Click here to switch to the map view.

The map label for this gene is grdE [H]

Identifier: 78043612

GI number: 78043612

Start: 2139989

End: 2141275

Strand: Direct

Name: grdE [H]

Synonym: CHY_2391

Alternate gene names: 78043612

Gene position: 2139989-2141275 (Clockwise)

Preceding gene: 78043049

Following gene: 78044558

Centisome position: 89.11

GC content: 47.94

Gene sequence:

>1287_bases
ATGCGGCTTACCCTTGGGAAAATTAACATTAAAGGGCTGGAATTTGGCCCCAGTACCCGGGTCGAAAACGGAGTTCTTTA
CGTTAATAAAGACGAATTAACAGCCTTATTGAAAGAGGATAACCGGATTAGCTCCGTTGAGGTTTTCTTAGCCCGCCCGG
GCGAATCGGTACGAATTGTACCGGTAAAAGACGTCATTGAACCCCGGGTGAAGGTGGAAGGCCCAGGAAATATTTTCCCC
GGTTTTCTCGGTAAAGTAGAAATGGTGGGCAGCGGCCGGACCCACGTTTTAAAAGGTGCTGCTGTGGTCACTACCGGAAA
GATTGTAGGATTCCAGGAAGGAATTATTGACATGACCGGTCCTGGAGCCGAGTATACTCCTTTTTCCAAAACCTATAACG
TTGTAGTTAAAATCGAACCGGTAGATGGATTAAGTCAACATGAACACGAAAGTGTGGTCCGGTTAGCCGGACTTAAAGCA
GCGGCCTACCTTGGGGAAGCTGGTAAAAACATTACCCCCGATGAAGAGGTAACTTATGAAGTTGCTCCGCTCTTAGAAGC
CGCTTCAAAGTACCCCGGACTTCCAAAAGTAGCTTATGTTTACATGCTCCAAACTCAGGGTTTACTGCACGATACTTACG
TTTACGGGGTAGATGCCAAGCGTATTTTACCCACCCTCATCTCCCCTACCGAAGTATTTGACGGCGCTATAGTTAGCGGT
AACTGTGTTTCGGCCTGTGACAAAAACACTACTTACGTGCACATGAATAACCCTGTAATTGAAGAATTATTTGCCCGTCA
CGGCACGGATATAAACTTTGTTGGCGTTATTATCACCAACGAAAACGTTACCTTAGCCGATAAAGAACGTTCCTCCAGCT
ACACCGCTAAACTTGCTGAAATGCTTGGAATTGACGCTGCAATCGTTTCCGAAGAAGGCTTCGGTAACCCTGATGCCGAC
CTGGTTATGAACTGCCGGAAGTTAGAGCAAAAAGGTATCAAAACCGTCCTTATCACCGACGAATACGCCGGTAGAGACGG
CAGCTCGCAATCGCTGGCCGACACCACTCCAGAAGCCAATGCCGTGGTTACCGCCGGTAACGCCAACGAAACCATTGTGC
TGCCCAAGTTAGATACTGTCATTGGCCATGTGGGAGTTGTCGATGTTATTGCCGGAGGTTTTGCCGGGTCACTACGGCCC
GATGGCAGCATCGAAGTGGAAATTCAAGCAATAACCGGCGCTACCTCGGAAGTTGGCTTCAATAAACTATCCGCAAAAGG
ATATTAA

Upstream 100 bases:

>100_bases
TTTATAAAAACGGCGAACTTGTAGACCAGCTACAAGGTAATTTTGGCCGCGAAGAAATTGAAGAAAAAATTCGGGCCATT
ATCGGCTAAGGAGGGATAAA

Downstream 100 bases:

>100_bases
CGACAGCCCACTAAACACCGTGATTGCTGTCCCTACTATAAAAACGAAAGGAGGAGTTTAGTATGGCAATTTTGGCCGGT
AAAAAAGTTATTGCTGTAGG

Product: glycine reductase proprotein GrdE

Products: NA

Alternate protein names: Selenoprotein PB alpha/beta [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MRLTLGKINIKGLEFGPSTRVENGVLYVNKDELTALLKEDNRISSVEVFLARPGESVRIVPVKDVIEPRVKVEGPGNIFP
GFLGKVEMVGSGRTHVLKGAAVVTTGKIVGFQEGIIDMTGPGAEYTPFSKTYNVVVKIEPVDGLSQHEHESVVRLAGLKA
AAYLGEAGKNITPDEEVTYEVAPLLEAASKYPGLPKVAYVYMLQTQGLLHDTYVYGVDAKRILPTLISPTEVFDGAIVSG
NCVSACDKNTTYVHMNNPVIEELFARHGTDINFVGVIITNENVTLADKERSSSYTAKLAEMLGIDAAIVSEEGFGNPDAD
LVMNCRKLEQKGIKTVLITDEYAGRDGSSQSLADTTPEANAVVTAGNANETIVLPKLDTVIGHVGVVDVIAGGFAGSLRP
DGSIEVEIQAITGATSEVGFNKLSAKGY

Sequences:

>Translated_428_residues
MRLTLGKINIKGLEFGPSTRVENGVLYVNKDELTALLKEDNRISSVEVFLARPGESVRIVPVKDVIEPRVKVEGPGNIFP
GFLGKVEMVGSGRTHVLKGAAVVTTGKIVGFQEGIIDMTGPGAEYTPFSKTYNVVVKIEPVDGLSQHEHESVVRLAGLKA
AAYLGEAGKNITPDEEVTYEVAPLLEAASKYPGLPKVAYVYMLQTQGLLHDTYVYGVDAKRILPTLISPTEVFDGAIVSG
NCVSACDKNTTYVHMNNPVIEELFARHGTDINFVGVIITNENVTLADKERSSSYTAKLAEMLGIDAAIVSEEGFGNPDAD
LVMNCRKLEQKGIKTVLITDEYAGRDGSSQSLADTTPEANAVVTAGNANETIVLPKLDTVIGHVGVVDVIAGGFAGSLRP
DGSIEVEIQAITGATSEVGFNKLSAKGY
>Mature_428_residues
MRLTLGKINIKGLEFGPSTRVENGVLYVNKDELTALLKEDNRISSVEVFLARPGESVRIVPVKDVIEPRVKVEGPGNIFP
GFLGKVEMVGSGRTHVLKGAAVVTTGKIVGFQEGIIDMTGPGAEYTPFSKTYNVVVKIEPVDGLSQHEHESVVRLAGLKA
AAYLGEAGKNITPDEEVTYEVAPLLEAASKYPGLPKVAYVYMLQTQGLLHDTYVYGVDAKRILPTLISPTEVFDGAIVSG
NCVSACDKNTTYVHMNNPVIEELFARHGTDINFVGVIITNENVTLADKERSSSYTAKLAEMLGIDAAIVSEEGFGNPDAD
LVMNCRKLEQKGIKTVLITDEYAGRDGSSQSLADTTPEANAVVTAGNANETIVLPKLDTVIGHVGVVDVIAGGFAGSLRP
DGSIEVEIQAITGATSEVGFNKLSAKGY

Specific function: In the first step of glycine reductase, the substrate is bound to component PB via a Schiff base intermediate. Then the PB- activated substrate is nucleophilically attacked by the selenol anion of component PA to transform it to a carboxymethylated seleno

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016585
- InterPro:   IPR015417 [H]

Pfam domain/function: PF09338 Gly_reductase [H]

EC number: =1.21.4.2 [H]

Molecular weight: Translated: 45582; Mature: 45582

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLTLGKINIKGLEFGPSTRVENGVLYVNKDELTALLKEDNRISSVEVFLARPGESVRIV
CEEEEEEEEEEEEECCCCCCCCCCEEEEEHHHHHHHHHCCCCCCEEEEEEECCCCCEEEE
PVKDVIEPRVKVEGPGNIFPGFLGKVEMVGSGRTHVLKGAAVVTTGKIVGFQEGIIDMTG
EHHHHHCCCEEECCCCCCCCCCCCEEEEECCCCEEEEECEEEEEECEEEEECCCCEEECC
PGAEYTPFSKTYNVVVKIEPVDGLSQHEHESVVRLAGLKAAAYLGEAGKNITPDEEVTYE
CCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHEEEH
VAPLLEAASKYPGLPKVAYVYMLQTQGLLHDTYVYGVDAKRILPTLISPTEVFDGAIVSG
HHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEEEECCHHHHHHHHCCCHHHHCCEEECC
NCVSACDKNTTYVHMNNPVIEELFARHGTDINFVGVIITNENVTLADKERSSSYTAKLAE
CCHHHCCCCCEEEEECCHHHHHHHHHCCCCEEEEEEEEECCCEEEECCCCCCHHHHHHHH
MLGIDAAIVSEEGFGNPDADLVMNCRKLEQKGIKTVLITDEYAGRDGSSQSLADTTPEAN
HHCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
AVVTAGNANETIVLPKLDTVIGHVGVVDVIAGGFAGSLRPDGSIEVEIQAITGATSEVGF
EEEEECCCCCEEEEECHHHHHHCCCHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCC
NKLSAKGY
CCCCCCCC
>Mature Secondary Structure
MRLTLGKINIKGLEFGPSTRVENGVLYVNKDELTALLKEDNRISSVEVFLARPGESVRIV
CEEEEEEEEEEEEECCCCCCCCCCEEEEEHHHHHHHHHCCCCCCEEEEEEECCCCCEEEE
PVKDVIEPRVKVEGPGNIFPGFLGKVEMVGSGRTHVLKGAAVVTTGKIVGFQEGIIDMTG
EHHHHHCCCEEECCCCCCCCCCCCEEEEECCCCEEEEECEEEEEECEEEEECCCCEEECC
PGAEYTPFSKTYNVVVKIEPVDGLSQHEHESVVRLAGLKAAAYLGEAGKNITPDEEVTYE
CCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHEEEH
VAPLLEAASKYPGLPKVAYVYMLQTQGLLHDTYVYGVDAKRILPTLISPTEVFDGAIVSG
HHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEEEECCHHHHHHHHCCCHHHHCCEEECC
NCVSACDKNTTYVHMNNPVIEELFARHGTDINFVGVIITNENVTLADKERSSSYTAKLAE
CCHHHCCCCCEEEEECCHHHHHHHHHCCCCEEEEEEEEECCCEEEECCCCCCHHHHHHHH
MLGIDAAIVSEEGFGNPDADLVMNCRKLEQKGIKTVLITDEYAGRDGSSQSLADTTPEAN
HHCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
AVVTAGNANETIVLPKLDTVIGHVGVVDVIAGGFAGSLRPDGSIEVEIQAITGATSEVGF
EEEEECCCCCEEEEECHHHHHHCCCHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCC
NKLSAKGY
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10091582; 8529639 [H]