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The map label for this gene is ppdK [H]

Identifier: 78044221

GI number: 78044221

Start: 387697

End: 390354

Strand: Direct

Name: ppdK [H]

Synonym: CHY_0443

Alternate gene names: 78044221

Gene position: 387697-390354 (Clockwise)

Preceding gene: 78044534

Following gene: 78043254

Centisome position: 16.14

GC content: 47.33

Gene sequence:

>2658_bases
ATGACCAAATACGTTTACTTATTTCATGAAGGCCGCGCTGACATGAAAGATTTATTGGGGGGAAAAGGGGCCAATTTAGC
GGAAATGACCAATATCGGTCTTCCGGTACCTCCGGGAATGACCATTACTACCGAAGCCTGTCGGGAGTATTACCGGCTGG
GAGGTAAGTTCCCGGAAGGTTTAATGGAAGAGGTCAAGGAAAAGCTTGTTTATATTGAAGAAAAAACCGGTAAAAAATTT
GGCGACCCCCAAAACCCTCTTTTAGTTTCGGTGCGGTCGGGAGCTAAATTCTCGATGCCGGGAATGATGGATACTATCTT
AAACCTCGGTTTAAACGAGGAAACGGTGGAAGGTTTGGCGCAAAACACTCAAAATCCCCGGTTTGCTTATGATGCTTACC
GGCGGTTTATTCAGATGTTTGGGGATGTGGTTTTAGAAATTCCCAAACACGAGTTTGAACATATTTTAGACCGGCAAAAA
GAAAAGGAAGGGGTAACCTTTGACCAGGAACTTTCCGCAGAAGCTTTAAAGGAAGTTATTACCCGATATAAAGAATTGGT
AGAAAGAAAAACTGGAAAGCCTTTCCCTTCTGATCCCATGGTTCAGCTTACCATGGCTATTGAAGCGGTATTTAAGTCCT
GGAATAACGACCGGGCCATTGTCTACCGGAATTTAAATAAAATTCCCCATGATTTGGGTACTGCGGTTAACATCCAGTCA
ATGGTCTTTGGTAATATGGGCAACGACTCCGGTACCGGTGTGGCCTTTACCCGGAACCCTTCCACCGGGGAAAAGGTTTT
ATACGGTGAATATCTGACCAACGCTCAAGGAGAAGATGTGGTTGCCGGCATTCGTACTCCGAGTCCCATTTCCAAGTTAA
AAGAAGAAATGCCGGAAGTTTACGAACAATTTGTCAGTATTGCTAAACTCTTAGAAAGTCACTACAAAAACATGCAGGAC
ATTGAATTTACCATTGAGCGGGGTAAACTTTATATCCTGCAAACCAGAAACGGCAAGCGGACGGCCCAGGCTGCAGTTAA
GATTGCCCATGACATGGTCGAAGAAGGGTTAATTACCAAAAAAGAAGCGATTTTAATGGTGGAGCCGGGACAATTAGACC
AGCTTCTTCACCGGCAAATTGATCCTTCGGCCAAGGTGGAGGTAATTGCCAAGGGGTTACCCGCTTCTCCCGGGGCTGCC
TCGGGGATTGTAGTTTTTGATGCCGATGAAGCGGAGAAACTGGGCAAAGAAGGGAAAAAGGTTTTACTGGTGCGGACCGA
AACCACTCCCGATGATATCCACGGCATTGTGGCCGCCCAGGGAGTTTTAACCAGCCGGGGTGGTATGACCAGCCATGCGG
CGGTGGTTGCCCGGGGCATGGGTAAGCCGTGTGTTTGCGGTTGTGAAGCCATCAAAATTGATTATGAGAAAAAATTGTTT
ACCGTAGACAATATTACCGTAAAAGAAGGAGACTACTTATCCATTGACGGTTCTACCGGACGGGTAATTCTCGGAACTGT
CCCGATGAAAGATCCAGAACTATCTCCGGAATTTATTAAGCTTCTGGAATGGGCCGATGAGTTAAAGCGGCTTGAGGTTC
GGGCCAATGCCGATACCCCGGAAGACGCCCAGAAAGCCCGGGAATTTGGAGCCAAAGGTATCGGCCTTTGCCGGACGGAA
CATATGTTTATGGGCCCCGAGCGGCTGCCTCACGTACAAAGAATGATTTTAGCCGAAACCAAAGAAGAGCGGGAAGAGGC
TCTATCTCACCTTCTTCCCATGCAGGAAGAGGATTTCTATGGCATTTTAAAAGCTATGGAAGGCTATCCGGTATGTATCC
GGCTCCTGGACCCGCCCCTTCATGAATTTTTGCCAAGTTTAGAAGAACTGTTGGTGGAAACTACCGAGCTCAGGGTGCGG
GGAGATAATCCAGAATTACTTGCCGAAAAAGAAGCTCTTTTAAAGAAAGTAAAATCTTTACACGAATTTAACCCGATGCT
CGGACACCGGGGCTGCCGTTTGGGGATTACTTATCCGGAAGTTTACGAAATGCAGATTCGGGCCATCTTTAACGCTGCTG
CTCGCTTAACCAAAGAAGGGTATAAAGTATATCCTGAGGTGGAAATTCCCTTAACCATTGATGTTAACGAAATGAAATTC
TTTAAAGAAAGAATTGATGCCATTGCCCGGGAGGTAATGGAAAGGGAAGGGGTTACCTTCCACTACACCACCGGCACCAT
GATTGAATTACCAAGGGCAGCGCTACTGGCCGATGAACTGGCCGAAGTTGCCGAATTCTTTAGCTTTGGTACTAACGACC
TAACCCAGACTACCCTTGGTTTTAGCCGGGACGACGCCGAAGGGAAGTTTTTAACCCACTACTTAGATATGAAGATTTTA
AAGGAAAATCCCTTTATCGTCTTGGACCGCAAAGGTGTTGGGAAATTAATGAAAATTGCCGTAGAAGGCGGCCGGAAAAC
CCGTCCGGATTTACTGGTCGGTATCTGCGGTGAGCACGGCGGCGAACCAAGCTCCGTAGAATTCTGCCACCAGATTGGGC
TTGACTTTGTAAGCTGTTCACCTTACCGGGTGCCGATTGCCCGCCTGGCAGCAGCTCAGGCGGCGGTGGCGGAAGGAGAA
ATTGTTGGAACTAAATAA

