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The map label for this gene is pgaB [H]

Identifier: 78043691

GI number: 78043691

Start: 1657467

End: 1658393

Strand: Direct

Name: pgaB [H]

Synonym: CHY_1851

Alternate gene names: 78043691

Gene position: 1657467-1658393 (Clockwise)

Preceding gene: 78043097

Following gene: 78043621

Centisome position: 69.02

GC content: 35.71

Gene sequence:

>927_bases
ATGTTAAGAAAAGTTTTTGCCGCATTTTTGCTTTTCCTTTTTATTTTTATCTTTTCCGGATGCACGCCTTCTTCCTCTTC
TTCCAGTATTCTGGTCCTGGAATATCACGGAGTAGTTCCAGATAATGCCTGGGGTAAAGTTAATCATCTTTACAATATCA
GGGTTGAAACCTTTGAAAACCAGATAAAAACTTTATTAAATGCCGGCTACAAACCCATCTCTGCCGAAAAGCTTATTGAC
TTTTACTATAACGGCTATAAACCATCCGGGAAAGAATTTTTAATAACCTTTGATGACGGCTACCGCAACAATTACCTGTA
TGCTTTCCCTATTCTAAAAAAATACCGGATTCCGGCCGAAATTAACTTAATTGTTGCCCGTATTGACCAGGCAAAAAACC
ATAACGATCCCATAAAAGGCTATCTAAACTGGGCTGAAGTTCGAGAAATGGCCAAGTCCGGTTTAATCTATTTTGGCTCT
CATACCTACGATAGCCATAATAAAGTTATTACCGGTAAAAATAAAAAGAAAAATTATCCCTTTTTAGGACCAATTTATCT
TGCAAATCAAAAAAGAATGGAAACATGGCAGGAAGCTGAAAAAAGGGTAAATAAAGACTTAATGCTCTCCGTTGAGCACA
TTTCATATGAAACAGGAATTAGACCAGAAATCTTTTGCTATCCCCACGGCAACTATAATACCTGGTTTATAAGTCGTCTT
AAAGAAAATGGATTTAAAATAGCTTTAGCTGGCAATATTCCGCAAAATAATAACCCCTTTACCGTAAAGCGTATTTTGGT
AACCGATTCCCTGCGCGGAAAAATACTTCTAATGAAATTAAATCTTGTAAAATACTCTAATATTCTGGATTTTATAATTA
AAAAGGCTTCTGGTTCTGCCCAGAAGCCTGATTTTTCAAAAACTTAA

Upstream 100 bases:

>100_bases
CTTCAGATGTAATTGGCGGAATGTTATTAGGAACATTTGTGAACTTTTCAAGGCTCCTTTTAGAGCAATATTATCTATCT
GGCAAAAGAAGGGATATCAA

Downstream 100 bases:

>100_bases
ACCTTGATTATTTCCACTCTGGTATCCAATTCACTGGCTCCGCCGCCAATAGGATCGGACAAACAAGCATTTTTCAAGGC
CACATCCACTAATTGAACAT

Product: polysaccharide deacetylase family protein

Products: NA

Alternate protein names: PGA N-deacetylase; Poly-beta-1,6-GlcNAc N-deacetylase [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MLRKVFAAFLLFLFIFIFSGCTPSSSSSSILVLEYHGVVPDNAWGKVNHLYNIRVETFENQIKTLLNAGYKPISAEKLID
FYYNGYKPSGKEFLITFDDGYRNNYLYAFPILKKYRIPAEINLIVARIDQAKNHNDPIKGYLNWAEVREMAKSGLIYFGS
HTYDSHNKVITGKNKKKNYPFLGPIYLANQKRMETWQEAEKRVNKDLMLSVEHISYETGIRPEIFCYPHGNYNTWFISRL
KENGFKIALAGNIPQNNNPFTVKRILVTDSLRGKILLMKLNLVKYSNILDFIIKKASGSAQKPDFSKT

Sequences:

>Translated_308_residues
MLRKVFAAFLLFLFIFIFSGCTPSSSSSSILVLEYHGVVPDNAWGKVNHLYNIRVETFENQIKTLLNAGYKPISAEKLID
FYYNGYKPSGKEFLITFDDGYRNNYLYAFPILKKYRIPAEINLIVARIDQAKNHNDPIKGYLNWAEVREMAKSGLIYFGS
HTYDSHNKVITGKNKKKNYPFLGPIYLANQKRMETWQEAEKRVNKDLMLSVEHISYETGIRPEIFCYPHGNYNTWFISRL
KENGFKIALAGNIPQNNNPFTVKRILVTDSLRGKILLMKLNLVKYSNILDFIIKKASGSAQKPDFSKT
>Mature_308_residues
MLRKVFAAFLLFLFIFIFSGCTPSSSSSSILVLEYHGVVPDNAWGKVNHLYNIRVETFENQIKTLLNAGYKPISAEKLID
FYYNGYKPSGKEFLITFDDGYRNNYLYAFPILKKYRIPAEINLIVARIDQAKNHNDPIKGYLNWAEVREMAKSGLIYFGS
HTYDSHNKVITGKNKKKNYPFLGPIYLANQKRMETWQEAEKRVNKDLMLSVEHISYETGIRPEIFCYPHGNYNTWFISRL
KENGFKIALAGNIPQNNNPFTVKRILVTDSLRGKILLMKLNLVKYSNILDFIIKKASGSAQKPDFSKT

Specific function: Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl- D-glucosamine (PGA), a biofilm adhesin polysaccharide. N- deacetylation promotes PGA export through the PgaA porin [H]

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Escherichia coli, GI1787260, Length=294, Percent_Identity=22.7891156462585, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1786322, Length=247, Percent_Identity=27.1255060728745, Blast_Score=71, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: NA

Molecular weight: Translated: 35528; Mature: 35528

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRKVFAAFLLFLFIFIFSGCTPSSSSSSILVLEYHGVVPDNAWGKVNHLYNIRVETFEN
CHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEEEEHHHHH
QIKTLLNAGYKPISAEKLIDFYYNGYKPSGKEFLITFDDGYRNNYLYAFPILKKYRIPAE
HHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEEECHHHHCCCCC
INLIVARIDQAKNHNDPIKGYLNWAEVREMAKSGLIYFGSHTYDSHNKVITGKNKKKNYP
EEEEEEEEHHCCCCCCCHHHHCCHHHHHHHHHCCEEEEECCEECCCCCEEECCCCCCCCC
FLGPIYLANQKRMETWQEAEKRVNKDLMLSVEHISYETGIRPEIFCYPHGNYNTWFISRL
EECEEEECCCHHHHHHHHHHHHCCHHHEEEEEHCCCCCCCCCEEEEEECCCCCEEEEEEE
KENGFKIALAGNIPQNNNPFTVKRILVTDSLRGKILLMKLNLVKYSNILDFIIKKASGSA
CCCCEEEEEECCCCCCCCCEEEEEEEEECCCCCEEEEEEEEHHHHHHHHHHHHHHCCCCC
QKPDFSKT
CCCCCCCC
>Mature Secondary Structure
MLRKVFAAFLLFLFIFIFSGCTPSSSSSSILVLEYHGVVPDNAWGKVNHLYNIRVETFEN
CHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEEEEHHHHH
QIKTLLNAGYKPISAEKLIDFYYNGYKPSGKEFLITFDDGYRNNYLYAFPILKKYRIPAE
HHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEEECHHHHCCCCC
INLIVARIDQAKNHNDPIKGYLNWAEVREMAKSGLIYFGSHTYDSHNKVITGKNKKKNYP
EEEEEEEEHHCCCCCCCHHHHCCHHHHHHHHHCCEEEEECCEECCCCCEEECCCCCCCCC
FLGPIYLANQKRMETWQEAEKRVNKDLMLSVEHISYETGIRPEIFCYPHGNYNTWFISRL
EECEEEECCCHHHHHHHHHHHHCCHHHEEEEEHCCCCCCCCCEEEEEECCCCCEEEEEEE
KENGFKIALAGNIPQNNNPFTVKRILVTDSLRGKILLMKLNLVKYSNILDFIIKKASGSA
CCCCEEEEEECCCCCCCCCEEEEEEEEECCCCCEEEEEEEEHHHHHHHHHHHHHHCCCCC
QKPDFSKT
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]