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The map label for this gene is ilvE [H]

Identifier: 78043021

GI number: 78043021

Start: 445425

End: 446306

Strand: Direct

Name: ilvE [H]

Synonym: CHY_0515

Alternate gene names: 78043021

Gene position: 445425-446306 (Clockwise)

Preceding gene: 78042636

Following gene: 78045160

Centisome position: 18.55

GC content: 44.44

Gene sequence:

>882_bases
ATGGGGCTTCTTATTTACCTGGATGGAAAGTTTGTACCGGAGGAGGAAGCGAAAGTTTCGGTATTTGACCACGGTCTTTT
ATATGGTGATGGGGTATTTGAAGGTATCAGAGCGTACAACAACCGGGTATTTAAGCTAAAAGAACATATCGACCGGCTTT
ACGAGTCGGCCAAGGCTATTCTTTTAGAAATACCTCTAACCAAGGAAGAAATGACCGAAGTTGTTCTGGAAACCATGCGT
AAAAACAACTTACGGGAAGGATATATTCGGTTGGTGGTTACCCGGGGGAAAGGTGACCTGGGCTTAGACCCCAGAAAATG
TCCAAAAGCTACGGTTTTTTGCATCGGTTCTTCCATTACCCTTTATCCCGAGCGTTTTTACGAGGAAGGCCTTGAAGTGA
TTACCGTGCCAACCCGGCGCAACTTAAACGAAGCTTCCAGCCCCCGAATTAAGTCGTTAAATTACTTAAACAACATCCTG
GCCAAAATTGAAGCCAATTTACACGGGGTCTTAGAAGCAATTCTTCTAAATCAGGAGGGTTATGTGGCGGAAGCTACCGG
GGACAATGTTTTTATCGTTAAAAACGGTCGCTTAATTACACCACCTTCTTATGCCGGAATTTTAGAAGGTATTACCCGTA
ATACGGTTATGGATTTGGCCAGAAAAAGAGGCATTCCCGTGGAAGAAAAGCTGTTTACCCGCTATGACATCTTTAATGCC
GATGAGTGTTTCTTGACCGGTACTGCGGCGGAAGTAATACCGGTAGTAAAAGTTGATGGAAGAACTATCGGTGAAGGTGT
ACCCGGTCCTATGACCAAGACGTTAATCAAAGATTTCCGGGAATACGTTACTACCGACGGTCCATTTATTTTCAGCGAGT
AG

Upstream 100 bases:

>100_bases
GCAAAACCCCCGTCCCTGGACGTTCAGGGGCGGGGGTTGTTTTTATATATTTTTGGCATAAAAATTTATAATTTTTAATA
AAATTGCGGAGGTGTGGGAA

Downstream 100 bases:

>100_bases
AGGGGGAGAAAGATGCCAAGTCACCTCATTAAGTCAGGCATTAGCAAAGCACCGCATCGAAGTTTACTTAAGGCTTTGGG
AATTACCGATAATGAGCTAA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MGLLIYLDGKFVPEEEAKVSVFDHGLLYGDGVFEGIRAYNNRVFKLKEHIDRLYESAKAILLEIPLTKEEMTEVVLETMR
KNNLREGYIRLVVTRGKGDLGLDPRKCPKATVFCIGSSITLYPERFYEEGLEVITVPTRRNLNEASSPRIKSLNYLNNIL
AKIEANLHGVLEAILLNQEGYVAEATGDNVFIVKNGRLITPPSYAGILEGITRNTVMDLARKRGIPVEEKLFTRYDIFNA
DECFLTGTAAEVIPVVKVDGRTIGEGVPGPMTKTLIKDFREYVTTDGPFIFSE

Sequences:

>Translated_293_residues
MGLLIYLDGKFVPEEEAKVSVFDHGLLYGDGVFEGIRAYNNRVFKLKEHIDRLYESAKAILLEIPLTKEEMTEVVLETMR
KNNLREGYIRLVVTRGKGDLGLDPRKCPKATVFCIGSSITLYPERFYEEGLEVITVPTRRNLNEASSPRIKSLNYLNNIL
AKIEANLHGVLEAILLNQEGYVAEATGDNVFIVKNGRLITPPSYAGILEGITRNTVMDLARKRGIPVEEKLFTRYDIFNA
DECFLTGTAAEVIPVVKVDGRTIGEGVPGPMTKTLIKDFREYVTTDGPFIFSE
>Mature_292_residues
GLLIYLDGKFVPEEEAKVSVFDHGLLYGDGVFEGIRAYNNRVFKLKEHIDRLYESAKAILLEIPLTKEEMTEVVLETMRK
NNLREGYIRLVVTRGKGDLGLDPRKCPKATVFCIGSSITLYPERFYEEGLEVITVPTRRNLNEASSPRIKSLNYLNNILA
KIEANLHGVLEAILLNQEGYVAEATGDNVFIVKNGRLITPPSYAGILEGITRNTVMDLARKRGIPVEEKLFTRYDIFNAD
ECFLTGTAAEVIPVVKVDGRTIGEGVPGPMTKTLIKDFREYVTTDGPFIFSE

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=286, Percent_Identity=37.4125874125874, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI1787338, Length=253, Percent_Identity=26.0869565217391, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 32840; Mature: 32709

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLLIYLDGKFVPEEEAKVSVFDHGLLYGDGVFEGIRAYNNRVFKLKEHIDRLYESAKAI
CEEEEEECCEECCCCCCEEEEEECCEEECCHHHHHHHHHCCCEEHHHHHHHHHHHHHCEE
LLEIPLTKEEMTEVVLETMRKNNLREGYIRLVVTRGKGDLGLDPRKCPKATVFCIGSSIT
EEECCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCCEE
LYPERFYEEGLEVITVPTRRNLNEASSPRIKSLNYLNNILAKIEANLHGVLEAILLNQEG
ECCHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
YVAEATGDNVFIVKNGRLITPPSYAGILEGITRNTVMDLARKRGIPVEEKLFTRYDIFNA
CEEEECCCEEEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
DECFLTGTAAEVIPVVKVDGRTIGEGVPGPMTKTLIKDFREYVTTDGPFIFSE
CCEEEECCHHHEEEEEEECCEECCCCCCCHHHHHHHHHHHHHHCCCCCEEECC
>Mature Secondary Structure 
GLLIYLDGKFVPEEEAKVSVFDHGLLYGDGVFEGIRAYNNRVFKLKEHIDRLYESAKAI
EEEEEECCEECCCCCCEEEEEECCEEECCHHHHHHHHHCCCEEHHHHHHHHHHHHHCEE
LLEIPLTKEEMTEVVLETMRKNNLREGYIRLVVTRGKGDLGLDPRKCPKATVFCIGSSIT
EEECCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCCEE
LYPERFYEEGLEVITVPTRRNLNEASSPRIKSLNYLNNILAKIEANLHGVLEAILLNQEG
ECCHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
YVAEATGDNVFIVKNGRLITPPSYAGILEGITRNTVMDLARKRGIPVEEKLFTRYDIFNA
CEEEECCCEEEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
DECFLTGTAAEVIPVVKVDGRTIGEGVPGPMTKTLIKDFREYVTTDGPFIFSE
CCEEEECCHHHEEEEEEECCEECCCCCCCHHHHHHHHHHHHHHCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]