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The map label for this gene is yafV [C]

Identifier: 77465463

GI number: 77465463

Start: 530296

End: 531222

Strand: Direct

Name: yafV [C]

Synonym: RSP_3461

Alternate gene names: 77465463

Gene position: 530296-531222 (Clockwise)

Preceding gene: 77465462

Following gene: 77465464

Centisome position: 56.23

GC content: 65.91

Gene sequence:

>927_bases
ATGCGCAAGATGATCGTTGGCGGGGCCCAGATGGGGCCGATCCAGCAGGCCGACAGCCGCGAGACGGTGGTGGCCCGGAT
GATGGAGCTGATGCGCAAGGCCCATGCCGAGGGCGTGAAGATGCTCGTCTACCCCGAGCTCGCCCTCACCACCTTCTTCC
CACGCTGGTATGCCGAGGACCGCGCCGAGATGGACCGCTGGTTCGAGAGCGAGATGCCGAACGGGGCCACGCAGCCTCTG
TTCGATCTGGCGCGCGAGATGGGCATGGCCTTCACCTTCGGCTTTGCCGAGTTGACGCCCGATGGGCACCATTTCAACAC
CTCGATCCTGACGGATGCGCAGGCCAACATCGTGGGCACCTACCGCAAGGTCCACCTGCCCGGCCATGTGGAGTTCGACC
CCGAGCGCACCCACCAGCATCTCGAGAAACGCTATTTCGAGCCGGGCGATCTCGGCTTCAACGTCTGGCGCAACGAAGGC
GTGGTCATGGGCATGGCGATCTGCAACGATCGCCGCTGGCCGGAAACCTACCGCGAGCTGGGGTTGCAGGGCGTGGAACT
CGTCACCATCGGCTACAACACCCCGTCCGTGAACAGCCAGAAGGGCGCCGAGGGGCTGCGCCAGCGGCTGTTCCACTCGG
ATCTGTCGATGCAGGCCGGGGCCTACCAGAACTCGACCTACGTCGTGGGCGTGGCCAAGGCCGGCACCGAGGACGGCCAT
CACCTGATGGGCGGCTCGATCATCGTCGATCCCGACGGCGAGGTGATCGCCCGCGCCGAGACCGAGGGCGACGAGCTGAT
CTTCGCCGAATGCGACTTCGACCGCTGCACCTTCGGCAAGGCCACCGTCTTCGACTTCGCCCGCCACCGGCGGATCGAGC
ATTATCAGCGCATCGGCCGGCAGACCGGCGTGATCGTCCCGGTCTGA

Upstream 100 bases:

>100_bases
CTCGTGCTGCTCTGGCCGCTGGTGCGGCTCGTCAGCCGCTACCAGCGCCGCCTCGCGGCCTGAGCACCCCTTTCACCACT
TACCTTCAGGGAAGAGACAG

Downstream 100 bases:

>100_bases
GGAGAGGGCAGCATGGAGTTCGATACGGTCATCCACGGCGGCACCATCGTCACGCCGACCGAAAGCTGGCAGGGCGATCT
GGGCCTCGTGGGGGGCCGGA

Product: N-carbamyl-D-amino acid amidohydrolase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MRKMIVGGAQMGPIQQADSRETVVARMMELMRKAHAEGVKMLVYPELALTTFFPRWYAEDRAEMDRWFESEMPNGATQPL
FDLAREMGMAFTFGFAELTPDGHHFNTSILTDAQANIVGTYRKVHLPGHVEFDPERTHQHLEKRYFEPGDLGFNVWRNEG
VVMGMAICNDRRWPETYRELGLQGVELVTIGYNTPSVNSQKGAEGLRQRLFHSDLSMQAGAYQNSTYVVGVAKAGTEDGH
HLMGGSIIVDPDGEVIARAETEGDELIFAECDFDRCTFGKATVFDFARHRRIEHYQRIGRQTGVIVPV

Sequences:

>Translated_308_residues
MRKMIVGGAQMGPIQQADSRETVVARMMELMRKAHAEGVKMLVYPELALTTFFPRWYAEDRAEMDRWFESEMPNGATQPL
FDLAREMGMAFTFGFAELTPDGHHFNTSILTDAQANIVGTYRKVHLPGHVEFDPERTHQHLEKRYFEPGDLGFNVWRNEG
VVMGMAICNDRRWPETYRELGLQGVELVTIGYNTPSVNSQKGAEGLRQRLFHSDLSMQAGAYQNSTYVVGVAKAGTEDGH
HLMGGSIIVDPDGEVIARAETEGDELIFAECDFDRCTFGKATVFDFARHRRIEHYQRIGRQTGVIVPV
>Mature_308_residues
MRKMIVGGAQMGPIQQADSRETVVARMMELMRKAHAEGVKMLVYPELALTTFFPRWYAEDRAEMDRWFESEMPNGATQPL
FDLAREMGMAFTFGFAELTPDGHHFNTSILTDAQANIVGTYRKVHLPGHVEFDPERTHQHLEKRYFEPGDLGFNVWRNEG
VVMGMAICNDRRWPETYRELGLQGVELVTIGYNTPSVNSQKGAEGLRQRLFHSDLSMQAGAYQNSTYVVGVAKAGTEDGH
HLMGGSIIVDPDGEVIARAETEGDELIFAECDFDRCTFGKATVFDFARHRRIEHYQRIGRQTGVIVPV

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=268, Percent_Identity=23.5074626865672, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323383, Length=268, Percent_Identity=27.2388059701493, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 34794; Mature: 34794

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKMIVGGAQMGPIQQADSRETVVARMMELMRKAHAEGVKMLVYPELALTTFFPRWYAED
CCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHH
RAEMDRWFESEMPNGATQPLFDLAREMGMAFTFGFAELTPDGHHFNTSILTDAQANIVGT
HHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEECHHHCCCCCCEECCEEECCCCCCEEEE
YRKVHLPGHVEFDPERTHQHLEKRYFEPGDLGFNVWRNEGVVMGMAICNDRRWPETYREL
EEEEECCCCEECCHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEEEECCCCCCHHHHHHH
GLQGVELVTIGYNTPSVNSQKGAEGLRQRLFHSDLSMQAGAYQNSTYVVGVAKAGTEDGH
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCC
HLMGGSIIVDPDGEVIARAETEGDELIFAECDFDRCTFGKATVFDFARHRRIEHYQRIGR
EEECCEEEECCCCCEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QTGVIVPV
HCCCEEEC
>Mature Secondary Structure
MRKMIVGGAQMGPIQQADSRETVVARMMELMRKAHAEGVKMLVYPELALTTFFPRWYAED
CCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHH
RAEMDRWFESEMPNGATQPLFDLAREMGMAFTFGFAELTPDGHHFNTSILTDAQANIVGT
HHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEECHHHCCCCCCEECCEEECCCCCCEEEE
YRKVHLPGHVEFDPERTHQHLEKRYFEPGDLGFNVWRNEGVVMGMAICNDRRWPETYREL
EEEEECCCCEECCHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEEEECCCCCCHHHHHHH
GLQGVELVTIGYNTPSVNSQKGAEGLRQRLFHSDLSMQAGAYQNSTYVVGVAKAGTEDGH
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCC
HLMGGSIIVDPDGEVIARAETEGDELIFAECDFDRCTFGKATVFDFARHRRIEHYQRIGR
EEECCEEEECCCCCEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QTGVIVPV
HCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]