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The map label for this gene is lpdA [H]

Identifier: 78044229

GI number: 78044229

Start: 630453

End: 631823

Strand: Direct

Name: lpdA [H]

Synonym: CHY_0713

Alternate gene names: 78044229

Gene position: 630453-631823 (Clockwise)

Preceding gene: 78044873

Following gene: 78044809

Centisome position: 26.25

GC content: 43.4

Gene sequence:

>1371_bases
ATGTACGACGTAGCAATTTTAGGTGGCGGCCCCGGTGGTTATGTGGCAGCAATTAGAGCAGCTCAACTGGGGTTATCGGT
AGTAGTAGTGGAAAAAGATGAACTGGGTGGTACCTGTTTAAATCGCGGCTGTATTCCTACCAAAGCTCTTGTCTCTACAG
CAGAACGGCTTCATCAAATAAAAAATTCCGCCGCTTTTGGGATTGAGGTTACCGGTTACAATTTTAATTTTCAAAAAGCT
GCCGAACGGAAAAACCAGGTGGTAGAACGCTTGGTTAAAGGAATTCACTATCTTTTTAAGAAGAGCAAAATTACCTTGAT
TAAAGGAACCGGCAAATTAACCGGTAAAAATGAGATAACGGTCGAAACTTCTGATGGCCTGGAAAAGGTTGAAGCTAAAA
ATATAATCTTAGCCACTGGCTCAAAACCGGCATTAATTTCAGCTCTTGGCTACGATGGGGAAAGGGTTATTACCAGTGAT
GAAGCTTTAAACCTGGAAAAACTTCCCGCGGAAATGGTCATCATTGGTGGTGGGGTAATTGGGTCGGAATTTGCCACTAT
TTTTTCCGAAATGGGTGTTAAAGTGACCATTGTGGAACTTTTGCCATCAATCTTGGCAAATACCGATAAAGAGGTAAGCC
GTTATTTAACCACTTTATTTAAGAAACGGGGAATTCAGGTTAAAACCAAAGTAGCGGTAAAAGAAGTTAAAAAAGGTGAA
AAAGTTACGGTGGTTTTAGAAAACGGCGAGGAACTTGTTACCGACATGGTCTTAATTTCCATTGGCCGCGTACTAAATAC
CAAAGACATTGGTCTGGAGGAAGTGGGAGTAGCTCTGGGTCCCAAGGGAGAAGTTTTAGTGGATGAATATTTAAGAACAA
ATGTTGAAAACATCTATGCCATTGGCGATATAACCAGTAAAATGCAGCTGGCTCATGTGGCTTCGGCTCAAGGGATCCGG
GTGGTGGAAAATTTAGTGGGTGAACCCCAACCAATGAGTTACGATGTAGTCCCCGGTTGTATTTTTACTTTACCGGAAAT
AGCCATGGTGGGTTTGACCAGCCAGGAAGCGGAAGAAAAAGGAATTAAAATTATTACCGGTAAATTTCCCTTCCAGGCTT
CCGGGAAAGCGGTGGCGATGGAAGAAACGGAAGGTTTTGTGAAAATTATCGCTGACTTTTATACTCACCGGATCTTAGGC
GGACATATAGTTGGACCTCATGCAACGGATCTAATTGGCGAGATAGCTTTGGCGGTTCAGAAAGGACTGACCCTGGAGGA
AGTTGCCCATACCATTCATGCCCATCCGAGCTTACCTGAAGCGGTGATGGAAGCGGCGGAAGCGGCTTTAAATAGAGCTA
TTCACATTTAA

Upstream 100 bases:

>100_bases
AAAATTTCACTTATCTCAAAAAATAAAAATTTTTGGGTAAATTTGTGATATAATTTATTATATTAGAGCACAATTAACAA
ACATTGTTGGGGGGTTTAGA

Downstream 100 bases:

