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The map label for this gene is glnA1 [H]

Identifier: 78042986

GI number: 78042986

Start: 618416

End: 619744

Strand: Direct

Name: glnA1 [H]

Synonym: CHY_0704

Alternate gene names: 78042986

Gene position: 618416-619744 (Clockwise)

Preceding gene: 78043628

Following gene: 78043307

Centisome position: 25.75

GC content: 46.2

Gene sequence:

>1329_bases
ATGAGTTACACCAAAAACGATATTATGGATATGGTGCGGGAGCAAAATGTTAAGTTTATCCGGCTCCAGTTTACCGACAT
TTTTGGGGTTCTAAAAAATGTGGCCATTCCGGTAGAACAGTTAGAAAAGGCCTTAAACAACGAGCTAATGTTTGACGGCT
CCAGTATTGAAGGTTTTGTCCGTATTGAAGAGTCGGATATGTATTTGCGTCCGGACCCCAATACTTTTGCCGTATTTCCC
TGGCGGCCCAGAAACGGTGCGGTAGCCCGGCTGATTTGCGATGTGTACAATCCCGATGGTACTCCCTTTGAGGGCGACCC
CCGCTATGTTTTGAAAAGAGCTTTGGCGGAAGCCAAAGAGCTGGGTTACACCATGTACGTTGGTCCGGAATTAGAGTTTT
TCCTTTTCCAGGTAGATGCAGAAGGAAAACCCACCCTGGTGACCCATGACAATGCCGGCTATTTTGATTTAAGCCCGGTA
GATTTGGGCGAAAACGCCCGGCGGGATATGGTGTTGGCTTTGGAACAAATGGGGTATGAAGTCGAAGCATCCCACCACGA
AGTTGCTCCGGGACAGCACGAAATTGACTTTAAGTATTCCGATGCTTTAGATGTAGCCGATAAAATTGTTACCTTTAAAG
TAGTTGTTCGTTCCATTGCCCAAAGGCACGGACTTCATGCAACCTTCATGCCCAAACCGCTTTTTGGGGTAAATGGTTCG
GGAATGCACACCAACCAGTCGCTGTTTAAAGACGGTCAAAACGCTTTTTATGATCCCAATGGGGAGTTGCAATTATCCGA
AACCGCTTACTACTATATTGGCGGTATTATGAAGCATATTAAAGCTATCACTGCCATTGCCAATCCGGTGGTAAACTCCT
ACAAGCGTTTGGTTCCGGGCTATGAAGCACCGGTTTACATTGCCTGGTCGGCAAGAAACCGTAGCCCCTTAATCCGGATT
CCGGCCAAAAGAGGCTCCTCTACCCGGATTGAATTAAGAAGCCCGGACCCGTCGGCAAATCCGTATCTTGCCTTAGCGGT
TATGCTTAAAGCGGGCTTGGATGGAATTAAAAATAAAATTAAGCCACCGGCGCCGGTTAACCAAAACCTTTATGAAATGA
CTGCTGAAGAGCGAAAGGCTCTGGGAGTTGATACCTTACCCGGTAGCTTAAAAGAAGCCATTGATTGTCTTTTAGAAGAT
AAGGTAATCCAGGAAGCTTTAGGTAAACACGTATTTGAGCGGTTTGTGGAAGCTAAGTTAAAAGAGTGGGATGAGTACCG
GGTTCAGGTTCACGAGTGGGAAGTGAAAAAATATCTCACCATGTATTAA

Upstream 100 bases:

>100_bases
CAAAAGCAAAGCATGGATAAACGGATTTCCATGCGGGCGGTGGCGGAAGCCATCATCTTAGCGTATGACGTTAATCAAAA
AAAATAAAGGGGGATTTTGG

Downstream 100 bases:

>100_bases
AAAGGCTGCCTGATTTAAAGGCAGCCTCTTTTTTTATGTTGTTTTTTAGTATATTATATAGTTAAATTATAGCTATTTTT
TATATCTGGTGAGAAAAATG

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MSYTKNDIMDMVREQNVKFIRLQFTDIFGVLKNVAIPVEQLEKALNNELMFDGSSIEGFVRIEESDMYLRPDPNTFAVFP
WRPRNGAVARLICDVYNPDGTPFEGDPRYVLKRALAEAKELGYTMYVGPELEFFLFQVDAEGKPTLVTHDNAGYFDLSPV
DLGENARRDMVLALEQMGYEVEASHHEVAPGQHEIDFKYSDALDVADKIVTFKVVVRSIAQRHGLHATFMPKPLFGVNGS
GMHTNQSLFKDGQNAFYDPNGELQLSETAYYYIGGIMKHIKAITAIANPVVNSYKRLVPGYEAPVYIAWSARNRSPLIRI
PAKRGSSTRIELRSPDPSANPYLALAVMLKAGLDGIKNKIKPPAPVNQNLYEMTAEERKALGVDTLPGSLKEAIDCLLED
KVIQEALGKHVFERFVEAKLKEWDEYRVQVHEWEVKKYLTMY

Sequences:

>Translated_442_residues
MSYTKNDIMDMVREQNVKFIRLQFTDIFGVLKNVAIPVEQLEKALNNELMFDGSSIEGFVRIEESDMYLRPDPNTFAVFP
WRPRNGAVARLICDVYNPDGTPFEGDPRYVLKRALAEAKELGYTMYVGPELEFFLFQVDAEGKPTLVTHDNAGYFDLSPV
DLGENARRDMVLALEQMGYEVEASHHEVAPGQHEIDFKYSDALDVADKIVTFKVVVRSIAQRHGLHATFMPKPLFGVNGS
GMHTNQSLFKDGQNAFYDPNGELQLSETAYYYIGGIMKHIKAITAIANPVVNSYKRLVPGYEAPVYIAWSARNRSPLIRI
PAKRGSSTRIELRSPDPSANPYLALAVMLKAGLDGIKNKIKPPAPVNQNLYEMTAEERKALGVDTLPGSLKEAIDCLLED
KVIQEALGKHVFERFVEAKLKEWDEYRVQVHEWEVKKYLTMY
>Mature_441_residues
SYTKNDIMDMVREQNVKFIRLQFTDIFGVLKNVAIPVEQLEKALNNELMFDGSSIEGFVRIEESDMYLRPDPNTFAVFPW
RPRNGAVARLICDVYNPDGTPFEGDPRYVLKRALAEAKELGYTMYVGPELEFFLFQVDAEGKPTLVTHDNAGYFDLSPVD
LGENARRDMVLALEQMGYEVEASHHEVAPGQHEIDFKYSDALDVADKIVTFKVVVRSIAQRHGLHATFMPKPLFGVNGSG
MHTNQSLFKDGQNAFYDPNGELQLSETAYYYIGGIMKHIKAITAIANPVVNSYKRLVPGYEAPVYIAWSARNRSPLIRIP
AKRGSSTRIELRSPDPSANPYLALAVMLKAGLDGIKNKIKPPAPVNQNLYEMTAEERKALGVDTLPGSLKEAIDCLLEDK
VIQEALGKHVFERFVEAKLKEWDEYRVQVHEWEVKKYLTMY

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=450, Percent_Identity=24.6666666666667, Blast_Score=145, Evalue=1e-34,
Organism=Escherichia coli, GI1790301, Length=470, Percent_Identity=42.9787234042553, Blast_Score=322, Evalue=2e-89,
Organism=Escherichia coli, GI87081870, Length=459, Percent_Identity=27.4509803921569, Blast_Score=145, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6325292, Length=341, Percent_Identity=27.8592375366569, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 50009; Mature: 49878

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYTKNDIMDMVREQNVKFIRLQFTDIFGVLKNVAIPVEQLEKALNNELMFDGSSIEGFV
CCCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCCCEEE
RIEESDMYLRPDPNTFAVFPWRPRNGAVARLICDVYNPDGTPFEGDPRYVLKRALAEAKE
EEECCCEEECCCCCEEEEEECCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
LGYTMYVGPELEFFLFQVDAEGKPTLVTHDNAGYFDLSPVDLGENARRDMVLALEQMGYE
CCEEEEECCCEEEEEEEECCCCCCEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHCCCE
VEASHHEVAPGQHEIDFKYSDALDVADKIVTFKVVVRSIAQRHGLHATFMPKPLFGVNGS
EECCCCCCCCCCCEECEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCC
GMHTNQSLFKDGQNAFYDPNGELQLSETAYYYIGGIMKHIKAITAIANPVVNSYKRLVPG
CCCCCHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
YEAPVYIAWSARNRSPLIRIPAKRGSSTRIELRSPDPSANPYLALAVMLKAGLDGIKNKI
CCCCEEEEEECCCCCCEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHCHHHHHHCC
KPPAPVNQNLYEMTAEERKALGVDTLPGSLKEAIDCLLEDKVIQEALGKHVFERFVEAKL
CCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEWDEYRVQVHEWEVKKYLTMY
HCCHHHEEEEEEEEHHHHHCCC
>Mature Secondary Structure 
SYTKNDIMDMVREQNVKFIRLQFTDIFGVLKNVAIPVEQLEKALNNELMFDGSSIEGFV
CCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCCCEEE
RIEESDMYLRPDPNTFAVFPWRPRNGAVARLICDVYNPDGTPFEGDPRYVLKRALAEAKE
EEECCCEEECCCCCEEEEEECCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
LGYTMYVGPELEFFLFQVDAEGKPTLVTHDNAGYFDLSPVDLGENARRDMVLALEQMGYE
CCEEEEECCCEEEEEEEECCCCCCEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHCCCE
VEASHHEVAPGQHEIDFKYSDALDVADKIVTFKVVVRSIAQRHGLHATFMPKPLFGVNGS
EECCCCCCCCCCCEECEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCC
GMHTNQSLFKDGQNAFYDPNGELQLSETAYYYIGGIMKHIKAITAIANPVVNSYKRLVPG
CCCCCHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
YEAPVYIAWSARNRSPLIRIPAKRGSSTRIELRSPDPSANPYLALAVMLKAGLDGIKNKI
CCCCEEEEEECCCCCCEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHCHHHHHHCC
KPPAPVNQNLYEMTAEERKALGVDTLPGSLKEAIDCLLEDKVIQEALGKHVFERFVEAKL
CCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEWDEYRVQVHEWEVKKYLTMY
HCCHHHEEEEEEEEHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2891680; 1981087 [H]