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The map label for this gene is folC [H]

Identifier: 78044302

GI number: 78044302

Start: 290771

End: 292069

Strand: Direct

Name: folC [H]

Synonym: CHY_0335

Alternate gene names: 78044302

Gene position: 290771-292069 (Clockwise)

Preceding gene: 78044410

Following gene: 78043444

Centisome position: 12.11

GC content: 44.96

Gene sequence:

>1299_bases
ATGAACTATAACGAAGCGCTGGAATTTTTAAGTAACCTTACCAAATTTGGAATTAATTTGGGTTTAGGGAGGATTTCTGA
ACTTTTAAAAAGGCTTGGCAATCCGCAGGAAAAGGTAAAGGTAGTTCACATCGGTGGAACAAACGGTAAGGGGTCGGTTT
GTTCGTTAACGGCTTCGGTGTTGGCGGAGGCGGGGTATAAAACGGGGCTTTTTACCTCGCCCCACCTTTCCCGGTATACC
GAACGCTATAAAATAAACGGTAAAGAAATTTCGCCGGAAAGGGTTTCTCAGCTGGTGACGGAAATAAGACCGTTACTTTT
GGCGATGGTAGAGGAAGGTTTTGAACATCCTACCGAGTTTGAAGTGTCCACGGCGATGGCTTTTAAGTATTTTGCCGAAG
AGGAAGTTGATTTTGTGGTTTTAGAAGTAGGACTGGGAGGCTTAATTGATTCTACCAATGTGGTTTCCCGTCCGGAAGTT
GTGGCGATTACCAATGTGGGAATGGACCATATGGATTATCTGGGAAATACCATTACCGAAATTGCCCGGGTTAAGGCGGG
GATAATAAAGCCGGGAGTGCCGGTGGTAACCGCGGCAACAGGGGAGGCTCTGGAAGTTATCAAAGAGGTAGCAAAAGAGA
AAAACAGTCCCCTTTTGGATGTTAATAAGGACATCGTTTTAAATTTAAAAGAAAAAAGCTTAAAAGGGCAGGTTTTTGAC
CTTATTTACCGGGGTGAAACCTATCCAGAGATAAAAATCCGCCTTTTAGGAGCCCATCAGCTTTTAAATGCCAAAACTTC
TTTTGGTATTCTTAATATCCTGCGGGAAAAATATAACATACCGGTGGAAGCAATTTACCGGGGATTTAGCAACGCCACCT
GGTCGGGGCGTTTTGAGTATATTCCGGGAAATCCGCCGGTACTTCTCGATGGGGCGCACAATCACGATGGAGTTAGAGCT
TTAAGGCAAGCTTTTACTGACTATTTTCCCGGTGTAAAGCCGGTACTGCTCCTGGGAGTTTTAGCCGATAAAGAGCGGGA
AAAAGTGGTGGCGGAGCTTTTAGATTTACCCTTAGAAGTGGTAGTGACCCGACCGCCAACTCCCCGAGCAGGAGATATCA
CCGTCTTAGCAAGGCTTTTTAAAGAAAAAGGGGTTAGGGTGAAGGTTATCGAAGAACCGACCGAAGCTTTATCTTACGGC
CTGGAAATTGCGCAAGAATACCAAACCCTCTTATTAATTACCGGTTCCCTTTATATGATTGGCGAAATCCGCCCTTTACT
TTTGCAGGATAGAGATTAG

Upstream 100 bases:

>100_bases
GCTAAAGCTCCGGCAGAAGTGGTCAATAAAGAGCGGGAAAAGCTAACGGCCTTTTACCGGGAAAAGGAAGTCTTGGAACA
AAGGATAGGGATGCTTTCCC

Downstream 100 bases:

>100_bases
ACGGGCTTTTGCCCGTTTTTTCTTTTTTATTTGTGAAGGTATAGCTGTTTTTACCGACCACTGACCACCGACGACTTACG
ACCGTTAATGCATATTCTTT

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MNYNEALEFLSNLTKFGINLGLGRISELLKRLGNPQEKVKVVHIGGTNGKGSVCSLTASVLAEAGYKTGLFTSPHLSRYT
ERYKINGKEISPERVSQLVTEIRPLLLAMVEEGFEHPTEFEVSTAMAFKYFAEEEVDFVVLEVGLGGLIDSTNVVSRPEV
VAITNVGMDHMDYLGNTITEIARVKAGIIKPGVPVVTAATGEALEVIKEVAKEKNSPLLDVNKDIVLNLKEKSLKGQVFD
LIYRGETYPEIKIRLLGAHQLLNAKTSFGILNILREKYNIPVEAIYRGFSNATWSGRFEYIPGNPPVLLDGAHNHDGVRA
LRQAFTDYFPGVKPVLLLGVLADKEREKVVAELLDLPLEVVVTRPPTPRAGDITVLARLFKEKGVRVKVIEEPTEALSYG
LEIAQEYQTLLLITGSLYMIGEIRPLLLQDRD

Sequences:

>Translated_432_residues
MNYNEALEFLSNLTKFGINLGLGRISELLKRLGNPQEKVKVVHIGGTNGKGSVCSLTASVLAEAGYKTGLFTSPHLSRYT
ERYKINGKEISPERVSQLVTEIRPLLLAMVEEGFEHPTEFEVSTAMAFKYFAEEEVDFVVLEVGLGGLIDSTNVVSRPEV
VAITNVGMDHMDYLGNTITEIARVKAGIIKPGVPVVTAATGEALEVIKEVAKEKNSPLLDVNKDIVLNLKEKSLKGQVFD
LIYRGETYPEIKIRLLGAHQLLNAKTSFGILNILREKYNIPVEAIYRGFSNATWSGRFEYIPGNPPVLLDGAHNHDGVRA
LRQAFTDYFPGVKPVLLLGVLADKEREKVVAELLDLPLEVVVTRPPTPRAGDITVLARLFKEKGVRVKVIEEPTEALSYG
LEIAQEYQTLLLITGSLYMIGEIRPLLLQDRD
>Mature_432_residues
MNYNEALEFLSNLTKFGINLGLGRISELLKRLGNPQEKVKVVHIGGTNGKGSVCSLTASVLAEAGYKTGLFTSPHLSRYT
ERYKINGKEISPERVSQLVTEIRPLLLAMVEEGFEHPTEFEVSTAMAFKYFAEEEVDFVVLEVGLGGLIDSTNVVSRPEV
VAITNVGMDHMDYLGNTITEIARVKAGIIKPGVPVVTAATGEALEVIKEVAKEKNSPLLDVNKDIVLNLKEKSLKGQVFD
LIYRGETYPEIKIRLLGAHQLLNAKTSFGILNILREKYNIPVEAIYRGFSNATWSGRFEYIPGNPPVLLDGAHNHDGVRA
LRQAFTDYFPGVKPVLLLGVLADKEREKVVAELLDLPLEVVVTRPPTPRAGDITVLARLFKEKGVRVKVIEEPTEALSYG
LEIAQEYQTLLLITGSLYMIGEIRPLLLQDRD

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=382, Percent_Identity=28.5340314136126, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI66932990, Length=326, Percent_Identity=30.6748466257669, Blast_Score=154, Evalue=2e-37,
Organism=Escherichia coli, GI1788654, Length=417, Percent_Identity=29.4964028776978, Blast_Score=143, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17553148, Length=433, Percent_Identity=26.7898383371824, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI71984923, Length=433, Percent_Identity=26.7898383371824, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17553150, Length=433, Percent_Identity=26.7898383371824, Blast_Score=147, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6323760, Length=436, Percent_Identity=33.4862385321101, Blast_Score=203, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6324815, Length=333, Percent_Identity=32.1321321321321, Blast_Score=140, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6322718, Length=286, Percent_Identity=23.7762237762238, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24641571, Length=311, Percent_Identity=34.4051446945338, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24581568, Length=251, Percent_Identity=30.2788844621514, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47739; Mature: 47739

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYNEALEFLSNLTKFGINLGLGRISELLKRLGNPQEKVKVVHIGGTNGKGSVCSLTASV
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHEEEEEEECCCCCCCHHHHHHHH
LAEAGYKTGLFTSPHLSRYTERYKINGKEISPERVSQLVTEIRPLLLAMVEEGFEHPTEF
HHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EVSTAMAFKYFAEEEVDFVVLEVGLGGLIDSTNVVSRPEVVAITNVGMDHMDYLGNTITE
CHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHH
IARVKAGIIKPGVPVVTAATGEALEVIKEVAKEKNSPLLDVNKDIVLNLKEKSLKGQVFD
HHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHCCCCEEECCCCEEEEEHHHHHCCHHHH
LIYRGETYPEIKIRLLGAHQLLNAKTSFGILNILREKYNIPVEAIYRGFSNATWSGRFEY
HEECCCCCCCEEEEEEEHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEE
IPGNPPVLLDGAHNHDGVRALRQAFTDYFPGVKPVLLLGVLADKEREKVVAELLDLPLEV
CCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHCCCEEE
VVTRPPTPRAGDITVLARLFKEKGVRVKVIEEPTEALSYGLEIAQEYQTLLLITGSLYMI
EEECCCCCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHEEEEEECCEEEE
GEIRPLLLQDRD
ECCCEEEECCCC
>Mature Secondary Structure
MNYNEALEFLSNLTKFGINLGLGRISELLKRLGNPQEKVKVVHIGGTNGKGSVCSLTASV
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHEEEEEEECCCCCCCHHHHHHHH
LAEAGYKTGLFTSPHLSRYTERYKINGKEISPERVSQLVTEIRPLLLAMVEEGFEHPTEF
HHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EVSTAMAFKYFAEEEVDFVVLEVGLGGLIDSTNVVSRPEVVAITNVGMDHMDYLGNTITE
CHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHH
IARVKAGIIKPGVPVVTAATGEALEVIKEVAKEKNSPLLDVNKDIVLNLKEKSLKGQVFD
HHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHCCCCEEECCCCEEEEEHHHHHCCHHHH
LIYRGETYPEIKIRLLGAHQLLNAKTSFGILNILREKYNIPVEAIYRGFSNATWSGRFEY
HEECCCCCCCEEEEEEEHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEE
IPGNPPVLLDGAHNHDGVRALRQAFTDYFPGVKPVLLLGVLADKEREKVVAELLDLPLEV
CCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHCCCEEE
VVTRPPTPRAGDITVLARLFKEKGVRVKVIEEPTEALSYGLEIAQEYQTLLLITGSLYMI
EEECCCCCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHEEEEEECCEEEE
GEIRPLLLQDRD
ECCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]