Click here to switch to the map view.

The map label for this gene is ilvB2 [H]

Identifier: 78043014

GI number: 78043014

Start: 451183

End: 452847

Strand: Direct

Name: ilvB2 [H]

Synonym: CHY_0520

Alternate gene names: 78043014

Gene position: 451183-452847 (Clockwise)

Preceding gene: 78042750

Following gene: 78044251

Centisome position: 18.79

GC content: 47.15

Gene sequence:

>1665_bases
TTGGAGATAACTGGTGCTCAGGCTTTGGTAAAAGCGTTAGAACTGGAGGGGGTAACTACTATCTTCGGTTACCCTGGAGG
AGCGGTTGTACCCCTTTATGATGCCTTAAGCCGATCCAAAATTCAGCATGTTTTGGTGCGCCATGAGCCGGGAGCAATTC
ATGCTGCTAATGGTTATGCCCGGGCTTCGGGGAAAGTTGGGGTATGTGTTGCCACCTCGGGCCCGGGGGCAACCAACCTG
GTTACGGGGATTGCTACGGCATATATGGATTCGGTACCGGTGGTGGTGATTACCGGGCAAGTACCGACAACGATGGTGGG
CACCGATGCGTTTCAGGAAGTGGATATTACCGGAATTACCATGCCCATCACCAAACATAACTTTTTGGTAAAAAATCCTG
CAGATATTCCGGCGGTGGTGAAAAAGGCTTTTTATATCGCTGCCACGGGACGACCGGGGCCGGTACTGATTGATTTACCC
AAAGATGTAGCAGCGCGGACCATAGATTTTCAGTATCCTGAAAAAGTAGAAATTCGCGGTTACAAGCCTAATTTAAAAGG
GCATCCGCAAAAAATTGCCGAAGCGGTAAAGCTCATAAGGGAAGCGAAAAGGCCGGTAATTATTGCCGGAGGCGGTGTGC
TTGCGGCCAATGCCAGTCAGGAGCTTTATGAATTTGCGGTAAGAGCTGAGATTCCGGTAACTAATACGCTAATGGGACTT
ACTTCCTTTCCCCAGGATAGTCCGCTTTTCCTGGGCATGCTTGGCCTGCATGGAACCAGGTATGCCAATTTAGCGGTGAC
CGAATGTGATGTTTTAATTGGACTGGGAGTGCGTTTTGCCGACCGGGTAACGGGAGAGTTATCCGGCTTTGCTCCCAAAG
CAAAAATTATCCATATCGATGTAGATCCGGCGGAAATCGGCAAGAACGTGCGGGCGGATGTTCCCATTGTTGGTGATGTC
AAAAACGTTTTGCAGGAGATGTTAAAGCAAATTGAGCCGCAGAATCGTCAGGAATGGCTTTCCCAAATTAACACCTGGAA
GCAAGAGTTTCCCTTAAAGTATGAAAAAGATGATTTTATCAAACCCCAGGAGACTATTAAGCTTTTGGGGGAAAAGACCA
CCGCCGACACTATAATCGCTACCGATGTGGGACAGCATCAGATGTGGACAGCGCAATTTTATCCCTTTAAAAAACCCCGT
ACTTTCTTAACCAGCGGGGGTCTTGGCTGCATGGGTTATGGTTTGCCGGCAGCTATAGGTGCGGCGGTAGCTTTTCGGGA
TAAACAGGTAATTTTAATTACCGGCGATGGCAGTTTCCAAATGAATATGGGGGAGTTGGCTACTGCCCGGGAACAGAATT
TGTCGTTGAAGATTATTTTGTTTGTTAATAAAAAATTAGGTATGGTAAAGCAGTTGCAGGAATATTATGCCAATAAAAAT
TACTTTGGGGTTGACTTGGGCTTTGTTCCGGATTTTGTAGCTTTAGCTAAGGCTTATGGAATTCCCGGACGGAAGATTAC
CAGACCAGAAGAGGTTGACGGGGCTTTAGATGAAATGCTTGCCCATAAGGGAATGTATCTTTTGGAGATTGAAATCAGTC
CTGAAGAAAAAGTTTTACCCATGGTTTTATCCGGGGCTCCTATAGGAGAAGCGGTGGACTGGTAA

Upstream 100 bases:

>100_bases
TAACGCCATCAAGCGTCGGGAACAGGAACATTTAATTGAAAAGGTGGGGGCAGAATTACGGCAGATGATGCCATGGCTGA
AAAAGTAGGGGGGATGTCTT

Downstream 100 bases:

>100_bases
TCTTGAAGTTTGAAGTTGGAAATTAGAGGTTAGAATAAAACGAAAAAACATTTAAAGTGTGGTGAGGTAAATGGAAAGGG
TTAGGATTTTTGATACGACG

Product: acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MEITGAQALVKALELEGVTTIFGYPGGAVVPLYDALSRSKIQHVLVRHEPGAIHAANGYARASGKVGVCVATSGPGATNL
VTGIATAYMDSVPVVVITGQVPTTMVGTDAFQEVDITGITMPITKHNFLVKNPADIPAVVKKAFYIAATGRPGPVLIDLP
KDVAARTIDFQYPEKVEIRGYKPNLKGHPQKIAEAVKLIREAKRPVIIAGGGVLAANASQELYEFAVRAEIPVTNTLMGL
TSFPQDSPLFLGMLGLHGTRYANLAVTECDVLIGLGVRFADRVTGELSGFAPKAKIIHIDVDPAEIGKNVRADVPIVGDV
KNVLQEMLKQIEPQNRQEWLSQINTWKQEFPLKYEKDDFIKPQETIKLLGEKTTADTIIATDVGQHQMWTAQFYPFKKPR
TFLTSGGLGCMGYGLPAAIGAAVAFRDKQVILITGDGSFQMNMGELATAREQNLSLKIILFVNKKLGMVKQLQEYYANKN
YFGVDLGFVPDFVALAKAYGIPGRKITRPEEVDGALDEMLAHKGMYLLEIEISPEEKVLPMVLSGAPIGEAVDW

Sequences:

>Translated_554_residues
MEITGAQALVKALELEGVTTIFGYPGGAVVPLYDALSRSKIQHVLVRHEPGAIHAANGYARASGKVGVCVATSGPGATNL
VTGIATAYMDSVPVVVITGQVPTTMVGTDAFQEVDITGITMPITKHNFLVKNPADIPAVVKKAFYIAATGRPGPVLIDLP
KDVAARTIDFQYPEKVEIRGYKPNLKGHPQKIAEAVKLIREAKRPVIIAGGGVLAANASQELYEFAVRAEIPVTNTLMGL
TSFPQDSPLFLGMLGLHGTRYANLAVTECDVLIGLGVRFADRVTGELSGFAPKAKIIHIDVDPAEIGKNVRADVPIVGDV
KNVLQEMLKQIEPQNRQEWLSQINTWKQEFPLKYEKDDFIKPQETIKLLGEKTTADTIIATDVGQHQMWTAQFYPFKKPR
TFLTSGGLGCMGYGLPAAIGAAVAFRDKQVILITGDGSFQMNMGELATAREQNLSLKIILFVNKKLGMVKQLQEYYANKN
YFGVDLGFVPDFVALAKAYGIPGRKITRPEEVDGALDEMLAHKGMYLLEIEISPEEKVLPMVLSGAPIGEAVDW
>Mature_554_residues
MEITGAQALVKALELEGVTTIFGYPGGAVVPLYDALSRSKIQHVLVRHEPGAIHAANGYARASGKVGVCVATSGPGATNL
VTGIATAYMDSVPVVVITGQVPTTMVGTDAFQEVDITGITMPITKHNFLVKNPADIPAVVKKAFYIAATGRPGPVLIDLP
KDVAARTIDFQYPEKVEIRGYKPNLKGHPQKIAEAVKLIREAKRPVIIAGGGVLAANASQELYEFAVRAEIPVTNTLMGL
TSFPQDSPLFLGMLGLHGTRYANLAVTECDVLIGLGVRFADRVTGELSGFAPKAKIIHIDVDPAEIGKNVRADVPIVGDV
KNVLQEMLKQIEPQNRQEWLSQINTWKQEFPLKYEKDDFIKPQETIKLLGEKTTADTIIATDVGQHQMWTAQFYPFKKPR
TFLTSGGLGCMGYGLPAAIGAAVAFRDKQVILITGDGSFQMNMGELATAREQNLSLKIILFVNKKLGMVKQLQEYYANKN
YFGVDLGFVPDFVALAKAYGIPGRKITRPEEVDGALDEMLAHKGMYLLEIEISPEEKVLPMVLSGAPIGEAVDW

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=569, Percent_Identity=26.0105448154657, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI21361361, Length=490, Percent_Identity=24.4897959183673, Blast_Score=136, Evalue=4e-32,
Organism=Escherichia coli, GI87081685, Length=562, Percent_Identity=45.729537366548, Blast_Score=520, Evalue=1e-148,
Organism=Escherichia coli, GI1790104, Length=551, Percent_Identity=46.0980036297641, Blast_Score=477, Evalue=1e-135,
Organism=Escherichia coli, GI1786717, Length=547, Percent_Identity=35.6489945155393, Blast_Score=327, Evalue=9e-91,
Organism=Escherichia coli, GI1787096, Length=553, Percent_Identity=29.8372513562387, Blast_Score=221, Evalue=8e-59,
Organism=Escherichia coli, GI1788716, Length=487, Percent_Identity=27.7207392197125, Blast_Score=159, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17542570, Length=507, Percent_Identity=25.2465483234714, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17531299, Length=480, Percent_Identity=26.25, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17531301, Length=480, Percent_Identity=26.0416666666667, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6323755, Length=578, Percent_Identity=45.6747404844291, Blast_Score=469, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6320816, Length=497, Percent_Identity=26.5593561368209, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6323163, Length=539, Percent_Identity=24.6753246753247, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6321524, Length=549, Percent_Identity=24.7723132969035, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19922626, Length=492, Percent_Identity=26.8292682926829, Blast_Score=160, Evalue=3e-39,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60010; Mature: 60010

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEITGAQALVKALELEGVTTIFGYPGGAVVPLYDALSRSKIQHVLVRHEPGAIHAANGYA
CCCCHHHHHHHHHHHCCEEEEEECCCCCEEHHHHHHHHHHHHHEEEECCCCEEEECCCEE
RASGKVGVCVATSGPGATNLVTGIATAYMDSVPVVVITGQVPTTMVGTDAFQEVDITGIT
ECCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCHHEECEEEEE
MPITKHNFLVKNPADIPAVVKKAFYIAATGRPGPVLIDLPKDVAARTIDFQYPEKVEIRG
EEEECCCEEEECCCCCHHHHHCEEEEEECCCCCCEEEECCHHHHHEEECCCCCCEEEEEE
YKPNLKGHPQKIAEAVKLIREAKRPVIIAGGGVLAANASQELYEFAVRAEIPVTNTLMGL
ECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEECCHHHHHHHHHHCCCCCHHHHHHH
TSFPQDSPLFLGMLGLHGTRYANLAVTECDVLIGLGVRFADRVTGELSGFAPKAKIIHID
CCCCCCCCEEEEEECCCCCEEEEEEEEEEEEEEECCCHHHHHHHHHHCCCCCCCEEEEEE
VDPAEIGKNVRADVPIVGDVKNVLQEMLKQIEPQNRQEWLSQINTWKQEFPLKYEKDDFI
CCHHHHCCCCCCCCCEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
KPQETIKLLGEKTTADTIIATDVGQHQMWTAQFYPFKKPRTFLTSGGLGCMGYGLPAAIG
CHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCHHHHHH
AAVAFRDKQVILITGDGSFQMNMGELATAREQNLSLKIILFVNKKLGMVKQLQEYYANKN
HHEEECCCEEEEEECCCCEEECHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHCCCC
YFGVDLGFVPDFVALAKAYGIPGRKITRPEEVDGALDEMLAHKGMYLLEIEISPEEKVLP
EEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEECCCCCEEE
MVLSGAPIGEAVDW
EEECCCCCCCCCCC
>Mature Secondary Structure
MEITGAQALVKALELEGVTTIFGYPGGAVVPLYDALSRSKIQHVLVRHEPGAIHAANGYA
CCCCHHHHHHHHHHHCCEEEEEECCCCCEEHHHHHHHHHHHHHEEEECCCCEEEECCCEE
RASGKVGVCVATSGPGATNLVTGIATAYMDSVPVVVITGQVPTTMVGTDAFQEVDITGIT
ECCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCHHEECEEEEE
MPITKHNFLVKNPADIPAVVKKAFYIAATGRPGPVLIDLPKDVAARTIDFQYPEKVEIRG
EEEECCCEEEECCCCCHHHHHCEEEEEECCCCCCEEEECCHHHHHEEECCCCCCEEEEEE
YKPNLKGHPQKIAEAVKLIREAKRPVIIAGGGVLAANASQELYEFAVRAEIPVTNTLMGL
ECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEECCHHHHHHHHHHCCCCCHHHHHHH
TSFPQDSPLFLGMLGLHGTRYANLAVTECDVLIGLGVRFADRVTGELSGFAPKAKIIHID
CCCCCCCCEEEEEECCCCCEEEEEEEEEEEEEEECCCHHHHHHHHHHCCCCCCCEEEEEE
VDPAEIGKNVRADVPIVGDVKNVLQEMLKQIEPQNRQEWLSQINTWKQEFPLKYEKDDFI
CCHHHHCCCCCCCCCEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
KPQETIKLLGEKTTADTIIATDVGQHQMWTAQFYPFKKPRTFLTSGGLGCMGYGLPAAIG
CHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCHHHHHH
AAVAFRDKQVILITGDGSFQMNMGELATAREQNLSLKIILFVNKKLGMVKQLQEYYANKN
HHEEECCCEEEEEECCCCEEECHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHCCCC
YFGVDLGFVPDFVALAKAYGIPGRKITRPEEVDGALDEMLAHKGMYLLEIEISPEEKVLP
EEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEECCCCCEEE
MVLSGAPIGEAVDW
EEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]