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The map label for this gene is smpB

Identifier: 78043075

GI number: 78043075

Start: 257939

End: 258388

Strand: Direct

Name: smpB

Synonym: CHY_0290

Alternate gene names: 78043075

Gene position: 257939-258388 (Clockwise)

Preceding gene: 78044313

Following gene: 78043735

Centisome position: 10.74

GC content: 40.22

Gene sequence:

>450_bases
ATGGAAAAGATAATTGCGGAAAATCGTAAAGCTTATCACGATTATCATATTCTCGAAAAATACGAGGCAGGAATAGCATT
AAAAGGAACCGAGGTTAAATCCATTCGCCTTGGCCGGGTAAATTTAAGGGATAGTTTTGCCCGGATTGAAAACGGTGAGC
TCTGGCTTTACAACATGCACATAAGTCCCTATGAGCAGGGGAACCGCTTTAACCACGAACCTAAAAGACCCCGAAAGCTA
TTAATGCATAAAAGGGAGATTATGCGCCTTTTTGGTAAGACCAGGGAAAAGGGTCTAACTTTGGTACCATTAAAGCTTTA
CTTTAAAGGAAATTATGCTAAAATAGAATTGGGCTTAGCCAAAGGTAAAAAGATTTACGACAAGCGGGAAGAGATGGCAA
AAAGGGATGCAGCAAGAGAAATTGAAAAGGCTCTTCGAGCTCGTTATTAA

Upstream 100 bases:

>100_bases
GGAGCGGTTAACGTAAAAAAATTTCTGAGCAAAGGTTTTTGAAAATATGATATAATAATTTAAGTCATGTATGTTACGAG
ATTTTAAGGGGTATGTTTTT

Downstream 100 bases:

>100_bases
CCTGGGGGCGTAAAGGGTTCGACGGGGGAGCGAAGGGCCGAAAGTAGCGAGGCGAGGACCCCATCTTACCTCGTTAAAAA
TGGTGGGAAAAGATAACTGC

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MEKIIAENRKAYHDYHILEKYEAGIALKGTEVKSIRLGRVNLRDSFARIENGELWLYNMHISPYEQGNRFNHEPKRPRKL
LMHKREIMRLFGKTREKGLTLVPLKLYFKGNYAKIELGLAKGKKIYDKREEMAKRDAAREIEKALRARY

Sequences:

>Translated_149_residues
MEKIIAENRKAYHDYHILEKYEAGIALKGTEVKSIRLGRVNLRDSFARIENGELWLYNMHISPYEQGNRFNHEPKRPRKL
LMHKREIMRLFGKTREKGLTLVPLKLYFKGNYAKIELGLAKGKKIYDKREEMAKRDAAREIEKALRARY
>Mature_149_residues
MEKIIAENRKAYHDYHILEKYEAGIALKGTEVKSIRLGRVNLRDSFARIENGELWLYNMHISPYEQGNRFNHEPKRPRKL
LMHKREIMRLFGKTREKGLTLVPLKLYFKGNYAKIELGLAKGKKIYDKREEMAKRDAAREIEKALRARY

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=147, Percent_Identity=41.4965986394558, Blast_Score=118, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_CARHZ (Q3AFC2)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359162.1
- ProteinModelPortal:   Q3AFC2
- SMR:   Q3AFC2
- STRING:   Q3AFC2
- GeneID:   3726392
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0290
- NMPDR:   fig|246194.3.peg.339
- TIGR:   CHY_0290
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- BioCyc:   CHYD246194:CHY_0290-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 17720; Mature: 17720

Theoretical pI: Translated: 10.57; Mature: 10.57

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIIAENRKAYHDYHILEKYEAGIALKGTEVKSIRLGRVNLRDSFARIENGELWLYNMH
CCCCHHCCCHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCHHHHHHHCCCCCEEEEEEE
ISPYEQGNRFNHEPKRPRKLLMHKREIMRLFGKTREKGLTLVPLKLYFKGNYAKIELGLA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCEEEEEEECC
KGKKIYDKREEMAKRDAAREIEKALRARY
CCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKIIAENRKAYHDYHILEKYEAGIALKGTEVKSIRLGRVNLRDSFARIENGELWLYNMH
CCCCHHCCCHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCHHHHHHHCCCCCEEEEEEE
ISPYEQGNRFNHEPKRPRKLLMHKREIMRLFGKTREKGLTLVPLKLYFKGNYAKIELGLA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCEEEEEEECC
KGKKIYDKREEMAKRDAAREIEKALRARY
CCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA