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The map label for this gene is ilvD

Identifier: 78045160

GI number: 78045160

Start: 446319

End: 447980

Strand: Direct

Name: ilvD

Synonym: CHY_0516

Alternate gene names: 78045160

Gene position: 446319-447980 (Clockwise)

Preceding gene: 78043021

Following gene: 78044561

Centisome position: 18.58

GC content: 48.8

Gene sequence:

>1662_bases
ATGCCAAGTCACCTCATTAAGTCAGGCATTAGCAAAGCACCGCATCGAAGTTTACTTAAGGCTTTGGGAATTACCGATAA
TGAGCTAAAACGCCCGTTTATCGGCGTTGTTCACAGTTTTTCGGAAATTGTTCCCGGGCACGTTCATTTAAGAACGGTGG
TTGAAGCGGTAAAAGCCGGAGTGAGAACTGCCGGCGGGGTTCCCTTTGAGCTCCCGGTAATCGGTGTTTGCGATGGTATA
GCCATGAACCACGAGGGAATGAAGTATTCGCTTGCCAGCCGGGAACTTATTGCCGATTCGGTGGAAGTAATGGTTAAAGC
TCACCAGTTTGATGCCCTTGTCTTTGTTCCCAACTGCGACAAAATTGTTCCCGGAATGTTAATTGCGGCAATGCGTTTAA
ATCTTCCGGCTATTTTTGTCAGCGGTGGGCCGATGTTAGCGGGAAGAGTAAGGGGAAAAAGCGTTTCCCTCAGCAATGTT
TTTGAAGGGGTAGGGGCTGTTGCTGCCGGCCTTATGAGTGAAGAGGAACTTTTAGAGTATGAAAATTACGCCTGCCCCGG
CTGTGGCTCGTGTGCCGGGATGTTTACCGCCAACTCGATGAACTGTTTAACCGAGGCCATAGGGATGGCCCTTCCCGGAA
ACGGCACTATTCCGGCGGTGTATGCCGAAAGGTTAAGGCTTGCTAAACTTTCCGGGGAAAAGGTAATGGAACTACTTTCC
CAAAATATCCGTCCCCGGGATATCTTTACCCGGGAGAGTTTGTTAAATGGTTTGGCGGTAGATATGGCTTTAGGGTGCTC
GACCAATACGGTTTTACATTTGGCGGCGGTAGCTTATGAGGCGGGAGTGGAGTTCGATTTAAACCTGGTTAACGAGGTAA
GTGAAAGAACGCCAAACCTCTGCCGTTTAAGCCCGGCGGGACCGCACCACATGGAAGACCTGTATTTTGCCGGGGGAATT
CCGGCGGTAATGAAGGAGCTTTCAAAGAAAGGTTTGATCAGAGAAGACCTCCTTACCGTTTCGGGAAAAACGGTGGGGGA
AAACCTGAAAAATGCTGTCAATAAAAATCCCGAAGTTATCCGACCCATTGATAATCCTTACTCGGAGACGGGGGGCTTAG
CAATTTTGTTTGGAACCCTGGCACCCCGGGGAGCGGTGGTGAAAAAATCGGCGGTTTTACCGGAAATGTTAACCCATACC
GGTCCGGCCCGGGTTTTTGATAGCGAAGAAGATGCTACCACTGCCATTTTAACCGGGCAAATAAAGAAAGGCGATGTGGT
GGTTATCCGCTATGAAGGCCCCAAAGGTGGTCCTGGCATGCGGGAGATGTTAACACCAACTTCGGCCCTTGCCGGGATGG
GCTTGGATAAAGATGTGGCATTGATTACCGATGGCCGGTTTTCGGGAGCAACCCGGGGAGCAGCAATCGGGCATGTTTCT
CCCGAAGGTTATGAAGGTGGACCGATAGCTTTGGTTCGGGAAGGAGACTTAATAAAAATAGATATTCCGGGGCAAAGATT
AGATCTTTTAGTATCAGAAGAAGAACTTAACCAAAGAAAAGCCCGGTTACAAATTCCCGAGCCTAAGATTAAAGAAGGTT
ACCTTGCTCGTTATCAAAAATTAGTGAGCTCTGCTAATTTAGGTGCGGTGTTTTTAAAGTAG

Upstream 100 bases:

>100_bases
AGGTGTACCCGGTCCTATGACCAAGACGTTAATCAAAGATTTCCGGGAATACGTTACTACCGACGGTCCATTTATTTTCA
GCGAGTAGAGGGGGAGAAAG

Downstream 100 bases:

