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The map label for this gene is glpK [H]

Identifier: 78045928

GI number: 78045928

Start: 423874

End: 425373

Strand: Reverse

Name: glpK [H]

Synonym: XCV0372

Alternate gene names: 78045928

Gene position: 425373-423874 (Counterclockwise)

Preceding gene: 78045929

Following gene: 78045926

Centisome position: 8.21

GC content: 63.0

Gene sequence:

>1500_bases
ATGGAAAAGCAGTACGTCCTTGCGATCGATCAAGGCACCACCAGCTCACGCGCGATGTTGTTCGACCGCCAGGGCAAGGT
GGCCGGCGTGGCGCAGCGCGAATTCGGCCAGATCTTTCCGCAGCCGGGTTGGGTGGAACACAACCCTCGCGAGATCATGA
CCAGCGTCTATACCACCATCACCGAGCTGCTCAACAACGCGCAGATCGATGCGCGCGCGATCGCCGGGATCGGCATCACC
AACCAGCGCGAGACCGCGGTGGTGTGGGACAAGGCCACCGGCCAGCCGATCTACAACGCCATCGTGTGGCAATCGCGGCA
GACCAAGGACATCTGCACCCAGCTCAAGGAGGCCGGGCATGAGCAGATGGTGCGCGAGAAGACCGGCCTGCTGATCGATG
CGTATTTTTCCGGGACCAAGGTCAAGTGGATTCTCGACCACGTCGAAGGCGCGCGCGAACGCGCGCAGAAAGGCGAACTG
GCGTTCGGCACCATCGACAGTTGGCTGATCTGGAATCTCACCGGCGGCAAGGTGCACGTCACCGACTACACCAATGCCTC
GCGCACCATGATGTTCAACATCCACACGCTTGAGTGGGATGCTGAACTGCTGGCCATGTTGGACGTGCCCGCGCAGATGC
TGCCGGACGTGCGTTCGTCCAGCGAGATCTATGGCATGACCCAGACCCAGTACTTCTACGGCGAACAGGTGCCCATCGCC
GGCATTGCCGGCGACCAGCAGGCCGCGTTATTCGGCCAGGCCTGCTTCGAACCGGGCATGGCGAAAAACACCTATGGCAC
CGGCTGTTTCATGCTGATGAATACCGGCGACAAGGCGGTGGCGTCCAAGGCCGGTTTGCTGACCACGATTGCCTGGGGCA
TTGACGGCAAGGTCGAATACGCGCTGGAAGGCGCGATCTTCGTTGCCGGCTCGGTGGTGCAGTGGCTGCGCGATGGCTTG
CGCATGCTGGGCAAGGCCAGCGACTCGCAAGCCTATGCCGAGCGTGCCGGCGACAACGATGGCGTGTACATCGTGCCGGC
CTTCGTCGGCCTGGGCGCGCCGTACTGGCGCAGCGACATCCGCGGCGCGGTGTTCGGGCTGACGCGTGGTACCACCAAGG
AACACTTCGTGCGTGCAGCAGTCGAATCGATGGCCTACCAGACCCGCGACGTGCTCACCGCGATGCAAAGCGATTCCGGC
ATCGAGCTGAAAGAACTGCGTGCCGACGGCGGTGCCATCGCCAACGACTTCATGGCGCAGTTCCAGAGTGACATTCTCAA
TGTGCCGGTACTCCGCCCGGAAGTCGCGGAAACCACTGCGCTGGGTGCGGCGTATCTGGCAGGGCTTGCGACTGGGTTCT
GGGGCAGCCGCGAAGAAATCGCCAAACAGTGGGCCGTGGATCGCCGCTTCGAACCGGCCATGGCCGACGACAAGCGCCAA
GGGCTCTACGCCGGCTGGCAGCAAGCAGTGGAAGCGACGATGGGATTCCGCATCAGCTGA

Upstream 100 bases:

>100_bases
GAAGTTACCTGTGGCCAAACCCTGATGGCCACGCGGCGTCATCGCCTGCACCGACCTTTCTCGCCGTGCGCCAATGCCGG
CATTGTTCGAGGAATTTTCG

Downstream 100 bases:

