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The map label for this gene is pncA [H]

Identifier: 78045028

GI number: 78045028

Start: 595114

End: 595689

Strand: Direct

Name: pncA [H]

Synonym: CHY_0679

Alternate gene names: 78045028

Gene position: 595114-595689 (Clockwise)

Preceding gene: 78043954

Following gene: 78042631

Centisome position: 24.78

GC content: 44.1

Gene sequence:

>576_bases
ATGAAAGGTGACAGACACTGTATAGTTATCATTGACATGTTAAACGACTTCATTGGACCCAATGCTCCTTTAAGATGCCC
CGATGGGGAAAAAATCGTTCCCAACTTGCAAAAGCTTGTAGAATTTGCCCACGAAAACGGTATTAACGTTGTTTTTGTGC
AGGAAGCACACCGGAAAAACGATGCTGACTTTAGAGTACGTCCGGTTCATGCTGTTAAAGGTACGTGGGGTTCTGACTTT
ATTCCGGAACTTCGTCCCGATGAAGAAAAAGGTGATTACATTGTGCAAAAGCGCCGTCACAGTGCTTTTGCTTACACTGA
CTTAGACCTCTACCTCAGAGAAGAAAAAATCGACACCGTAGTAGTTACCGGTGTATGGACCAACGTTTGCGTAAGAAGCA
CCGCTTCCGATGCTCTTTATCATGCTTACAACGTAATTGCCATATCCGACTGTTGCGCTTCTGCTAACGAAGAAATGCAT
CAAGCAGGCTTGAGAGACATGGCGTTGTTTGCCGAAGTTATGACTTTAGAAGAATACATGAAAGAATGGCCCAATCGGGA
AGGTGCCAAGAAGTAA

Upstream 100 bases:

>100_bases
CGTAAAGCGGCAAACGATTTCTAAATTAGAACGCTGGATTAAGTTTTAACCTGTAAAAAGGTTAAAAATATCATTACGAA
TTATCAAAGGAGGAGAAAAT

Downstream 100 bases:

>100_bases
CTGTCTTTTAAGGGAAAGGTCTAAATTGGGCCTTTCCCGTCTTTACATTCTAAGGAGGCTTTTTTAAATGCGCATTGTAG
TAGGGGTAACCGGGGCAAGT

Product: isochorismatase family protein

Products: nicotinate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MKGDRHCIVIIDMLNDFIGPNAPLRCPDGEKIVPNLQKLVEFAHENGINVVFVQEAHRKNDADFRVRPVHAVKGTWGSDF
IPELRPDEEKGDYIVQKRRHSAFAYTDLDLYLREEKIDTVVVTGVWTNVCVRSTASDALYHAYNVIAISDCCASANEEMH
QAGLRDMALFAEVMTLEEYMKEWPNREGAKK

Sequences:

>Translated_191_residues
MKGDRHCIVIIDMLNDFIGPNAPLRCPDGEKIVPNLQKLVEFAHENGINVVFVQEAHRKNDADFRVRPVHAVKGTWGSDF
IPELRPDEEKGDYIVQKRRHSAFAYTDLDLYLREEKIDTVVVTGVWTNVCVRSTASDALYHAYNVIAISDCCASANEEMH
QAGLRDMALFAEVMTLEEYMKEWPNREGAKK
>Mature_191_residues
MKGDRHCIVIIDMLNDFIGPNAPLRCPDGEKIVPNLQKLVEFAHENGINVVFVQEAHRKNDADFRVRPVHAVKGTWGSDF
IPELRPDEEKGDYIVQKRRHSAFAYTDLDLYLREEKIDTVVVTGVWTNVCVRSTASDALYHAYNVIAISDCCASANEEMH
QAGLRDMALFAEVMTLEEYMKEWPNREGAKK

Specific function: Unknown

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=193, Percent_Identity=26.9430051813472, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081970, Length=210, Percent_Identity=25.2380952380952, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI87081992, Length=147, Percent_Identity=30.6122448979592, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: 3.5.1.19

Molecular weight: Translated: 21752; Mature: 21752

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGDRHCIVIIDMLNDFIGPNAPLRCPDGEKIVPNLQKLVEFAHENGINVVFVQEAHRKN
CCCCCEEEEEHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCC
DADFRVRPVHAVKGTWGSDFIPELRPDEEKGDYIVQKRRHSAFAYTDLDLYLREEKIDTV
CCCEEEEEEHEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECHHEEEECCCCCEE
VVTGVWTNVCVRSTASDALYHAYNVIAISDCCASANEEMHQAGLRDMALFAEVMTLEEYM
EEEEHHHHHHHHCCHHHHHHHHHHHEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
KEWPNREGAKK
HHCCCCCCCCC
>Mature Secondary Structure
MKGDRHCIVIIDMLNDFIGPNAPLRCPDGEKIVPNLQKLVEFAHENGINVVFVQEAHRKN
CCCCCEEEEEHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCC
DADFRVRPVHAVKGTWGSDFIPELRPDEEKGDYIVQKRRHSAFAYTDLDLYLREEKIDTV
CCCEEEEEEHEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECHHEEEECCCCCEE
VVTGVWTNVCVRSTASDALYHAYNVIAISDCCASANEEMHQAGLRDMALFAEVMTLEEYM
EEEEHHHHHHHHCCHHHHHHHHHHHEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
KEWPNREGAKK
HHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nicotinamide; H2O

Specific reaction: nicotinamide + H2O = nicotinate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]