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The map label for this gene is glpF [H]

Identifier: 78045929

GI number: 78045929

Start: 425513

End: 426334

Strand: Reverse

Name: glpF [H]

Synonym: XCV0373

Alternate gene names: 78045929

Gene position: 426334-425513 (Counterclockwise)

Preceding gene: 78045930

Following gene: 78045928

Centisome position: 8.23

GC content: 64.36

Gene sequence:

>822_bases
ATGAGCCGGCAACTCGTAGGTGAGTTGATCTCCGAGGCGGTCGCGATGTTCATCATCATCGCGCTCGGCGATTCGGTGGC
GGCGATGTACATCCTGTACGACCCCAGCCCTTATCAAAACGCCTATTGGGGCGTGTGTATCGCCTGGGGCCTGGCGGTGA
CACTGGCGATCTACGTGACCGGCTCGGTGTCCGGGACGCACGCCAATCCGGCGGTCACGCTGGCGCTGGCACTGTTCCGC
GGTTTTTCCTGGAAGAAGGTATTGCCCTACTGGTTTGCGCAGGTGGTCGGCGCATTCCTGGGCGCGGCCATCGTCTATCA
GTTGTACGGGCCGGTGATCGATCACTTCAACCAGGTGCAGCAGCTCACCCGTGAAGCCGGCGGCGCAGCCGGGGTGTTCT
TCACCGCACCGGGGCTGGCGGTCACGCCGATGCATGCACTGGTCGACCAGATCATCATCACGGCGTTCCTGATCTTCGGC
ATCTTCGCCATCACCGAGCAGTTCAACGAGGCCGCACCCACTGCCAACTCCGGCGCGTTGATGATCGGCTTGCTGGTCGC
CACGCTGGGCGCGTCGATGGGCTATCTGGAGGCCTGGGCGATGAACCCGGCGCGCGACTTCGGCCCGCGCCTGTTAGCGT
ATTTCGCCGGCTGGGGCGAGGCCGCGTTGCCGGCCAAGGACAACTATTGGTGGATCCCGATCGTTGGCCCGTTGATCGGC
GGGCCGATCGGCGCGCTGGCCTATCAGTGGCTGATCCTGCCATTCCTGCCGGCGCGGGTGCGCCATCTGCAAGGCGGCAG
CAAACCGCTCGATCCACTGTGA

Upstream 100 bases:

>100_bases
CGTGGAGGGCACCCCAGATGAGCATCACCGACCACCGCTGCGCGCGAGCAAGCGCGCTGCACGCTGTTTTCTGCATCTTC
GACCTTGCGGGCATGCACGC

Downstream 100 bases:

>100_bases
TGGTGCTGCGCCAAGCCAACAATCACGCTGACGACGCGTGAAGTTACCTGTGGCCAAACCCTGATGGCCACGCGGCGTCA
TCGCCTGCACCGACCTTTCT

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: Aquaglyceroporin [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSRQLVGELISEAVAMFIIIALGDSVAAMYILYDPSPYQNAYWGVCIAWGLAVTLAIYVTGSVSGTHANPAVTLALALFR
GFSWKKVLPYWFAQVVGAFLGAAIVYQLYGPVIDHFNQVQQLTREAGGAAGVFFTAPGLAVTPMHALVDQIIITAFLIFG
IFAITEQFNEAAPTANSGALMIGLLVATLGASMGYLEAWAMNPARDFGPRLLAYFAGWGEAALPAKDNYWWIPIVGPLIG
GPIGALAYQWLILPFLPARVRHLQGGSKPLDPL

Sequences:

>Translated_273_residues
MSRQLVGELISEAVAMFIIIALGDSVAAMYILYDPSPYQNAYWGVCIAWGLAVTLAIYVTGSVSGTHANPAVTLALALFR
GFSWKKVLPYWFAQVVGAFLGAAIVYQLYGPVIDHFNQVQQLTREAGGAAGVFFTAPGLAVTPMHALVDQIIITAFLIFG
IFAITEQFNEAAPTANSGALMIGLLVATLGASMGYLEAWAMNPARDFGPRLLAYFAGWGEAALPAKDNYWWIPIVGPLIG
GPIGALAYQWLILPFLPARVRHLQGGSKPLDPL
>Mature_272_residues
SRQLVGELISEAVAMFIIIALGDSVAAMYILYDPSPYQNAYWGVCIAWGLAVTLAIYVTGSVSGTHANPAVTLALALFRG
FSWKKVLPYWFAQVVGAFLGAAIVYQLYGPVIDHFNQVQQLTREAGGAAGVFFTAPGLAVTPMHALVDQIIITAFLIFGI
FAITEQFNEAAPTANSGALMIGLLVATLGASMGYLEAWAMNPARDFGPRLLAYFAGWGEAALPAKDNYWWIPIVGPLIGG
PIGALAYQWLILPFLPARVRHLQGGSKPLDPL

Specific function: Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=249, Percent_Identity=38.5542168674699, Blast_Score=167, Evalue=7e-42,
Organism=Homo sapiens, GI157266307, Length=255, Percent_Identity=38.8235294117647, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI4502187, Length=265, Percent_Identity=32.8301886792453, Blast_Score=147, Evalue=7e-36,
Organism=Homo sapiens, GI22538420, Length=248, Percent_Identity=32.6612903225806, Blast_Score=142, Evalue=2e-34,
Organism=Homo sapiens, GI310133356, Length=180, Percent_Identity=32.2222222222222, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI310114181, Length=180, Percent_Identity=32.2222222222222, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI4502181, Length=261, Percent_Identity=30.2681992337165, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI4755125, Length=261, Percent_Identity=30.2681992337165, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI6912506, Length=228, Percent_Identity=30.7017543859649, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI4502183, Length=231, Percent_Identity=33.7662337662338, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI37694062, Length=267, Percent_Identity=28.8389513108614, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4502179, Length=259, Percent_Identity=29.3436293436293, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI86792455, Length=211, Percent_Identity=30.8056872037915, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI45446752, Length=169, Percent_Identity=36.094674556213, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1790362, Length=264, Percent_Identity=40.1515151515151, Blast_Score=172, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI71992966, Length=257, Percent_Identity=35.7976653696498, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17544068, Length=254, Percent_Identity=35.0393700787402, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17531429, Length=276, Percent_Identity=30.4347826086957, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17531431, Length=276, Percent_Identity=30.4347826086957, Blast_Score=120, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI71994009, Length=259, Percent_Identity=28.957528957529, Blast_Score=120, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI71992961, Length=253, Percent_Identity=32.0158102766798, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17533613, Length=247, Percent_Identity=28.3400809716599, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI32564052, Length=247, Percent_Identity=28.3400809716599, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17558372, Length=254, Percent_Identity=28.740157480315, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6321054, Length=183, Percent_Identity=39.344262295082, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6322985, Length=215, Percent_Identity=34.4186046511628, Blast_Score=105, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24762342, Length=248, Percent_Identity=31.0483870967742, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI45550503, Length=249, Percent_Identity=29.3172690763052, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 29248; Mature: 29116

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQLVGELISEAVAMFIIIALGDSVAAMYILYDPSPYQNAYWGVCIAWGLAVTLAIYVT
CCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHC
GSVSGTHANPAVTLALALFRGFSWKKVLPYWFAQVVGAFLGAAIVYQLYGPVIDHFNQVQ
CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLTREAGGAAGVFFTAPGLAVTPMHALVDQIIITAFLIFGIFAITEQFNEAAPTANSGAL
HHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
MIGLLVATLGASMGYLEAWAMNPARDFGPRLLAYFAGWGEAALPAKDNYWWIPIVGPLIG
HHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHC
GPIGALAYQWLILPFLPARVRHLQGGSKPLDPL
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SRQLVGELISEAVAMFIIIALGDSVAAMYILYDPSPYQNAYWGVCIAWGLAVTLAIYVT
CHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHC
GSVSGTHANPAVTLALALFRGFSWKKVLPYWFAQVVGAFLGAAIVYQLYGPVIDHFNQVQ
CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLTREAGGAAGVFFTAPGLAVTPMHALVDQIIITAFLIFGIFAITEQFNEAAPTANSGAL
HHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
MIGLLVATLGASMGYLEAWAMNPARDFGPRLLAYFAGWGEAALPAKDNYWWIPIVGPLIG
HHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHC
GPIGALAYQWLILPFLPARVRHLQGGSKPLDPL
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]