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The map label for this gene is glnA2 [H]

Identifier: 78044873

GI number: 78044873

Start: 628983

End: 630314

Strand: Direct

Name: glnA2 [H]

Synonym: CHY_0712

Alternate gene names: 78044873

Gene position: 628983-630314 (Clockwise)

Preceding gene: 78044110

Following gene: 78044229

Centisome position: 26.19

GC content: 44.74

Gene sequence:

>1332_bases
ATGAGTCCTACCAAAGAAGATATTTTAAAAATAGTTGAGGAAAAAGGAATACGCTTTATAAGGTTGCAATTTACCGATAT
TTACGGGATTCCCAAAAATGTGGCCATAACTGCGGAGCAGTTAGAGAAAGCTTTGGATGGGGAGTTAATGTTTGATGGCT
CCTCGATTGATGGTTTTGTTCGCATTGAAGAGTCGGATATGTACTTAAAGCCCGACCCTTCCACTTTTATGATTTTGCCC
TGGGCGGACCAGCGGTATGCGGAAGCGAGATTAATTTGCGATATTTATACCTATGATGGAAAGCCTTTTTCCGGCTGTCC
CCGGGGAAATTTAAAAAGAATCCTCCAGGAAGCAAAACAAATGGGGATTGATGAAATGTACGTGGGGCCGGAAGCGGAAT
TTTTCCTCTTTCAAACCAAAGAAGACGGTACGCTTACTACTGTTACCCATGATGAAGCCGGTTATTTTGACCTGGCTCCG
GTAGACCGGGGAGAAAATGCCCGGCGGGATATGGTACTGGCTTTGGAACAAATGGGCTTTGAAGTGGAAGCTTCCCACCA
TGAAGTGGCTCCCGGTCAGCACGAGATCGACTTTAAATACGACTCGGCGGTGCGAACTGCCGATAAAGTTATTACCTTCA
AGACGGTGGTACGGAAGATTGCCAAAAAACACGGGTTACATGCTACTTTTATGCCAAAGCCCGTTTACGGCATTGCCGGT
TCGGGAATGCATTTAAATATTTCCCTGGCGGCTAAAGGACAAAATATCTTTTACGATCCCAGTACCCCCCATCAGTTAAG
CGATATAGCATTGTATTTTATCGGTGGCTTATTAAAGCATGCCCGGGCAATTTCGGCCATAACCAATCCTCTGGTAAATT
CATATAAACGACTGGTTTCCGGATTTGAAGCACCGGTCTATATTGCCTGGTCGTTTAGAAACAGAAGTCCTTTAATACGG
ATTCCGGCTAAAAGAGGACCCTCTACCCGGATTGAGTTGAGAAGTCCTGATCCCTCGGCCAATCCTTACCTGGCATTTGC
GGTTTGCTTAAAAGCAGGTTTGGACGGCATTAAAAATAAAATTATGCCACCTCCTCCTTGCGACCGGAATATTTATCATA
TGGATGAAGAAGAAAGAAAAGCTTGGGGCATTGGTAATCTACCCGCCAGTTTGGGCGAAGCCTTAAAGGAGTTGGAGCAG
GATGAAGTAATCCGCGAAGCTTTGGGGGAACATATTTTTGAGCGGTATATGGAAGGTAAGCGCCTGGAATGGGATACTTA
CCGCATGCAGGTTCATGAATGGGAATTAAAGGAATATTTAGTGAAGTATTAA

Upstream 100 bases:

>100_bases
TTTTTGGCGGTTGTATGGATACTGGATACATAAGGAGTGAATAAAAAATTTTGGCAAAAATATGTTATAATAAGTGCAAA
TTGGGGTAAGGAGGTTAAAA

Downstream 100 bases:

>100_bases
AGCGGGCTAAGCCCGTTTTTTCTTAATTTTAAAAGTCTAAAATTTCACTTATCTCAAAAAATAAAAATTTTTGGGTAAAT
TTGTGATATAATTTATTATA

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MSPTKEDILKIVEEKGIRFIRLQFTDIYGIPKNVAITAEQLEKALDGELMFDGSSIDGFVRIEESDMYLKPDPSTFMILP
WADQRYAEARLICDIYTYDGKPFSGCPRGNLKRILQEAKQMGIDEMYVGPEAEFFLFQTKEDGTLTTVTHDEAGYFDLAP
VDRGENARRDMVLALEQMGFEVEASHHEVAPGQHEIDFKYDSAVRTADKVITFKTVVRKIAKKHGLHATFMPKPVYGIAG
SGMHLNISLAAKGQNIFYDPSTPHQLSDIALYFIGGLLKHARAISAITNPLVNSYKRLVSGFEAPVYIAWSFRNRSPLIR
IPAKRGPSTRIELRSPDPSANPYLAFAVCLKAGLDGIKNKIMPPPPCDRNIYHMDEEERKAWGIGNLPASLGEALKELEQ
DEVIREALGEHIFERYMEGKRLEWDTYRMQVHEWELKEYLVKY

Sequences:

