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The map label for this gene is metK

Identifier: 78043882

GI number: 78043882

Start: 120033

End: 121223

Strand: Direct

Name: metK

Synonym: CHY_0138

Alternate gene names: 78043882

Gene position: 120033-121223 (Clockwise)

Preceding gene: 78044420

Following gene: 78043220

Centisome position: 5.0

GC content: 49.87

Gene sequence:

>1191_bases
ATGACAAGACGCCTTTTTACCTCAGAGTCGGTTACCGAAGGACACCCCGATAAAATTGCCGACCAGATTTCCGATGCGGT
GTTAGATGCTATCCTTGCCCAGGACCCTCAGGCCCGGGTGGCGTGTGAAACGGCAGTGACCACCGGCTTGGTCTTGGTTA
TTGGCGAGATTACTACCAGCTGTTATGTGGATATTCCCAAAGTTGTGCGGGAGACAATCCGGGAGATTGGTTATACCCGG
GCAAAATACGGATTTGACGGTGATACCTGTGCCGTTATCACTTCCATAGATGAGCAGTCTCCGGATATTGCTTTAGGGGT
TAACCGGGCCTTAGAAGCCAAGACCGGAGAAATGACTGACGATGAAATTGAAGCCATTGGTGCCGGCGATCAGGGTATGA
TGTTTGGTTATGCAACCAACGAAACCCCGGAACTTATGCCGATGCCCATTTCCTTAGCTCACCGGCTTGCCCGGAGACTG
GCGGAAGTCAGGAAATCCAGGCTTTTACCGTATCTTCGTCCCGATGGGAAAACCCAGGTAACGGTAGAGTATGATGGTGA
TAAGCCTGTACGGGTTGATACCATTGTCGTTTCAACCCAGCATCATCCCGATGTATCCCAGGAGGAAATTCGTAAGGATA
TTATCGAACATGTGGTAAAACCGGTTATTCCGGAGCATCTTTTAGATGAAAACACCCGGTATTTTGTGAATCCCACCGGC
CGTTTTGTAATTGGTGGACCTCAAGGCGATGCCGGACTTACCGGGCGGAAAATCATTGTGGATACTTACGGTGGTATGGC
CCGCCACGGTGGTGGAGCCTTTTCCGGTAAAGACCCCACCAAAGTGGACCGTTCTGCCGCTTATGCTGCCCGGTATGTGG
CGAAAAACATTGTGGCCGCAGGTATCGCCGATAAGATAGAAATCCAGCTGGCTTACGCCATTGGGGTGGCCAAGCCCGTC
TCTATCATGGTGGATACCTTTGGCACCGGCAAAATTGCCGATGAAAAGATTGTAGAGCTCATTAAAAAGCACTTTGATTT
ACGTCCGGCAGGAATAATCCGGATGTTGGATTTACGGCGGCCGATTTACAAGCAAACTGCTGCCTACGGCCATTTTGGTC
GTACCGATATCGATCTCCCCTGGGAAAGAATTGATAAAGCAGAAATTTTAAGAATGGAAGCTGGACTTTAA

Upstream 100 bases:

>100_bases
CATCCTTTGCTAAAGTAAGATAAAGAAATTTTAAAAAAGCACCTCTTCCAGAACAGGGGAGGTTTTCTTTTTGAGAAAAT
AAGAATCAGGAGGAGATTTT

Downstream 100 bases:

>100_bases
ACAGAACCCTGGGCCAGATTAACTGGCCAGGGTTTTAACTTTTTTAACCCCTTTAGCATACGGTATAAGGGGGGTGGCAG
AGGTGATGGAGGCAATCCTG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTRRLFTSESVTEGHPDKIADQISDAVLDAILAQDPQARVACETAVTTGLVLVIGEITTSCYVDIPKVVRETIREIGYTR
AKYGFDGDTCAVITSIDEQSPDIALGVNRALEAKTGEMTDDEIEAIGAGDQGMMFGYATNETPELMPMPISLAHRLARRL
AEVRKSRLLPYLRPDGKTQVTVEYDGDKPVRVDTIVVSTQHHPDVSQEEIRKDIIEHVVKPVIPEHLLDENTRYFVNPTG
RFVIGGPQGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGIADKIEIQLAYAIGVAKPV
SIMVDTFGTGKIADEKIVELIKKHFDLRPAGIIRMLDLRRPIYKQTAAYGHFGRTDIDLPWERIDKAEILRMEAGL

Sequences:

>Translated_396_residues
MTRRLFTSESVTEGHPDKIADQISDAVLDAILAQDPQARVACETAVTTGLVLVIGEITTSCYVDIPKVVRETIREIGYTR
AKYGFDGDTCAVITSIDEQSPDIALGVNRALEAKTGEMTDDEIEAIGAGDQGMMFGYATNETPELMPMPISLAHRLARRL
AEVRKSRLLPYLRPDGKTQVTVEYDGDKPVRVDTIVVSTQHHPDVSQEEIRKDIIEHVVKPVIPEHLLDENTRYFVNPTG
RFVIGGPQGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGIADKIEIQLAYAIGVAKPV
SIMVDTFGTGKIADEKIVELIKKHFDLRPAGIIRMLDLRRPIYKQTAAYGHFGRTDIDLPWERIDKAEILRMEAGL
>Mature_395_residues
TRRLFTSESVTEGHPDKIADQISDAVLDAILAQDPQARVACETAVTTGLVLVIGEITTSCYVDIPKVVRETIREIGYTRA
KYGFDGDTCAVITSIDEQSPDIALGVNRALEAKTGEMTDDEIEAIGAGDQGMMFGYATNETPELMPMPISLAHRLARRLA
EVRKSRLLPYLRPDGKTQVTVEYDGDKPVRVDTIVVSTQHHPDVSQEEIRKDIIEHVVKPVIPEHLLDENTRYFVNPTGR
FVIGGPQGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGIADKIEIQLAYAIGVAKPVS
IMVDTFGTGKIADEKIVELIKKHFDLRPAGIIRMLDLRRPIYKQTAAYGHFGRTDIDLPWERIDKAEILRMEAGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=382, Percent_Identity=57.8534031413613, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI5174529, Length=382, Percent_Identity=58.3769633507853, Blast_Score=432, Evalue=1e-121,
Organism=Escherichia coli, GI1789311, Length=396, Percent_Identity=64.6464646464647, Blast_Score=531, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI17538498, Length=385, Percent_Identity=56.1038961038961, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI25145633, Length=392, Percent_Identity=55.6122448979592, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17538494, Length=385, Percent_Identity=55.3246753246753, Blast_Score=429, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17551082, Length=386, Percent_Identity=54.4041450777202, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI32565905, Length=385, Percent_Identity=50.9090909090909, Blast_Score=373, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32565911, Length=338, Percent_Identity=53.2544378698225, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25145635, Length=344, Percent_Identity=52.9069767441861, Blast_Score=359, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI32565903, Length=340, Percent_Identity=50.5882352941176, Blast_Score=330, Evalue=8e-91,
Organism=Caenorhabditis elegans, GI32565909, Length=85, Percent_Identity=57.6470588235294, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI32565907, Length=38, Percent_Identity=84.2105263157895, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323209, Length=383, Percent_Identity=56.9190600522193, Blast_Score=442, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6320710, Length=389, Percent_Identity=56.0411311053985, Blast_Score=440, Evalue=1e-124,
Organism=Drosophila melanogaster, GI45552159, Length=391, Percent_Identity=55.4987212276215, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580529, Length=391, Percent_Identity=55.4987212276215, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580537, Length=391, Percent_Identity=55.4987212276215, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580533, Length=391, Percent_Identity=55.4987212276215, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580535, Length=391, Percent_Identity=55.4987212276215, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580531, Length=391, Percent_Identity=55.4987212276215, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580527, Length=389, Percent_Identity=54.4987146529563, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580525, Length=389, Percent_Identity=54.4987146529563, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580541, Length=377, Percent_Identity=53.315649867374, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580539, Length=177, Percent_Identity=45.7627118644068, Blast_Score=138, Evalue=6e-33,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_CARHZ (Q3AFS4)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359010.1
- STRING:   Q3AFS4
- GeneID:   3726505
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0138
- NMPDR:   fig|246194.3.peg.882
- TIGR:   CHY_0138
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- BioCyc:   CHYD246194:CHY_0138-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 43426; Mature: 43295

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRLFTSESVTEGHPDKIADQISDAVLDAILAQDPQARVACETAVTTGLVLVIGEITTS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEHHHHH
CYVDIPKVVRETIREIGYTRAKYGFDGDTCAVITSIDEQSPDIALGVNRALEAKTGEMTD
HEECCHHHHHHHHHHCCCCHHCCCCCCCCEEEEEECCCCCCCEEEECCHHHHCCCCCCCC
DEIEAIGAGDQGMMFGYATNETPELMPMPISLAHRLARRLAEVRKSRLLPYLRPDGKTQV
CHHHEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
TVEYDGDKPVRVDTIVVSTQHHPDVSQEEIRKDIIEHVVKPVIPEHLLDENTRYFVNPTG
EEEECCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCHHHHHCCCCEEEECCCC
RFVIGGPQGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAA
CEEEECCCCCCCCCCCEEEEECCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
GIADKIEIQLAYAIGVAKPVSIMVDTFGTGKIADEKIVELIKKHFDLRPAGIIRMLDLRR
CCCCEEEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCC
PIYKQTAAYGHFGRTDIDLPWERIDKAEILRMEAGL
HHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHCCC
>Mature Secondary Structure 
TRRLFTSESVTEGHPDKIADQISDAVLDAILAQDPQARVACETAVTTGLVLVIGEITTS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEHHHHH
CYVDIPKVVRETIREIGYTRAKYGFDGDTCAVITSIDEQSPDIALGVNRALEAKTGEMTD
HEECCHHHHHHHHHHCCCCHHCCCCCCCCEEEEEECCCCCCCEEEECCHHHHCCCCCCCC
DEIEAIGAGDQGMMFGYATNETPELMPMPISLAHRLARRLAEVRKSRLLPYLRPDGKTQV
CHHHEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
TVEYDGDKPVRVDTIVVSTQHHPDVSQEEIRKDIIEHVVKPVIPEHLLDENTRYFVNPTG
EEEECCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCHHHHHCCCCEEEECCCC
RFVIGGPQGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAA
CEEEECCCCCCCCCCCEEEEECCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
GIADKIEIQLAYAIGVAKPVSIMVDTFGTGKIADEKIVELIKKHFDLRPAGIIRMLDLRR
CCCCEEEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCC
PIYKQTAAYGHFGRTDIDLPWERIDKAEILRMEAGL
HHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA