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The map label for this gene is hisS

Identifier: 77465550

GI number: 77465550

Start: 641093

End: 642577

Strand: Reverse

Name: hisS

Synonym: RSP_3551

Alternate gene names: 77465550

Gene position: 642577-641093 (Counterclockwise)

Preceding gene: 77465552

Following gene: 77465549

Centisome position: 68.14

GC content: 69.97

Gene sequence:

>1485_bases
ATGGCCAAGGACAAGGCTTCCCGCCGCCCGCGCGCGGAAACGCCCAAGGGCTTCCGCGACTATTTCGGTGCGGATGTGAC
CGAGCGCAAGGCGATGCTCGACGCCGTGGCCGAGGTCTACCATCGCTACGGCTTCGATCCGCTGGAAACCTCGGCCGTCG
AGACCGTCGAGGCGCTGGGCAAGTTCCTGCCCGATGTGGATCGCCCGAACGAGGGCGTCTTCGGCTGGCAGGACGAGGAT
GGCGACTGGCTCGCGCTGCGCTACGATCTGACGGCGCCGCTGGCGCGGGTCGCGGCGCAGTTCCGCAACGACCTGCCGAG
CCCCTACCGGCGCTATGCGATGGGCCCGGTCTGGCGCAACGAGAAGCCGGGGCCGGGGCGGTTCCGCCAGTTCTATCAAT
GCGACGCGGATACGGTGGGGTCGGCGAGCGTCGCGGCCGATGCCGAGATCTGCGCCATGCTGTCGGACGCGCTCGAAGTC
GTGGGCATTCCCCGCGGCGACTATATCGTGCGGGTGAACAACCGGAAGGTCCTGAACGGGGTGATGGAGGTGGCGGGCGT
GCTCGACCCGTCCGACCCGGCGAAGTTCGAGGCCGAGCGCGGCATCGTGCTCCGCGCCATCGACAAGATCGACCGGCTGG
GTGAGACGGGCGTGCGCGCACTGCTGGGCTCGGGCCGCAAGGACGAGAGCGGCGATTTCACCAAGGGCGCGGGGCTCTCG
GACGAGCAGGCCGAGGTGGTCATGGGCTTCATGGCCGCCAGGCGCGACACGGGCGCGGCGACGGCTGCGCGGCTGCGCGA
GCTGGTGGGGGCCTCGACCCTCGGGTGCGAGGGCGTGCAGGAGCTCGAAACCATCGCCGAACTGCTTGACGTGCAGGGCT
ACGGGCCCGACCGGATCGTGGTGGATCCGTCGGTGGTGCGGGGCCTCGGCTATTATACCGGCCCGGTGTTCGAGGCCGAG
CTTACCTTCGAGATCCTCGACGAGAAGGGCCGCAAGCGGCAGTTCGGCTCGGTCGCGGGCGGCGGGCGCTATGACGATCT
GGTCAAGCGCTTCACCGGCCAGTCGGTGCCGGCGACCGGCGTCTCGATCGGGGTCGACCGGCTGCTGGCGGCACTGCGCG
CCAAGGGTCGTGCGGGGGCCGAGGCGCAGGGGCCGGTGGTGGTGACCGTCATGGACCGCGACCGGATGGGCGACTACATG
GCCATGGTGGGCGAGTTGCGCCGCGCGGGCCTCCGGGCCGAACTCTATCTCGGCAATCCGAAGAACTTCGGCAACCAGCT
GAAATATGCGGATGCGCGGAAGTCTCCGGTGGCGGTCATCCAGGGGTCGGACGAGGCCGCGCGCGGGGTCGTGGTGCTGA
AGGATCTGGTGCTCGGCGCGCAGATCGCGGCCGGCGCGAGCCTCGAGGAATGGAAGAGCCGGCCAGCGCAGGTCGAGGTG
CCGCGGGCGGATCTCGTCCGGGCGGTGCAGGACATGCTCTCGTGA

Upstream 100 bases:

>100_bases
TCCGCTCGTCCATCGTCTGCTCCTCGTGTTTCGCCCGGCGCTTGTCCGCGGCCCGCCCATCCGATACACCGCGCGCGACG
AACAGCGAAGGGTGCGATCC

Downstream 100 bases:

>100_bases
CCAAGGCCGCGGTCCGCGCCGAGGCCGAGGCGCTGTTCGCGGCCTTTCAGGCGGCGGGGGCGCTGCCGGTCGAGGCGGAC
ATCCTGCTGCCCGCGGGCAC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MAKDKASRRPRAETPKGFRDYFGADVTERKAMLDAVAEVYHRYGFDPLETSAVETVEALGKFLPDVDRPNEGVFGWQDED
GDWLALRYDLTAPLARVAAQFRNDLPSPYRRYAMGPVWRNEKPGPGRFRQFYQCDADTVGSASVAADAEICAMLSDALEV
VGIPRGDYIVRVNNRKVLNGVMEVAGVLDPSDPAKFEAERGIVLRAIDKIDRLGETGVRALLGSGRKDESGDFTKGAGLS
DEQAEVVMGFMAARRDTGAATAARLRELVGASTLGCEGVQELETIAELLDVQGYGPDRIVVDPSVVRGLGYYTGPVFEAE
LTFEILDEKGRKRQFGSVAGGGRYDDLVKRFTGQSVPATGVSIGVDRLLAALRAKGRAGAEAQGPVVVTVMDRDRMGDYM
AMVGELRRAGLRAELYLGNPKNFGNQLKYADARKSPVAVIQGSDEAARGVVVLKDLVLGAQIAAGASLEEWKSRPAQVEV
PRADLVRAVQDMLS

Sequences:

>Translated_494_residues
MAKDKASRRPRAETPKGFRDYFGADVTERKAMLDAVAEVYHRYGFDPLETSAVETVEALGKFLPDVDRPNEGVFGWQDED
GDWLALRYDLTAPLARVAAQFRNDLPSPYRRYAMGPVWRNEKPGPGRFRQFYQCDADTVGSASVAADAEICAMLSDALEV
VGIPRGDYIVRVNNRKVLNGVMEVAGVLDPSDPAKFEAERGIVLRAIDKIDRLGETGVRALLGSGRKDESGDFTKGAGLS
DEQAEVVMGFMAARRDTGAATAARLRELVGASTLGCEGVQELETIAELLDVQGYGPDRIVVDPSVVRGLGYYTGPVFEAE
LTFEILDEKGRKRQFGSVAGGGRYDDLVKRFTGQSVPATGVSIGVDRLLAALRAKGRAGAEAQGPVVVTVMDRDRMGDYM
AMVGELRRAGLRAELYLGNPKNFGNQLKYADARKSPVAVIQGSDEAARGVVVLKDLVLGAQIAAGASLEEWKSRPAQVEV
PRADLVRAVQDMLS
>Mature_493_residues
AKDKASRRPRAETPKGFRDYFGADVTERKAMLDAVAEVYHRYGFDPLETSAVETVEALGKFLPDVDRPNEGVFGWQDEDG
DWLALRYDLTAPLARVAAQFRNDLPSPYRRYAMGPVWRNEKPGPGRFRQFYQCDADTVGSASVAADAEICAMLSDALEVV
GIPRGDYIVRVNNRKVLNGVMEVAGVLDPSDPAKFEAERGIVLRAIDKIDRLGETGVRALLGSGRKDESGDFTKGAGLSD
EQAEVVMGFMAARRDTGAATAARLRELVGASTLGCEGVQELETIAELLDVQGYGPDRIVVDPSVVRGLGYYTGPVFEAEL
TFEILDEKGRKRQFGSVAGGGRYDDLVKRFTGQSVPATGVSIGVDRLLAALRAKGRAGAEAQGPVVVTVMDRDRMGDYMA
MVGELRRAGLRAELYLGNPKNFGNQLKYADARKSPVAVIQGSDEAARGVVVLKDLVLGAQIAAGASLEEWKSRPAQVEVP
RADLVRAVQDMLS

