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The map label for this gene is glcF [H]

Identifier: 78042735

GI number: 78042735

Start: 375445

End: 376689

Strand: Direct

Name: glcF [H]

Synonym: CHY_0433

Alternate gene names: 78042735

Gene position: 375445-376689 (Clockwise)

Preceding gene: 78043260

Following gene: 78044814

Centisome position: 15.63

GC content: 44.9

Gene sequence:

>1245_bases
GTGACCGAGATTAAACAGGAGTTTTTAAAATGTATGCGTTGCGGTAACTGCCAGGCGGTTTGCCCCATATATAAGGAAAC
AAAAATGGAAGCGGAAGTGGCCCGGGGCAAGATTCAGCTCATCAAAGGGGTTACCGAAGGGGTATTAAAACCTACCGAAT
ACCTGGCGGAAAAAATTGCTACCTGCCTTACCTGTCTTGCCTGCCAGGAAAACTGCCCCAGCGGGGTTAATTATGAGAAA
ATTCTCTTATATGGCAGGGACTATTTGGCCAAAGAAAATGGCTTACCGCCGGTGAAAAAAGCAATCTTTAAAGTTTTACG
CAAACCCCGACTTTTTAATTTTGGCCTGAAAATGGCTTCCCGTTTTCAGGGGCTTGCCCTGAAAAAGGTTAAAGGTCGGC
CGGGAAGGAAACTCAGGTTTGGGCTCCTCGAAGACCATGAAAACCGGTTGTTACCCGAATTTTCCAAGGAAACTCTGGTG
GAAAAGGCCAGGTCTAAACAAACTTCTGGGAAAGAAAAGCTTTTGTATTTCCCCGGCTGTATGATCAATAACGTTTATGT
TAACGTTGGGGAAGCGGTAATTGCTGTTTTGCAGCACAACGGAGCCCAGGTGGTAGTACCGGAAAAGCAAAGCTGCTGTG
GGACTCCCGCCCGGGTGCATGGGGATATAGAGTCGGCCAAGAGCTTAGGACGTTATGTAATTGACACCTATCTTGCGATG
GATGCCGATTACGTTATAACCAGCTGTTCTACCTGCGGGGTAACTTTACGACAGGGATATGTTGAACTTTTTGCCGATGA
GCCGGAATACCTTGAAAAAGCCAAAAAGCTTGCCCAAAAGACCCGGGACATCAATGAATATCTGGTGGAAAAAGGCTTTA
TAAGACCTAAAAAACTAAATTTAAAAGTAACTTACCATGATCCCTGCCATTTAAACCGGAAGCTTAAAGTAAATAAACAA
CCCCGGGAAATATTAAAAGTAGTTTTAGGGGAAAATTTTAGGGAAATGAAAAAGCCTGCTGTCTGTTGCGGCGGGGCCGG
TTCTTTTTCCCTGACCCATTACCCACTCTCGAAAGCTATCGGTAAGAAAAAAGCGTTAGATATCATTGAAACCGGCGCAG
AAGTAGTGGCAACTTCCTGCCCGGGCTGCATGCTCCAGTTAAATGATGCTTTAGCTAAAGAAGGTTCGAATATCAAGGTC
TTACATGTGGCAGAACTTCTGGCGGAAGGATATAAAGAAAATTAA

Upstream 100 bases:

>100_bases
AATGGGGGAAGCGGGGGTTAATCTTTTAAAACGCTTAAAACTTGCTTTTGACCCCAATAATATCTTAAACCCCGGGAAAA
TGGCGTAGGGGGGAAGGTTT

Downstream 100 bases:

>100_bases
TTCACCCCTTAGAAGGATTTAACGGAAAAAGGGAGAAAATATAAAGGCAAAAATTACTTTTGCCGGAGGTTTCGCATGAA
TTTTCAGCAGATAAAAACCA

Product: putative glycolate oxidase, iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MTEIKQEFLKCMRCGNCQAVCPIYKETKMEAEVARGKIQLIKGVTEGVLKPTEYLAEKIATCLTCLACQENCPSGVNYEK
ILLYGRDYLAKENGLPPVKKAIFKVLRKPRLFNFGLKMASRFQGLALKKVKGRPGRKLRFGLLEDHENRLLPEFSKETLV
EKARSKQTSGKEKLLYFPGCMINNVYVNVGEAVIAVLQHNGAQVVVPEKQSCCGTPARVHGDIESAKSLGRYVIDTYLAM
DADYVITSCSTCGVTLRQGYVELFADEPEYLEKAKKLAQKTRDINEYLVEKGFIRPKKLNLKVTYHDPCHLNRKLKVNKQ
PREILKVVLGENFREMKKPAVCCGGAGSFSLTHYPLSKAIGKKKALDIIETGAEVVATSCPGCMLQLNDALAKEGSNIKV
LHVAELLAEGYKEN

Sequences:

>Translated_414_residues
MTEIKQEFLKCMRCGNCQAVCPIYKETKMEAEVARGKIQLIKGVTEGVLKPTEYLAEKIATCLTCLACQENCPSGVNYEK
ILLYGRDYLAKENGLPPVKKAIFKVLRKPRLFNFGLKMASRFQGLALKKVKGRPGRKLRFGLLEDHENRLLPEFSKETLV
EKARSKQTSGKEKLLYFPGCMINNVYVNVGEAVIAVLQHNGAQVVVPEKQSCCGTPARVHGDIESAKSLGRYVIDTYLAM
DADYVITSCSTCGVTLRQGYVELFADEPEYLEKAKKLAQKTRDINEYLVEKGFIRPKKLNLKVTYHDPCHLNRKLKVNKQ
PREILKVVLGENFREMKKPAVCCGGAGSFSLTHYPLSKAIGKKKALDIIETGAEVVATSCPGCMLQLNDALAKEGSNIKV
LHVAELLAEGYKEN
>Mature_413_residues
TEIKQEFLKCMRCGNCQAVCPIYKETKMEAEVARGKIQLIKGVTEGVLKPTEYLAEKIATCLTCLACQENCPSGVNYEKI
LLYGRDYLAKENGLPPVKKAIFKVLRKPRLFNFGLKMASRFQGLALKKVKGRPGRKLRFGLLEDHENRLLPEFSKETLVE
KARSKQTSGKEKLLYFPGCMINNVYVNVGEAVIAVLQHNGAQVVVPEKQSCCGTPARVHGDIESAKSLGRYVIDTYLAMD
ADYVITSCSTCGVTLRQGYVELFADEPEYLEKAKKLAQKTRDINEYLVEKGFIRPKKLNLKVTYHDPCHLNRKLKVNKQP
REILKVVLGENFREMKKPAVCCGGAGSFSLTHYPLSKAIGKKKALDIIETGAEVVATSCPGCMLQLNDALAKEGSNIKVL
HVAELLAEGYKEN

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=395, Percent_Identity=29.1139240506329, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1788576, Length=422, Percent_Identity=26.7772511848341, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786497, Length=234, Percent_Identity=30.3418803418803, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 46132; Mature: 46000

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEIKQEFLKCMRCGNCQAVCPIYKETKMEAEVARGKIQLIKGVTEGVLKPTEYLAEKIA
CCHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TCLTCLACQENCPSGVNYEKILLYGRDYLAKENGLPPVKKAIFKVLRKPRLFNFGLKMAS
HHHHHHHHHHCCCCCCCCEEEEEECCHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHH
RFQGLALKKVKGRPGRKLRFGLLEDHENRLLPEFSKETLVEKARSKQTSGKEKLLYFPGC
HHCCCHHHHCCCCCCCEEEECCHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCE
MINNVYVNVGEAVIAVLQHNGAQVVVPEKQSCCGTPARVHGDIESAKSLGRYVIDTYLAM
EEEEEEEEHHHHHHHHHHCCCCEEEECCCHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHH
DADYVITSCSTCGVTLRQGYVELFADEPEYLEKAKKLAQKTRDINEYLVEKGFIRPKKLN
CCCHHEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
LKVTYHDPCHLNRKLKVNKQPREILKVVLGENFREMKKPAVCCGGAGSFSLTHYPLSKAI
EEEEECCCCCCCCCEECCCCHHHHHHHHHCCCHHHHCCCCEEECCCCCCCEEECCHHHHH
GKKKALDIIETGAEVVATSCPGCMLQLNDALAKEGSNIKVLHVAELLAEGYKEN
CHHHHHHHHHCCHHHHHCCCCCCEEEECHHHHCCCCCEEEEHHHHHHHHHCCCC
>Mature Secondary Structure 
TEIKQEFLKCMRCGNCQAVCPIYKETKMEAEVARGKIQLIKGVTEGVLKPTEYLAEKIA
CHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TCLTCLACQENCPSGVNYEKILLYGRDYLAKENGLPPVKKAIFKVLRKPRLFNFGLKMAS
HHHHHHHHHHCCCCCCCCEEEEEECCHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHH
RFQGLALKKVKGRPGRKLRFGLLEDHENRLLPEFSKETLVEKARSKQTSGKEKLLYFPGC
HHCCCHHHHCCCCCCCEEEECCHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCE
MINNVYVNVGEAVIAVLQHNGAQVVVPEKQSCCGTPARVHGDIESAKSLGRYVIDTYLAM
EEEEEEEEHHHHHHHHHHCCCCEEEECCCHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHH
DADYVITSCSTCGVTLRQGYVELFADEPEYLEKAKKLAQKTRDINEYLVEKGFIRPKKLN
CCCHHEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
LKVTYHDPCHLNRKLKVNKQPREILKVVLGENFREMKKPAVCCGGAGSFSLTHYPLSKAI
EEEEECCCCCCCCCEECCCCHHHHHHHHHCCCHHHHCCCCEEECCCCCCCEEECCHHHHH
GKKKALDIIETGAEVVATSCPGCMLQLNDALAKEGSNIKVLHVAELLAEGYKEN
CHHHHHHHHHCCHHHHHCCCCCCEEEECHHHHCCCCCEEEEHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]