>100_bases
CACTGGTGCCGCAACACGACAGCGCCCAGTGTGAGGTGGGCGCTGTGATGAGCTCATCTTCGAGCGTCGAGGACACACGC
AGGGCCACGCAACGCAGCCT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MEKQYVLAIDQGTTSSRAMLFDRQGKVAGVAQREFGQIFPQPGWVEHNPREIMTSVYTTITELLNNAQIDARAIAGIGIT
NQRETAVVWDKATGQPIYNAIVWQSRQTKDICTQLKEAGHEQMVREKTGLLIDAYFSGTKVKWILDHVEGARERAQKGEL
AFGTIDSWLIWNLTGGKVHVTDYTNASRTMMFNIHTLEWDAELLAMLDVPAQMLPDVRSSSEIYGMTQTQYFYGEQVPIA
GIAGDQQAALFGQACFEPGMAKNTYGTGCFMLMNTGDKAVASKAGLLTTIAWGIDGKVEYALEGAIFVAGSVVQWLRDGL
RMLGKASDSQAYAERAGDNDGVYIVPAFVGLGAPYWRSDIRGAVFGLTRGTTKEHFVRAAVESMAYQTRDVLTAMQSDSG
IELKELRADGGAIANDFMAQFQSDILNVPVLRPEVAETTALGAAYLAGLATGFWGSREEIAKQWAVDRRFEPAMADDKRQ
GLYAGWQQAVEATMGFRIS

Sequences:

>Translated_499_residues
MEKQYVLAIDQGTTSSRAMLFDRQGKVAGVAQREFGQIFPQPGWVEHNPREIMTSVYTTITELLNNAQIDARAIAGIGIT
NQRETAVVWDKATGQPIYNAIVWQSRQTKDICTQLKEAGHEQMVREKTGLLIDAYFSGTKVKWILDHVEGARERAQKGEL
AFGTIDSWLIWNLTGGKVHVTDYTNASRTMMFNIHTLEWDAELLAMLDVPAQMLPDVRSSSEIYGMTQTQYFYGEQVPIA
GIAGDQQAALFGQACFEPGMAKNTYGTGCFMLMNTGDKAVASKAGLLTTIAWGIDGKVEYALEGAIFVAGSVVQWLRDGL
RMLGKASDSQAYAERAGDNDGVYIVPAFVGLGAPYWRSDIRGAVFGLTRGTTKEHFVRAAVESMAYQTRDVLTAMQSDSG
IELKELRADGGAIANDFMAQFQSDILNVPVLRPEVAETTALGAAYLAGLATGFWGSREEIAKQWAVDRRFEPAMADDKRQ
GLYAGWQQAVEATMGFRIS
>Mature_499_residues
MEKQYVLAIDQGTTSSRAMLFDRQGKVAGVAQREFGQIFPQPGWVEHNPREIMTSVYTTITELLNNAQIDARAIAGIGIT
NQRETAVVWDKATGQPIYNAIVWQSRQTKDICTQLKEAGHEQMVREKTGLLIDAYFSGTKVKWILDHVEGARERAQKGEL
AFGTIDSWLIWNLTGGKVHVTDYTNASRTMMFNIHTLEWDAELLAMLDVPAQMLPDVRSSSEIYGMTQTQYFYGEQVPIA
GIAGDQQAALFGQACFEPGMAKNTYGTGCFMLMNTGDKAVASKAGLLTTIAWGIDGKVEYALEGAIFVAGSVVQWLRDGL
RMLGKASDSQAYAERAGDNDGVYIVPAFVGLGAPYWRSDIRGAVFGLTRGTTKEHFVRAAVESMAYQTRDVLTAMQSDSG
IELKELRADGGAIANDFMAQFQSDILNVPVLRPEVAETTALGAAYLAGLATGFWGSREEIAKQWAVDRRFEPAMADDKRQ
GLYAGWQQAVEATMGFRIS

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=502, Percent_Identity=46.2151394422311, Blast_Score=452, Evalue=1e-127,
Organism=Homo sapiens, GI4504007, Length=502, Percent_Identity=46.2151394422311, Blast_Score=452, Evalue=1e-127,
Organism=Homo sapiens, GI42794763, Length=506, Percent_Identity=45.8498023715415, Blast_Score=451, Evalue=1e-127,
Organism=Homo sapiens, GI41393575, Length=505, Percent_Identity=46.1386138613861, Blast_Score=447, Evalue=1e-125,
Organism=Homo sapiens, GI88196792, Length=509, Percent_Identity=31.6306483300589, Blast_Score=292, Evalue=5e-79,
Organism=Escherichia coli, GI1790361, Length=495, Percent_Identity=61.4141414141414, Blast_Score=657, Evalue=0.0,
Organism=Escherichia coli, GI1790005, Length=524, Percent_Identity=24.236641221374, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787789, Length=527, Percent_Identity=24.0986717267552, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1789168, Length=483, Percent_Identity=23.6024844720497, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1789987, Length=492, Percent_Identity=24.1869918699187, Blast_Score=76, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17535599, Length=500, Percent_Identity=40, Blast_Score=376, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI71986140, Length=528, Percent_Identity=25.1893939393939, Blast_Score=184, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI71986147, Length=268, Percent_Identity=30.2238805970149, Blast_Score=129, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6321755, Length=479, Percent_Identity=38.2045929018789, Blast_Score=303, Evalue=6e-83,
Organism=Drosophila melanogaster, GI24655555, Length=503, Percent_Identity=40.3578528827038, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24655560, Length=503, Percent_Identity=40.3578528827038, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24655570, Length=503, Percent_Identity=40.3578528827038, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24655574, Length=503, Percent_Identity=40.3578528827038, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24655565, Length=503, Percent_Identity=40.3578528827038, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI17864214, Length=510, Percent_Identity=41.7647058823529, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24654573, Length=462, Percent_Identity=41.5584415584416, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI19922058, Length=525, Percent_Identity=33.7142857142857, Blast_Score=291, Evalue=1e-78,
Organism=Drosophila melanogaster, GI24652871, Length=491, Percent_Identity=34.4195519348269, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24656367, Length=487, Percent_Identity=29.1581108829569, Blast_Score=221, Evalue=8e-58,
Organism=Drosophila melanogaster, GI21357867, Length=503, Percent_Identity=28.2306163021869, Blast_Score=218, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24656372, Length=326, Percent_Identity=30.9815950920245, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI45552909, Length=292, Percent_Identity=28.4246575342466, Blast_Score=126, Evalue=4e-29,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 54819; Mature: 54819

