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The map label for this gene is allB [H]

Identifier: 78043954

GI number: 78043954

Start: 593647

End: 595062

Strand: Direct

Name: allB [H]

Synonym: CHY_0678

Alternate gene names: 78043954

Gene position: 593647-595062 (Clockwise)

Preceding gene: 78043349

Following gene: 78045028

Centisome position: 24.72

GC content: 46.4

Gene sequence:

>1416_bases
ATGGGTTTTGATTTGGTTTTAAAAAATGCCAAAATCCCTCAAGGCAACCGGACTGTGGACACCCACATCCTGGTGAAGGA
CGGTAAAATTGCCGGTTTTACCAATGACCTTACCGGGATTGAGGTTGCCGAAGTAATTGATGCTGAAAATAACCTTGTAT
TGCCTGGGTGCATTGACTCCCACACTCACTTTAATGATCCCGGCTTTACCCACCGGGAGAATTTTGCTACCGGTACTCAA
GCTGCTGCGGCTGGTGGGGTTACAACCATCATTGATATGCCCTGCTGTAGCATACCGTCGGTAAGGAGTGTGGATAACCT
TGAAGCAAAGTTAGAAGTAATCAAGGACAAAGCGTATGTAGACTATGCCATGTGGGGTGGGGCTACCGGCGAAGATGTGC
GGAACAATGCCCTCTACAACATTAAAGAACAAGCCGATTATGGAGTAGTAGCTTTTAAAGTTTACATGACTCCATCAGTT
CCAACTTATCCGCCGGCAAGCGATGCGGAGATGCTGGAAATTTTCCGGGAAGTAGCTAAGACCGGACTACCCGTTGGCAT
TCACGCGGAAAACTTGGACCTTTGCACTTATCACGTTAACAAATTAAGAAATGAAGGCCGTTTTGATGGGCCGGCCTGGG
CGGAAGCACGGTTGGAACTTGCGGAAAAAGTTGCTATTCAAATGGCCATTAGTTTTGCGGAAGAAACCGGTGCCCGGTTA
CATATCGTCCACATGAGCACCGGCATCGGTGGTGTGCTTGTAGGAGAAGCTAAGAAACGGGGTCTTGATGTAACCGCCGA
AACTTGTCCCCACTACCTGGTCTTAAATGCCTATGAATCAATGACCAAATGGCGTCAATTTGCCAAGATTGCACCGCCTT
TAAGAACTACCAGAGACAATGAAATGCTCTGGGAACAATTAGCTGATGGGCGGGTAGACTTTATTGCTACTGACCATGCA
CCTTATGAAATTGCTACCGAAAAAGAAGCTGAAGGCATGGACATCTGGACGGCGTTCCCGGGGATTCCGGGGGTAGAAAC
CATGGTACCGATTATTATCAGCGAAGGTTATAATAAAGGACGTCTTTCCTTATCCCGGTTAGTTGATGTTCTTTCCCGGA
ACGCAGCAATTCATTACGGTCTGTATCCGAAGAAAGGTGCCCTGGAAATTGGCTCCGATGCAGACTTTACCGTAATTGAC
CTTGACTATGAATGGACCATTGATAAAGATAAGATGTACACCATGGCCAAGTACACGCCGTTTCATGGTATGAAGCTTAA
AGGCAAACCTGTTAAGACCATCGTTCGCGGTAAACTCGTTTATGACGATAAGAACGGTATTGTTGGTAAGCCAGGATACG
GTCAATTCGTAAAGCGGCAAACGATTTCTAAATTAGAACGCTGGATTAAGTTTTAA

Upstream 100 bases:

>100_bases
GAACTGGTAGCCCTCTGGATTAATACTCCGCCTACCTTCTAAAAGGGTTATAAGCCCGAAAGGGCTCTAAATACCTAAAA
AACTTAAAGGGGGTTTTAAA

Downstream 100 bases:

>100_bases
CCTGTAAAAAGGTTAAAAATATCATTACGAATTATCAAAGGAGGAGAAAATATGAAAGGTGACAGACACTGTATAGTTAT
CATTGACATGTTAAACGACT

Product: allantoinase

Products: allantoate

Alternate protein names: Non-ATP-dependent L-selective hydantoinase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MGFDLVLKNAKIPQGNRTVDTHILVKDGKIAGFTNDLTGIEVAEVIDAENNLVLPGCIDSHTHFNDPGFTHRENFATGTQ
AAAAGGVTTIIDMPCCSIPSVRSVDNLEAKLEVIKDKAYVDYAMWGGATGEDVRNNALYNIKEQADYGVVAFKVYMTPSV
PTYPPASDAEMLEIFREVAKTGLPVGIHAENLDLCTYHVNKLRNEGRFDGPAWAEARLELAEKVAIQMAISFAEETGARL
HIVHMSTGIGGVLVGEAKKRGLDVTAETCPHYLVLNAYESMTKWRQFAKIAPPLRTTRDNEMLWEQLADGRVDFIATDHA
PYEIATEKEAEGMDIWTAFPGIPGVETMVPIIISEGYNKGRLSLSRLVDVLSRNAAIHYGLYPKKGALEIGSDADFTVID
LDYEWTIDKDKMYTMAKYTPFHGMKLKGKPVKTIVRGKLVYDDKNGIVGKPGYGQFVKRQTISKLERWIKF

Sequences:

>Translated_471_residues
MGFDLVLKNAKIPQGNRTVDTHILVKDGKIAGFTNDLTGIEVAEVIDAENNLVLPGCIDSHTHFNDPGFTHRENFATGTQ
AAAAGGVTTIIDMPCCSIPSVRSVDNLEAKLEVIKDKAYVDYAMWGGATGEDVRNNALYNIKEQADYGVVAFKVYMTPSV
PTYPPASDAEMLEIFREVAKTGLPVGIHAENLDLCTYHVNKLRNEGRFDGPAWAEARLELAEKVAIQMAISFAEETGARL
HIVHMSTGIGGVLVGEAKKRGLDVTAETCPHYLVLNAYESMTKWRQFAKIAPPLRTTRDNEMLWEQLADGRVDFIATDHA
PYEIATEKEAEGMDIWTAFPGIPGVETMVPIIISEGYNKGRLSLSRLVDVLSRNAAIHYGLYPKKGALEIGSDADFTVID
LDYEWTIDKDKMYTMAKYTPFHGMKLKGKPVKTIVRGKLVYDDKNGIVGKPGYGQFVKRQTISKLERWIKF
>Mature_470_residues
GFDLVLKNAKIPQGNRTVDTHILVKDGKIAGFTNDLTGIEVAEVIDAENNLVLPGCIDSHTHFNDPGFTHRENFATGTQA
AAAGGVTTIIDMPCCSIPSVRSVDNLEAKLEVIKDKAYVDYAMWGGATGEDVRNNALYNIKEQADYGVVAFKVYMTPSVP
TYPPASDAEMLEIFREVAKTGLPVGIHAENLDLCTYHVNKLRNEGRFDGPAWAEARLELAEKVAIQMAISFAEETGARLH
IVHMSTGIGGVLVGEAKKRGLDVTAETCPHYLVLNAYESMTKWRQFAKIAPPLRTTRDNEMLWEQLADGRVDFIATDHAP
YEIATEKEAEGMDIWTAFPGIPGVETMVPIIISEGYNKGRLSLSRLVDVLSRNAAIHYGLYPKKGALEIGSDADFTVIDL
DYEWTIDKDKMYTMAKYTPFHGMKLKGKPVKTIVRGKLVYDDKNGIVGKPGYGQFVKRQTISKLERWIKF

Specific function: Rather more predominant for the cleavage of aryl- than for alkyl-hydantoin derivatives. The stereoselectivity of this enzyme depends on the substrate used for bioconversion:strictly L-selective for the cleavage of D,L-5-indolylmethylhydantoin, but D-selec

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503379, Length=468, Percent_Identity=30.1282051282051, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI18105007, Length=416, Percent_Identity=29.5673076923077, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI4503375, Length=475, Percent_Identity=30.1052631578947, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI4503051, Length=467, Percent_Identity=29.1220556745182, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI62422571, Length=469, Percent_Identity=28.997867803838, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI4503377, Length=471, Percent_Identity=28.4501061571125, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI19923821, Length=465, Percent_Identity=29.4623655913978, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI190194363, Length=471, Percent_Identity=26.3269639065817, Blast_Score=138, Evalue=1e-32,
Organism=Escherichia coli, GI1786722, Length=465, Percent_Identity=31.8279569892473, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI87082175, Length=459, Percent_Identity=30.9368191721133, Blast_Score=208, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI193204318, Length=408, Percent_Identity=30.8823529411765, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI71989490, Length=474, Percent_Identity=28.9029535864979, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17539558, Length=471, Percent_Identity=27.8131634819533, Blast_Score=151, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI86575075, Length=460, Percent_Identity=24.3478260869565, Blast_Score=108, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6322218, Length=432, Percent_Identity=30.3240740740741, Blast_Score=199, Evalue=6e-52,
Organism=Drosophila melanogaster, GI24642586, Length=407, Percent_Identity=29.7297297297297, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI18859883, Length=454, Percent_Identity=30.8370044052863, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI17137462, Length=462, Percent_Identity=28.1385281385281, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI221377917, Length=474, Percent_Identity=27.8481012658228, Blast_Score=152, Evalue=6e-37,
Organism=Drosophila melanogaster, GI24644287, Length=324, Percent_Identity=26.8518518518519, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24644289, Length=239, Percent_Identity=28.0334728033473, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.5.2.5

Molecular weight: Translated: 52096; Mature: 51965

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFDLVLKNAKIPQGNRTVDTHILVKDGKIAGFTNDLTGIEVAEVIDAENNLVLPGCIDS
CCCEEEEECCCCCCCCCEEEEEEEEECCEEECCCCCCCCCCHHHHHCCCCCEEECCCCCC
HTHFNDPGFTHRENFATGTQAAAAGGVTTIIDMPCCSIPSVRSVDNLEAKLEVIKDKAYV
CCCCCCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCEE
DYAMWGGATGEDVRNNALYNIKEQADYGVVAFKVYMTPSVPTYPPASDAEMLEIFREVAK
EEEECCCCCCCHHCCCCEEEEHHCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
TGLPVGIHAENLDLCTYHVNKLRNEGRFDGPAWAEARLELAEKVAIQMAISFAEETGARL
CCCCEEEECCCCEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
HIVHMSTGIGGVLVGEAKKRGLDVTAETCPHYLVLNAYESMTKWRQFAKIAPPLRTTRDN
EEEEECCCCCEEEEECHHHCCCCEEHHHCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCH
EMLWEQLADGRVDFIATDHAPYEIATEKEAEGMDIWTAFPGIPGVETMVPIIISEGYNKG
HHHHHHHCCCCEEEEEECCCCEEEECCCCCCCCEEEEECCCCCCHHHHEEEEEECCCCCC
RLSLSRLVDVLSRNAAIHYGLYPKKGALEIGSDADFTVIDLDYEWTIDKDKMYTMAKYTP
CHHHHHHHHHHHCCCEEEECCCCCCCCEEECCCCCEEEEEEEEEEEECCCCEEEEEEECC
FHGMKLKGKPVKTIVRGKLVYDDKNGIVGKPGYGQFVKRQTISKLERWIKF
CCCEEECCCCHHHEEECEEEEECCCCEECCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GFDLVLKNAKIPQGNRTVDTHILVKDGKIAGFTNDLTGIEVAEVIDAENNLVLPGCIDS
CCEEEEECCCCCCCCCEEEEEEEEECCEEECCCCCCCCCCHHHHHCCCCCEEECCCCCC
HTHFNDPGFTHRENFATGTQAAAAGGVTTIIDMPCCSIPSVRSVDNLEAKLEVIKDKAYV
CCCCCCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCEE
DYAMWGGATGEDVRNNALYNIKEQADYGVVAFKVYMTPSVPTYPPASDAEMLEIFREVAK
EEEECCCCCCCHHCCCCEEEEHHCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
TGLPVGIHAENLDLCTYHVNKLRNEGRFDGPAWAEARLELAEKVAIQMAISFAEETGARL
CCCCEEEECCCCEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
HIVHMSTGIGGVLVGEAKKRGLDVTAETCPHYLVLNAYESMTKWRQFAKIAPPLRTTRDN
EEEEECCCCCEEEEECHHHCCCCEEHHHCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCH
EMLWEQLADGRVDFIATDHAPYEIATEKEAEGMDIWTAFPGIPGVETMVPIIISEGYNKG
HHHHHHHCCCCEEEEEECCCCEEEECCCCCCCCEEEEECCCCCCHHHHEEEEEECCCCCC
RLSLSRLVDVLSRNAAIHYGLYPKKGALEIGSDADFTVIDLDYEWTIDKDKMYTMAKYTP
CHHHHHHHHHHHCCCEEEECCCCCCCCEEECCCCCEEEEEEEEEEEECCCCEEEEEEECC
FHGMKLKGKPVKTIVRGKLVYDDKNGIVGKPGYGQFVKRQTISKLERWIKF
CCCEEECCCCHHHEEECEEEEECCCCEECCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-allantoin; H2O

Specific reaction: (S)-allantoin + H2O = allantoate

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: Dithiothreitol; p-Chloromercuribenzene-sulphonic acid [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9687026; 9650283 [H]