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The map label for this gene is puuR [H]

Identifier: 78043349

GI number: 78043349

Start: 592764

End: 593588

Strand: Direct

Name: puuR [H]

Synonym: CHY_0677

Alternate gene names: 78043349

Gene position: 592764-593588 (Clockwise)

Preceding gene: 78044854

Following gene: 78043954

Centisome position: 24.68

GC content: 46.06

Gene sequence:

>825_bases
ATGTCTCCGGGTAAAACCTTTTTTACAATTGGTGATGCGGCAAAGCTAATTGGGGTGGTGCCAGCAACCCTTAGAAACTG
GGAAAAGGCGGGATTGGTAAAACCGGGCCGACAAAATGGGAACTACCGTATTTATTCCCTTAAAGATATTGAAAGGCTCA
AAAAAATTAAGTACTTAATGCGGGTTAAGGGTTTTAATATCGTTGGAGTGAAGGAATACCTGGCCGCGCAGGGGGAAGAA
TTGCCGGCAGAACCGGTTAAGATTGAGAAAAAGGGACCGAGGATAGCGGTCGGGCAAAAATTAAAGGCGCTTAGAGAAAA
AAAGGGTCTTACCCTGGAGGAAGTTTCCCAGGGAACTGGAGTTTCGGTTTCGTATTTAAGCCGAATTGAATCGGGGCAAG
TAAACGTTTCTATCGCTACCCTCCAGAAACTCGCCACCTTTTACGAAAAAAATCTCCTGTATTTTTTTGAGGGAATGGAT
ACCAAAGAGCAAAAATTGGTCCGGGCGGGCCTAGGTCAGGTGCTGGAAACTGACGAAGAGGGGATTAAGGTTGAGCTTTT
GACTGCTTTAAATGAACCGGCTATGGAGCCGCTCCTTTACACCGTAGCTCCGGGGGCGGGAAGAAATGAAAATATTTCCC
ATGAAGGGGAAGAGTTTGTCTACGTTTTAAAAGGGGTTTTAGAAGTGTGGCTTGATGAAACCGAACGTTACGTGGTTGGG
CCGGGGGATAGCCTTTATTTTAAAAGTACCCAGCAACACCGTTGGCGTAATATTGGCGATGAAGAACTGGTAGCCCTCTG
GATTAATACTCCGCCTACCTTCTAA

Upstream 100 bases:

>100_bases
TCGAAAAAAGACGAGAATCCTGAAAAGGCAATTTGTGAATATTTATACAAAAACATTTAAAAAGGTTTTTATATGTTGCG
GAAAGGAAGGAGAATTCCTG

Downstream 100 bases:

>100_bases
AAGGGTTATAAGCCCGAAAGGGCTCTAAATACCTAAAAAACTTAAAGGGGGTTTTAAAATGGGTTTTGATTTGGTTTTAA
AAAATGCCAAAATCCCTCAA

Product: MerR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSPGKTFFTIGDAAKLIGVVPATLRNWEKAGLVKPGRQNGNYRIYSLKDIERLKKIKYLMRVKGFNIVGVKEYLAAQGEE
LPAEPVKIEKKGPRIAVGQKLKALREKKGLTLEEVSQGTGVSVSYLSRIESGQVNVSIATLQKLATFYEKNLLYFFEGMD
TKEQKLVRAGLGQVLETDEEGIKVELLTALNEPAMEPLLYTVAPGAGRNENISHEGEEFVYVLKGVLEVWLDETERYVVG
PGDSLYFKSTQQHRWRNIGDEELVALWINTPPTF

Sequences:

>Translated_274_residues
MSPGKTFFTIGDAAKLIGVVPATLRNWEKAGLVKPGRQNGNYRIYSLKDIERLKKIKYLMRVKGFNIVGVKEYLAAQGEE
LPAEPVKIEKKGPRIAVGQKLKALREKKGLTLEEVSQGTGVSVSYLSRIESGQVNVSIATLQKLATFYEKNLLYFFEGMD
TKEQKLVRAGLGQVLETDEEGIKVELLTALNEPAMEPLLYTVAPGAGRNENISHEGEEFVYVLKGVLEVWLDETERYVVG
PGDSLYFKSTQQHRWRNIGDEELVALWINTPPTF
>Mature_273_residues
SPGKTFFTIGDAAKLIGVVPATLRNWEKAGLVKPGRQNGNYRIYSLKDIERLKKIKYLMRVKGFNIVGVKEYLAAQGEEL
PAEPVKIEKKGPRIAVGQKLKALREKKGLTLEEVSQGTGVSVSYLSRIESGQVNVSIATLQKLATFYEKNLLYFFEGMDT
KEQKLVRAGLGQVLETDEEGIKVELLTALNEPAMEPLLYTVAPGAGRNENISHEGEEFVYVLKGVLEVWLDETERYVVGP
GDSLYFKSTQQHRWRNIGDEELVALWINTPPTF

Specific function: Represses puuD [H]

COG id: COG1396

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787557, Length=181, Percent_Identity=26.5193370165746, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 30678; Mature: 30547

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS50943 HTH_CROC1 ; PS50937 HTH_MERR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPGKTFFTIGDAAKLIGVVPATLRNWEKAGLVKPGRQNGNYRIYSLKDIERLKKIKYLM
CCCCCEEEEECCHHHHHHHHHHHHHCHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHH
RVKGFNIVGVKEYLAAQGEELPAEPVKIEKKGPRIAVGQKLKALREKKGLTLEEVSQGTG
HHCCCCEEEHHHHHHCCCCCCCCCCEEEECCCCEEEHHHHHHHHHHHCCCCHHHHHCCCC
VSVSYLSRIESGQVNVSIATLQKLATFYEKNLLYFFEGMDTKEQKLVRAGLGQVLETDEE
CCHHHHHHHCCCCEEEHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHCCCCC
GIKVELLTALNEPAMEPLLYTVAPGAGRNENISHEGEEFVYVLKGVLEVWLDETERYVVG
CCEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEC
PGDSLYFKSTQQHRWRNIGDEELVALWINTPPTF
CCCCEEECCCHHHHHHCCCCCEEEEEEECCCCCC
>Mature Secondary Structure 
SPGKTFFTIGDAAKLIGVVPATLRNWEKAGLVKPGRQNGNYRIYSLKDIERLKKIKYLM
CCCCEEEEECCHHHHHHHHHHHHHCHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHH
RVKGFNIVGVKEYLAAQGEELPAEPVKIEKKGPRIAVGQKLKALREKKGLTLEEVSQGTG
HHCCCCEEEHHHHHHCCCCCCCCCCEEEECCCCEEEHHHHHHHHHHHCCCCHHHHHCCCC
VSVSYLSRIESGQVNVSIATLQKLATFYEKNLLYFFEGMDTKEQKLVRAGLGQVLETDEE
CCHHHHHHHCCCCEEEHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHCCCCC
GIKVELLTALNEPAMEPLLYTVAPGAGRNENISHEGEEFVYVLKGVLEVWLDETERYVVG
CCEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEC
PGDSLYFKSTQQHRWRNIGDEELVALWINTPPTF
CCCCEEECCCHHHHHHCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553 [H]