Upstream 100 bases:

>100_bases
TGAAAAAAATCGGCTGTGCCGTAATTGATGTGAGTAATAAAGCGGTGGAGGAAACGGCAGCCAGGGTGTTAGAAATTTAC
AGGAAAGGGGTAGGGAGGTA

Downstream 100 bases:

>100_bases
GGAAATTTAAAAATAAAGGGGCTACCGCAAAAAAATAAATGGGTAGCCCCTTATTTAATGTTTTGGAACTTTTTTGTTCT
AATCCTCTCTAATCTAATAT

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 885; Mature: 884

Protein sequence:

>885_residues
MTKYVYLFHEGRADMKDLLGGKGANLAEMTNIGLPVPPGMTITTEACREYYRLGGKFPEGLMEEVKEKLVYIEEKTGKKF
GDPQNPLLVSVRSGAKFSMPGMMDTILNLGLNEETVEGLAQNTQNPRFAYDAYRRFIQMFGDVVLEIPKHEFEHILDRQK
EKEGVTFDQELSAEALKEVITRYKELVERKTGKPFPSDPMVQLTMAIEAVFKSWNNDRAIVYRNLNKIPHDLGTAVNIQS
MVFGNMGNDSGTGVAFTRNPSTGEKVLYGEYLTNAQGEDVVAGIRTPSPISKLKEEMPEVYEQFVSIAKLLESHYKNMQD
IEFTIERGKLYILQTRNGKRTAQAAVKIAHDMVEEGLITKKEAILMVEPGQLDQLLHRQIDPSAKVEVIAKGLPASPGAA
SGIVVFDADEAEKLGKEGKKVLLVRTETTPDDIHGIVAAQGVLTSRGGMTSHAAVVARGMGKPCVCGCEAIKIDYEKKLF
TVDNITVKEGDYLSIDGSTGRVILGTVPMKDPELSPEFIKLLEWADELKRLEVRANADTPEDAQKAREFGAKGIGLCRTE
HMFMGPERLPHVQRMILAETKEEREEALSHLLPMQEEDFYGILKAMEGYPVCIRLLDPPLHEFLPSLEELLVETTELRVR
GDNPELLAEKEALLKKVKSLHEFNPMLGHRGCRLGITYPEVYEMQIRAIFNAAARLTKEGYKVYPEVEIPLTIDVNEMKF
FKERIDAIAREVMEREGVTFHYTTGTMIELPRAALLADELAEVAEFFSFGTNDLTQTTLGFSRDDAEGKFLTHYLDMKIL
KENPFIVLDRKGVGKLMKIAVEGGRKTRPDLLVGICGEHGGEPSSVEFCHQIGLDFVSCSPYRVPIARLAAAQAAVAEGE
IVGTK

