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The map label for this gene is glcD [H]

Identifier: 78043260

GI number: 78043260

Start: 374047

End: 375432

Strand: Direct

Name: glcD [H]

Synonym: CHY_0432

Alternate gene names: 78043260

Gene position: 374047-375432 (Clockwise)

Preceding gene: 78044577

Following gene: 78042735

Centisome position: 15.58

GC content: 45.67

Gene sequence:

>1386_bases
TTGAGGAGGTTTTTTAAAGTGGAAGCACTGGCGAAGCTACAAAAAATTCTTGGTTCCGGGAAGATAAAAACGCAACCGGA
GGAGCTTTTTGTTTACGGTTATGACGCTACCGCGGGGTTAAAAAACCAAATGCCTTTAGCGGTGGTATTTCCTGAAAGCA
CCGAGGAGGTAGTCGAGATTGTTAAATGGGCCAACGAATATAAAATTCCTCTTTATCCGCGGGGTTCAGGGACAAATTTA
AGCGGTGGAACTGTGCCGACGGCAAAAGGGGTAGTGGTAGAGTTAAATCGTCTCAATAAAATCTTAGAGATTGACCTGGA
TAACCTTACGGCTACCGTGGAGCCGGGGGTAATCATCAACGATTTAAATGAAGCGGTGAAACCCTACGGCTTGATTTATC
CGCCGGACCCGGGAACGGTTACTACTGCTACTATGGGCGGTTCGGTGGCCGAGTGTTCCGGTGGGCTTCGGGGCCTAAAA
TACGGTGTAACCAAGCATTATATCATGGGCGTCGAAGCGGTAATTGGTACTGGTGAACTGTTGAAATTTGGCGGCAAGAC
CGTGAAAAATGTTACCGGCTATGATCTGCCTGCGTTAATGGTGGGAAGCGAAGGTACCTTAGGGATTATTACGAAAATTA
TCGTAAAACTTATTCCCGCACCGGTAGCCAAAAAAAGCTTCTTAGCTGTATTCAACAGTATTGATGATGCTGGGAACGCC
ATTGCTGAAATTATTAAAAACCGGGTTATTCCTGCAACTTTAGAAATTATGGACCAAACAACCATCCGGACGGTAGAAAA
ATTTAAAAACATTGGCCTTCCGGTAGATGCCCAGGCGATTTTACTGGTGGAAACCGATGGTTATCCCGAACAGGTGGAAA
TGGAAGCTAAAATTATTCGGCAGGTCCTGGAAAAAAATCGGGGAGAAGTATCTGAAGCCAAAAATGATGAAGAGCGGGAA
AAACTCTGGGAAGCACGGAGGGCGGCATTACCGGCTTTAGCCCAGGTAAGTCCCACCACCGTTTTGGAAGATGCTACAGT
TCCCCGGAGTCAAGTTCCGGCTATGTTAAAGAGGTTAAAGGAAATTAGTGAAAAATATAATCTTACTATTGGTACTTTTG
GTCACGCAGGTGATGGAAATCTTCATCCTACAATCCTAACCGACGAAACTAACCGGGAAGAATGGCAGCGAGTGGAAAAA
GCTGTAGAAGAAATCTTTAAAGCGGCGTTAGAACTTGGAGGAACGCTTTCCGGGGAACACGGCATTGGCATGGCGAAAAA
CCGTTTTCTCCTCTGGGAAATGGGGGAAGCGGGGGTTAATCTTTTAAAACGCTTAAAACTTGCTTTTGACCCCAATAATA
TCTTAAACCCCGGGAAAATGGCGTAG

Upstream 100 bases:

>100_bases
CGCAGTGGTAATTTTAGCCCTTTGGGGCTTAGTTGCAGCCCTTAATAAAAAATAAATTAAAATAAAACTAAGCGGGGGGA
GCTATTCCCCCCGGATAGTT

Downstream 100 bases:

>100_bases
GGGGGAAGGTTTGTGACCGAGATTAAACAGGAGTTTTTAAAATGTATGCGTTGCGGTAACTGCCAGGCGGTTTGCCCCAT
ATATAAGGAAACAAAAATGG

Product: putative glycolate oxidase, GlcD subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MRRFFKVEALAKLQKILGSGKIKTQPEELFVYGYDATAGLKNQMPLAVVFPESTEEVVEIVKWANEYKIPLYPRGSGTNL
SGGTVPTAKGVVVELNRLNKILEIDLDNLTATVEPGVIINDLNEAVKPYGLIYPPDPGTVTTATMGGSVAECSGGLRGLK
YGVTKHYIMGVEAVIGTGELLKFGGKTVKNVTGYDLPALMVGSEGTLGIITKIIVKLIPAPVAKKSFLAVFNSIDDAGNA
IAEIIKNRVIPATLEIMDQTTIRTVEKFKNIGLPVDAQAILLVETDGYPEQVEMEAKIIRQVLEKNRGEVSEAKNDEERE
KLWEARRAALPALAQVSPTTVLEDATVPRSQVPAMLKRLKEISEKYNLTIGTFGHAGDGNLHPTILTDETNREEWQRVEK
AVEEIFKAALELGGTLSGEHGIGMAKNRFLLWEMGEAGVNLLKRLKLAFDPNNILNPGKMA

Sequences:

>Translated_461_residues
MRRFFKVEALAKLQKILGSGKIKTQPEELFVYGYDATAGLKNQMPLAVVFPESTEEVVEIVKWANEYKIPLYPRGSGTNL
SGGTVPTAKGVVVELNRLNKILEIDLDNLTATVEPGVIINDLNEAVKPYGLIYPPDPGTVTTATMGGSVAECSGGLRGLK
YGVTKHYIMGVEAVIGTGELLKFGGKTVKNVTGYDLPALMVGSEGTLGIITKIIVKLIPAPVAKKSFLAVFNSIDDAGNA
IAEIIKNRVIPATLEIMDQTTIRTVEKFKNIGLPVDAQAILLVETDGYPEQVEMEAKIIRQVLEKNRGEVSEAKNDEERE
KLWEARRAALPALAQVSPTTVLEDATVPRSQVPAMLKRLKEISEKYNLTIGTFGHAGDGNLHPTILTDETNREEWQRVEK
AVEEIFKAALELGGTLSGEHGIGMAKNRFLLWEMGEAGVNLLKRLKLAFDPNNILNPGKMA
>Mature_461_residues
MRRFFKVEALAKLQKILGSGKIKTQPEELFVYGYDATAGLKNQMPLAVVFPESTEEVVEIVKWANEYKIPLYPRGSGTNL
SGGTVPTAKGVVVELNRLNKILEIDLDNLTATVEPGVIINDLNEAVKPYGLIYPPDPGTVTTATMGGSVAECSGGLRGLK
YGVTKHYIMGVEAVIGTGELLKFGGKTVKNVTGYDLPALMVGSEGTLGIITKIIVKLIPAPVAKKSFLAVFNSIDDAGNA
IAEIIKNRVIPATLEIMDQTTIRTVEKFKNIGLPVDAQAILLVETDGYPEQVEMEAKIIRQVLEKNRGEVSEAKNDEERE
KLWEARRAALPALAQVSPTTVLEDATVPRSQVPAMLKRLKEISEKYNLTIGTFGHAGDGNLHPTILTDETNREEWQRVEK
AVEEIFKAALELGGTLSGEHGIGMAKNRFLLWEMGEAGVNLLKRLKLAFDPNNILNPGKMA

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=454, Percent_Identity=32.3788546255507, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI37595754, Length=477, Percent_Identity=30.8176100628931, Blast_Score=238, Evalue=7e-63,
Organism=Homo sapiens, GI119964728, Length=472, Percent_Identity=27.3305084745763, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI4501993, Length=470, Percent_Identity=24.8936170212766, Blast_Score=121, Evalue=2e-27,
Organism=Escherichia coli, GI1789351, Length=455, Percent_Identity=37.5824175824176, Blast_Score=326, Evalue=2e-90,
Organism=Escherichia coli, GI1787977, Length=495, Percent_Identity=24.4444444444444, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI48994907, Length=444, Percent_Identity=25.2252252252252, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI48994914, Length=193, Percent_Identity=33.160621761658, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71992373, Length=475, Percent_Identity=29.6842105263158, Blast_Score=227, Evalue=9e-60,
Organism=Caenorhabditis elegans, GI71992381, Length=475, Percent_Identity=28.4210526315789, Blast_Score=207, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17534361, Length=425, Percent_Identity=30.1176470588235, Blast_Score=182, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI71992392, Length=360, Percent_Identity=29.7222222222222, Blast_Score=181, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17556096, Length=458, Percent_Identity=24.235807860262, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6320027, Length=430, Percent_Identity=31.6279069767442, Blast_Score=217, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6320023, Length=484, Percent_Identity=29.9586776859504, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6320764, Length=487, Percent_Identity=28.7474332648871, Blast_Score=182, Evalue=8e-47,
Organism=Drosophila melanogaster, GI18921117, Length=459, Percent_Identity=29.8474945533769, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24639277, Length=459, Percent_Identity=29.8474945533769, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24639275, Length=459, Percent_Identity=29.8474945533769, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24653753, Length=465, Percent_Identity=23.8709677419355, Blast_Score=125, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 50187; Mature: 50187

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRFFKVEALAKLQKILGSGKIKTQPEELFVYGYDATAGLKNQMPLAVVFPESTEEVVEI
CCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCHHHHHHH
VKWANEYKIPLYPRGSGTNLSGGTVPTAKGVVVELNRLNKILEIDLDNLTATVEPGVIIN
HHHCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEHHHCCCEEEEECCCCEEEECCCEEEE
DLNEAVKPYGLIYPPDPGTVTTATMGGSVAECSGGLRGLKYGVTKHYIMGVEAVIGTGEL
CHHHHHCCCEEEECCCCCCEEEEECCCCHHHHCCCHHHHHHCCHHHHHHHHHHHHCCCHH
LKFGGKTVKNVTGYDLPALMVGSEGTLGIITKIIVKLIPAPVAKKSFLAVFNSIDDAGNA
HHHCCCHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHH
IAEIIKNRVIPATLEIMDQTTIRTVEKFKNIGLPVDAQAILLVETDGYPEQVEMEAKIIR
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHH
QVLEKNRGEVSEAKNDEEREKLWEARRAALPALAQVSPTTVLEDATVPRSQVPAMLKRLK
HHHHCCCCCHHHCCCHHHHHHHHHHHHHHCCHHHHCCCCCEECCCCCCHHHHHHHHHHHH
EISEKYNLTIGTFGHAGDGNLHPTILTDETNREEWQRVEKAVEEIFKAALELGGTLSGEH
HHHHHHCEEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GIGMAKNRFLLWEMGEAGVNLLKRLKLAFDPNNILNPGKMA
CCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRRFFKVEALAKLQKILGSGKIKTQPEELFVYGYDATAGLKNQMPLAVVFPESTEEVVEI
CCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCHHHHHHH
VKWANEYKIPLYPRGSGTNLSGGTVPTAKGVVVELNRLNKILEIDLDNLTATVEPGVIIN
HHHCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEHHHCCCEEEEECCCCEEEECCCEEEE
DLNEAVKPYGLIYPPDPGTVTTATMGGSVAECSGGLRGLKYGVTKHYIMGVEAVIGTGEL
CHHHHHCCCEEEECCCCCCEEEEECCCCHHHHCCCHHHHHHCCHHHHHHHHHHHHCCCHH
LKFGGKTVKNVTGYDLPALMVGSEGTLGIITKIIVKLIPAPVAKKSFLAVFNSIDDAGNA
HHHCCCHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHH
IAEIIKNRVIPATLEIMDQTTIRTVEKFKNIGLPVDAQAILLVETDGYPEQVEMEAKIIR
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHH
QVLEKNRGEVSEAKNDEEREKLWEARRAALPALAQVSPTTVLEDATVPRSQVPAMLKRLK
HHHHCCCCCHHHCCCHHHHHHHHHHHHHHCCHHHHCCCCCEECCCCCCHHHHHHHHHHHH
EISEKYNLTIGTFGHAGDGNLHPTILTDETNREEWQRVEKAVEEIFKAALELGGTLSGEH
HHHHHHCEEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GIGMAKNRFLLWEMGEAGVNLLKRLKLAFDPNNILNPGKMA
CCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]