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The map label for this gene is lutR [H]

Identifier: 77465671

GI number: 77465671

Start: 771798

End: 772547

Strand: Reverse

Name: lutR [H]

Synonym: RSP_3664

Alternate gene names: 77465671

Gene position: 772547-771798 (Counterclockwise)

Preceding gene: 77465673

Following gene: 77465670

Centisome position: 81.92

GC content: 67.73

Gene sequence:

>750_bases
ATGCCAGCTCATCAGTTGTCTGATGACCGTGCAATACAGGGGACCTTCATGAGAAAACGCCGTGACAGCCTGGCTTCCGT
CGTCGCCCGGGATCTGGCCGGGCGCATCGAGCGTGGCGACTACGCCGTGGGCGACAAGATCCCCATCGAAGCGCAGCTGT
GCGAGATGTACAACGTGAGCCGGACCGTGGTGCGCGAGGCCATCGCGACGCTGCGCTCCGAGGGGCTGGTGACATCGCGC
CACGGCGTCGGCGTGTTCGTCGAGGAGCGCGGCAATCCGGCGCTGTCGATCACCCGGGAGGCGGCGGGCGAGCTCTCGGA
GGTGCTCGAACTGCTCGAGCTCCGGCTCGGGGTCGAGGTGGAAGCCGCCGGCCTTGCGGCCCGGAGGGCCACCGCGGAAG
ACCTGCGCCAGATCCGGGAGGTGCTCGAGAAGACGGACGATCTCGAGGCCTTCGCCGAGCACCGGCGCGAGCTGGACTAT
GCCTTCCATCTGGCGATCGCGCGGGCGAGCGGGAACAGCTGGATGCCGAAGTTCATGGATTTCATCGGCCCCGTCATCAT
TCCGCGCAGCCAGCTTCTGCACGACCCGCAATCGGGCATCACCGGCGACTACCTGCGCCAGATGGTCGAGGAGCACCGGC
AGATCGTCGAGGCCATCGCCGCGGGCGAAGCGGCCGCGGCGCAGGAGGCGATGCGGATGCATCTCTCCACCTCGCTGGAG
CGCTACCGTCGCCGGCTGACCGACGCATAA

Upstream 100 bases:

>100_bases
ACCGGCAACGAAGGACATGCTCCTCATGCGGCCCAACGCGCCCGTCGCTCGGGCCGACCGCTCCGCGGAGGCAGTTGCCG
CCCGTCGTTCCGCCCTGTAC

Downstream 100 bases:

>100_bases
CTCATAATTCAACAGTCATCATTTGACAGACATCATACAATTAGCTATCGAAGGTTCAGGGAGGTAGCCGCGTCGAGTCT
GCGCGCAGATCCCGGACAGA

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MPAHQLSDDRAIQGTFMRKRRDSLASVVARDLAGRIERGDYAVGDKIPIEAQLCEMYNVSRTVVREAIATLRSEGLVTSR
HGVGVFVEERGNPALSITREAAGELSEVLELLELRLGVEVEAAGLAARRATAEDLRQIREVLEKTDDLEAFAEHRRELDY
AFHLAIARASGNSWMPKFMDFIGPVIIPRSQLLHDPQSGITGDYLRQMVEEHRQIVEAIAAGEAAAAQEAMRMHLSTSLE
RYRRRLTDA

Sequences:

>Translated_249_residues
MPAHQLSDDRAIQGTFMRKRRDSLASVVARDLAGRIERGDYAVGDKIPIEAQLCEMYNVSRTVVREAIATLRSEGLVTSR
HGVGVFVEERGNPALSITREAAGELSEVLELLELRLGVEVEAAGLAARRATAEDLRQIREVLEKTDDLEAFAEHRRELDY
AFHLAIARASGNSWMPKFMDFIGPVIIPRSQLLHDPQSGITGDYLRQMVEEHRQIVEAIAAGEAAAAQEAMRMHLSTSLE
RYRRRLTDA
>Mature_248_residues
PAHQLSDDRAIQGTFMRKRRDSLASVVARDLAGRIERGDYAVGDKIPIEAQLCEMYNVSRTVVREAIATLRSEGLVTSRH
GVGVFVEERGNPALSITREAAGELSEVLELLELRLGVEVEAAGLAARRATAEDLRQIREVLEKTDDLEAFAEHRRELDYA
FHLAIARASGNSWMPKFMDFIGPVIIPRSQLLHDPQSGITGDYLRQMVEEHRQIVEAIAAGEAAAAQEAMRMHLSTSLER
YRRRLTDA

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082208, Length=223, Percent_Identity=32.2869955156951, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1790032, Length=232, Percent_Identity=31.8965517241379, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1790780, Length=226, Percent_Identity=31.858407079646, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI48994961, Length=220, Percent_Identity=30, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1786303, Length=240, Percent_Identity=30.4166666666667, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789353, Length=236, Percent_Identity=28.8135593220339, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI48994955, Length=229, Percent_Identity=27.5109170305677, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27789; Mature: 27658

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAHQLSDDRAIQGTFMRKRRDSLASVVARDLAGRIERGDYAVGDKIPIEAQLCEMYNVS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
RTVVREAIATLRSEGLVTSRHGVGVFVEERGNPALSITREAAGELSEVLELLELRLGVEV
HHHHHHHHHHHHHCCCEEECCCCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCC
EAAGLAARRATAEDLRQIREVLEKTDDLEAFAEHRRELDYAFHLAIARASGNSWMPKFMD
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
FIGPVIIPRSQLLHDPQSGITGDYLRQMVEEHRQIVEAIAAGEAAAAQEAMRMHLSTSLE
HHCHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
RYRRRLTDA
HHHHHHCCC
>Mature Secondary Structure 
PAHQLSDDRAIQGTFMRKRRDSLASVVARDLAGRIERGDYAVGDKIPIEAQLCEMYNVS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
RTVVREAIATLRSEGLVTSRHGVGVFVEERGNPALSITREAAGELSEVLELLELRLGVEV
HHHHHHHHHHHHHCCCEEECCCCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCC
EAAGLAARRATAEDLRQIREVLEKTDDLEAFAEHRRELDYAFHLAIARASGNSWMPKFMD
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
FIGPVIIPRSQLLHDPQSGITGDYLRQMVEEHRQIVEAIAAGEAAAAQEAMRMHLSTSLE
HHCHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
RYRRRLTDA
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]