Sequences:

>Translated_885_residues
MTKYVYLFHEGRADMKDLLGGKGANLAEMTNIGLPVPPGMTITTEACREYYRLGGKFPEGLMEEVKEKLVYIEEKTGKKF
GDPQNPLLVSVRSGAKFSMPGMMDTILNLGLNEETVEGLAQNTQNPRFAYDAYRRFIQMFGDVVLEIPKHEFEHILDRQK
EKEGVTFDQELSAEALKEVITRYKELVERKTGKPFPSDPMVQLTMAIEAVFKSWNNDRAIVYRNLNKIPHDLGTAVNIQS
MVFGNMGNDSGTGVAFTRNPSTGEKVLYGEYLTNAQGEDVVAGIRTPSPISKLKEEMPEVYEQFVSIAKLLESHYKNMQD
IEFTIERGKLYILQTRNGKRTAQAAVKIAHDMVEEGLITKKEAILMVEPGQLDQLLHRQIDPSAKVEVIAKGLPASPGAA
SGIVVFDADEAEKLGKEGKKVLLVRTETTPDDIHGIVAAQGVLTSRGGMTSHAAVVARGMGKPCVCGCEAIKIDYEKKLF
TVDNITVKEGDYLSIDGSTGRVILGTVPMKDPELSPEFIKLLEWADELKRLEVRANADTPEDAQKAREFGAKGIGLCRTE
HMFMGPERLPHVQRMILAETKEEREEALSHLLPMQEEDFYGILKAMEGYPVCIRLLDPPLHEFLPSLEELLVETTELRVR
GDNPELLAEKEALLKKVKSLHEFNPMLGHRGCRLGITYPEVYEMQIRAIFNAAARLTKEGYKVYPEVEIPLTIDVNEMKF
FKERIDAIAREVMEREGVTFHYTTGTMIELPRAALLADELAEVAEFFSFGTNDLTQTTLGFSRDDAEGKFLTHYLDMKIL
KENPFIVLDRKGVGKLMKIAVEGGRKTRPDLLVGICGEHGGEPSSVEFCHQIGLDFVSCSPYRVPIARLAAAQAAVAEGE
IVGTK
>Mature_884_residues
TKYVYLFHEGRADMKDLLGGKGANLAEMTNIGLPVPPGMTITTEACREYYRLGGKFPEGLMEEVKEKLVYIEEKTGKKFG
DPQNPLLVSVRSGAKFSMPGMMDTILNLGLNEETVEGLAQNTQNPRFAYDAYRRFIQMFGDVVLEIPKHEFEHILDRQKE
KEGVTFDQELSAEALKEVITRYKELVERKTGKPFPSDPMVQLTMAIEAVFKSWNNDRAIVYRNLNKIPHDLGTAVNIQSM
VFGNMGNDSGTGVAFTRNPSTGEKVLYGEYLTNAQGEDVVAGIRTPSPISKLKEEMPEVYEQFVSIAKLLESHYKNMQDI
EFTIERGKLYILQTRNGKRTAQAAVKIAHDMVEEGLITKKEAILMVEPGQLDQLLHRQIDPSAKVEVIAKGLPASPGAAS
GIVVFDADEAEKLGKEGKKVLLVRTETTPDDIHGIVAAQGVLTSRGGMTSHAAVVARGMGKPCVCGCEAIKIDYEKKLFT
VDNITVKEGDYLSIDGSTGRVILGTVPMKDPELSPEFIKLLEWADELKRLEVRANADTPEDAQKAREFGAKGIGLCRTEH
MFMGPERLPHVQRMILAETKEEREEALSHLLPMQEEDFYGILKAMEGYPVCIRLLDPPLHEFLPSLEELLVETTELRVRG
DNPELLAEKEALLKKVKSLHEFNPMLGHRGCRLGITYPEVYEMQIRAIFNAAARLTKEGYKVYPEVEIPLTIDVNEMKFF
KERIDAIAREVMEREGVTFHYTTGTMIELPRAALLADELAEVAEFFSFGTNDLTQTTLGFSRDDAEGKFLTHYLDMKILK
ENPFIVLDRKGVGKLMKIAVEGGRKTRPDLLVGICGEHGGEPSSVEFCHQIGLDFVSCSPYRVPIARLAAAQAAVAEGEI
VGTK

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=465, Percent_Identity=25.1612903225806, Blast_Score=113, Evalue=6e-26,
Organism=Escherichia coli, GI48994992, Length=453, Percent_Identity=24.5033112582781, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1787994, Length=715, Percent_Identity=23.7762237762238, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1789193, Length=466, Percent_Identity=23.8197424892704, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1788726, Length=467, Percent_Identity=23.1263383297645, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 98601; Mature: 98470

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYVYLFHEGRADMKDLLGGKGANLAEMTNIGLPVPPGMTITTEACREYYRLGGKFPEG
CCEEEEEEECCCCCHHHHHCCCCCCHHHHHCCCCCCCCCCEEEHHHHHHHHHCCCCCCHH
LMEEVKEKLVYIEEKTGKKFGDPQNPLLVSVRSGAKFSMPGMMDTILNLGLNEETVEGLA
HHHHHHHHHEEEEHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHH
QNTQNPRFAYDAYRRFIQMFGDVVLEIPKHEFEHILDRQKEKEGVTFDQELSAEALKEVI
HCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
TRYKELVERKTGKPFPSDPMVQLTMAIEAVFKSWNNDRAIVYRNLNKIPHDLGTAVNIQS
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCHHCCHHHCCCEEEHH
MVFGNMGNDSGTGVAFTRNPSTGEKVLYGEYLTNAQGEDVVAGIRTPSPISKLKEEMPEV
HHCCCCCCCCCCEEEEECCCCCCCEEEEEHHHCCCCCCCEEEECCCCCHHHHHHHHHHHH
YEQFVSIAKLLESHYKNMQDIEFTIERGKLYILQTRNGKRTAQAAVKIAHDMVEEGLITK
HHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCC
KEAILMVEPGQLDQLLHRQIDPSAKVEVIAKGLPASPGAASGIVVFDADEAEKLGKEGKK
CCEEEEECCCHHHHHHHHHCCCCCCHHHEECCCCCCCCCCCCEEEEECCHHHHHCCCCCE
VLLVRTETTPDDIHGIVAAQGVLTSRGGMTSHAAVVARGMGKPCVCGCEAIKIDYEKKLF
EEEEEECCCCHHHHHHHHHCCHHCCCCCCCHHHHHHHHCCCCCCEECCEEEEEECCCEEE
TVDNITVKEGDYLSIDGSTGRVILGTVPMKDPELSPEFIKLLEWADELKRLEVRANADTP
EECCEEEECCCEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCC
EDAQKAREFGAKGIGLCRTEHMFMGPERLPHVQRMILAETKEEREEALSHLLPMQEEDFY
HHHHHHHHHCCCCCCEEECCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
GILKAMEGYPVCIRLLDPPLHEFLPSLEELLVETTELRVRGDNPELLAEKEALLKKVKSL
HHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHH
HEFNPMLGHRGCRLGITYPEVYEMQIRAIFNAAARLTKEGYKVYPEVEIPLTIDVNEMKF
HHCCHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCEECCEEEEEEEEEHHHHHH
FKERIDAIAREVMEREGVTFHYTTGTMIELPRAALLADELAEVAEFFSFGTNDLTQTTLG