>Translated_443_residues
MSPTKEDILKIVEEKGIRFIRLQFTDIYGIPKNVAITAEQLEKALDGELMFDGSSIDGFVRIEESDMYLKPDPSTFMILP
WADQRYAEARLICDIYTYDGKPFSGCPRGNLKRILQEAKQMGIDEMYVGPEAEFFLFQTKEDGTLTTVTHDEAGYFDLAP
VDRGENARRDMVLALEQMGFEVEASHHEVAPGQHEIDFKYDSAVRTADKVITFKTVVRKIAKKHGLHATFMPKPVYGIAG
SGMHLNISLAAKGQNIFYDPSTPHQLSDIALYFIGGLLKHARAISAITNPLVNSYKRLVSGFEAPVYIAWSFRNRSPLIR
IPAKRGPSTRIELRSPDPSANPYLAFAVCLKAGLDGIKNKIMPPPPCDRNIYHMDEEERKAWGIGNLPASLGEALKELEQ
DEVIREALGEHIFERYMEGKRLEWDTYRMQVHEWELKEYLVKY
>Mature_442_residues
SPTKEDILKIVEEKGIRFIRLQFTDIYGIPKNVAITAEQLEKALDGELMFDGSSIDGFVRIEESDMYLKPDPSTFMILPW
ADQRYAEARLICDIYTYDGKPFSGCPRGNLKRILQEAKQMGIDEMYVGPEAEFFLFQTKEDGTLTTVTHDEAGYFDLAPV
DRGENARRDMVLALEQMGFEVEASHHEVAPGQHEIDFKYDSAVRTADKVITFKTVVRKIAKKHGLHATFMPKPVYGIAGS
GMHLNISLAAKGQNIFYDPSTPHQLSDIALYFIGGLLKHARAISAITNPLVNSYKRLVSGFEAPVYIAWSFRNRSPLIRI
PAKRGPSTRIELRSPDPSANPYLAFAVCLKAGLDGIKNKIMPPPPCDRNIYHMDEEERKAWGIGNLPASLGEALKELEQD
EVIREALGEHIFERYMEGKRLEWDTYRMQVHEWELKEYLVKY

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=460, Percent_Identity=26.9565217391304, Blast_Score=159, Evalue=6e-39,
Organism=Escherichia coli, GI1790301, Length=474, Percent_Identity=41.9831223628692, Blast_Score=315, Evalue=5e-87,
Organism=Escherichia coli, GI87081870, Length=370, Percent_Identity=27.8378378378378, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 50301; Mature: 50170

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTKEDILKIVEEKGIRFIRLQFTDIYGIPKNVAITAEQLEKALDGELMFDGSSIDGFV
CCCCHHHHHHHHHHCCCEEEEEEEEEECCCCCCEEEEHHHHHHHHCCCEEEECCCCCEEE
RIEESDMYLKPDPSTFMILPWADQRYAEARLICDIYTYDGKPFSGCPRGNLKRILQEAKQ
EEECCCEEECCCCCEEEEEECCCCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHH
MGIDEMYVGPEAEFFLFQTKEDGTLTTVTHDEAGYFDLAPVDRGENARRDMVLALEQMGF
CCCCCEEECCCCEEEEEEECCCCEEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHCCC
EVEASHHEVAPGQHEIDFKYDSAVRTADKVITFKTVVRKIAKKHGLHATFMPKPVYGIAG
EEECCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEECC
SGMHLNISLAAKGQNIFYDPSTPHQLSDIALYFIGGLLKHARAISAITNPLVNSYKRLVS
CCEEEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GFEAPVYIAWSFRNRSPLIRIPAKRGPSTRIELRSPDPSANPYLAFAVCLKAGLDGIKNK
CCCCCEEEEEEECCCCCEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHC
IMPPPPCDRNIYHMDEEERKAWGIGNLPASLGEALKELEQDEVIREALGEHIFERYMEGK
CCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RLEWDTYRMQVHEWELKEYLVKY
CCEEHHEEEEEHHHHHHHHHHCC
>Mature Secondary Structure 
SPTKEDILKIVEEKGIRFIRLQFTDIYGIPKNVAITAEQLEKALDGELMFDGSSIDGFV
CCCHHHHHHHHHHCCCEEEEEEEEEECCCCCCEEEEHHHHHHHHCCCEEEECCCCCEEE
RIEESDMYLKPDPSTFMILPWADQRYAEARLICDIYTYDGKPFSGCPRGNLKRILQEAKQ
EEECCCEEECCCCCEEEEEECCCCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHH
MGIDEMYVGPEAEFFLFQTKEDGTLTTVTHDEAGYFDLAPVDRGENARRDMVLALEQMGF
CCCCCEEECCCCEEEEEEECCCCEEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHCCC
EVEASHHEVAPGQHEIDFKYDSAVRTADKVITFKTVVRKIAKKHGLHATFMPKPVYGIAG
EEECCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEECC
SGMHLNISLAAKGQNIFYDPSTPHQLSDIALYFIGGLLKHARAISAITNPLVNSYKRLVS
CCEEEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GFEAPVYIAWSFRNRSPLIRIPAKRGPSTRIELRSPDPSANPYLAFAVCLKAGLDGIKNK
CCCCCEEEEEEECCCCCEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHC
IMPPPPCDRNIYHMDEEERKAWGIGNLPASLGEALKELEQDEVIREALGEHIFERYMEGK
CCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RLEWDTYRMQVHEWELKEYLVKY
CCEEHHEEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2891680; 1981087 [H]