>100_bases
AGCAGGACGGGAGGGCTTTTATGAAAAAAATTACAGTCTTTTGGGGTTGCCAAATCCCCGGTCGCCTGACTTTTCTGGAA
AAAAGTACCCGTTTGGGACT

Product: alpha keto acid dehydrogenase complex, E3 component, lipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MYDVAILGGGPGGYVAAIRAAQLGLSVVVVEKDELGGTCLNRGCIPTKALVSTAERLHQIKNSAAFGIEVTGYNFNFQKA
AERKNQVVERLVKGIHYLFKKSKITLIKGTGKLTGKNEITVETSDGLEKVEAKNIILATGSKPALISALGYDGERVITSD
EALNLEKLPAEMVIIGGGVIGSEFATIFSEMGVKVTIVELLPSILANTDKEVSRYLTTLFKKRGIQVKTKVAVKEVKKGE
KVTVVLENGEELVTDMVLISIGRVLNTKDIGLEEVGVALGPKGEVLVDEYLRTNVENIYAIGDITSKMQLAHVASAQGIR
VVENLVGEPQPMSYDVVPGCIFTLPEIAMVGLTSQEAEEKGIKIITGKFPFQASGKAVAMEETEGFVKIIADFYTHRILG
GHIVGPHATDLIGEIALAVQKGLTLEEVAHTIHAHPSLPEAVMEAAEAALNRAIHI

Sequences:

>Translated_456_residues
MYDVAILGGGPGGYVAAIRAAQLGLSVVVVEKDELGGTCLNRGCIPTKALVSTAERLHQIKNSAAFGIEVTGYNFNFQKA
AERKNQVVERLVKGIHYLFKKSKITLIKGTGKLTGKNEITVETSDGLEKVEAKNIILATGSKPALISALGYDGERVITSD
EALNLEKLPAEMVIIGGGVIGSEFATIFSEMGVKVTIVELLPSILANTDKEVSRYLTTLFKKRGIQVKTKVAVKEVKKGE
KVTVVLENGEELVTDMVLISIGRVLNTKDIGLEEVGVALGPKGEVLVDEYLRTNVENIYAIGDITSKMQLAHVASAQGIR
VVENLVGEPQPMSYDVVPGCIFTLPEIAMVGLTSQEAEEKGIKIITGKFPFQASGKAVAMEETEGFVKIIADFYTHRILG
GHIVGPHATDLIGEIALAVQKGLTLEEVAHTIHAHPSLPEAVMEAAEAALNRAIHI
>Mature_456_residues
MYDVAILGGGPGGYVAAIRAAQLGLSVVVVEKDELGGTCLNRGCIPTKALVSTAERLHQIKNSAAFGIEVTGYNFNFQKA
AERKNQVVERLVKGIHYLFKKSKITLIKGTGKLTGKNEITVETSDGLEKVEAKNIILATGSKPALISALGYDGERVITSD
EALNLEKLPAEMVIIGGGVIGSEFATIFSEMGVKVTIVELLPSILANTDKEVSRYLTTLFKKRGIQVKTKVAVKEVKKGE
KVTVVLENGEELVTDMVLISIGRVLNTKDIGLEEVGVALGPKGEVLVDEYLRTNVENIYAIGDITSKMQLAHVASAQGIR
VVENLVGEPQPMSYDVVPGCIFTLPEIAMVGLTSQEAEEKGIKIITGKFPFQASGKAVAMEETEGFVKIIADFYTHRILG
GHIVGPHATDLIGEIALAVQKGLTLEEVAHTIHAHPSLPEAVMEAAEAALNRAIHI