HHHHHHHHHHHHHHHCCCEEEEECCCHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCC
FSRDDAEGKFLTHYLDMKILKENPFIVLDRKGVGKLMKIAVEGGRKTRPDLLVGICGEHG
CCCCCCCCCHHHHHHHHHEECCCCEEEEECCCCCHHHHHHHCCCCCCCCCEEEEECCCCC
GEPSSVEFCHQIGLDFVSCSPYRVPIARLAAAQAAVAEGEIVGTK
CCCCHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHCCCEECCC
>Mature Secondary Structure 
TKYVYLFHEGRADMKDLLGGKGANLAEMTNIGLPVPPGMTITTEACREYYRLGGKFPEG
CEEEEEEECCCCCHHHHHCCCCCCHHHHHCCCCCCCCCCEEEHHHHHHHHHCCCCCCHH
LMEEVKEKLVYIEEKTGKKFGDPQNPLLVSVRSGAKFSMPGMMDTILNLGLNEETVEGLA
HHHHHHHHHEEEEHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHH
QNTQNPRFAYDAYRRFIQMFGDVVLEIPKHEFEHILDRQKEKEGVTFDQELSAEALKEVI
HCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
TRYKELVERKTGKPFPSDPMVQLTMAIEAVFKSWNNDRAIVYRNLNKIPHDLGTAVNIQS
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCHHCCHHHCCCEEEHH
MVFGNMGNDSGTGVAFTRNPSTGEKVLYGEYLTNAQGEDVVAGIRTPSPISKLKEEMPEV
HHCCCCCCCCCCEEEEECCCCCCCEEEEEHHHCCCCCCCEEEECCCCCHHHHHHHHHHHH
YEQFVSIAKLLESHYKNMQDIEFTIERGKLYILQTRNGKRTAQAAVKIAHDMVEEGLITK
HHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCC
KEAILMVEPGQLDQLLHRQIDPSAKVEVIAKGLPASPGAASGIVVFDADEAEKLGKEGKK
CCEEEEECCCHHHHHHHHHCCCCCCHHHEECCCCCCCCCCCCEEEEECCHHHHHCCCCCE
VLLVRTETTPDDIHGIVAAQGVLTSRGGMTSHAAVVARGMGKPCVCGCEAIKIDYEKKLF
EEEEEECCCCHHHHHHHHHCCHHCCCCCCCHHHHHHHHCCCCCCEECCEEEEEECCCEEE
TVDNITVKEGDYLSIDGSTGRVILGTVPMKDPELSPEFIKLLEWADELKRLEVRANADTP
EECCEEEECCCEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCC
EDAQKAREFGAKGIGLCRTEHMFMGPERLPHVQRMILAETKEEREEALSHLLPMQEEDFY
HHHHHHHHHCCCCCCEEECCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
GILKAMEGYPVCIRLLDPPLHEFLPSLEELLVETTELRVRGDNPELLAEKEALLKKVKSL
HHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHH
HEFNPMLGHRGCRLGITYPEVYEMQIRAIFNAAARLTKEGYKVYPEVEIPLTIDVNEMKF
HHCCHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCEECCEEEEEEEEEHHHHHH
FKERIDAIAREVMEREGVTFHYTTGTMIELPRAALLADELAEVAEFFSFGTNDLTQTTLG
HHHHHHHHHHHHHHHCCCEEEEECCCHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCC
FSRDDAEGKFLTHYLDMKILKENPFIVLDRKGVGKLMKIAVEGGRKTRPDLLVGICGEHG
CCCCCCCCCHHHHHHHHHEECCCCEEEEECCCCCHHHHHHHCCCCCCCCCEEEEECCCCC
GEPSSVEFCHQIGLDFVSCSPYRVPIARLAAAQAAVAEGEIVGTK
CCCCHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 9168612 [H]