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The map label for this gene is bioH [H]

Identifier: 78045956

GI number: 78045956

Start: 453223

End: 454152

Strand: Reverse

Name: bioH [H]

Synonym: XCV0400

Alternate gene names: 78045956

Gene position: 454152-453223 (Counterclockwise)

Preceding gene: 78045957

Following gene: 78045955

Centisome position: 8.77

GC content: 68.06

Gene sequence:

>930_bases
ATGCGCGCGCGCAATGGGCACTGCTGGCTCCAAAGATCGTCGCACTGCGTGCAGCCGCCACGCATTGACTCCCAGGCGCT
GCACCCAACATCGGATCTGCGTGCCTCCAATGACCAGCGCGCATCCCACGATGCGTGCCGGCACGCCACAATCGGCGACA
ATGCGCGCATGCATATCGATGTCATCGGCCACGGGCCTGCGCTGGTTCTCCTGCACGGTTGGGCACTGCACGGCGGCGTG
TTCGCACCGCTGGTGGATCGCCTGGCGCCGCACTATCAATTGCATCTGGTCGACCTGCCCGGGCACGGCTTCAGTCGCGA
CGACAGCACGCCGCTGGCATTGCCCTATGTGGTTGCAGAAATTGCGGCCGCCACACCGCCGGCGGTCTGGCTCGGCTGGT
CGCTGGGCGGGCTGTTTGCGCTGCATGCGGCCGCCACCCTGCGGCAGGTGCGTGGGTTGGCGATGATTGCCGCCACGCCG
CGTTTCGTGCGTGGCAACGATTGGCCGAGTGCCGTCCAGCGCGAGGTGTTCGTGCAATTCGGGATCGAGTTGTCGCGCGA
CTACCGCGGCACGCTGGAGCGTTTTCTTGCGCTGGACACACTGGGCTCGGCGCATGCACGCAGCGAACTGCGCAGCCTGC
GCGAGACGCTCACCGCGCGTGGCGAACCGGCACCGGAGGCGTTGCAGCAAGGGCTCGCCCTGCTCGAACGCACCGATCTG
CGCCGCGCCGTGCCGCAACTCGCACGCCCCAGCCTGTGGATCGCCGGGCAACGCGATCGCCTGGTGCCTGCCGCCGGCAT
GCATGCCACCGCCGCGCTCAGCCCACACGCGCAGGCGCTCACCATTGCCGGCGGTGGTCACGCGCCGTTTCTGGGTCATG
CAGATCAGGTGAGCGAGGCGCTGCAACGCTTCGTTGCGTCCGTGCCATGA

Upstream 100 bases:

>100_bases
GGCGTCTGGGACGGCTTCGATCCCGACTGGCTGGCTGCAGCGATCCAGGCGCGGCGCGCACGGCGCCGACACTGGCCGGC
GCGCTGGCACTGGTGCGGCC

Downstream 100 bases:

>100_bases
GCCGATCGTCGATCATGCGACATCGCTGATCGAGGGACATCTCGCATGGATCTGGGAATCGCTGGCCGCTGGGCGTTGGT
CTGCGCCGCAAGCAAAGGGC

Product: biotin biosynthesis protein BioH

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MRARNGHCWLQRSSHCVQPPRIDSQALHPTSDLRASNDQRASHDACRHATIGDNARMHIDVIGHGPALVLLHGWALHGGV
FAPLVDRLAPHYQLHLVDLPGHGFSRDDSTPLALPYVVAEIAAATPPAVWLGWSLGGLFALHAAATLRQVRGLAMIAATP
RFVRGNDWPSAVQREVFVQFGIELSRDYRGTLERFLALDTLGSAHARSELRSLRETLTARGEPAPEALQQGLALLERTDL
RRAVPQLARPSLWIAGQRDRLVPAAGMHATAALSPHAQALTIAGGGHAPFLGHADQVSEALQRFVASVP

Sequences:

>Translated_309_residues
MRARNGHCWLQRSSHCVQPPRIDSQALHPTSDLRASNDQRASHDACRHATIGDNARMHIDVIGHGPALVLLHGWALHGGV
FAPLVDRLAPHYQLHLVDLPGHGFSRDDSTPLALPYVVAEIAAATPPAVWLGWSLGGLFALHAAATLRQVRGLAMIAATP
RFVRGNDWPSAVQREVFVQFGIELSRDYRGTLERFLALDTLGSAHARSELRSLRETLTARGEPAPEALQQGLALLERTDL
RRAVPQLARPSLWIAGQRDRLVPAAGMHATAALSPHAQALTIAGGGHAPFLGHADQVSEALQRFVASVP
>Mature_309_residues
MRARNGHCWLQRSSHCVQPPRIDSQALHPTSDLRASNDQRASHDACRHATIGDNARMHIDVIGHGPALVLLHGWALHGGV
FAPLVDRLAPHYQLHLVDLPGHGFSRDDSTPLALPYVVAEIAAATPPAVWLGWSLGGLFALHAAATLRQVRGLAMIAATP
RFVRGNDWPSAVQREVFVQFGIELSRDYRGTLERFLALDTLGSAHARSELRSLRETLTARGEPAPEALQQGLALLERTDL
RRAVPQLARPSLWIAGQRDRLVPAAGMHATAALSPHAQALTIAGGGHAPFLGHADQVSEALQRFVASVP

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Escherichia coli, GI1789817, Length=227, Percent_Identity=38.7665198237885, Blast_Score=150, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 33409; Mature: 33409

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRARNGHCWLQRSSHCVQPPRIDSQALHPTSDLRASNDQRASHDACRHATIGDNARMHID
CCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHCCCCCCCCEEEEE
VIGHGPALVLLHGWALHGGVFAPLVDRLAPHYQLHLVDLPGHGFSRDDSTPLALPYVVAE
EECCCCEEEEEECHHHHCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHH
IAAATPPAVWLGWSLGGLFALHAAATLRQVRGLAMIAATPRFVRGNDWPSAVQREVFVQF
HHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHH
GIELSRDYRGTLERFLALDTLGSAHARSELRSLRETLTARGEPAPEALQQGLALLERTDL
CCHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
RRAVPQLARPSLWIAGQRDRLVPAAGMHATAALSPHAQALTIAGGGHAPFLGHADQVSEA
HHHHHHHHCCCEEEECCCCCCCCCCCCCHHEECCCCCCEEEEECCCCCCCCCCHHHHHHH
LQRFVASVP
HHHHHHCCC
>Mature Secondary Structure
MRARNGHCWLQRSSHCVQPPRIDSQALHPTSDLRASNDQRASHDACRHATIGDNARMHID
CCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHCCCCCCCCEEEEE
VIGHGPALVLLHGWALHGGVFAPLVDRLAPHYQLHLVDLPGHGFSRDDSTPLALPYVVAE
EECCCCEEEEEECHHHHCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHH
IAAATPPAVWLGWSLGGLFALHAAATLRQVRGLAMIAATPRFVRGNDWPSAVQREVFVQF
HHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHH
GIELSRDYRGTLERFLALDTLGSAHARSELRSLRETLTARGEPAPEALQQGLALLERTDL
CCHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
RRAVPQLARPSLWIAGQRDRLVPAAGMHATAALSPHAQALTIAGGGHAPFLGHADQVSEA
HHHHHHHHCCCEEEECCCCCCCCCCCCCHHEECCCCCCEEEEECCCCCCCCCCHHHHHHH
LQRFVASVP
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]