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The map label for this gene is alkK [H]

Identifier: 78042621

GI number: 78042621

Start: 510321

End: 511919

Strand: Direct

Name: alkK [H]

Synonym: CHY_0593

Alternate gene names: 78042621

Gene position: 510321-511919 (Clockwise)

Preceding gene: 78045093

Following gene: 78043303

Centisome position: 21.25

GC content: 43.09

Gene sequence:

>1599_bases
ATGAAAGGTTTTGGTTTTATGAACTTTCAGTTAAATTTGGGGAAGATATTAAAGCGCGCTGCCAGCTACTTTGGGGAAAA
AACCATCGTATCTTATGAAGCTCTGGAGCGAAAGGAGATAACTTATAGAGAGTTTTACTCCCGGGTCTTGGCTTTAATTG
GTTATTTAAAAGCTCTGGGGGTTAAAAGAGGAAGTCGGGTAGGGGTTTTTGCCTGGAATAATTTAGCCCATTTAATGCTT
TATTATGCGGTGCCTTGCTCCGAAGCAGTTTTACACCCGGTAAATTTACGGTACAGCAAAGAGCAAATGCTTTATACCAT
AAATCACGCTGAAGATGAAGTTTTATTTGTGGAAAAGGAATTTTTGCCTTTAGTGGCGGAAATAAAAAGCGAACTTCAGA
GAGTGCAACAAATATTACCTTTAGACGCGGTTTTTACAAATTTTGTTTCTTTTGCCGAAAATTTTGACCAAATTCCGGAA
GACCTTCCGGCAAAATTATGTTATACCACCGCAACCACCGGGACTCCCAAAGGCGTAATGTATTCTCACCGGGACCTGTA
TCTGCAAAGCATGGCTCTTTGCATGACCGATAGTTTTGGCATATCGGAGAAAGAACGAATATTAATCATGGTTCCGATGT
TTCACGTAAATTCCTGGGGAATTCCTTATGCTGCGGCAATGGTAGGTGCTACGATGGTCTTACCCGGGGGAAATTTTAAA
GGGCAATTTTTAGCTAAAATAATTTCTGAAGAAAAAGTAAGTTTAGCTGCAGGAGTACCTACCGTTTTCCAGGAAATTTT
AAAAGCAGCGCAAAGCGAAAATATTGATTTGGGAAGCCTGCGAACTGTTCTGGTGGGTGGAGCTCCCCTTACCAGAGAAA
TTATTGAGGGATTTGCCCGATATGGTGTTGAAGTTCGTCAGGTCTATGGACTTACCGAAACCGCGCCTTTTGTAGCAAGT
AATTACCAGAAATCAACCCTCGTCCATCTTAGCGAAGAAGAAAAGAAAAAACAGCAGCTAAAAATCGGCCTTCCCGCTCC
TGGCGTTGAGGTAAGGGTGGTGGGCAAAGATGGAAAGGATGTGCCCTGGGATGGGGAATCCATTGGCGAGCTCTGGTTAA
AAGGTCCCTGGCTTGCCCGGGAATATTACAATGATGAAAAGCATACCCGGGAAGCTTTTGTGGATGGCTGGTTTAGAACT
TTTGATTTAGTTAAAATTGATGCGTTAGGGTATATTGAGTTTTGCGACCGGGAAAAAGATGTAATAAAAAGCGGTGGGGA
ATGGATTTCCTCGGTGGCGGTGGAGAAATATCTTCTGTCCCACCCCGATGTAAAAGAAGCGGCGGTGGTAGGTTTACCCC
ATCCCCTCTGGCAGGAACGACCGGTAGCCTTTGTGACTCTATGGGAACAGGCAAAGGTAACCGAAGAAGAACTTCTAAGT
TATTTGCGGAGTAAACTTCTTTCCTTCTGGGTTCCCGACCGCATACTTTTTTTACGGGAGTTGCCCCGTAATTCGGTAGG
TAAAGTAGCCAAAAAATTACTGCGGGAAAAATATCATGATTTATTGCTTAAAAACCCGAACGAAAGGGAAAAAGTATAA

Upstream 100 bases:

>100_bases
TTCTCAATTTTGTTTAACTTGATACTTAATAACATTTTTCTATCCATTTTAACACCCCAAGGAAATTGTAACATAAATTT
ACAAAATTTACAATATTATA

Downstream 100 bases:

>100_bases
AAGTATAAAAGCAGATTTTTAATGGGAATAATACCATACTAAGTGGTGCCAATTTACCCGTTGCTTTTTTAAAATCACCG
GTATATACCGGTGATTTAAT

Product: medium-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MKGFGFMNFQLNLGKILKRAASYFGEKTIVSYEALERKEITYREFYSRVLALIGYLKALGVKRGSRVGVFAWNNLAHLML
YYAVPCSEAVLHPVNLRYSKEQMLYTINHAEDEVLFVEKEFLPLVAEIKSELQRVQQILPLDAVFTNFVSFAENFDQIPE
DLPAKLCYTTATTGTPKGVMYSHRDLYLQSMALCMTDSFGISEKERILIMVPMFHVNSWGIPYAAAMVGATMVLPGGNFK
GQFLAKIISEEKVSLAAGVPTVFQEILKAAQSENIDLGSLRTVLVGGAPLTREIIEGFARYGVEVRQVYGLTETAPFVAS
NYQKSTLVHLSEEEKKKQQLKIGLPAPGVEVRVVGKDGKDVPWDGESIGELWLKGPWLAREYYNDEKHTREAFVDGWFRT
FDLVKIDALGYIEFCDREKDVIKSGGEWISSVAVEKYLLSHPDVKEAAVVGLPHPLWQERPVAFVTLWEQAKVTEEELLS
YLRSKLLSFWVPDRILFLRELPRNSVGKVAKKLLREKYHDLLLKNPNEREKV

Sequences:

>Translated_532_residues
MKGFGFMNFQLNLGKILKRAASYFGEKTIVSYEALERKEITYREFYSRVLALIGYLKALGVKRGSRVGVFAWNNLAHLML
YYAVPCSEAVLHPVNLRYSKEQMLYTINHAEDEVLFVEKEFLPLVAEIKSELQRVQQILPLDAVFTNFVSFAENFDQIPE
DLPAKLCYTTATTGTPKGVMYSHRDLYLQSMALCMTDSFGISEKERILIMVPMFHVNSWGIPYAAAMVGATMVLPGGNFK
GQFLAKIISEEKVSLAAGVPTVFQEILKAAQSENIDLGSLRTVLVGGAPLTREIIEGFARYGVEVRQVYGLTETAPFVAS
NYQKSTLVHLSEEEKKKQQLKIGLPAPGVEVRVVGKDGKDVPWDGESIGELWLKGPWLAREYYNDEKHTREAFVDGWFRT
FDLVKIDALGYIEFCDREKDVIKSGGEWISSVAVEKYLLSHPDVKEAAVVGLPHPLWQERPVAFVTLWEQAKVTEEELLS
YLRSKLLSFWVPDRILFLRELPRNSVGKVAKKLLREKYHDLLLKNPNEREKV
>Mature_532_residues
MKGFGFMNFQLNLGKILKRAASYFGEKTIVSYEALERKEITYREFYSRVLALIGYLKALGVKRGSRVGVFAWNNLAHLML
YYAVPCSEAVLHPVNLRYSKEQMLYTINHAEDEVLFVEKEFLPLVAEIKSELQRVQQILPLDAVFTNFVSFAENFDQIPE
DLPAKLCYTTATTGTPKGVMYSHRDLYLQSMALCMTDSFGISEKERILIMVPMFHVNSWGIPYAAAMVGATMVLPGGNFK
GQFLAKIISEEKVSLAAGVPTVFQEILKAAQSENIDLGSLRTVLVGGAPLTREIIEGFARYGVEVRQVYGLTETAPFVAS
NYQKSTLVHLSEEEKKKQQLKIGLPAPGVEVRVVGKDGKDVPWDGESIGELWLKGPWLAREYYNDEKHTREAFVDGWFRT
FDLVKIDALGYIEFCDREKDVIKSGGEWISSVAVEKYLLSHPDVKEAAVVGLPHPLWQERPVAFVTLWEQAKVTEEELLS
YLRSKLLSFWVPDRILFLRELPRNSVGKVAKKLLREKYHDLLLKNPNEREKV

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=530, Percent_Identity=29.0566037735849, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI187761343, Length=530, Percent_Identity=29.0566037735849, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI156151445, Length=554, Percent_Identity=24.9097472924188, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI28416953, Length=528, Percent_Identity=25.9469696969697, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI122937307, Length=485, Percent_Identity=24.5360824742268, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI13375727, Length=545, Percent_Identity=24.0366972477064, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI42544132, Length=494, Percent_Identity=22.4696356275304, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI115511026, Length=378, Percent_Identity=25.3968253968254, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI157311624, Length=493, Percent_Identity=23.9350912778905, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI157311622, Length=493, Percent_Identity=23.9350912778905, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI38505220, Length=457, Percent_Identity=22.5382932166302, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI58082049, Length=493, Percent_Identity=23.5294117647059, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI253970489, Length=384, Percent_Identity=27.34375, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI8923896, Length=383, Percent_Identity=26.8929503916449, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788107, Length=518, Percent_Identity=26.4478764478764, Blast_Score=162, Evalue=5e-41,
Organism=Escherichia coli, GI145693145, Length=512, Percent_Identity=25.1953125, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI221142682, Length=519, Percent_Identity=23.121387283237, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1786810, Length=378, Percent_Identity=30.952380952381, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1790505, Length=568, Percent_Identity=23.4154929577465, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788595, Length=485, Percent_Identity=24.3298969072165, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789201, Length=500, Percent_Identity=22.4, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786801, Length=428, Percent_Identity=21.9626168224299, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1786529, Length=543, Percent_Identity=25.0460405156538, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17558820, Length=498, Percent_Identity=28.1124497991968, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI32563687, Length=493, Percent_Identity=27.9918864097363, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17560308, Length=549, Percent_Identity=26.5938069216758, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI71994703, Length=526, Percent_Identity=26.8060836501901, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71994690, Length=526, Percent_Identity=26.8060836501901, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71994694, Length=526, Percent_Identity=26.8060836501901, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17559526, Length=507, Percent_Identity=24.2603550295858, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17531443, Length=514, Percent_Identity=24.7081712062257, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17557194, Length=519, Percent_Identity=24.6628131021195, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17560140, Length=356, Percent_Identity=26.123595505618, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71985884, Length=367, Percent_Identity=25.8855585831063, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17538037, Length=518, Percent_Identity=23.9382239382239, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71996755, Length=495, Percent_Identity=23.8383838383838, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17510401, Length=485, Percent_Identity=21.2371134020619, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6319699, Length=359, Percent_Identity=26.1838440111421, Blast_Score=126, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6319264, Length=567, Percent_Identity=23.6331569664903, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323182, Length=578, Percent_Identity=23.356401384083, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6320852, Length=368, Percent_Identity=26.6304347826087, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24581924, Length=555, Percent_Identity=27.3873873873874, Blast_Score=169, Evalue=4e-42,
Organism=Drosophila melanogaster, GI21355181, Length=530, Percent_Identity=27.7358490566038, Blast_Score=169, Evalue=4e-42,
Organism=Drosophila melanogaster, GI18859661, Length=361, Percent_Identity=26.3157894736842, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24648253, Length=508, Percent_Identity=22.8346456692913, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648255, Length=508, Percent_Identity=23.2283464566929, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21356947, Length=537, Percent_Identity=22.9050279329609, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI19922652, Length=535, Percent_Identity=20.7476635514019, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24648260, Length=543, Percent_Identity=23.3885819521179, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI20130357, Length=502, Percent_Identity=22.9083665338645, Blast_Score=93, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21355225, Length=532, Percent_Identity=23.3082706766917, Blast_Score=93, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24653035, Length=527, Percent_Identity=19.9240986717268, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356441, Length=480, Percent_Identity=21.6666666666667, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI62472339, Length=372, Percent_Identity=25.8064516129032, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24667955, Length=372, Percent_Identity=25.8064516129032, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24667959, Length=372, Percent_Identity=25.8064516129032, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI161076582, Length=535, Percent_Identity=19.6261682242991, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21358303, Length=474, Percent_Identity=20.6751054852321, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281365686, Length=544, Percent_Identity=19.1176470588235, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648257, Length=537, Percent_Identity=21.6014897579143, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 60352; Mature: 60352

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGFGFMNFQLNLGKILKRAASYFGEKTIVSYEALERKEITYREFYSRVLALIGYLKALG
CCCCEEEEEEECHHHHHHHHHHHCCCHHEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHC
VKRGSRVGVFAWNNLAHLMLYYAVPCSEAVLHPVNLRYSKEQMLYTINHAEDEVLFVEKE
CCCCCEEEEEEECHHHHHHHHHHCCCCHHHCCCCCCCCCCCCEEEEEECCCCCEEEEEHH
FLPLVAEIKSELQRVQQILPLDAVFTNFVSFAENFDQIPEDLPAKLCYTTATTGTPKGVM
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHCCCCEEEEECCCCCCCCCE
YSHRDLYLQSMALCMTDSFGISEKERILIMVPMFHVNSWGIPYAAAMVGATMVLPGGNFK
ECHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHCCEEEECCCCCC
GQFLAKIISEEKVSLAAGVPTVFQEILKAAQSENIDLGSLRTVLVGGAPLTREIIEGFAR
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHEEEECCCCHHHHHHHHHHH
YGVEVRQVYGLTETAPFVASNYQKSTLVHLSEEEKKKQQLKIGLPAPGVEVRVVGKDGKD
CCCHHHHHHCCCCCCCHHHCCCCCCCEEECCHHHHHHHHEEECCCCCCCEEEEECCCCCC
VPWDGESIGELWLKGPWLAREYYNDEKHTREAFVDGWFRTFDLVKIDALGYIEFCDREKD
CCCCCCHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHEEEEECCCHHHHHCCCHH
VIKSGGEWISSVAVEKYLLSHPDVKEAAVVGLPHPLWQERPVAFVTLWEQAKVTEEELLS
HHHCCHHHHHHHHHHHHHHCCCCHHHHHEECCCCCCCCCCCEEEEEECCHHCCCHHHHHH
YLRSKLLSFWVPDRILFLRELPRNSVGKVAKKLLREKYHDLLLKNPNEREKV
HHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKGFGFMNFQLNLGKILKRAASYFGEKTIVSYEALERKEITYREFYSRVLALIGYLKALG
CCCCEEEEEEECHHHHHHHHHHHCCCHHEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHC
VKRGSRVGVFAWNNLAHLMLYYAVPCSEAVLHPVNLRYSKEQMLYTINHAEDEVLFVEKE
CCCCCEEEEEEECHHHHHHHHHHCCCCHHHCCCCCCCCCCCCEEEEEECCCCCEEEEEHH
FLPLVAEIKSELQRVQQILPLDAVFTNFVSFAENFDQIPEDLPAKLCYTTATTGTPKGVM
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHCCCCEEEEECCCCCCCCCE
YSHRDLYLQSMALCMTDSFGISEKERILIMVPMFHVNSWGIPYAAAMVGATMVLPGGNFK
ECHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHCCEEEECCCCCC
GQFLAKIISEEKVSLAAGVPTVFQEILKAAQSENIDLGSLRTVLVGGAPLTREIIEGFAR
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHEEEECCCCHHHHHHHHHHH
YGVEVRQVYGLTETAPFVASNYQKSTLVHLSEEEKKKQQLKIGLPAPGVEVRVVGKDGKD
CCCHHHHHHCCCCCCCHHHCCCCCCCEEECCHHHHHHHHEEECCCCCCCEEEEECCCCCC
VPWDGESIGELWLKGPWLAREYYNDEKHTREAFVDGWFRTFDLVKIDALGYIEFCDREKD
CCCCCCHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHEEEEECCCHHHHHCCCHH
VIKSGGEWISSVAVEKYLLSHPDVKEAAVVGLPHPLWQERPVAFVTLWEQAKVTEEELLS
HHHCCHHHHHHHHHHHHHHCCCCHHHHHEECCCCCCCCCCCEEEEEECCHHCCCHHHHHH
YLRSKLLSFWVPDRILFLRELPRNSVGKVAKKLLREKYHDLLLKNPNEREKV
HHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]