Click here to switch to the map view.

The map label for this gene is cysP [H]

Identifier: 77465705

GI number: 77465705

Start: 818296

End: 819288

Strand: Direct

Name: cysP [H]

Synonym: RSP_3697

Alternate gene names: 77465705

Gene position: 818296-819288 (Clockwise)

Preceding gene: 77465704

Following gene: 77465706

Centisome position: 86.77

GC content: 65.26

Gene sequence:

>993_bases
GTGAAAAGCCCCATCCTCGCCGCGATCCTGGCCCTCGGCCTCGCCGCCCCCGCCGCCGCGCAGGACAAGATCCTGAACGT
CTCCTACGACATCGCCCGCGAGCTGTTCGAGGCGATCAACCCCGCCTTCGCCGAGAAATGGAAGGCCGACACCGGCCGCG
ACGTCACCATCGACCAGTCGCACGGCGGCTCGTCCAAGCAGGCGCGCGCCATCCTCGAGGGGCTGCCGGCGGATGTGGTC
ACCTTCAATCAGGTGACGGACATCGACGTGCTGGCCGAGGCCGGCATGGTGGACGAAAACTGGCGCGAGGCCTTCCCGAA
CGCGGCCTCGCCCTATTACTCGCTGCCGGCCTTCCTCGTGCGAAAGGACAATCCCAAGGGGATCGAGGACTGGGACGATC
TGGTGAAGGAAGGCGTCGAGGTCATCTTCCCGAATCCCAAGACCAGCGGCAATGCGCGCTACACCTATCTCGCGGCCTAT
GCCTACGCGCTGGAGGCGAACGGCGGCGATCAGGCCAAGGCGCAGGAGTTCGTGAAGCAGCTCTTCGCCAATGTGCCCGT
CTTCGATCAGGGCGGCCGCGCGGCCACCGTGACCTTTGCCGAGAAGGGCATCGGCGACGTGCTCATCACCTTCGAGGCCG
AGACGGGGGGCATCGCCGCGCAATATGCCGATCAGGGCCTCGAGCGGGTCACGCCCTCGCTCTCGCTCTATTCCGAGTTC
CCGGTCGCCATCGTGACCGAGAATGCCGAGCGCAACGGCTCGGCCGAGGTCTCCAAGTCCTATCTCGACTTCCTCTATGC
GCCCGAGGGGCAGAAGATCCTCGCCGAACATTTCTACCGGGTGCATGACGAGACGGCACAGGCCGCCTTCGCCGCGAACT
TCCCCGAGGTGAAGCTCGTCGATGTCGACGAGACCTTCGGCGGCTGGGAGAAGATCAAGGCCGAGCATTTCGCCGAGGGC
GGCATCCTCGACACGGTCTTCGTGAACCAATGA

Upstream 100 bases:

>100_bases
AGGCCGGCCAGATCTTCCCCGGCCAGACCCATCTCGGAGGCGACACCGCTGCCGCGGCCGCCCCCTCCGCCGACCGACCG
ATCCTGAAGGAGTCCCACCT

Downstream 100 bases:

>100_bases
TCGCGCGCACCGCACCCGGCCCGGCCCGCCGGTCGAAACGGGTCCTGCCCGGCTTCGGCCTGACCATGGGCATGGTGCTG
ACCTATCTGGGCGTCATCGT

Product: ABC sulfate/thiosulfate transporter, periplasmic binding protein CysP

Products: SO42- [Cytoplasm]; phosphate; ADP; S2O32- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKSPILAAILALGLAAPAAAQDKILNVSYDIARELFEAINPAFAEKWKADTGRDVTIDQSHGGSSKQARAILEGLPADVV
TFNQVTDIDVLAEAGMVDENWREAFPNAASPYYSLPAFLVRKDNPKGIEDWDDLVKEGVEVIFPNPKTSGNARYTYLAAY
AYALEANGGDQAKAQEFVKQLFANVPVFDQGGRAATVTFAEKGIGDVLITFEAETGGIAAQYADQGLERVTPSLSLYSEF
PVAIVTENAERNGSAEVSKSYLDFLYAPEGQKILAEHFYRVHDETAQAAFAANFPEVKLVDVDETFGGWEKIKAEHFAEG
GILDTVFVNQ

Sequences:

>Translated_330_residues
MKSPILAAILALGLAAPAAAQDKILNVSYDIARELFEAINPAFAEKWKADTGRDVTIDQSHGGSSKQARAILEGLPADVV
TFNQVTDIDVLAEAGMVDENWREAFPNAASPYYSLPAFLVRKDNPKGIEDWDDLVKEGVEVIFPNPKTSGNARYTYLAAY
AYALEANGGDQAKAQEFVKQLFANVPVFDQGGRAATVTFAEKGIGDVLITFEAETGGIAAQYADQGLERVTPSLSLYSEF
PVAIVTENAERNGSAEVSKSYLDFLYAPEGQKILAEHFYRVHDETAQAAFAANFPEVKLVDVDETFGGWEKIKAEHFAEG
GILDTVFVNQ
>Mature_330_residues
MKSPILAAILALGLAAPAAAQDKILNVSYDIARELFEAINPAFAEKWKADTGRDVTIDQSHGGSSKQARAILEGLPADVV
TFNQVTDIDVLAEAGMVDENWREAFPNAASPYYSLPAFLVRKDNPKGIEDWDDLVKEGVEVIFPNPKTSGNARYTYLAAY
AYALEANGGDQAKAQEFVKQLFANVPVFDQGGRAATVTFAEKGIGDVLITFEAETGGIAAQYADQGLERVTPSLSLYSEF
PVAIVTENAERNGSAEVSKSYLDFLYAPEGQKILAEHFYRVHDETAQAAFAANFPEVKLVDVDETFGGWEKIKAEHFAEG
GILDTVFVNQ

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. This protein specifically binds thiosulfate and is involved in its transmembrane transport [H]

COG id: COG4150

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1788765, Length=307, Percent_Identity=51.1400651465798, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1790351, Length=313, Percent_Identity=49.5207667731629, Blast_Score=316, Evalue=2e-87,

Paralogues:

None

Copy number: 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 35812; Mature: 35812

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSPILAAILALGLAAPAAAQDKILNVSYDIARELFEAINPAFAEKWKADTGRDVTIDQS
CCCHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHCHHHHHHHCCCCCCEEEEECC
HGGSSKQARAILEGLPADVVTFNQVTDIDVLAEAGMVDENWREAFPNAASPYYSLPAFLV
CCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHCCCCCCHHHHHCCCCCCCCCCCCEEEE
RKDNPKGIEDWDDLVKEGVEVIFPNPKTSGNARYTYLAAYAYALEANGGDQAKAQEFVKQ
ECCCCCCCHHHHHHHHCCCEEEECCCCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHH
LFANVPVFDQGGRAATVTFAEKGIGDVLITFEAETGGIAAQYADQGLERVTPSLSLYSEF
HHHCCCEECCCCCEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHCCC
PVAIVTENAERNGSAEVSKSYLDFLYAPEGQKILAEHFYRVHDETAQAAFAANFPEVKLV
CEEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
DVDETFGGWEKIKAEHFAEGGILDTVFVNQ
ECCCCCCCHHHHHHHHHCCCCCEEEEEECC
>Mature Secondary Structure
MKSPILAAILALGLAAPAAAQDKILNVSYDIARELFEAINPAFAEKWKADTGRDVTIDQS
CCCHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHCHHHHHHHCCCCCCEEEEECC
HGGSSKQARAILEGLPADVVTFNQVTDIDVLAEAGMVDENWREAFPNAASPYYSLPAFLV
CCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHCCCCCCHHHHHCCCCCCCCCCCCEEEE
RKDNPKGIEDWDDLVKEGVEVIFPNPKTSGNARYTYLAAYAYALEANGGDQAKAQEFVKQ
ECCCCCCCHHHHHHHHCCCEEEECCCCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHH
LFANVPVFDQGGRAATVTFAEKGIGDVLITFEAETGGIAAQYADQGLERVTPSLSLYSEF
HHHCCCEECCCCCEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHCCC
PVAIVTENAERNGSAEVSKSYLDFLYAPEGQKILAEHFYRVHDETAQAAFAANFPEVKLV
CEEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
DVDETFGGWEKIKAEHFAEGGILDTVFVNQ
ECCCCCCCHHHHHHHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: SO42- [Periplasm]; H2O; ATP; S2O32- [Periplasm] [C]

Specific reaction: SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2188959; 9205837; 9278503; 9298646 [H]