>100_bases
GGGTGAGCGAAATGGAGTTAACCGGAGCGCAAATTTTAATTAAAGTATTAGAGGATTTAGGAGTGGATACTGTATTTGGC
TATCCCGGTGGAGCGGTACT

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MPSHLIKSGISKAPHRSLLKALGITDNELKRPFIGVVHSFSEIVPGHVHLRTVVEAVKAGVRTAGGVPFELPVIGVCDGI
AMNHEGMKYSLASRELIADSVEVMVKAHQFDALVFVPNCDKIVPGMLIAAMRLNLPAIFVSGGPMLAGRVRGKSVSLSNV
FEGVGAVAAGLMSEEELLEYENYACPGCGSCAGMFTANSMNCLTEAIGMALPGNGTIPAVYAERLRLAKLSGEKVMELLS
QNIRPRDIFTRESLLNGLAVDMALGCSTNTVLHLAAVAYEAGVEFDLNLVNEVSERTPNLCRLSPAGPHHMEDLYFAGGI
PAVMKELSKKGLIREDLLTVSGKTVGENLKNAVNKNPEVIRPIDNPYSETGGLAILFGTLAPRGAVVKKSAVLPEMLTHT
GPARVFDSEEDATTAILTGQIKKGDVVVIRYEGPKGGPGMREMLTPTSALAGMGLDKDVALITDGRFSGATRGAAIGHVS
PEGYEGGPIALVREGDLIKIDIPGQRLDLLVSEEELNQRKARLQIPEPKIKEGYLARYQKLVSSANLGAVFLK

Sequences:

>Translated_553_residues
MPSHLIKSGISKAPHRSLLKALGITDNELKRPFIGVVHSFSEIVPGHVHLRTVVEAVKAGVRTAGGVPFELPVIGVCDGI
AMNHEGMKYSLASRELIADSVEVMVKAHQFDALVFVPNCDKIVPGMLIAAMRLNLPAIFVSGGPMLAGRVRGKSVSLSNV
FEGVGAVAAGLMSEEELLEYENYACPGCGSCAGMFTANSMNCLTEAIGMALPGNGTIPAVYAERLRLAKLSGEKVMELLS
QNIRPRDIFTRESLLNGLAVDMALGCSTNTVLHLAAVAYEAGVEFDLNLVNEVSERTPNLCRLSPAGPHHMEDLYFAGGI
PAVMKELSKKGLIREDLLTVSGKTVGENLKNAVNKNPEVIRPIDNPYSETGGLAILFGTLAPRGAVVKKSAVLPEMLTHT
GPARVFDSEEDATTAILTGQIKKGDVVVIRYEGPKGGPGMREMLTPTSALAGMGLDKDVALITDGRFSGATRGAAIGHVS
PEGYEGGPIALVREGDLIKIDIPGQRLDLLVSEEELNQRKARLQIPEPKIKEGYLARYQKLVSSANLGAVFLK
>Mature_552_residues
PSHLIKSGISKAPHRSLLKALGITDNELKRPFIGVVHSFSEIVPGHVHLRTVVEAVKAGVRTAGGVPFELPVIGVCDGIA
MNHEGMKYSLASRELIADSVEVMVKAHQFDALVFVPNCDKIVPGMLIAAMRLNLPAIFVSGGPMLAGRVRGKSVSLSNVF
EGVGAVAAGLMSEEELLEYENYACPGCGSCAGMFTANSMNCLTEAIGMALPGNGTIPAVYAERLRLAKLSGEKVMELLSQ
NIRPRDIFTRESLLNGLAVDMALGCSTNTVLHLAAVAYEAGVEFDLNLVNEVSERTPNLCRLSPAGPHHMEDLYFAGGIP
AVMKELSKKGLIREDLLTVSGKTVGENLKNAVNKNPEVIRPIDNPYSETGGLAILFGTLAPRGAVVKKSAVLPEMLTHTG
PARVFDSEEDATTAILTGQIKKGDVVVIRYEGPKGGPGMREMLTPTSALAGMGLDKDVALITDGRFSGATRGAAIGHVSP
EGYEGGPIALVREGDLIKIDIPGQRLDLLVSEEELNQRKARLQIPEPKIKEGYLARYQKLVSSANLGAVFLK

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family

Homologues:

Organism=Escherichia coli, GI48994964, Length=597, Percent_Identity=44.8911222780569, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1788157, Length=523, Percent_Identity=32.887189292543, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI1786464, Length=530, Percent_Identity=34.3396226415094, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI2367371, Length=541, Percent_Identity=31.0536044362292, Blast_Score=213, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6322476, Length=559, Percent_Identity=40.9660107334526, Blast_Score=413, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVD_CARHZ (Q3AER0)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359374.1
- ProteinModelPortal:   Q3AER0
- SMR:   Q3AER0
- STRING:   Q3AER0
- GeneID:   3728286
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0516
- NMPDR:   fig|246194.3.peg.1083
- TIGR:   CHY_0516
- eggNOG:   COG0129
- HOGENOM:   HBG671001
- OMA:   QSERWES
- BioCyc:   CHYD246194:CHY_0516-MONOMER
- HAMAP:   MF_00012
- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558
- PANTHER:   PTHR21000
- TIGRFAMs:   TIGR00110

Pfam domain/function: PF00920 ILVD_EDD; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.9

Molecular weight: Translated: 58842; Mature: 58711

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00886 ILVD_EDD_1; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSHLIKSGISKAPHRSLLKALGITDNELKRPFIGVVHSFSEIVPGHVHLRTVVEAVKAG
CCCHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VRTAGGVPFELPVIGVCDGIAMNHEGMKYSLASRELIADSVEVMVKAHQFDALVFVPNCD
HHHCCCCCEECCEEEEECCEEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
KIVPGMLIAAMRLNLPAIFVSGGPMLAGRVRGKSVSLSNVFEGVGAVAAGLMSEEELLEY
HHHHHHHHHHHHCCCCEEEEECCCEEEEECCCCEECHHHHHHHHHHHHHHHCCHHHHHHH
ENYACPGCGSCAGMFTANSMNCLTEAIGMALPGNGTIPAVYAERLRLAKLSGEKVMELLS
HCCCCCCCCCCHHHHCCCHHHHHHHHHCEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHH
QNIRPRDIFTRESLLNGLAVDMALGCSTNTVLHLAAVAYEAGVEFDLNLVNEVSERTPNL
CCCCCCHHHHHHHHHCCHHHHHEECCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCE
CRLSPAGPHHMEDLYFAGGIPAVMKELSKKGLIREDLLTVSGKTVGENLKNAVNKNPEVI
EEECCCCCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHCCCCCEE
RPIDNPYSETGGLAILFGTLAPRGAVVKKSAVLPEMLTHTGPARVFDSEEDATTAILTGQ
CCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCEEEEEECC
IKKGDVVVIRYEGPKGGPGMREMLTPTSALAGMGLDKDVALITDGRFSGATRGAAIGHVS
CCCCCEEEEEECCCCCCCCHHHHHCCHHHHHCCCCCCCEEEEECCCCCCCCCCCEEECCC
PEGYEGGPIALVREGDLIKIDIPGQRLDLLVSEEELNQRKARLQIPEPKIKEGYLARYQK
CCCCCCCCEEEEECCCEEEEECCCCCEEEEECHHHHHHHHHEECCCCCCCHHHHHHHHHH
LVSSANLGAVFLK
HHHCCCCCEEEEC
>Mature Secondary Structure 
PSHLIKSGISKAPHRSLLKALGITDNELKRPFIGVVHSFSEIVPGHVHLRTVVEAVKAG
CCHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VRTAGGVPFELPVIGVCDGIAMNHEGMKYSLASRELIADSVEVMVKAHQFDALVFVPNCD
HHHCCCCCEECCEEEEECCEEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
KIVPGMLIAAMRLNLPAIFVSGGPMLAGRVRGKSVSLSNVFEGVGAVAAGLMSEEELLEY
HHHHHHHHHHHHCCCCEEEEECCCEEEEECCCCEECHHHHHHHHHHHHHHHCCHHHHHHH
ENYACPGCGSCAGMFTANSMNCLTEAIGMALPGNGTIPAVYAERLRLAKLSGEKVMELLS
HCCCCCCCCCCHHHHCCCHHHHHHHHHCEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHH
QNIRPRDIFTRESLLNGLAVDMALGCSTNTVLHLAAVAYEAGVEFDLNLVNEVSERTPNL
CCCCCCHHHHHHHHHCCHHHHHEECCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCE
CRLSPAGPHHMEDLYFAGGIPAVMKELSKKGLIREDLLTVSGKTVGENLKNAVNKNPEVI
EEECCCCCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHCCCCCEE
RPIDNPYSETGGLAILFGTLAPRGAVVKKSAVLPEMLTHTGPARVFDSEEDATTAILTGQ
CCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCEEEEEECC
IKKGDVVVIRYEGPKGGPGMREMLTPTSALAGMGLDKDVALITDGRFSGATRGAAIGHVS
CCCCCEEEEEECCCCCCCCHHHHHCCHHHHHCCCCCCCEEEEECCCCCCCCCCCEEECCC
PEGYEGGPIALVREGDLIKIDIPGQRLDLLVSEEELNQRKARLQIPEPKIKEGYLARYQK
CCCCCCCCEEEEECCCEEEEECCCCCEEEEECHHHHHHHHHEECCCCCCCHHHHHHHHHH
LVSSANLGAVFLK
HHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA