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The map label for this gene is cysK [H]

Identifier: 78043721

GI number: 78043721

Start: 716551

End: 717471

Strand: Direct

Name: cysK [H]

Synonym: CHY_0808

Alternate gene names: 78043721

Gene position: 716551-717471 (Clockwise)

Preceding gene: 78044694

Following gene: 78043483

Centisome position: 29.84

GC content: 46.8

Gene sequence:

>921_bases
ATGCGAATTTATGAGGATATTACCCAGCTTATCGGCAAGACCCCGTTATTGAAATTAAAAAGAGTTACCAGAGGTGTGAA
AGCAACAGTTGTGGCCAAGCTTGAGTATTTTAACCCGGGAGGTAGTGTTAAGGACCGTATCGCGTTTAACATGATTAAAG
CTGCTGAAGAACAGGGGCTTTTAGATAAGGATACCGTCATTATTGAGCCTACTTCTGGCAATACCGGGATTGGCCTTGCG
ATGGTGGCCGCAGCCCGGGGTTATCGTTTAATTATTACCATGCCCGAAACCATGAGTGTGGAGCGGCGAATGCTTTTAAA
AGCATACGGGGCGGAAATAGTTCTGACTCCCGGAGAGCTTGGGATGACCGGTGCGGTAAACAAAGCTCTGGAACTGGCTA
AAGAATTTCCCAAAGCTTTTATTCCGCAACAGTTTGAAAACCCGGCTAATCCGGAAATCCACCGGAAAACAACGGCAATA
GAAATCTGGGAAGATACTGATGGTCAGGTGGATATTGTGGTTGGTGGTGTTGGGACCGGAGGTACCATTACCGGAGTAGG
AGAGGTTCTTAAAGCCAAAAAACCGGAAGTAAAAATTATAGCGGTGGAGCCGGCAGATTCACCGGTATTATCGGGAGGAA
AACCCGGCCCTCATAAAATTCAGGGAATTGGTGCGGGTTTTGTACCTAAAGTATTTAACAGGGACGTGGTGGATGAGATT
ATTCAGGTAGAAAGTGATGATGCTTTTGCTATGGCTAAAAAAATTGCAAAAGAAGAAGGGGTACTGGTGGGGATTTCTTC
CGGTGCGGCTCTCTGGGCAGCGTTAGAAGTGGCCAAAAGACCGGAAAATGAAGGGAAACTTATCGTGGTTGTCTTACCCG
ATACCGGCGAACGTTACCTGTCCACACCCCTGTTTAGCTAA

Upstream 100 bases:

>100_bases
GCTCCGGTTTTTTTATGGATTAAAAACATAATAGTTGACAGGATTAGTAGGAATTAATATAATGGGTTTAGAAGGACAAT
ACTAAAATGAGGTGATTTTA

Downstream 100 bases:

>100_bases
CCCTCAAGCTTTCAAAGAGGGGGCTCTAAGATGGAGTGTGATATCATTACTACTGATTACGGTTATGTAGCCATTGCAGT
TTCGGAGAAGGGGGTTTATG

Product: cysteine synthase A

Products: NA

Alternate protein names: OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MRIYEDITQLIGKTPLLKLKRVTRGVKATVVAKLEYFNPGGSVKDRIAFNMIKAAEEQGLLDKDTVIIEPTSGNTGIGLA
MVAAARGYRLIITMPETMSVERRMLLKAYGAEIVLTPGELGMTGAVNKALELAKEFPKAFIPQQFENPANPEIHRKTTAI
EIWEDTDGQVDIVVGGVGTGGTITGVGEVLKAKKPEVKIIAVEPADSPVLSGGKPGPHKIQGIGAGFVPKVFNRDVVDEI
IQVESDDAFAMAKKIAKEEGVLVGISSGAALWAALEVAKRPENEGKLIVVVLPDTGERYLSTPLFS

Sequences:

>Translated_306_residues
MRIYEDITQLIGKTPLLKLKRVTRGVKATVVAKLEYFNPGGSVKDRIAFNMIKAAEEQGLLDKDTVIIEPTSGNTGIGLA
MVAAARGYRLIITMPETMSVERRMLLKAYGAEIVLTPGELGMTGAVNKALELAKEFPKAFIPQQFENPANPEIHRKTTAI
EIWEDTDGQVDIVVGGVGTGGTITGVGEVLKAKKPEVKIIAVEPADSPVLSGGKPGPHKIQGIGAGFVPKVFNRDVVDEI
IQVESDDAFAMAKKIAKEEGVLVGISSGAALWAALEVAKRPENEGKLIVVVLPDTGERYLSTPLFS
>Mature_306_residues
MRIYEDITQLIGKTPLLKLKRVTRGVKATVVAKLEYFNPGGSVKDRIAFNMIKAAEEQGLLDKDTVIIEPTSGNTGIGLA
MVAAARGYRLIITMPETMSVERRMLLKAYGAEIVLTPGELGMTGAVNKALELAKEFPKAFIPQQFENPANPEIHRKTTAI
EIWEDTDGQVDIVVGGVGTGGTITGVGEVLKAKKPEVKIIAVEPADSPVLSGGKPGPHKIQGIGAGFVPKVFNRDVVDEI
IQVESDDAFAMAKKIAKEEGVLVGISSGAALWAALEVAKRPENEGKLIVVVLPDTGERYLSTPLFS

Specific function: Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=310, Percent_Identity=42.9032258064516, Blast_Score=241, Evalue=6e-64,
Organism=Homo sapiens, GI295821200, Length=310, Percent_Identity=42.9032258064516, Blast_Score=241, Evalue=6e-64,
Organism=Homo sapiens, GI4557415, Length=310, Percent_Identity=42.9032258064516, Blast_Score=241, Evalue=6e-64,
Organism=Homo sapiens, GI11345492, Length=285, Percent_Identity=26.3157894736842, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1788754, Length=314, Percent_Identity=54.4585987261147, Blast_Score=298, Evalue=2e-82,
Organism=Escherichia coli, GI2367138, Length=299, Percent_Identity=42.1404682274247, Blast_Score=229, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17535051, Length=299, Percent_Identity=55.8528428093645, Blast_Score=335, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI115535073, Length=305, Percent_Identity=54.4262295081967, Blast_Score=307, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI17562970, Length=303, Percent_Identity=50.1650165016502, Blast_Score=304, Evalue=5e-83,
Organism=Caenorhabditis elegans, GI32566674, Length=297, Percent_Identity=50.5050505050505, Blast_Score=298, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI17561720, Length=303, Percent_Identity=48.5148514851485, Blast_Score=267, Evalue=6e-72,
Organism=Caenorhabditis elegans, GI32566672, Length=213, Percent_Identity=52.5821596244132, Blast_Score=227, Evalue=5e-60,
Organism=Caenorhabditis elegans, GI17534315, Length=316, Percent_Identity=41.7721518987342, Blast_Score=204, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI25147552, Length=312, Percent_Identity=38.4615384615385, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17561716, Length=117, Percent_Identity=50.4273504273504, Blast_Score=117, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6321594, Length=315, Percent_Identity=40.3174603174603, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6321449, Length=332, Percent_Identity=35.2409638554217, Blast_Score=150, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24643623, Length=310, Percent_Identity=41.9354838709677, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI20129101, Length=310, Percent_Identity=41.9354838709677, Blast_Score=219, Evalue=2e-57,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 32727; Mature: 32727

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIYEDITQLIGKTPLLKLKRVTRGVKATVVAKLEYFNPGGSVKDRIAFNMIKAAEEQGL
CCHHHHHHHHHCCCCHHHHHHHHCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCC
LDKDTVIIEPTSGNTGIGLAMVAAARGYRLIITMPETMSVERRMLLKAYGAEIVLTPGEL
CCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEECCCC
GMTGAVNKALELAKEFPKAFIPQQFENPANPEIHRKTTAIEIWEDTDGQVDIVVGGVGTG
CCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCEEEEEEEEECCCCEEEEEEECCCCC
GTITGVGEVLKAKKPEVKIIAVEPADSPVLSGGKPGPHKIQGIGAGFVPKVFNRDVVDEI
CCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCHHHHCHHHHHHH
IQVESDDAFAMAKKIAKEEGVLVGISSGAALWAALEVAKRPENEGKLIVVVLPDTGERYL
HHCCCCCHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHH
STPLFS
CCCCCC
>Mature Secondary Structure
MRIYEDITQLIGKTPLLKLKRVTRGVKATVVAKLEYFNPGGSVKDRIAFNMIKAAEEQGL
CCHHHHHHHHHCCCCHHHHHHHHCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCC
LDKDTVIIEPTSGNTGIGLAMVAAARGYRLIITMPETMSVERRMLLKAYGAEIVLTPGEL
CCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEECCCC
GMTGAVNKALELAKEFPKAFIPQQFENPANPEIHRKTTAIEIWEDTDGQVDIVVGGVGTG
CCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCEEEEEEEEECCCCEEEEEEECCCCC
GTITGVGEVLKAKKPEVKIIAVEPADSPVLSGGKPGPHKIQGIGAGFVPKVFNRDVVDEI
CCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCHHHHCHHHHHHH
IQVESDDAFAMAKKIAKEEGVLVGISSGAALWAALEVAKRPENEGKLIVVVLPDTGERYL
HHCCCCCHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHH
STPLFS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]