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The map label for this gene is asrB [H]

Identifier: 78043750

GI number: 78043750

Start: 824909

End: 825751

Strand: Direct

Name: asrB [H]

Synonym: CHY_0934

Alternate gene names: 78043750

Gene position: 824909-825751 (Clockwise)

Preceding gene: 78043358

Following gene: 78042982

Centisome position: 34.35

GC content: 45.91

Gene sequence:

>843_bases
ATGGCACTGGAAAATCCTTTATTACCGTATATTGCAACCATAACCAAGGTGATTGATGAAACCCCCGATGTGAAAACTTT
TCAAATGGTTTTTGACGATCCCGGGGTATTTGAAGTTTTTAAACAAAAACCAGGACAAGTTGCGCAGATATCCATTTTTG
GGGTGGGGGAAGCCACCATTTCTATAACCTCTTCGCCAACCAGGACGGGAATGCTCGAGTTTTCCATCAAGAAGGTGGGA
AGACTTACTTCCGTTATCCACCAGATGGAGCCAGGAATGAAGGTGGGAATCCGGGGTCCCTACGGCAATCATTTTCCCTA
TGAAATGATGAAAGGAAAGGATCTCCTTTTTATCGGCGGAGGTATTGGTTTAGCGCCGCTAAGATCGCTTATTGACTTTG
TTTTAGCCCCGGAAAACCGCAAGGATTACGGTAAGGTGGAAATCCTTTACGGAGCCCGTTCCTCCGCCGACCTTTGCTTT
AAATACGATTTATTTGACAACTGGCCCAAGCAGCCGGACACCAAGGTTTACGTTACCATCGACCGTCCCGAAGAAGGCTG
GGATGGCCATGTGGGATTTGTTCCTGCTTACCTGGAAGAACTGAATCCCAATCCTCAAAATAAAGTAACCATTACCTGCG
GACCTCCGATCATGATTAAGTTTGTCTTGCAGGCGTTGGAGAAGATGGGTTATTCCGAGGACCAGGTGGTAACCACTTTG
GAATTAAAAATGAAATGCGGTATTGGGAAATGCGGACGCTGCAATATCGGAAGCAAATTTGTATGCGTTGATGGTCCCGT
CTTTACTTTGCGTGACCTTAAAAAACTGCCTCCGGAGTTTTAA

Upstream 100 bases:

>100_bases
ATAGCATAGTGGGGTGCGTAGGCTGTGGCCGTTGCGTCCAGCGTTGCCCCGAAAATATCGACATCCGGGCAATAATTTCC
GACATAAAGGGTGGGGTGTA

Downstream 100 bases:

>100_bases
AAAATTACCCGGCGTATGCCGGGTTTTTATTTTGTAGAAATAGCGTGAATTGTGTAGAAATATTTTGATAAATATTCTGG
GCGAAGGGAAAGGAATATTA

Product: hydrogenase subunit gamma

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MALENPLLPYIATITKVIDETPDVKTFQMVFDDPGVFEVFKQKPGQVAQISIFGVGEATISITSSPTRTGMLEFSIKKVG
RLTSVIHQMEPGMKVGIRGPYGNHFPYEMMKGKDLLFIGGGIGLAPLRSLIDFVLAPENRKDYGKVEILYGARSSADLCF
KYDLFDNWPKQPDTKVYVTIDRPEEGWDGHVGFVPAYLEELNPNPQNKVTITCGPPIMIKFVLQALEKMGYSEDQVVTTL
ELKMKCGIGKCGRCNIGSKFVCVDGPVFTLRDLKKLPPEF

Sequences:

>Translated_280_residues
MALENPLLPYIATITKVIDETPDVKTFQMVFDDPGVFEVFKQKPGQVAQISIFGVGEATISITSSPTRTGMLEFSIKKVG
RLTSVIHQMEPGMKVGIRGPYGNHFPYEMMKGKDLLFIGGGIGLAPLRSLIDFVLAPENRKDYGKVEILYGARSSADLCF
KYDLFDNWPKQPDTKVYVTIDRPEEGWDGHVGFVPAYLEELNPNPQNKVTITCGPPIMIKFVLQALEKMGYSEDQVVTTL
ELKMKCGIGKCGRCNIGSKFVCVDGPVFTLRDLKKLPPEF
>Mature_279_residues
ALENPLLPYIATITKVIDETPDVKTFQMVFDDPGVFEVFKQKPGQVAQISIFGVGEATISITSSPTRTGMLEFSIKKVGR
LTSVIHQMEPGMKVGIRGPYGNHFPYEMMKGKDLLFIGGGIGLAPLRSLIDFVLAPENRKDYGKVEILYGARSSADLCFK
YDLFDNWPKQPDTKVYVTIDRPEEGWDGHVGFVPAYLEELNPNPQNKVTITCGPPIMIKFVLQALEKMGYSEDQVVTTLE
LKMKCGIGKCGRCNIGSKFVCVDGPVFTLRDLKKLPPEF

Specific function: This enzyme catalyzes the hydrogen sulfide production from sulfite. It is strictly anaerobic. It is regulated by electron acceptors rather than by cysteine [H]

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Homo sapiens, GI47778923, Length=178, Percent_Identity=27.5280898876405, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323510, Length=176, Percent_Identity=28.9772727272727, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323552, Length=175, Percent_Identity=28, Blast_Score=66, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24662938, Length=174, Percent_Identity=30.4597701149425, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI281366101, Length=174, Percent_Identity=30.4597701149425, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24662934, Length=174, Percent_Identity=30.4597701149425, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI116007804, Length=175, Percent_Identity=26.8571428571429, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI116007806, Length=175, Percent_Identity=26.8571428571429, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012165
- InterPro:   IPR019480
- InterPro:   IPR017927
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938
- InterPro:   IPR014260 [H]

Pfam domain/function: PF10418 DHODB_Fe-S_bind; PF00970 FAD_binding_6; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 31122; Mature: 30991

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALENPLLPYIATITKVIDETPDVKTFQMVFDDPGVFEVFKQKPGQVAQISIFGVGEATI
CCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHCCCCCEEEEEEEEECCEEE
SITSSPTRTGMLEFSIKKVGRLTSVIHQMEPGMKVGIRGPYGNHFPYEMMKGKDLLFIGG
EEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCCEEEEECC
GIGLAPLRSLIDFVLAPENRKDYGKVEILYGARSSADLCFKYDLFDNWPKQPDTKVYVTI
CCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCEEEEEE
DRPEEGWDGHVGFVPAYLEELNPNPQNKVTITCGPPIMIKFVLQALEKMGYSEDQVVTTL
ECCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEE
ELKMKCGIGKCGRCNIGSKFVCVDGPVFTLRDLKKLPPEF
EEEEECCCCCCCCCCCCCEEEEECCCEEHHHHHHHCCCCC
>Mature Secondary Structure 
ALENPLLPYIATITKVIDETPDVKTFQMVFDDPGVFEVFKQKPGQVAQISIFGVGEATI
CCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHCCCCCEEEEEEEEECCEEE
SITSSPTRTGMLEFSIKKVGRLTSVIHQMEPGMKVGIRGPYGNHFPYEMMKGKDLLFIGG
EEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCCEEEEECC
GIGLAPLRSLIDFVLAPENRKDYGKVEILYGARSSADLCFKYDLFDNWPKQPDTKVYVTI
CCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCEEEEEE
DRPEEGWDGHVGFVPAYLEELNPNPQNKVTITCGPPIMIKFVLQALEKMGYSEDQVVTTL
ECCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEE
ELKMKCGIGKCGRCNIGSKFVCVDGPVFTLRDLKKLPPEF
EEEEECCCCCCCCCCCCCEEEEECCCEEHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1704886; 11677609 [H]