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The map label for this gene is glnH [H]

Identifier: 78043559

GI number: 78043559

Start: 437331

End: 438149

Strand: Direct

Name: glnH [H]

Synonym: CHY_0504

Alternate gene names: 78043559

Gene position: 437331-438149 (Clockwise)

Preceding gene: 78044334

Following gene: 78044176

Centisome position: 18.21

GC content: 41.15

Gene sequence:

>819_bases
ATGAAAAAATTTGGAGCTTTGTTCTTGGTTGTCGTTTTAACCTTGGGGCTTATCCTGACAGCCTGTGGCAATAAGCAGGC
GGAGCAAACAAACAGCGCCCAAAACCAGGAGCAGAAGCAGGAGCAAAAACAAAAAATTGTAGTGGCTTCCGATACTTCTT
ACGCTCCTTTTGAATTTCAGGATCCTTCTACCGGAAAATATGTAGGTTTTGATATGGACTTAATTCAGGCAATTGGCGAA
GCGGTTGGCTTTGAAGTGGAAATTAAAAGTATGAACTTTGATGGAGTTTTAACTTCGGTGCAAAGTGGTACGGTTGATGC
GGCTATTTCCGCCATTTCGATTACCGATAAGCGCAAAGAAGTCATGAATTTTAGCGAACCGTATTACGATTCGGGTTTGG
CCATTGTGGTAAAAGGTGACAACAACACCATTAAAGGTATTGATGATTTAAAAGGGAAAAGGGTAGCGGTGCAAATTGCC
ACTACCGGTGCCGATTATGCCAACGAGTTAAAAGCTAAAGGAATTGTGAAAGAGGTAAAAACCTTTAATACTCTTCCCGA
TGCTTTAATGGAACTTAGAAACAACGGTGTTGATGCGGTAATTAACGACTGGCCGGTTAATGCCTACTATATTAAGCAAA
GCTTTTCCGATTTAAAAATGGTGGGGGATAAGTTAACCGGAGAATCGTATGGTATCGCTGTGCCCAAGTCCAAGCCGGAA
ATTTTAGCCAAAATTAACGAAGGCTTAAAGAAAGTGAAAGAATCGGGTAAGTACGCCGAAATTTACGAAAAGTGGTTTGG
AGAAAAACCAAAGAACTAA

Upstream 100 bases:

>100_bases
TCAGAAAATTTTAACATTGACTAATTGTACTAAACAATTTATAATTATTCAAAGGTTGATTCTTTAATATACTAAAGGGT
ATTAAAGGAGGAATTTTTTA

Downstream 100 bases:

>100_bases
TTGAGGTGGCTTAAGTGGAACTAAAATGGTCGGTAGTAATTGAATCTTTACCTTTATTAGCAGAAGGTGCTTTAGTAACG
CTTAAAATAACGGTTATTGC

Product: glutamine ABC transporter glutamine-binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: GlnBP [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKKFGALFLVVVLTLGLILTACGNKQAEQTNSAQNQEQKQEQKQKIVVASDTSYAPFEFQDPSTGKYVGFDMDLIQAIGE
AVGFEVEIKSMNFDGVLTSVQSGTVDAAISAISITDKRKEVMNFSEPYYDSGLAIVVKGDNNTIKGIDDLKGKRVAVQIA
TTGADYANELKAKGIVKEVKTFNTLPDALMELRNNGVDAVINDWPVNAYYIKQSFSDLKMVGDKLTGESYGIAVPKSKPE
ILAKINEGLKKVKESGKYAEIYEKWFGEKPKN

Sequences:

>Translated_272_residues
MKKFGALFLVVVLTLGLILTACGNKQAEQTNSAQNQEQKQEQKQKIVVASDTSYAPFEFQDPSTGKYVGFDMDLIQAIGE
AVGFEVEIKSMNFDGVLTSVQSGTVDAAISAISITDKRKEVMNFSEPYYDSGLAIVVKGDNNTIKGIDDLKGKRVAVQIA
TTGADYANELKAKGIVKEVKTFNTLPDALMELRNNGVDAVINDWPVNAYYIKQSFSDLKMVGDKLTGESYGIAVPKSKPE
ILAKINEGLKKVKESGKYAEIYEKWFGEKPKN
>Mature_272_residues
MKKFGALFLVVVLTLGLILTACGNKQAEQTNSAQNQEQKQEQKQKIVVASDTSYAPFEFQDPSTGKYVGFDMDLIQAIGE
AVGFEVEIKSMNFDGVLTSVQSGTVDAAISAISITDKRKEVMNFSEPYYDSGLAIVVKGDNNTIKGIDDLKGKRVAVQIA
TTGADYANELKAKGIVKEVKTFNTLPDALMELRNNGVDAVINDWPVNAYYIKQSFSDLKMVGDKLTGESYGIAVPKSKPE
ILAKINEGLKKVKESGKYAEIYEKWFGEKPKN

Specific function: Involved in a glutamine-transport system glnHPQ [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=229, Percent_Identity=44.9781659388646, Blast_Score=197, Evalue=9e-52,
Organism=Escherichia coli, GI1788228, Length=230, Percent_Identity=30.8695652173913, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787088, Length=230, Percent_Identity=33.0434782608696, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1787085, Length=232, Percent_Identity=30.6034482758621, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788649, Length=231, Percent_Identity=27.7056277056277, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788648, Length=231, Percent_Identity=28.5714285714286, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29802; Mature: 29802

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFGALFLVVVLTLGLILTACGNKQAEQTNSAQNQEQKQEQKQKIVVASDTSYAPFEFQ
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCEEEC
DPSTGKYVGFDMDLIQAIGEAVGFEVEIKSMNFDGVLTSVQSGTVDAAISAISITDKRKE
CCCCCCEECCCHHHHHHHHHHHCCEEEEEECCCCHHHHHHCCCCHHHHHHHEECCHHHHH
VMNFSEPYYDSGLAIVVKGDNNTIKGIDDLKGKRVAVQIATTGADYANELKAKGIVKEVK
HHCCCCCCCCCCEEEEEECCCCCCCCHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHH
TFNTLPDALMELRNNGVDAVINDWPVNAYYIKQSFSDLKMVGDKLTGESYGIAVPKSKPE
HHHHHHHHHHHHHCCCCCEEECCCCCCEEEEHHHHHHHHHHHHHCCCCCCEEECCCCCHH
ILAKINEGLKKVKESGKYAEIYEKWFGEKPKN
HHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKFGALFLVVVLTLGLILTACGNKQAEQTNSAQNQEQKQEQKQKIVVASDTSYAPFEFQ
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCEEEC
DPSTGKYVGFDMDLIQAIGEAVGFEVEIKSMNFDGVLTSVQSGTVDAAISAISITDKRKE
CCCCCCEECCCHHHHHHHHHHHCCEEEEEECCCCHHHHHHCCCCHHHHHHHEECCHHHHH
VMNFSEPYYDSGLAIVVKGDNNTIKGIDDLKGKRVAVQIATTGADYANELKAKGIVKEVK
HHCCCCCCCCCCEEEEEECCCCCCCCHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHH
TFNTLPDALMELRNNGVDAVINDWPVNAYYIKQSFSDLKMVGDKLTGESYGIAVPKSKPE
HHHHHHHHHHHHHCCCCCEEECCCCCCEEEEHHHHHHHHHHHHHCCCCCCEEECCCCCHH
ILAKINEGLKKVKESGKYAEIYEKWFGEKPKN
HHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]