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The map label for this gene is leuD [H]

Identifier: 78043773

GI number: 78043773

Start: 455713

End: 456201

Strand: Direct

Name: leuD [H]

Synonym: CHY_0523

Alternate gene names: 78043773

Gene position: 455713-456201 (Clockwise)

Preceding gene: 78043190

Following gene: 78044738

Centisome position: 18.98

GC content: 44.17

Gene sequence:

>489_bases
ATGCGTGCTTTTAAATTTGGCGATGACATTGATACCGATGTAATTATTCCGGCAAGGTATCTTAATACTTCTGATCCTTT
GGAATTAGCCAAACACTGCATGGAGGATGCGGATCCGGAGTTTTCCAAAAAAGCTCGCCCCGGGGATGTGATCGTTGCCG
GGAAAAACTTTGGCTGCGGTAGTTCCCGGGAGCATGCTCCTATTGCGATCAAAGCTCTGGGGATCGAAGCGGTAATTGCC
AAATCCTTTGCCCGGATTTTTTACCGGAATAGTTTTAATATTGGGTTAAAAATTTTGGAATGTCCGGAAGCGGCGGAAAA
AATTCGCGATGGAGATGAACTTTCCATCAATTACGATACCGGAGAAATAAAAAATCTTACTACCGGCGAAAGCTACTTTG
CCAAACCCATACCGGAGTTTATGCAGAATATTATTGCCAAAGGAGGACTTATTAACTATGTCCGGGAGAAGCTGCAAGGG
ACCGTTTAA

Upstream 100 bases:

>100_bases
GATGGGGCATGTAGAATCGGAAGTTTACCTGGCGGGACCGGCGGTAGCAGCAGCTTCAGCCATTGCCGGAAAAATCGTTC
ATCCCGAGGAGGTGCGGTAA

Downstream 100 bases:

>100_bases
AATTTTGGTGCTGCCGGGGGATGGTATTGGACCGGAGATAATTAAGGAAGCGGTGAAGGTTTTAAAAGCGTTAGGGGAGA
AAAAGGGCATTACTTTTAAT

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MRAFKFGDDIDTDVIIPARYLNTSDPLELAKHCMEDADPEFSKKARPGDVIVAGKNFGCGSSREHAPIAIKALGIEAVIA
KSFARIFYRNSFNIGLKILECPEAAEKIRDGDELSINYDTGEIKNLTTGESYFAKPIPEFMQNIIAKGGLINYVREKLQG
TV

Sequences:

>Translated_162_residues
MRAFKFGDDIDTDVIIPARYLNTSDPLELAKHCMEDADPEFSKKARPGDVIVAGKNFGCGSSREHAPIAIKALGIEAVIA
KSFARIFYRNSFNIGLKILECPEAAEKIRDGDELSINYDTGEIKNLTTGESYFAKPIPEFMQNIIAKGGLINYVREKLQG
TV
>Mature_162_residues
MRAFKFGDDIDTDVIIPARYLNTSDPLELAKHCMEDADPEFSKKARPGDVIVAGKNFGCGSSREHAPIAIKALGIEAVIA
KSFARIFYRNSFNIGLKILECPEAAEKIRDGDELSINYDTGEIKNLTTGESYFAKPIPEFMQNIIAKGGLINYVREKLQG
TV

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=160, Percent_Identity=32.5, Blast_Score=74, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6320440, Length=172, Percent_Identity=38.953488372093, Blast_Score=103, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6321429, Length=103, Percent_Identity=40.7766990291262, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011824
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 17888; Mature: 17888

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAFKFGDDIDTDVIIPARYLNTSDPLELAKHCMEDADPEFSKKARPGDVIVAGKNFGCG
CCCCCCCCCCCCCEEECHHHCCCCCHHHHHHHHHHCCCCCHHHCCCCCCEEEECCCCCCC
SSREHAPIAIKALGIEAVIAKSFARIFYRNSFNIGLKILECPEAAEKIRDGDELSINYDT
CCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHCCCCCCEEEEECC
GEIKNLTTGESYFAKPIPEFMQNIIAKGGLINYVREKLQGTV
CCCCCCCCCCHHHHCHHHHHHHHHHHCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MRAFKFGDDIDTDVIIPARYLNTSDPLELAKHCMEDADPEFSKKARPGDVIVAGKNFGCG
CCCCCCCCCCCCCEEECHHHCCCCCHHHHHHHHHHCCCCCHHHCCCCCCEEEECCCCCCC
SSREHAPIAIKALGIEAVIAKSFARIFYRNSFNIGLKILECPEAAEKIRDGDELSINYDT
CCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHCCCCCCEEEEECC
GEIKNLTTGESYFAKPIPEFMQNIIAKGGLINYVREKLQGTV
CCCCCCCCCCHHHHCHHHHHHHHHHHCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]