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The map label for this gene is leuC [H]

Identifier: 78043190

GI number: 78043190

Start: 454447

End: 455712

Strand: Direct

Name: leuC [H]

Synonym: CHY_0522

Alternate gene names: 78043190

Gene position: 454447-455712 (Clockwise)

Preceding gene: 78044251

Following gene: 78043773

Centisome position: 18.92

GC content: 49.68

Gene sequence:

>1266_bases
ATGGGGATGACGATGACCGAGAAAATTCTTGCCTATCATGCCGGAAAAGAGCTTGTTGAACCGGGAGAATTAATTTCCTG
CAAGGTAGATGCGGTTTTAGCCAACGACATAACCGGACCGGTAGCGATAAGCGAGTTTAAAAAAATTGGAGTGGACTCGG
TTTTTGACCGGGACAGGGTTTATTTGGTGCCCGACCATTTTACCCCCAATAAAGACATTAAAAGTGCCGAACAGGCCAAA
ATTTTGCGGGATTTTGCTAAAGAGCAAAACCTAACCTATTATTTTGAAGTGGGTAAAATGGGAATTGAGCATGTGCTTCT
GCCGGAAGAAGGGTTGGTATTGCCGGGAGAGGTGATTATCGGTGCCGATTCCCATACCTGTACTTACGGGGGGCTGGGGG
CTTTTGCTACCGGGGTAGGTTCAACTGACCTTGCGGCGGCGATGGCCCTCGGGGAAACCTGGTTTAAAGTTCCGGAAAGC
ATTAAGTTTTACCTTTACGGGGAAAAGCTTTTACCTTACGTTTCCGCCAAAGATATCATTCTGTTTATAATCGGGCAGAT
TGGTGTTGACGGGGCGTTATATAAAGCGATGGAGTTCGCCGGTTCGGCTTTAAAGCTTATTTCGGTTGAAGGACGGTTAA
CCATGGCCAATATGGCTATAGAAGCGGGAGCAAAAAACGGCATCTTTCCGGTGGATGAGCTTACCCTGGAATATGTAAAA
GGCCGGGCAAAACGGGAGTTTAAGGTTTTTGCCAGCGATCCCGATGCCAGATACGAAGCGGTTTACGAAATTAACGTGGA
AGAAATCGAACCGCAGGTAGCTCTTCCCCATCTTCCGGAAAATGCCGTGCCGGTAAAGGAGGTATCCGGCAAGGACATTG
ATCAGGTGGTAATTGGTTCGTGCACCAACGGCCGAATTGAGGATTTACGGGTGGCGGCGGCGATCTTAAAAGGTAAAAAA
GTAAAGGATTATGTGCGGTGCATTATTATTCCCGGATCCCAGTCGGTTTACCGGCAGGCCCTTAAGGAAGGACTTATAGA
TATTTTCCTTGAAGCGGGGGCGGCGGTTTCCACTCCTACCTGTGGCCCCTGCCTGGGCGGCCACATGGGGATTTTAGCCA
AGGGAGAGAGAGCGGTTGCTACTACCAACCGCAATTTTGTGGGCAGGATGGGGCATGTAGAATCGGAAGTTTACCTGGCG
GGACCGGCGGTAGCAGCAGCTTCAGCCATTGCCGGAAAAATCGTTCATCCCGAGGAGGTGCGGTAA

Upstream 100 bases:

>100_bases
GCGGAGTTTCCACCGATATTTTAGAAGCATCGGCCAAGGCTTATTTAAATGGTATCAATAAATTACTTTTTGATTACCAG
GCGAAAGGGGAGAAGCAGTA

Downstream 100 bases:

>100_bases
ATGCGTGCTTTTAAATTTGGCGATGACATTGATACCGATGTAATTATTCCGGCAAGGTATCTTAATACTTCTGATCCTTT
GGAATTAGCCAAACACTGCA

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MGMTMTEKILAYHAGKELVEPGELISCKVDAVLANDITGPVAISEFKKIGVDSVFDRDRVYLVPDHFTPNKDIKSAEQAK
ILRDFAKEQNLTYYFEVGKMGIEHVLLPEEGLVLPGEVIIGADSHTCTYGGLGAFATGVGSTDLAAAMALGETWFKVPES
IKFYLYGEKLLPYVSAKDIILFIIGQIGVDGALYKAMEFAGSALKLISVEGRLTMANMAIEAGAKNGIFPVDELTLEYVK
GRAKREFKVFASDPDARYEAVYEINVEEIEPQVALPHLPENAVPVKEVSGKDIDQVVIGSCTNGRIEDLRVAAAILKGKK
VKDYVRCIIIPGSQSVYRQALKEGLIDIFLEAGAAVSTPTCGPCLGGHMGILAKGERAVATTNRNFVGRMGHVESEVYLA
GPAVAAASAIAGKIVHPEEVR

Sequences:

>Translated_421_residues
MGMTMTEKILAYHAGKELVEPGELISCKVDAVLANDITGPVAISEFKKIGVDSVFDRDRVYLVPDHFTPNKDIKSAEQAK
ILRDFAKEQNLTYYFEVGKMGIEHVLLPEEGLVLPGEVIIGADSHTCTYGGLGAFATGVGSTDLAAAMALGETWFKVPES
IKFYLYGEKLLPYVSAKDIILFIIGQIGVDGALYKAMEFAGSALKLISVEGRLTMANMAIEAGAKNGIFPVDELTLEYVK
GRAKREFKVFASDPDARYEAVYEINVEEIEPQVALPHLPENAVPVKEVSGKDIDQVVIGSCTNGRIEDLRVAAAILKGKK
VKDYVRCIIIPGSQSVYRQALKEGLIDIFLEAGAAVSTPTCGPCLGGHMGILAKGERAVATTNRNFVGRMGHVESEVYLA
GPAVAAASAIAGKIVHPEEVR
>Mature_420_residues
GMTMTEKILAYHAGKELVEPGELISCKVDAVLANDITGPVAISEFKKIGVDSVFDRDRVYLVPDHFTPNKDIKSAEQAKI
LRDFAKEQNLTYYFEVGKMGIEHVLLPEEGLVLPGEVIIGADSHTCTYGGLGAFATGVGSTDLAAAMALGETWFKVPESI
KFYLYGEKLLPYVSAKDIILFIIGQIGVDGALYKAMEFAGSALKLISVEGRLTMANMAIEAGAKNGIFPVDELTLEYVKG
RAKREFKVFASDPDARYEAVYEINVEEIEPQVALPHLPENAVPVKEVSGKDIDQVVIGSCTNGRIEDLRVAAAILKGKKV
KDYVRCIIIPGSQSVYRQALKEGLIDIFLEAGAAVSTPTCGPCLGGHMGILAKGERAVATTNRNFVGRMGHVESEVYLAG
PAVAAASAIAGKIVHPEEVR

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=456, Percent_Identity=30.4824561403509, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI41352693, Length=403, Percent_Identity=27.7915632754342, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI8659555, Length=387, Percent_Identity=26.0981912144703, Blast_Score=105, Evalue=8e-23,
Organism=Escherichia coli, GI1786259, Length=466, Percent_Identity=35.6223175965665, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI87081781, Length=334, Percent_Identity=27.5449101796407, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI2367097, Length=460, Percent_Identity=26.5217391304348, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1787531, Length=360, Percent_Identity=26.3888888888889, Blast_Score=95, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI25149337, Length=448, Percent_Identity=29.9107142857143, Blast_Score=140, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI32564738, Length=344, Percent_Identity=31.9767441860465, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI25149342, Length=285, Percent_Identity=31.2280701754386, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17568399, Length=382, Percent_Identity=24.869109947644, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320440, Length=453, Percent_Identity=34.2163355408389, Blast_Score=219, Evalue=8e-58,
Organism=Saccharomyces cerevisiae, GI6321429, Length=434, Percent_Identity=35.0230414746544, Blast_Score=217, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6323335, Length=448, Percent_Identity=31.6964285714286, Blast_Score=176, Evalue=7e-45,
Organism=Saccharomyces cerevisiae, GI6322261, Length=463, Percent_Identity=28.0777537796976, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI281365315, Length=449, Percent_Identity=30.0668151447661, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI17864292, Length=449, Percent_Identity=30.0668151447661, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI28571643, Length=448, Percent_Identity=30.1339285714286, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI161076999, Length=412, Percent_Identity=30.5825242718447, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI17137564, Length=377, Percent_Identity=25.7294429708223, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24645686, Length=379, Percent_Identity=26.3852242744063, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- InterPro:   IPR011823 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 45294; Mature: 45163

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMTMTEKILAYHAGKELVEPGELISCKVDAVLANDITGPVAISEFKKIGVDSVFDRDRV
CCCCHHHHHHHHHCCHHHCCCCCEEEEEEEEEEECCCCCCHHHHHHHHCCCCHHCCCCCE
YLVPDHFTPNKDIKSAEQAKILRDFAKEQNLTYYFEVGKMGIEHVLLPEEGLVLPGEVII
EEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCEECCCCEEE
GADSHTCTYGGLGAFATGVGSTDLAAAMALGETWFKVPESIKFYLYGEKLLPYVSAKDII
ECCCCEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHCCCCCCCCEE
LFIIGQIGVDGALYKAMEFAGSALKLISVEGRLTMANMAIEAGAKNGIFPVDELTLEYVK
EEEEEHHCCCHHHHHHHHHCCCEEEEEEECCCEEEEEEEEECCCCCCCCCHHHHHHHHHH
GRAKREFKVFASDPDARYEAVYEINVEEIEPQVALPHLPENAVPVKEVSGKDIDQVVIGS
CCCCCEEEEEECCCCCCEEEEEEEEHHHCCCCEECCCCCCCCCCHHHCCCCCCCEEEEEC
CTNGRIEDLRVAAAILKGKKVKDYVRCIIIPGSQSVYRQALKEGLIDIFLEAGAAVSTPT
CCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
CGPCLGGHMGILAKGERAVATTNRNFVGRMGHVESEVYLAGPAVAAASAIAGKIVHPEEV
CCCCCCCCCCEEECCCEEEEECCCCHHCCCCCCCCEEEEECCHHHHHHHHHCCCCCHHCC
R
C
>Mature Secondary Structure 
GMTMTEKILAYHAGKELVEPGELISCKVDAVLANDITGPVAISEFKKIGVDSVFDRDRV
CCCHHHHHHHHHCCHHHCCCCCEEEEEEEEEEECCCCCCHHHHHHHHCCCCHHCCCCCE
YLVPDHFTPNKDIKSAEQAKILRDFAKEQNLTYYFEVGKMGIEHVLLPEEGLVLPGEVII
EEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCEECCCCEEE
GADSHTCTYGGLGAFATGVGSTDLAAAMALGETWFKVPESIKFYLYGEKLLPYVSAKDII
ECCCCEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHCCCCCCCCEE
LFIIGQIGVDGALYKAMEFAGSALKLISVEGRLTMANMAIEAGAKNGIFPVDELTLEYVK
EEEEEHHCCCHHHHHHHHHCCCEEEEEEECCCEEEEEEEEECCCCCCCCCHHHHHHHHHH
GRAKREFKVFASDPDARYEAVYEINVEEIEPQVALPHLPENAVPVKEVSGKDIDQVVIGS
CCCCCEEEEEECCCCCCEEEEEEEEHHHCCCCEECCCCCCCCCCHHHCCCCCCCEEEEEC
CTNGRIEDLRVAAAILKGKKVKDYVRCIIIPGSQSVYRQALKEGLIDIFLEAGAAVSTPT
CCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
CGPCLGGHMGILAKGERAVATTNRNFVGRMGHVESEVYLAGPAVAAASAIAGKIVHPEEV
CCCCCCCCCCEEECCCEEEEECCCCHHCCCCCCCCEEEEECCHHHHHHHHHCCCCCHHCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA