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The map label for this gene is leuA

Identifier: 78044251

GI number: 78044251

Start: 452918

End: 454447

Strand: Direct

Name: leuA

Synonym: CHY_0521

Alternate gene names: 78044251

Gene position: 452918-454447 (Clockwise)

Preceding gene: 78043014

Following gene: 78043190

Centisome position: 18.86

GC content: 45.36

Gene sequence:

>1530_bases
ATGGAAAGGGTTAGGATTTTTGATACGACGTTAAGGGATGGGGAACAGTCGCCAGGAGTTAGTTTAAATGCTATGGAGAA
ATTGCAAATCGCCCGGCAGTTACAAAAGCTGGGCGTTGATGTTATAGAGGCAGGTTTTCCTATAACTTCTCCCGGAGATA
AGGAAGCCGTTTCCTTAATTGCCCGGGAAGTAAAAGGGGTGGTAGTGGCTGCCCTGGCCCGGGCTTCGGCTTTGGATATA
GAAACGGCCTGGGACGCCATTAAAGATGCGGAAAGTCCCCGGATTCATACTTTTATTGCCACTTCCGATATTCATTTGAA
ATATAAGCTGAAAATGGACCGGGAAACGGTAGTGGAACGGGCGGTAGCAGCGGTCAAACTTGCTAAAAAATTTACCGCCG
ATGTGGAGTTTTCGGCGGAAGATGCTTCAAGAAGTGACCTGGACTTTTTGTGCCGGGTGGTGGAAGCGGCGGTAAAAGCG
GGAGCTACTACCATAAACATTCCCGATACCGTTGGCTATGCCGAACCGGAAGAGTTTGGGGAGTTTATTTGCAAAATTTT
AGAAAAAGTTCCGGTAATGGACCGGGCGGTGTTAAGCGTTCACTGTCATGATGATTTGGGCCTTGCGGTGGCCAATAGTT
TAGCTGCCATCAAAAACGGCGCCCGGCAGGTGGAATGTACCATTAACGGAATTGGTGAACGGGCTGGAAACTGTTCCTTA
GAGGAGATTGTAATGGCTCTTTATACCCGCAAAGATGTATTGCCTTTTTATACCGGCATTAAAACCGAGGAGATTTACCG
GACCAGTAAGCTCGTTAGCAATTTAACCGGGATGCCGGTACAGCCCAACAAAGCGATTGTAGGGAAAAATGCCTTTTCCC
ACGAATCGGGGATTCACCAGGATGGGGTTTTAAAGGAAAGAACTACTTATGAAATAATGAATCCCAGGCTGGTGGGTATT
CCCGAATCCCGTCTGGTACTGGGTAAACATTCGGGAAGACATGCGTTAAAGGAGAGGTTATTAGAGCTTGGTTATGAATT
AACCGAAGAGCAGTTGAATGAGGCTTTTGTTAAATTTAAAGCTTTAGCCGATAAGAAAAAAGAAGTTACCGATCAGGATT
TAGAAGCAATGATGGAAGAAGAAATAAGAAAAGTTCCCGAAACTTACACCCTGGATTATTTCCATATTTCTACCGGTTCA
ACGATTATTCCCACGGCCACGGTGGGCTTAATCAAGGAAGGGGAAAAATTAGAAGACGCCGCCACCGGCGATGGACCGGT
GGATGCCATTTATAAAGCTATCAATAAAATAACCGGTTTAACTCCGGTTTTAGAGCAATATTCAATTAATGCCGTAACTT
CTGGCGAAGATGCCCTGGGAGAAGTAGTGGTAAAACTAAAAAATGGCCTCGGGAAAATTGTCACCGGTCGCGGAGTTTCC
ACCGATATTTTAGAAGCATCGGCCAAGGCTTATTTAAATGGTATCAATAAATTACTTTTTGATTACCAGGCGAAAGGGGA
GAAGCAGTAA

Upstream 100 bases:

>100_bases
GCTCCTATAGGAGAAGCGGTGGACTGGTAATCTTGAAGTTTGAAGTTGGAAATTAGAGGTTAGAATAAAACGAAAAAACA
TTTAAAGTGTGGTGAGGTAA

Downstream 100 bases:

>100_bases
TGGGGATGACGATGACCGAGAAAATTCTTGCCTATCATGCCGGAAAAGAGCTTGTTGAACCGGGAGAATTAATTTCCTGC
AAGGTAGATGCGGTTTTAGC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MERVRIFDTTLRDGEQSPGVSLNAMEKLQIARQLQKLGVDVIEAGFPITSPGDKEAVSLIAREVKGVVVAALARASALDI
ETAWDAIKDAESPRIHTFIATSDIHLKYKLKMDRETVVERAVAAVKLAKKFTADVEFSAEDASRSDLDFLCRVVEAAVKA
GATTINIPDTVGYAEPEEFGEFICKILEKVPVMDRAVLSVHCHDDLGLAVANSLAAIKNGARQVECTINGIGERAGNCSL
EEIVMALYTRKDVLPFYTGIKTEEIYRTSKLVSNLTGMPVQPNKAIVGKNAFSHESGIHQDGVLKERTTYEIMNPRLVGI
PESRLVLGKHSGRHALKERLLELGYELTEEQLNEAFVKFKALADKKKEVTDQDLEAMMEEEIRKVPETYTLDYFHISTGS
TIIPTATVGLIKEGEKLEDAATGDGPVDAIYKAINKITGLTPVLEQYSINAVTSGEDALGEVVVKLKNGLGKIVTGRGVS
TDILEASAKAYLNGINKLLFDYQAKGEKQ

Sequences:

>Translated_509_residues
MERVRIFDTTLRDGEQSPGVSLNAMEKLQIARQLQKLGVDVIEAGFPITSPGDKEAVSLIAREVKGVVVAALARASALDI
ETAWDAIKDAESPRIHTFIATSDIHLKYKLKMDRETVVERAVAAVKLAKKFTADVEFSAEDASRSDLDFLCRVVEAAVKA
GATTINIPDTVGYAEPEEFGEFICKILEKVPVMDRAVLSVHCHDDLGLAVANSLAAIKNGARQVECTINGIGERAGNCSL
EEIVMALYTRKDVLPFYTGIKTEEIYRTSKLVSNLTGMPVQPNKAIVGKNAFSHESGIHQDGVLKERTTYEIMNPRLVGI
PESRLVLGKHSGRHALKERLLELGYELTEEQLNEAFVKFKALADKKKEVTDQDLEAMMEEEIRKVPETYTLDYFHISTGS
TIIPTATVGLIKEGEKLEDAATGDGPVDAIYKAINKITGLTPVLEQYSINAVTSGEDALGEVVVKLKNGLGKIVTGRGVS
TDILEASAKAYLNGINKLLFDYQAKGEKQ
>Mature_509_residues
MERVRIFDTTLRDGEQSPGVSLNAMEKLQIARQLQKLGVDVIEAGFPITSPGDKEAVSLIAREVKGVVVAALARASALDI
ETAWDAIKDAESPRIHTFIATSDIHLKYKLKMDRETVVERAVAAVKLAKKFTADVEFSAEDASRSDLDFLCRVVEAAVKA
GATTINIPDTVGYAEPEEFGEFICKILEKVPVMDRAVLSVHCHDDLGLAVANSLAAIKNGARQVECTINGIGERAGNCSL
EEIVMALYTRKDVLPFYTGIKTEEIYRTSKLVSNLTGMPVQPNKAIVGKNAFSHESGIHQDGVLKERTTYEIMNPRLVGI
PESRLVLGKHSGRHALKERLLELGYELTEEQLNEAFVKFKALADKKKEVTDQDLEAMMEEEIRKVPETYTLDYFHISTGS
TIIPTATVGLIKEGEKLEDAATGDGPVDAIYKAINKITGLTPVLEQYSINAVTSGEDALGEVVVKLKNGLGKIVTGRGVS
TDILEASAKAYLNGINKLLFDYQAKGEKQ

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=503, Percent_Identity=53.2803180914513, Blast_Score=511, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6320071, Length=382, Percent_Identity=28.7958115183246, Blast_Score=156, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6320019, Length=366, Percent_Identity=29.5081967213115, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6324682, Length=546, Percent_Identity=25.2747252747253, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324225, Length=539, Percent_Identity=25.0463821892393, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24582381, Length=292, Percent_Identity=25.3424657534247, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_CARHZ (Q3AEQ5)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359379.1
- ProteinModelPortal:   Q3AEQ5
- SMR:   Q3AEQ5
- STRING:   Q3AEQ5
- GeneID:   3727368
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0521
- NMPDR:   fig|246194.3.peg.1088
- TIGR:   CHY_0521
- eggNOG:   COG0119
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- ProtClustDB:   PRK00915
- BioCyc:   CHYD246194:CHY_0521-MONOMER
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 55563; Mature: 55563

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERVRIFDTTLRDGEQSPGVSLNAMEKLQIARQLQKLGVDVIEAGFPITSPGDKEAVSLI
CCCEEEEECHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHH
AREVKGVVVAALARASALDIETAWDAIKDAESPRIHTFIATSDIHLKYKLKMDRETVVER
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEECCEEEEEEEECCHHHHHHH
AVAAVKLAKKFTADVEFSAEDASRSDLDFLCRVVEAAVKAGATTINIPDTVGYAEPEEFG
HHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHH
EFICKILEKVPVMDRAVLSVHCHDDLGLAVANSLAAIKNGARQVECTINGIGERAGNCSL
HHHHHHHHHCCCCCHHEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCH
EEIVMALYTRKDVLPFYTGIKTEEIYRTSKLVSNLTGMPVQPNKAIVGKNAFSHESGIHQ
HHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCC
DGVLKERTTYEIMNPRLVGIPESRLVLGKHSGRHALKERLLELGYELTEEQLNEAFVKFK
CCCCCCCCHHHCCCCEEECCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALADKKKEVTDQDLEAMMEEEIRKVPETYTLDYFHISTGSTIIPTATVGLIKEGEKLEDA
HHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCEECCHHHHHHHHCCCHHHHC
ATGDGPVDAIYKAINKITGLTPVLEQYSINAVTSGEDALGEVVVKLKNGLGKIVTGRGVS
CCCCCHHHHHHHHHHHHCCCHHHHHHHCCCEECCCHHHHHHHHHHHHHCCCCEEECCCCC
TDILEASAKAYLNGINKLLFDYQAKGEKQ
HHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MERVRIFDTTLRDGEQSPGVSLNAMEKLQIARQLQKLGVDVIEAGFPITSPGDKEAVSLI
CCCEEEEECHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHH
AREVKGVVVAALARASALDIETAWDAIKDAESPRIHTFIATSDIHLKYKLKMDRETVVER
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEECCEEEEEEEECCHHHHHHH
AVAAVKLAKKFTADVEFSAEDASRSDLDFLCRVVEAAVKAGATTINIPDTVGYAEPEEFG
HHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHH
EFICKILEKVPVMDRAVLSVHCHDDLGLAVANSLAAIKNGARQVECTINGIGERAGNCSL
HHHHHHHHHCCCCCHHEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCH
EEIVMALYTRKDVLPFYTGIKTEEIYRTSKLVSNLTGMPVQPNKAIVGKNAFSHESGIHQ
HHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCC
DGVLKERTTYEIMNPRLVGIPESRLVLGKHSGRHALKERLLELGYELTEEQLNEAFVKFK
CCCCCCCCHHHCCCCEEECCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALADKKKEVTDQDLEAMMEEEIRKVPETYTLDYFHISTGSTIIPTATVGLIKEGEKLEDA
HHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCEECCHHHHHHHHCCCHHHHC
ATGDGPVDAIYKAINKITGLTPVLEQYSINAVTSGEDALGEVVVKLKNGLGKIVTGRGVS
CCCCCHHHHHHHHHHHHCCCHHHHHHHCCCEECCCHHHHHHHHHHHHHCCCCEEECCCCC
TDILEASAKAYLNGINKLLFDYQAKGEKQ
HHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA