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The map label for this gene is gacS [H]

Identifier: 78043110

GI number: 78043110

Start: 523579

End: 525174

Strand: Direct

Name: gacS [H]

Synonym: CHY_0605

Alternate gene names: 78043110

Gene position: 523579-525174 (Clockwise)

Preceding gene: 78043912

Following gene: 78045027

Centisome position: 21.8

GC content: 41.54

Gene sequence:

>1596_bases
ATGATGCATGAAATTAAATTAAGCCAAAAGCTTATGCTTGGCATGGGCGTTGTTATAGGCTTTTTTTCGCTTCTGGCTTT
TATCTGGAACGTTGCCGAAACCCGCTATAGTTACCAGAAAGAGCTTTTAGAAAAAGCCCGGGTTATTACTTCCGGTCTGG
TGGCAACCCGCCGGGTAATCGCCGAAAATCAAGATAAAATAAATTATGACAGCCAAGGGCATTTTGAATTTAAACATTTA
AATCCGGCGGCGGTTGGACGACAGGTAGCAAAGTATTTCAATCAAACTACCGGCTACCGGCTAAAGCAAACCCGCCTTAA
ATACCGTAATCCCGAAAATGCCCCGGATCCCGTGGAAAAAGAAATGCTCAAAGAGCTGGCCGCAAACCCGCAAAAACAGG
AACTTTATCGCAACATAGAAAGGGATGGGCACCGTTATTTTTACTATATGAAACCTCTATACATTGAAGAGCCGTGCCTC
GATTGTCACGGCGAACCGGTGGGAGTCAAAGATATTGCCGGCTATCCCAAAGAAGGGTATAAACTTGGGGATTTTGCCGG
TGCTATTTCGCTGGTGGTTCCTGCGGATAATTTGCAAGTCGAGTTTGAACGTAAATTAAAAGGAAATTTCTTATTGTTTC
TTGCCTTTTTAGGTACTATCGGTCTTTTTAGCTTTACAGTTATAAAAAATACCATTGAAGAGCCGCTAAAAGAAATTGTG
GCTATGACTGGAGAGGTGGCTACCGGTAACTTTAAGACGAAAGTTATAGAAACTAATAGCCGGGAGATTAAAGAACTTTC
CGAAAACTTCCACCAGATGGCCCAGAAGTTAAAAGAACTTTATCATAACCTCGAGGAAAAAGTAAGAGAGCGAACTCATG
AGCTTTCCTTAGCCAATGAGGAGTTAGCCAGGGCCAACGCTTTTAAATCGGAGATAATTGCCATGGTTAGCCATGAGCTC
AAAACTCCGCTTACCAGCATTTTAGCCTTTGGTGAAATTCTTTTAGCCGACAGTGAAAACCTGCTTCCCTGGCAAAAAGA
GTATTTAGAAGATATAATGGAAAGCGGCCAGGAACTTTTAAAGCAAATTGAAACACTCTTAACTATGGCTAAAATTGAAG
CCGGAAAAATTACCGTTACCTATTCTCCGGTAAATTTGCAGCAATTTTTGGAGCAATGTTTTAAATTTTACAAAAATAAA
GCCAGGTCACGCCGGGTAAGCCTGAAAATTAAAACGAATATTACCGGAATTTTAATAACCGATGAACACAAACTGAAATT
AATCCTGGGAAATATAATCGGTAACGCTATTAAGTTTACTCCGCCGGGGGGGAAGATTTCGGTAGGATTGCGGAAAATCT
TTGGCCGCCTGTATATCTATGTGAAAGATTCCGGACCGGGAATTGCACCGGAAGACTTACCTAACATCTTTAATAAGTTC
TGGCGTAAAGATCAAAAGGTTGACGGTACTGGCCTGGGGCTTACCTTGGCAAAAGACCTGTGTGAACTGCTCGGTTACGA
AATTAAAGTAAAGTCGAAACTCGGTCGTGGAAGCAGTTTTTATATTGTTATTAAGGAAGGGTTTAGCGATGTATAA

Upstream 100 bases:

>100_bases
AAGAAATCTAAATGTTCCGGGCACTGCCCGGATTTTTTTTCTACGAATATTGGAGAAAAATTGTTATAATTATCGTAGAA
TGTAAAAAGGGGCAGTAAAT

Downstream 100 bases:

>100_bases
AATTTTAGTGGTGGAAGATGACCAGAAAATCCAAAAAGCTCTTGCCCATCTTTTAAAGGCTGAAAATTACGATTTTGTGC
AGGCGTACGATGGAGAGGCG

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MMHEIKLSQKLMLGMGVVIGFFSLLAFIWNVAETRYSYQKELLEKARVITSGLVATRRVIAENQDKINYDSQGHFEFKHL
NPAAVGRQVAKYFNQTTGYRLKQTRLKYRNPENAPDPVEKEMLKELAANPQKQELYRNIERDGHRYFYYMKPLYIEEPCL
DCHGEPVGVKDIAGYPKEGYKLGDFAGAISLVVPADNLQVEFERKLKGNFLLFLAFLGTIGLFSFTVIKNTIEEPLKEIV
AMTGEVATGNFKTKVIETNSREIKELSENFHQMAQKLKELYHNLEEKVRERTHELSLANEELARANAFKSEIIAMVSHEL
KTPLTSILAFGEILLADSENLLPWQKEYLEDIMESGQELLKQIETLLTMAKIEAGKITVTYSPVNLQQFLEQCFKFYKNK
ARSRRVSLKIKTNITGILITDEHKLKLILGNIIGNAIKFTPPGGKISVGLRKIFGRLYIYVKDSGPGIAPEDLPNIFNKF
WRKDQKVDGTGLGLTLAKDLCELLGYEIKVKSKLGRGSSFYIVIKEGFSDV

Sequences:

>Translated_531_residues
MMHEIKLSQKLMLGMGVVIGFFSLLAFIWNVAETRYSYQKELLEKARVITSGLVATRRVIAENQDKINYDSQGHFEFKHL
NPAAVGRQVAKYFNQTTGYRLKQTRLKYRNPENAPDPVEKEMLKELAANPQKQELYRNIERDGHRYFYYMKPLYIEEPCL
DCHGEPVGVKDIAGYPKEGYKLGDFAGAISLVVPADNLQVEFERKLKGNFLLFLAFLGTIGLFSFTVIKNTIEEPLKEIV
AMTGEVATGNFKTKVIETNSREIKELSENFHQMAQKLKELYHNLEEKVRERTHELSLANEELARANAFKSEIIAMVSHEL
KTPLTSILAFGEILLADSENLLPWQKEYLEDIMESGQELLKQIETLLTMAKIEAGKITVTYSPVNLQQFLEQCFKFYKNK
ARSRRVSLKIKTNITGILITDEHKLKLILGNIIGNAIKFTPPGGKISVGLRKIFGRLYIYVKDSGPGIAPEDLPNIFNKF
WRKDQKVDGTGLGLTLAKDLCELLGYEIKVKSKLGRGSSFYIVIKEGFSDV
>Mature_531_residues
MMHEIKLSQKLMLGMGVVIGFFSLLAFIWNVAETRYSYQKELLEKARVITSGLVATRRVIAENQDKINYDSQGHFEFKHL
NPAAVGRQVAKYFNQTTGYRLKQTRLKYRNPENAPDPVEKEMLKELAANPQKQELYRNIERDGHRYFYYMKPLYIEEPCL
DCHGEPVGVKDIAGYPKEGYKLGDFAGAISLVVPADNLQVEFERKLKGNFLLFLAFLGTIGLFSFTVIKNTIEEPLKEIV
AMTGEVATGNFKTKVIETNSREIKELSENFHQMAQKLKELYHNLEEKVRERTHELSLANEELARANAFKSEIIAMVSHEL
KTPLTSILAFGEILLADSENLLPWQKEYLEDIMESGQELLKQIETLLTMAKIEAGKITVTYSPVNLQQFLEQCFKFYKNK
ARSRRVSLKIKTNITGILITDEHKLKLILGNIIGNAIKFTPPGGKISVGLRKIFGRLYIYVKDSGPGIAPEDLPNIFNKF
WRKDQKVDGTGLGLTLAKDLCELLGYEIKVKSKLGRGSSFYIVIKEGFSDV

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=366, Percent_Identity=26.2295081967213, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87081816, Length=328, Percent_Identity=29.5731707317073, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI48994928, Length=234, Percent_Identity=34.1880341880342, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1786783, Length=280, Percent_Identity=27.1428571428571, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788393, Length=274, Percent_Identity=25.5474452554745, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1786600, Length=237, Percent_Identity=29.1139240506329, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1788713, Length=247, Percent_Identity=26.7206477732794, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI145693157, Length=244, Percent_Identity=29.0983606557377, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1786912, Length=259, Percent_Identity=26.2548262548263, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788279, Length=279, Percent_Identity=22.5806451612903, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1790436, Length=218, Percent_Identity=30.7339449541284, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI87082128, Length=230, Percent_Identity=28.695652173913, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790346, Length=271, Percent_Identity=25.830258302583, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788549, Length=220, Percent_Identity=26.8181818181818, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1787894, Length=220, Percent_Identity=27.7272727272727, Blast_Score=66, Evalue=7e-12,
Organism=Escherichia coli, GI1789403, Length=222, Percent_Identity=24.3243243243243, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1790567, Length=98, Percent_Identity=42.8571428571429, Blast_Score=63, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=196, Percent_Identity=32.1428571428571, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 60442; Mature: 60442

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMHEIKLSQKLMLGMGVVIGFFSLLAFIWNVAETRYSYQKELLEKARVITSGLVATRRVI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AENQDKINYDSQGHFEFKHLNPAAVGRQVAKYFNQTTGYRLKQTRLKYRNPENAPDPVEK
HCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHH
EMLKELAANPQKQELYRNIERDGHRYFYYMKPLYIEEPCLDCHGEPVGVKDIAGYPKEGY
HHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCEEECCCCCCCCCCCCCHHHHCCCCCCCC
KLGDFAGAISLVVPADNLQVEFERKLKGNFLLFLAFLGTIGLFSFTVIKNTIEEPLKEIV
CCCHHHCEEEEEEECCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMTGEVATGNFKTKVIETNSREIKELSENFHQMAQKLKELYHNLEEKVRERTHELSLANE
HHCCCEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELARANAFKSEIIAMVSHELKTPLTSILAFGEILLADSENLLPWQKEYLEDIMESGQELL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHH
KQIETLLTMAKIEAGKITVTYSPVNLQQFLEQCFKFYKNKARSRRVSLKIKTNITGILIT
HHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECEEEEEEE
DEHKLKLILGNIIGNAIKFTPPGGKISVGLRKIFGRLYIYVKDSGPGIAPEDLPNIFNKF
CCCHHHHHHHHHHCCCEEECCCCCEEEHHHHHHHCEEEEEEECCCCCCCHHHHHHHHHHH
WRKDQKVDGTGLGLTLAKDLCELLGYEIKVKSKLGRGSSFYIVIKEGFSDV
HCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MMHEIKLSQKLMLGMGVVIGFFSLLAFIWNVAETRYSYQKELLEKARVITSGLVATRRVI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AENQDKINYDSQGHFEFKHLNPAAVGRQVAKYFNQTTGYRLKQTRLKYRNPENAPDPVEK
HCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHH
EMLKELAANPQKQELYRNIERDGHRYFYYMKPLYIEEPCLDCHGEPVGVKDIAGYPKEGY
HHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCEEECCCCCCCCCCCCCHHHHCCCCCCCC
KLGDFAGAISLVVPADNLQVEFERKLKGNFLLFLAFLGTIGLFSFTVIKNTIEEPLKEIV
CCCHHHCEEEEEEECCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMTGEVATGNFKTKVIETNSREIKELSENFHQMAQKLKELYHNLEEKVRERTHELSLANE
HHCCCEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELARANAFKSEIIAMVSHELKTPLTSILAFGEILLADSENLLPWQKEYLEDIMESGQELL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHH
KQIETLLTMAKIEAGKITVTYSPVNLQQFLEQCFKFYKNKARSRRVSLKIKTNITGILIT
HHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECEEEEEEE
DEHKLKLILGNIIGNAIKFTPPGGKISVGLRKIFGRLYIYVKDSGPGIAPEDLPNIFNKF
CCCHHHHHHHHHHCCCEEECCCCCEEEHHHHHHHCEEEEEEECCCCCCCHHHHHHHHHHH
WRKDQKVDGTGLGLTLAKDLCELLGYEIKVKSKLGRGSSFYIVIKEGFSDV
HCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]