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKQYVLAIDQGTTSSRAMLFDRQGKVAGVAQREFGQIFPQPGWVEHNPREIMTSVYTTI
CCCCEEEEEECCCCCCCEEEEECCCCEECHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
TELLNNAQIDARAIAGIGITNQRETAVVWDKATGQPIYNAIVWQSRQTKDICTQLKEAGH
HHHHCCCCCCHHHEEECCCCCCCCEEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHCCH
EQMVREKTGLLIDAYFSGTKVKWILDHVEGARERAQKGELAFGTIDSWLIWNLTGGKVHV
HHHHHHHCCCEEEEEECCCEEEHHHHHHHHHHHHHHCCCEEEEECCCEEEEEECCCEEEE
TDYTNASRTMMFNIHTLEWDAELLAMLDVPAQMLPDVRSSSEIYGMTQTQYFYGEQVPIA
EECCCCCEEEEEEEEEEECCHHHHHHHCCCHHHCCCCCCCCCEEECEEEEEECCCCCCEE
GIAGDQQAALFGQACFEPGMAKNTYGTGCFMLMNTGDKAVASKAGLLTTIAWGIDGKVEY
EECCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHCCHHEEEEECCCCEEEE
ALEGAIFVAGSVVQWLRDGLRMLGKASDSQAYAERAGDNDGVYIVPAFVGLGAPYWRSDI
EECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEHHHHCCCHHHHHHC
RGAVFGLTRGTTKEHFVRAAVESMAYQTRDVLTAMQSDSGIELKELRADGGAIANDFMAQ
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHH
FQSDILNVPVLRPEVAETTALGAAYLAGLATGFWGSREEIAKQWAVDRRFEPAMADDKRQ
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
GLYAGWQQAVEATMGFRIS
CHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEKQYVLAIDQGTTSSRAMLFDRQGKVAGVAQREFGQIFPQPGWVEHNPREIMTSVYTTI
CCCCEEEEEECCCCCCCEEEEECCCCEECHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
TELLNNAQIDARAIAGIGITNQRETAVVWDKATGQPIYNAIVWQSRQTKDICTQLKEAGH
HHHHCCCCCCHHHEEECCCCCCCCEEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHCCH
EQMVREKTGLLIDAYFSGTKVKWILDHVEGARERAQKGELAFGTIDSWLIWNLTGGKVHV
HHHHHHHCCCEEEEEECCCEEEHHHHHHHHHHHHHHCCCEEEEECCCEEEEEECCCEEEE
TDYTNASRTMMFNIHTLEWDAELLAMLDVPAQMLPDVRSSSEIYGMTQTQYFYGEQVPIA
EECCCCCEEEEEEEEEEECCHHHHHHHCCCHHHCCCCCCCCCEEECEEEEEECCCCCCEE
GIAGDQQAALFGQACFEPGMAKNTYGTGCFMLMNTGDKAVASKAGLLTTIAWGIDGKVEY
EECCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHCCHHEEEEECCCCEEEE
ALEGAIFVAGSVVQWLRDGLRMLGKASDSQAYAERAGDNDGVYIVPAFVGLGAPYWRSDI
EECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEHHHHCCCHHHHHHC
RGAVFGLTRGTTKEHFVRAAVESMAYQTRDVLTAMQSDSGIELKELRADGGAIANDFMAQ
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHH
FQSDILNVPVLRPEVAETTALGAAYLAGLATGFWGSREEIAKQWAVDRRFEPAMADDKRQ
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
GLYAGWQQAVEATMGFRIS
CHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]