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=459, Percent_Identity=41.6122004357298, Blast_Score=352, Evalue=4e-97,
Organism=Homo sapiens, GI50301238, Length=454, Percent_Identity=33.7004405286344, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI291045266, Length=434, Percent_Identity=28.3410138248848, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI33519430, Length=450, Percent_Identity=27.5555555555556, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI33519428, Length=450, Percent_Identity=27.5555555555556, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI33519426, Length=450, Percent_Identity=27.5555555555556, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI148277065, Length=450, Percent_Identity=28.2222222222222, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI148277071, Length=450, Percent_Identity=28.2222222222222, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI22035672, Length=459, Percent_Identity=28.1045751633987, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI291045268, Length=424, Percent_Identity=26.8867924528302, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI21389617, Length=228, Percent_Identity=27.1929824561404, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI226437568, Length=228, Percent_Identity=27.1929824561404, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI65787454, Length=228, Percent_Identity=27.1929824561404, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1786307, Length=450, Percent_Identity=38.4444444444444, Blast_Score=307, Evalue=8e-85,
Organism=Escherichia coli, GI87081717, Length=455, Percent_Identity=29.8901098901099, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI87082354, Length=459, Percent_Identity=27.6688453159041, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI1789915, Length=431, Percent_Identity=29.0023201856148, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1788892, Length=215, Percent_Identity=30.2325581395349, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI32565766, Length=458, Percent_Identity=39.9563318777293, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI17557007, Length=474, Percent_Identity=28.9029535864979, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI71983429, Length=433, Percent_Identity=29.5612009237875, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI71983419, Length=433, Percent_Identity=29.5612009237875, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI71982272, Length=434, Percent_Identity=24.1935483870968, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17559934, Length=230, Percent_Identity=29.5652173913043, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6321091, Length=475, Percent_Identity=40.8421052631579, Blast_Score=322, Evalue=7e-89,
Organism=Saccharomyces cerevisiae, GI6325240, Length=476, Percent_Identity=29.4117647058824, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6325166, Length=466, Percent_Identity=28.3261802575107, Blast_Score=171, Evalue=3e-43,
Organism=Drosophila melanogaster, GI21358499, Length=459, Percent_Identity=40.5228758169935, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24640549, Length=470, Percent_Identity=27.8723404255319, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24640553, Length=470, Percent_Identity=27.8723404255319, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24640551, Length=470, Percent_Identity=27.8723404255319, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI17737741, Length=471, Percent_Identity=26.1146496815287, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48771; Mature: 48771

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDVAILGGGPGGYVAAIRAAQLGLSVVVVEKDELGGTCLNRGCIPTKALVSTAERLHQI
CEEEEEEECCCCHHHHHHHHHHCCEEEEEEECCCCCCHHHCCCCCCHHHHHHHHHHHHHH
KNSAAFGIEVTGYNFNFQKAAERKNQVVERLVKGIHYLFKKSKITLIKGTGKLTGKNEIT
HCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEE
VETSDGLEKVEAKNIILATGSKPALISALGYDGERVITSDEALNLEKLPAEMVIIGGGVI
EECCCCHHHHHHCEEEEECCCCCCEEEEECCCCCEEEECCCCCCHHHCCCEEEEEECCCH
GSEFATIFSEMGVKVTIVELLPSILANTDKEVSRYLTTLFKKRGIQVKTKVAVKEVKKGE
HHHHHHHHHHCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHCCCC
KVTVVLENGEELVTDMVLISIGRVLNTKDIGLEEVGVALGPKGEVLVDEYLRTNVENIYA
EEEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHCEEECCCCCHHHHHHHHCCHHHEEE
IGDITSKMQLAHVASAQGIRVVENLVGEPQPMSYDVVPGCIFTLPEIAMVGLTSQEAEEK
EECHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHC
GIKIITGKFPFQASGKAVAMEETEGFVKIIADFYTHRILGGHIVGPHATDLIGEIALAVQ
CCEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHH
KGLTLEEVAHTIHAHPSLPEAVMEAAEAALNRAIHI
HCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYDVAILGGGPGGYVAAIRAAQLGLSVVVVEKDELGGTCLNRGCIPTKALVSTAERLHQI
CEEEEEEECCCCHHHHHHHHHHCCEEEEEEECCCCCCHHHCCCCCCHHHHHHHHHHHHHH
KNSAAFGIEVTGYNFNFQKAAERKNQVVERLVKGIHYLFKKSKITLIKGTGKLTGKNEIT
HCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEE
VETSDGLEKVEAKNIILATGSKPALISALGYDGERVITSDEALNLEKLPAEMVIIGGGVI
EECCCCHHHHHHCEEEEECCCCCCEEEEECCCCCEEEECCCCCCHHHCCCEEEEEECCCH
GSEFATIFSEMGVKVTIVELLPSILANTDKEVSRYLTTLFKKRGIQVKTKVAVKEVKKGE
HHHHHHHHHHCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHCCCC
KVTVVLENGEELVTDMVLISIGRVLNTKDIGLEEVGVALGPKGEVLVDEYLRTNVENIYA
EEEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHCEEECCCCCHHHHHHHHCCHHHEEE
IGDITSKMQLAHVASAQGIRVVENLVGEPQPMSYDVVPGCIFTLPEIAMVGLTSQEAEEK
EECHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHC
GIKIITGKFPFQASGKAVAMEETEGFVKIIADFYTHRILGGHIVGPHATDLIGEIALAVQ
CCEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHH
KGLTLEEVAHTIHAHPSLPEAVMEAAEAALNRAIHI
HCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]