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The map label for this gene is dmlA [H]

Identifier: 77465391

GI number: 77465391

Start: 453740

End: 454813

Strand: Direct

Name: dmlA [H]

Synonym: RSP_3389

Alternate gene names: 77465391

Gene position: 453740-454813 (Clockwise)

Preceding gene: 77465390

Following gene: 77465402

Centisome position: 48.12

GC content: 68.53

Gene sequence:

>1074_bases
ATGGCAGACAATCACCTGAAGATCGCGGTCATCGCCGGCGACGGCATCGGCAAGGAAGTGGTGCCGGAAGGGCTGCGGGT
GCTCGAAGCGGTGGCCGGGCGCCACCGGATCGACCTGCGCTTCGAGGAGTTCGACTTCGCCTCCTGCGACTACTACGCGC
GGCACGGCCAGATGATGCCCGAGGACTGGCAGGAGCGTATCGGAGGGCACGACGCCCTGTTCTTCGGCGCGGTGGGCTGG
CCTGCGACGGTGCCCGATCACGTCTCGCTCTGGGGCTCGCTGATCCAGTTCCGCCGGCAGTTCGACCAGTATGTGAACCT
GCGCCCGGCCCGGCTGATGCCGGGCGTGCCCTCGCCGCTGGCCGGGCGCGGGCCGGGCGACATCGACATGTGGATCGTGC
GCGAGAATACGGAGGGCGAATATTCCGCGATCGGCGGCAGGATGTATCCCGGCACCGAGCGCGAGATCGTGCTGCAGGAG
ACGGTGATGAGCCGTCACGGCATCGACCGTGTGCTGCGCTATGCCTTCGAGCTGGCGAACCGGCGCGAGCGGCGCAGGCT
GACCTCGGCCACCAAGTCGAACGGAATCTCGATCACCATGCCCTACTGGGACGAGCGGGTGGAGGCGATGGCGCAGGACT
ACCCGGATGTCGCCTGGGACAAGTATCACATCGACATTCTGGCCGCGCATTTCGTGCTGAACCCGGACCGGTTCGACGTG
GTCGTGGCCTCGAACCTCTTCGGCGACATCCTGTCCGACCTCGGGCCCGCCTGCACCGGCACCATCGGCATCGCGCCCTC
GGGCAACATCAACCCCGAGCGCGCCTTTCCCTCGCTGTTCGAGCCGGTCCACGGCTCGGCGCCCGACATCGCGGGACAGG
GCATCGCCAATCCGGTGGGGCAGATCTGGGCCGGGGCGATGATGCTTGACCACCTCGGCCATGGCGAGGCCGCGGCCGAG
ATCATGGCGGCCATCGAGCGCGTCCTCGCCGATCCGCGGTTGCGGACGCGGGACCTCGCCGGCGCGGCCGACACCGTCAC
CTGCGGCAAGGCCATCGTCGAGGCGCTGGGTTGA

Upstream 100 bases:

>100_bases
GATCTCCATACGCCCGACCTCGGCGGTCGGGCCACCACGCGGCAGGTGACGGATGCCCTGATCGCGGCCCTGCGCGGCCG
CAACGCCTGAGGAGCACGAC

Downstream 100 bases:

>100_bases
GGCGGTGCGGGCGGCAGGTCTTCGGGACGGGATCGATCCGCGCTCGCGGACGGGTCACGCCCTGCCGGTTCTGAAGTGAA
AGTCTTTTTTCGCCGGGCAT

Product: isocitrate/isopropylmalate dehydrogenase / tartrate dehydrogenase

Products: NA

Alternate protein names: D-malate degradation protein A; D-malate oxidase [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MADNHLKIAVIAGDGIGKEVVPEGLRVLEAVAGRHRIDLRFEEFDFASCDYYARHGQMMPEDWQERIGGHDALFFGAVGW
PATVPDHVSLWGSLIQFRRQFDQYVNLRPARLMPGVPSPLAGRGPGDIDMWIVRENTEGEYSAIGGRMYPGTEREIVLQE
TVMSRHGIDRVLRYAFELANRRERRRLTSATKSNGISITMPYWDERVEAMAQDYPDVAWDKYHIDILAAHFVLNPDRFDV
VVASNLFGDILSDLGPACTGTIGIAPSGNINPERAFPSLFEPVHGSAPDIAGQGIANPVGQIWAGAMMLDHLGHGEAAAE
IMAAIERVLADPRLRTRDLAGAADTVTCGKAIVEALG

Sequences:

>Translated_357_residues
MADNHLKIAVIAGDGIGKEVVPEGLRVLEAVAGRHRIDLRFEEFDFASCDYYARHGQMMPEDWQERIGGHDALFFGAVGW
PATVPDHVSLWGSLIQFRRQFDQYVNLRPARLMPGVPSPLAGRGPGDIDMWIVRENTEGEYSAIGGRMYPGTEREIVLQE
TVMSRHGIDRVLRYAFELANRRERRRLTSATKSNGISITMPYWDERVEAMAQDYPDVAWDKYHIDILAAHFVLNPDRFDV
VVASNLFGDILSDLGPACTGTIGIAPSGNINPERAFPSLFEPVHGSAPDIAGQGIANPVGQIWAGAMMLDHLGHGEAAAE
IMAAIERVLADPRLRTRDLAGAADTVTCGKAIVEALG
>Mature_356_residues
ADNHLKIAVIAGDGIGKEVVPEGLRVLEAVAGRHRIDLRFEEFDFASCDYYARHGQMMPEDWQERIGGHDALFFGAVGWP
ATVPDHVSLWGSLIQFRRQFDQYVNLRPARLMPGVPSPLAGRGPGDIDMWIVRENTEGEYSAIGGRMYPGTEREIVLQET
VMSRHGIDRVLRYAFELANRRERRRLTSATKSNGISITMPYWDERVEAMAQDYPDVAWDKYHIDILAAHFVLNPDRFDVV
VASNLFGDILSDLGPACTGTIGIAPSGNINPERAFPSLFEPVHGSAPDIAGQGIANPVGQIWAGAMMLDHLGHGEAAAEI
MAAIERVLADPRLRTRDLAGAADTVTCGKAIVEALG

Specific function: Catalyzes the NAD(+)-dependent oxidative decarboxylation of D-malate into pyruvate. Is essential for aerobic growth on D- malate as the sole carbon source. But is not required for anaerobic D-malate utilization, although DmlA is expressed and active in th

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=337, Percent_Identity=31.1572700296736, Blast_Score=167, Evalue=9e-42,
Organism=Homo sapiens, GI28178821, Length=348, Percent_Identity=24.7126436781609, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI28178816, Length=351, Percent_Identity=24.7863247863248, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI4758582, Length=346, Percent_Identity=27.4566473988439, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI28178838, Length=326, Percent_Identity=27.9141104294479, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI28178819, Length=204, Percent_Identity=27.4509803921569, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1788101, Length=356, Percent_Identity=66.5730337078652, Blast_Score=501, Evalue=1e-143,
Organism=Escherichia coli, GI87081683, Length=357, Percent_Identity=39.4957983193277, Blast_Score=194, Evalue=5e-51,
Organism=Escherichia coli, GI1787381, Length=398, Percent_Identity=28.643216080402, Blast_Score=115, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71986051, Length=343, Percent_Identity=32.6530612244898, Blast_Score=157, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI17550882, Length=356, Percent_Identity=28.9325842696629, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI25144293, Length=350, Percent_Identity=27.7142857142857, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17505779, Length=266, Percent_Identity=30.4511278195489, Blast_Score=124, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6322097, Length=365, Percent_Identity=34.5205479452055, Blast_Score=186, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6324709, Length=353, Percent_Identity=32.2946175637394, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6324291, Length=355, Percent_Identity=29.5774647887324, Blast_Score=149, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6319830, Length=364, Percent_Identity=28.8461538461538, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24661184, Length=355, Percent_Identity=30.9859154929577, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24643268, Length=338, Percent_Identity=31.0650887573964, Blast_Score=161, Evalue=8e-40,
Organism=Drosophila melanogaster, GI24643270, Length=338, Percent_Identity=31.0650887573964, Blast_Score=161, Evalue=8e-40,
Organism=Drosophila melanogaster, GI161078633, Length=263, Percent_Identity=31.9391634980989, Blast_Score=137, Evalue=8e-33,
Organism=Drosophila melanogaster, GI24650122, Length=263, Percent_Identity=31.9391634980989, Blast_Score=137, Evalue=8e-33,
Organism=Drosophila melanogaster, GI161078639, Length=263, Percent_Identity=31.9391634980989, Blast_Score=137, Evalue=9e-33,
Organism=Drosophila melanogaster, GI161078635, Length=263, Percent_Identity=31.9391634980989, Blast_Score=137, Evalue=9e-33,
Organism=Drosophila melanogaster, GI161078637, Length=264, Percent_Identity=31.8181818181818, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI281362242, Length=356, Percent_Identity=27.247191011236, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24648872, Length=356, Percent_Identity=27.247191011236, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI20130355, Length=356, Percent_Identity=27.247191011236, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011829 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.83 [H]

Molecular weight: Translated: 39212; Mature: 39081

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADNHLKIAVIAGDGIGKEVVPEGLRVLEAVAGRHRIDLRFEEFDFASCDYYARHGQMMP
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHCCCCCC
EDWQERIGGHDALFFGAVGWPATVPDHVSLWGSLIQFRRQFDQYVNLRPARLMPGVPSPL
HHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCC
AGRGPGDIDMWIVRENTEGEYSAIGGRMYPGTEREIVLQETVMSRHGIDRVLRYAFELAN
CCCCCCCEEEEEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
RRERRRLTSATKSNGISITMPYWDERVEAMAQDYPDVAWDKYHIDILAAHFVLNPDRFDV
HHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCCCCHHHHEEEEEHHEECCCCEEE
VVASNLFGDILSDLGPACTGTIGIAPSGNINPERAFPSLFEPVHGSAPDIAGQGIANPVG
EEHHHHHHHHHHHCCCHHCCCEECCCCCCCCHHHCCHHHHHCCCCCCCCCCCCCCCCHHH
QIWAGAMMLDHLGHGEAAAEIMAAIERVLADPRLRTRDLAGAADTVTCGKAIVEALG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHC
>Mature Secondary Structure 
ADNHLKIAVIAGDGIGKEVVPEGLRVLEAVAGRHRIDLRFEEFDFASCDYYARHGQMMP
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHCCCCCC
EDWQERIGGHDALFFGAVGWPATVPDHVSLWGSLIQFRRQFDQYVNLRPARLMPGVPSPL
HHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCC
AGRGPGDIDMWIVRENTEGEYSAIGGRMYPGTEREIVLQETVMSRHGIDRVLRYAFELAN
CCCCCCCEEEEEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
RRERRRLTSATKSNGISITMPYWDERVEAMAQDYPDVAWDKYHIDILAAHFVLNPDRFDV
HHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCCCCHHHHEEEEEHHEECCCCEEE
VVASNLFGDILSDLGPACTGTIGIAPSGNINPERAFPSLFEPVHGSAPDIAGQGIANPVG
EEHHHHHHHHHHHCCCHHCCCEECCCCCCCCHHHCCHHHHHCCCCCCCCCCCCCCCCHHH
QIWAGAMMLDHLGHGEAAAEIMAAIERVLADPRLRTRDLAGAADTVTCGKAIVEALG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]