGCGAAGCCGA
CAAGCGTGGCCGTCTGGGCAGTGAAGAAGCCGCGTACCCGCAGGGCGAAGCACTCAAACGGCTACATGCCGCCGCACTGG
CGATCAATGCGCGGGATCTGGCGGCCGAAGGTCTGCAAGGGCCGCAGATCGGCGAGGCACTGACAAAGGCGCGCATTGCC
GCGATTGCGGCAGCGCGACGGATCGGTGCATAG

Upstream 100 bases:

>100_bases
CTTCCATGAGCGCGTTGGCGGCGTCCTCGCCGCCGGTGGTCCCGCAAGCAGCCGGGAAAACGCACAGACAGGCGCATGAT
TGCTCGGGCATCATGCGCTG

Downstream 100 bases:

>100_bases
CTGCATGTCGCGCACCTGGGTCCAGGTGCGACGCGATGTGGGGACGCCAGGAAGGACACAATTGCAGCACCCCATGGACC
GAAACCTGCCCATTCGATGT

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKIYLVGGAVRDALLEQPAGDRDWVVVGADQAQMEAQGFKPVGKDFPVFLHPRSGEEYALARTERKSGRGYRGFVVDADP
SVTLEEDLLRRDFTINAIARDEETGELFDPYNGARDLQARVLRHVGPAFVEDPVRVLRAARFMARLAPLGFTLAADTAAL
MREMAASGELDSLVPERVWQELRRALSCAQPSAFLRTLHDADALRVILPEVDALYGVPQRAEFHPEVDTGIHQEMVSDMA
ARLAPGDALVGFAALTHDLGKALTPPEEWPRHVMHEQRGVAPLQALCERLKVPQDFRQLAIIACREHLNVHRLAELRDRT
LHELLVRCDAFRRPERIAQLALVCEADKRGRLGSEEAAYPQGEALKRLHAAALAINARDLAAEGLQGPQIGEALTKARIA
AIAAARRIGA

Sequences:

>Translated_410_residues
MKIYLVGGAVRDALLEQPAGDRDWVVVGADQAQMEAQGFKPVGKDFPVFLHPRSGEEYALARTERKSGRGYRGFVVDADP
SVTLEEDLLRRDFTINAIARDEETGELFDPYNGARDLQARVLRHVGPAFVEDPVRVLRAARFMARLAPLGFTLAADTAAL
MREMAASGELDSLVPERVWQELRRALSCAQPSAFLRTLHDADALRVILPEVDALYGVPQRAEFHPEVDTGIHQEMVSDMA
ARLAPGDALVGFAALTHDLGKALTPPEEWPRHVMHEQRGVAPLQALCERLKVPQDFRQLAIIACREHLNVHRLAELRDRT
LHELLVRCDAFRRPERIAQLALVCEADKRGRLGSEEAAYPQGEALKRLHAAALAINARDLAAEGLQGPQIGEALTKARIA
AIAAARRIGA
>Mature_410_residues
MKIYLVGGAVRDALLEQPAGDRDWVVVGADQAQMEAQGFKPVGKDFPVFLHPRSGEEYALARTERKSGRGYRGFVVDADP
SVTLEEDLLRRDFTINAIARDEETGELFDPYNGARDLQARVLRHVGPAFVEDPVRVLRAARFMARLAPLGFTLAADTAAL
MREMAASGELDSLVPERVWQELRRALSCAQPSAFLRTLHDADALRVILPEVDALYGVPQRAEFHPEVDTGIHQEMVSDMA
ARLAPGDALVGFAALTHDLGKALTPPEEWPRHVMHEQRGVAPLQALCERLKVPQDFRQLAIIACREHLNVHRLAELRDRT
LHELLVRCDAFRRPERIAQLALVCEADKRGRLGSEEAAYPQGEALKRLHAAALAINARDLAAEGLQGPQIGEALTKARIA
AIAAARRIGA

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=406, Percent_Identity=55.4187192118227, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI87081691, Length=255, Percent_Identity=27.843137254902, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45195; Mature: 45195

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYLVGGAVRDALLEQPAGDRDWVVVGADQAQMEAQGFKPVGKDFPVFLHPRSGEEYAL
CEEEEECHHHHHHHHHCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCEEEECCCCCCHHH
ARTERKSGRGYRGFVVDADPSVTLEEDLLRRDFTINAIARDEETGELFDPYNGARDLQAR
HHHHHHCCCCCEEEEEECCCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHH
VLRHVGPAFVEDPVRVLRAARFMARLAPLGFTLAADTAALMREMAASGELDSLVPERVWQ
HHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ELRRALSCAQPSAFLRTLHDADALRVILPEVDALYGVPQRAEFHPEVDTGIHQEMVSDMA
HHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
ARLAPGDALVGFAALTHDLGKALTPPEEWPRHVMHEQRGVAPLQALCERLKVPQDFRQLA
HHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHH
IIACREHLNVHRLAELRDRTLHELLVRCDAFRRPERIAQLALVCEADKRGRLGSEEAAYP
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
QGEALKRLHAAALAINARDLAAEGLQGPQIGEALTKARIAAIAAARRIGA
CHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIYLVGGAVRDALLEQPAGDRDWVVVGADQAQMEAQGFKPVGKDFPVFLHPRSGEEYAL
CEEEEECHHHHHHHHHCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCEEEECCCCCCHHH
ARTERKSGRGYRGFVVDADPSVTLEEDLLRRDFTINAIARDEETGELFDPYNGARDLQAR
HHHHHHCCCCCEEEEEECCCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHH
VLRHVGPAFVEDPVRVLRAARFMARLAPLGFTLAADTAALMREMAASGELDSLVPERVWQ
HHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ELRRALSCAQPSAFLRTLHDADALRVILPEVDALYGVPQRAEFHPEVDTGIHQEMVSDMA
HHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
ARLAPGDALVGFAALTHDLGKALTPPEEWPRHVMHEQRGVAPLQALCERLKVPQDFRQLA
HHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHH
IIACREHLNVHRLAELRDRTLHELLVRCDAFRRPERIAQLALVCEADKRGRLGSEEAAYP
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
QGEALKRLHAAALAINARDLAAEGLQGPQIGEALTKARIAAIAAARRIGA
CHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]