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI15029520, Length=493, Percent_Identity=30.8316430020284, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI6996014, Length=500, Percent_Identity=30.8, Blast_Score=192, Evalue=5e-49,
Organism=Escherichia coli, GI1788861, Length=189, Percent_Identity=30.6878306878307, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71993693, Length=468, Percent_Identity=31.4102564102564, Blast_Score=186, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI71993686, Length=468, Percent_Identity=31.1965811965812, Blast_Score=186, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6325290, Length=498, Percent_Identity=27.3092369477912, Blast_Score=160, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24643061, Length=476, Percent_Identity=31.0924369747899, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24643059, Length=495, Percent_Identity=29.8989898989899, Blast_Score=182, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_RHOS4 (Q3IWD2)

Other databases:

- EMBL:   CP000144
- RefSeq:   YP_355053.1
- ProteinModelPortal:   Q3IWD2
- SMR:   Q3IWD2
- STRING:   Q3IWD2
- GeneID:   3721965
- GenomeReviews:   CP000144_GR
- KEGG:   rsp:RSP_3551
- NMPDR:   fig|272943.3.peg.3297
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   ALDIARC
- PhylomeDB:   Q3IWD2
- ProtClustDB:   PRK00037
- BioCyc:   RSPH272943:RSP_3551-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 53497; Mature: 53366

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDKASRRPRAETPKGFRDYFGADVTERKAMLDAVAEVYHRYGFDPLETSAVETVEALG
CCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KFLPDVDRPNEGVFGWQDEDGDWLALRYDLTAPLARVAAQFRNDLPSPYRRYAMGPVWRN
HHCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCC
EKPGPGRFRQFYQCDADTVGSASVAADAEICAMLSDALEVVGIPRGDYIVRVNNRKVLNG
CCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHH
VMEVAGVLDPSDPAKFEAERGIVLRAIDKIDRLGETGVRALLGSGRKDESGDFTKGAGLS
HHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
DEQAEVVMGFMAARRDTGAATAARLRELVGASTLGCEGVQELETIAELLDVQGYGPDRIV
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEE
VDPSVVRGLGYYTGPVFEAELTFEILDEKGRKRQFGSVAGGGRYDDLVKRFTGQSVPATG
ECHHHHHHCCHHCCCEEEHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHCCCCCCCCC
VSIGVDRLLAALRAKGRAGAEAQGPVVVTVMDRDRMGDYMAMVGELRRAGLRAELYLGNP
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEECCC
KNFGNQLKYADARKSPVAVIQGSDEAARGVVVLKDLVLGAQIAAGASLEEWKSRPAQVEV
CCCCCCCEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEC
PRADLVRAVQDMLS
CHHHHHHHHHHHHC
>Mature Secondary Structure 
AKDKASRRPRAETPKGFRDYFGADVTERKAMLDAVAEVYHRYGFDPLETSAVETVEALG
CCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KFLPDVDRPNEGVFGWQDEDGDWLALRYDLTAPLARVAAQFRNDLPSPYRRYAMGPVWRN
HHCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCC
EKPGPGRFRQFYQCDADTVGSASVAADAEICAMLSDALEVVGIPRGDYIVRVNNRKVLNG
CCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHH
VMEVAGVLDPSDPAKFEAERGIVLRAIDKIDRLGETGVRALLGSGRKDESGDFTKGAGLS
HHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
DEQAEVVMGFMAARRDTGAATAARLRELVGASTLGCEGVQELETIAELLDVQGYGPDRIV
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEE
VDPSVVRGLGYYTGPVFEAELTFEILDEKGRKRQFGSVAGGGRYDDLVKRFTGQSVPATG
ECHHHHHHCCHHCCCEEEHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHCCCCCCCCC
VSIGVDRLLAALRAKGRAGAEAQGPVVVTVMDRDRMGDYMAMVGELRRAGLRAELYLGNP
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEECCC
KNFGNQLKYADARKSPVAVIQGSDEAARGVVVLKDLVLGAQIAAGASLEEWKSRPAQVEV
CCCCCCCEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEC
PRADLVRAVQDMLS
CHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA