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The map label for this gene is ygeZ [C]

Identifier: 77465464

GI number: 77465464

Start: 531235

End: 532692

Strand: Direct

Name: ygeZ [C]

Synonym: RSP_3462

Alternate gene names: 77465464

Gene position: 531235-532692 (Clockwise)

Preceding gene: 77465463

Following gene: 77465465

Centisome position: 56.33

GC content: 69.96

Gene sequence:

>1458_bases
ATGGAGTTCGATACGGTCATCCACGGCGGCACCATCGTCACGCCGACCGAAAGCTGGCAGGGCGATCTGGGCCTCGTGGG
GGGCCGGATCGCGGGTCTGGCCGAGCGGCTGCCCGGCGGCGCGCGCCGGATCGACGCCACCGGGCGGCTCGTCCTGCCCG
GCGGCATCGAGGCGCACGCCCATATCGCGCAGGAAAGCTCCTCGGGGCTGATGAGCGCGGACGACTATTACACGGGCTCG
GTCTCGGCGGCCTTCGGCGGCAACTCGAGCTTCATCCCCTTCGCGGCCCAGCACCGCGGGCAGTCGGTGGATGCGGTGAT
CGAGACCTACGACAGCCGGGCGGCGCCGAACTCGGTGCTCGACTATTCCTACCATCTCATCATCTCGGACCCGACCGAGA
CCGTCCTGACCGAAGAGCTGCCGCGCGCCTTCGCCCGCGGCATCACCTCGTTCAAGGTCTTCATGACCTACGACCTGATG
AACCTCGGCGACCGCGGGATGCTCGACATCCTGACCGTCGCCCGCCGTCACGGCGCCCTCACCATGGTCCATGCCGAGAA
CAACGACATGGTGAAATGGATGAACGCGCGCCTTGCCGCGGCAGGGCTCACGGCGCCGAAATATCACGCGATCTCGCGCC
CGGCGCTGGCCGAGGCCGAGGCGATCAACCGCGCGATCTCGCTCGCGCGGCTCGTGGGGGCGGGGCTCTTCATCGTCCAT
GTCTCGACGCCCGAGGGGGCGGATCTCGTGGCCCGCGCGCAGGCCTCGGGTCTGCCGATCCATGCGGAGACCTGCCCGCA
GTATCTGGCCTTCACCCGCGACGACCTCGACCGGCCGGGGATGGAGGGGGCCAAATACATCTGCTCGCCCCCCCTGCGCG
ATGCGGCGACGCAGGCCGCGCTCTGGAACCATGCCCGGCGCGGCACCTTCGAGAGCGTGTCCTCGGACCATGCCCCCTAC
CGGTTCGACGCGAGCGGCAAGTTCGCGAACGGCGCAGAGCCCGCCTATCCCGCCATCGCGAACGGCCTTCCGGGCATCGC
CATGCGCCTGCCCTATCTCTTCTCCGAAGGAGTCGCGGCCGGGCGGATCAGCCTCCAGCAGTTCGCGGCCCTCTCTTCCT
CGAACGCCGCCCGCCTCTTCGGGATGGAGCGCAAGGGCGCGCTGCTGCCGGGCTATGACGCCGACATCGCGATCTGGAAC
CCCGAGGAAACGCGCGAGGTCTCGCTCGCCGATCAGCACGATGCCATGGACTACACACCCTTCGAGGGGATGCGCCTCAC
CGGTTGGCCCGAGCATGTGCTGAGCCGCGGCGAGACGGTGGTCGAGGCGGGCGAGCTGAAGGCCGCCCGCGGGCGCGGCC
GCTTCGTCGCGCGTGCCCCCTACCGCCCCGATCCCAACGCGCCGGTCGAGCCCGAGCTCACCCCCGCGCTTAACTTCGGC
GCGGAGATCCGGCCGTGA

Upstream 100 bases:

>100_bases
GGCCACCGTCTTCGACTTCGCCCGCCACCGGCGGATCGAGCATTATCAGCGCATCGGCCGGCAGACCGGCGTGATCGTCC
CGGTCTGAGGAGAGGGCAGC

Downstream 100 bases:

>100_bases
AGCGCATCCTCGTCATCAACCCCAACTCCTCGACCGCCGTCACCGAAGGGCTGCGCGAGGCGCTCGCCCCCTTCGAGGGG
TCCGTCCGTTTCGACTGCAT

Product: dihydropyrimidinase

Products: 3-ureidopropanoate

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MEFDTVIHGGTIVTPTESWQGDLGLVGGRIAGLAERLPGGARRIDATGRLVLPGGIEAHAHIAQESSSGLMSADDYYTGS
VSAAFGGNSSFIPFAAQHRGQSVDAVIETYDSRAAPNSVLDYSYHLIISDPTETVLTEELPRAFARGITSFKVFMTYDLM
NLGDRGMLDILTVARRHGALTMVHAENNDMVKWMNARLAAAGLTAPKYHAISRPALAEAEAINRAISLARLVGAGLFIVH
VSTPEGADLVARAQASGLPIHAETCPQYLAFTRDDLDRPGMEGAKYICSPPLRDAATQAALWNHARRGTFESVSSDHAPY
RFDASGKFANGAEPAYPAIANGLPGIAMRLPYLFSEGVAAGRISLQQFAALSSSNAARLFGMERKGALLPGYDADIAIWN
PEETREVSLADQHDAMDYTPFEGMRLTGWPEHVLSRGETVVEAGELKAARGRGRFVARAPYRPDPNAPVEPELTPALNFG
AEIRP

Sequences:

>Translated_485_residues
MEFDTVIHGGTIVTPTESWQGDLGLVGGRIAGLAERLPGGARRIDATGRLVLPGGIEAHAHIAQESSSGLMSADDYYTGS
VSAAFGGNSSFIPFAAQHRGQSVDAVIETYDSRAAPNSVLDYSYHLIISDPTETVLTEELPRAFARGITSFKVFMTYDLM
NLGDRGMLDILTVARRHGALTMVHAENNDMVKWMNARLAAAGLTAPKYHAISRPALAEAEAINRAISLARLVGAGLFIVH
VSTPEGADLVARAQASGLPIHAETCPQYLAFTRDDLDRPGMEGAKYICSPPLRDAATQAALWNHARRGTFESVSSDHAPY
RFDASGKFANGAEPAYPAIANGLPGIAMRLPYLFSEGVAAGRISLQQFAALSSSNAARLFGMERKGALLPGYDADIAIWN
PEETREVSLADQHDAMDYTPFEGMRLTGWPEHVLSRGETVVEAGELKAARGRGRFVARAPYRPDPNAPVEPELTPALNFG
AEIRP
>Mature_485_residues
MEFDTVIHGGTIVTPTESWQGDLGLVGGRIAGLAERLPGGARRIDATGRLVLPGGIEAHAHIAQESSSGLMSADDYYTGS
VSAAFGGNSSFIPFAAQHRGQSVDAVIETYDSRAAPNSVLDYSYHLIISDPTETVLTEELPRAFARGITSFKVFMTYDLM
NLGDRGMLDILTVARRHGALTMVHAENNDMVKWMNARLAAAGLTAPKYHAISRPALAEAEAINRAISLARLVGAGLFIVH
VSTPEGADLVARAQASGLPIHAETCPQYLAFTRDDLDRPGMEGAKYICSPPLRDAATQAALWNHARRGTFESVSSDHAPY
RFDASGKFANGAEPAYPAIANGLPGIAMRLPYLFSEGVAAGRISLQQFAALSSSNAARLFGMERKGALLPGYDADIAIWN
PEETREVSLADQHDAMDYTPFEGMRLTGWPEHVLSRGETVVEAGELKAARGRGRFVARAPYRPDPNAPVEPELTPALNFG
AEIRP

Specific function: Catalyzes the stereospecific hydrolysis of the cyclic amide bond of D-hydantoin. Has no activity on dihydropyrimidines [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503051, Length=462, Percent_Identity=32.2510822510823, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI4503375, Length=466, Percent_Identity=33.2618025751073, Blast_Score=247, Evalue=2e-65,
Organism=Homo sapiens, GI62422571, Length=462, Percent_Identity=32.2510822510823, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI4503377, Length=465, Percent_Identity=32.258064516129, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI4503379, Length=462, Percent_Identity=30.952380952381, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI19923821, Length=467, Percent_Identity=32.9764453961456, Blast_Score=234, Evalue=2e-61,
Organism=Homo sapiens, GI190194363, Length=465, Percent_Identity=32.4731182795699, Blast_Score=231, Evalue=8e-61,
Organism=Homo sapiens, GI18105007, Length=433, Percent_Identity=25.8660508083141, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI87082175, Length=471, Percent_Identity=38.0042462845011, Blast_Score=291, Evalue=4e-80,
Organism=Escherichia coli, GI1786722, Length=478, Percent_Identity=27.4058577405858, Blast_Score=181, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI17539558, Length=462, Percent_Identity=32.6839826839827, Blast_Score=221, Evalue=8e-58,
Organism=Caenorhabditis elegans, GI71989490, Length=490, Percent_Identity=31.8367346938775, Blast_Score=201, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI86575075, Length=463, Percent_Identity=24.4060475161987, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI193204318, Length=414, Percent_Identity=24.3961352657005, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6322218, Length=406, Percent_Identity=24.1379310344828, Blast_Score=104, Evalue=3e-23,
Organism=Drosophila melanogaster, GI17137462, Length=464, Percent_Identity=34.051724137931, Blast_Score=245, Evalue=5e-65,
Organism=Drosophila melanogaster, GI221377917, Length=476, Percent_Identity=34.2436974789916, Blast_Score=241, Evalue=6e-64,
Organism=Drosophila melanogaster, GI24644287, Length=308, Percent_Identity=33.7662337662338, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI24644289, Length=231, Percent_Identity=35.0649350649351, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24642586, Length=421, Percent_Identity=22.5653206650831, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI18859883, Length=245, Percent_Identity=26.9387755102041, Blast_Score=79, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011778
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.5.2.2

Molecular weight: Translated: 52012; Mature: 52012

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFDTVIHGGTIVTPTESWQGDLGLVGGRIAGLAERLPGGARRIDATGRLVLPGGIEAHA
CCCCEEEECCEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCEEECCCCCHHH
HIAQESSSGLMSADDYYTGSVSAAFGGNSSFIPFAAQHRGQSVDAVIETYDSRAAPNSVL
HHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCHHHHHHHHHCCCCCCCEE
DYSYHLIISDPTETVLTEELPRAFARGITSFKVFMTYDLMNLGDRGMLDILTVARRHGAL
CEEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEEEEHHCCCCCCHHHHHHHHHHCCCE
TMVHAENNDMVKWMNARLAAAGLTAPKYHAISRPALAEAEAINRAISLARLVGAGLFIVH
EEEEECCCCEEHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
VSTPEGADLVARAQASGLPIHAETCPQYLAFTRDDLDRPGMEGAKYICSPPLRDAATQAA
ECCCCCCCHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCEECCCCHHHHHHHHH
LWNHARRGTFESVSSDHAPYRFDASGKFANGAEPAYPAIANGLPGIAMRLPYLFSEGVAA
HHHHHHCCCHHHHCCCCCCEEECCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCC
GRISLQQFAALSSSNAARLFGMERKGALLPGYDADIAIWNPEETREVSLADQHDAMDYTP
CHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCEEEECCCCCCEEECCCCCCCCCCCC
FEGMRLTGWPEHVLSRGETVVEAGELKAARGRGRFVARAPYRPDPNAPVEPELTPALNFG
CCCEEECCCHHHHHHCCCEEEECCCHHCCCCCCCEEEECCCCCCCCCCCCCCCCHHHCCC
AEIRP
CCCCC
>Mature Secondary Structure
MEFDTVIHGGTIVTPTESWQGDLGLVGGRIAGLAERLPGGARRIDATGRLVLPGGIEAHA
CCCCEEEECCEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCEEECCCCCHHH
HIAQESSSGLMSADDYYTGSVSAAFGGNSSFIPFAAQHRGQSVDAVIETYDSRAAPNSVL
HHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCHHHHHHHHHCCCCCCCEE
DYSYHLIISDPTETVLTEELPRAFARGITSFKVFMTYDLMNLGDRGMLDILTVARRHGAL
CEEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEEEEHHCCCCCCHHHHHHHHHHCCCE
TMVHAENNDMVKWMNARLAAAGLTAPKYHAISRPALAEAEAINRAISLARLVGAGLFIVH
EEEEECCCCEEHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
VSTPEGADLVARAQASGLPIHAETCPQYLAFTRDDLDRPGMEGAKYICSPPLRDAATQAA
ECCCCCCCHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCEECCCCHHHHHHHHH
LWNHARRGTFESVSSDHAPYRFDASGKFANGAEPAYPAIANGLPGIAMRLPYLFSEGVAA
HHHHHHCCCHHHHCCCCCCEEECCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCC
GRISLQQFAALSSSNAARLFGMERKGALLPGYDADIAIWNPEETREVSLADQHDAMDYTP
CHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCEEEECCCCCCEEECCCCCCCCCCCC
FEGMRLTGWPEHVLSRGETVVEAGELKAARGRGRFVARAPYRPDPNAPVEPELTPALNFG
CCCEEECCCHHHHHHCCCEEEECCCHHCCCCCCCEEEECCCCCCCCCCCCCCCCHHHCCC
AEIRP
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5,6-dihydrouracil; H2O

Specific reaction: 5,6-dihydrouracil + H2O = 3-ureidopropanoate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7765480 [H]