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The map label for this gene is ogt [H]

Identifier: 78043483

GI number: 78043483

Start: 717502

End: 717993

Strand: Direct

Name: ogt [H]

Synonym: CHY_0809

Alternate gene names: 78043483

Gene position: 717502-717993 (Clockwise)

Preceding gene: 78043721

Following gene: 78043638

Centisome position: 29.88

GC content: 47.76

Gene sequence:

>492_bases
ATGGAGTGTGATATCATTACTACTGATTACGGTTATGTAGCCATTGCAGTTTCGGAGAAGGGGGTTTATGCCGTTTCCCT
TCCTAAAGCTACTCCGAAAGCTGCCTTAGAGGAAATCGGCGTAGAGGTAAATCCTGTAAAATCCGAATTATTTTGTCGAA
CTGCCGAATTATTAAAGCGCTATTATGCCGGGGAGAAAGTGGATTTTTCTTCCTTGCCCCTTGATATAAGCTGGGCTTCG
CCTTTTGCCCGCAAAGTCTATGAAAAGCTTCGAAAAATTCCCTACGGGGCAACCGTTTCCTATGGTGAATTAGCAGGTCT
TTCGGGAAACCCGAAAGCGGCCCGGGCGGTAGGACGGGCCATGGCCACCAACAAAATTCCGGTAATAATCCCCTGACATC
GAGTCCTGGCTGCCGGCGGCGGCCTGGGAGGATTTGCCGGGGGACTATCTTTAAAAGAAAAAATGCTTAAAATGGAAAAG
CAGGCGTTATAA

Upstream 100 bases:

>100_bases
AGGGAAACTTATCGTGGTTGTCTTACCCGATACCGGCGAACGTTACCTGTCCACACCCCTGTTTAGCTAACCCTCAAGCT
TTCAAAGAGGGGGCTCTAAG

Downstream 100 bases:

>100_bases
CCTGCTTTTTATCTTTCTTTTTCTAAATTTAGCTGGTTATATTTTTGCAAGTCGGCAACTCTAATTTTGTAATCAACAAT
AGTGGGCTTAAAAATGGGGA

Product: methylated-DNA-protein-cysteine methyltransferase, selenocysteine-containing

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MECDIITTDYGYVAIAVSEKGVYAVSLPKATPKAALEEIGVEVNPVKSELFCRTAELLKRYYAGEKVDFSSLPLDISWAS
PFARKVYEKLRKIPYGATVSYGELAGLSGNPKAARAVGRAMATNKIPVIIPUHRVLAAGGGLGGFAGGLSLKEKMLKMEK
QAL

Sequences:

>Translated_163_residues
MECDIITTDYGYVAIAVSEKGVYAVSLPKATPKAALEEIGVEVNPVKSELFCRTAELLKRYYAGEKVDFSSLPLDISWAS
PFARKVYEKLRKIPYGATVSYGELAGLSGNPKAARAVGRAMATNKIPVIIP*HRVLAAGGGLGGFAGGLSLKEKMLKMEK
QAL
>Mature_163_residues
MECDIITTDYGYVAIAVSEKGVYAVSLPKATPKAALEEIGVEVNPVKSELFCRTAELLKRYYAGEKVDFSSLPLDISWAS
PFARKVYEKLRKIPYGATVSYGELAGLSGNPKAARAVGRAMATNKIPVIIP*HRVLAAGGGLGGFAGGLSLKEKMLKMEK
QAL

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=83, Percent_Identity=49.3975903614458, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1788542, Length=155, Percent_Identity=35.4838709677419, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1787596, Length=105, Percent_Identity=40.9523809523809, Blast_Score=82, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=41.25, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=41.25, Blast_Score=66, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6320001, Length=91, Percent_Identity=35.1648351648352, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17319; Mature: 17319

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MECDIITTDYGYVAIAVSEKGVYAVSLPKATPKAALEEIGVEVNPVKSELFCRTAELLKR
CCCCEEEECCCEEEEEEECCCEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
YYAGEKVDFSSLPLDISWASPFARKVYEKLRKIPYGATVSYGELAGLSGNPKAARAVGRA
HHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCHHHHHHHHH
MATNKIPVIIPHRVLAAGGGLGGFAGGLSLKEKMLKMEKQAL
HHCCCCCEEECCHHHHCCCCCCCHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MECDIITTDYGYVAIAVSEKGVYAVSLPKATPKAALEEIGVEVNPVKSELFCRTAELLKR
CCCCEEEECCCEEEEEEECCCEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
YYAGEKVDFSSLPLDISWASPFARKVYEKLRKIPYGATVSYGELAGLSGNPKAARAVGRA
HHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCHHHHHHHHH
MATNKIPVIIPHRVLAAGGGLGGFAGGLSLKEKMLKMEKQAL
HHCCCCCEEECCHHHHCCCCCCCHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]