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The map label for this gene is gntR [H]

Identifier: 77465407

GI number: 77465407

Start: 471417

End: 472136

Strand: Direct

Name: gntR [H]

Synonym: RSP_3405

Alternate gene names: 77465407

Gene position: 471417-472136 (Clockwise)

Preceding gene: 77465406

Following gene: 77465414

Centisome position: 49.99

GC content: 67.78

Gene sequence:

>720_bases
ATGGCCCATCTCAAACAGTCGACCCGAATGCCGCTGGCCGACAAGGTCTATCACACGCTCTACACCCGCATCTCGAACGG
GGACTATCCACCGAACCAGAAACTGCCGCCCGAGATGCAGCTGGCCGAGGAGCTGGGCGTCTCGCGCCCGGTGCTGCGGG
CGGCGCTGGAACGGCTGCGCGACGAGGGGATCGTCCATTCCCGGCAGGGCGCGGGCAACTACGTCCGCCCGATCCAGATC
GCGCCGCTGGGCTTTGCGCGGGTCGAGACCTTGGCCGACATCCAGCGCTGCTACGAGTTCCGCATCACCATCGAGACCGA
CGCCACATGGCTCGCAGCGCAGCGCCGCAACCCGGCCATCCTCGCCGAGATCGAGGCCGCGCTCGAACAGCTGCGGGCGG
CGACCGGGTCGATGCAGCACCGCGAGGATGCCGATTTCGCCTTCCATGTGGGCATCGCACGAGGGGCGAACAACCAGTAT
TACGAGGCCACGCTGCGCGCGCTGCGCAACCACATCAATGTGGGGATGAAGCTGCACGGCGACGCGCTGATGACCGACGG
CCAGAAGGGTCTCGAAGGGGTGCTGCGCGAACATGCGGGCATCTTCGAGGCCGTGCGGGACGGCCGGGCCGACGAGGCGC
GCGACCAGATGCGCGCCCATCTCGAACATTCCCGCGACCGCCTGTTCGGCGGGAGCCTCCTCGATCTCCGGATGCGCTGA

Upstream 100 bases:

>100_bases
CCAAGTCGGGCGGCTTCGGACCGCCGGACACGGTTGTAAAACTTGTAAAATTCCTGGAGAGGGTCTAAGCCTTGAGGGGC
GACCCCGGACGAAGCGAGCC

Downstream 100 bases:

>100_bases
GCGCGGCAGCGCTCCGGGGGGCGCGTCCCCCGGAGCCGCCCCCCCGTCAGCCGCGGCCCGCGACCAGCTTGTCCGCGTAG
GCCAGCGCCGCCAGCATGTT

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MAHLKQSTRMPLADKVYHTLYTRISNGDYPPNQKLPPEMQLAEELGVSRPVLRAALERLRDEGIVHSRQGAGNYVRPIQI
APLGFARVETLADIQRCYEFRITIETDATWLAAQRRNPAILAEIEAALEQLRAATGSMQHREDADFAFHVGIARGANNQY
YEATLRALRNHINVGMKLHGDALMTDGQKGLEGVLREHAGIFEAVRDGRADEARDQMRAHLEHSRDRLFGGSLLDLRMR

Sequences:

>Translated_239_residues
MAHLKQSTRMPLADKVYHTLYTRISNGDYPPNQKLPPEMQLAEELGVSRPVLRAALERLRDEGIVHSRQGAGNYVRPIQI
APLGFARVETLADIQRCYEFRITIETDATWLAAQRRNPAILAEIEAALEQLRAATGSMQHREDADFAFHVGIARGANNQY
YEATLRALRNHINVGMKLHGDALMTDGQKGLEGVLREHAGIFEAVRDGRADEARDQMRAHLEHSRDRLFGGSLLDLRMR
>Mature_238_residues
AHLKQSTRMPLADKVYHTLYTRISNGDYPPNQKLPPEMQLAEELGVSRPVLRAALERLRDEGIVHSRQGAGNYVRPIQIA
PLGFARVETLADIQRCYEFRITIETDATWLAAQRRNPAILAEIEAALEQLRAATGSMQHREDADFAFHVGIARGANNQYY
EATLRALRNHINVGMKLHGDALMTDGQKGLEGVLREHAGIFEAVRDGRADEARDQMRAHLEHSRDRLFGGSLLDLRMR

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790032, Length=223, Percent_Identity=30.0448430493274, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1786303, Length=229, Percent_Identity=29.2576419213974, Blast_Score=74, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 26943; Mature: 26812

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHLKQSTRMPLADKVYHTLYTRISNGDYPPNQKLPPEMQLAEELGVSRPVLRAALERLR
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
DEGIVHSRQGAGNYVRPIQIAPLGFARVETLADIQRCYEFRITIETDATWLAAQRRNPAI
HCCCEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHEEEEEECCCCEEHHHCCCCHH
LAEIEAALEQLRAATGSMQHREDADFAFHVGIARGANNQYYEATLRALRNHINVGMKLHG
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCEEECC
DALMTDGQKGLEGVLREHAGIFEAVRDGRADEARDQMRAHLEHSRDRLFGGSLLDLRMR
CHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECC
>Mature Secondary Structure 
AHLKQSTRMPLADKVYHTLYTRISNGDYPPNQKLPPEMQLAEELGVSRPVLRAALERLR
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
DEGIVHSRQGAGNYVRPIQIAPLGFARVETLADIQRCYEFRITIETDATWLAAQRRNPAI
HCCCEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHEEEEEECCCCEEHHHCCCCHH
LAEIEAALEQLRAATGSMQHREDADFAFHVGIARGANNQYYEATLRALRNHINVGMKLHG
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCEEECC
DALMTDGQKGLEGVLREHAGIFEAVRDGRADEARDQMRAHLEHSRDRLFGGSLLDLRMR
CHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]