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The map label for this gene is rnfD [H]

Identifier: 77465209

GI number: 77465209

Start: 248946

End: 250034

Strand: Direct

Name: rnfD [H]

Synonym: RSP_3195

Alternate gene names: 77465209

Gene position: 248946-250034 (Clockwise)

Preceding gene: 77465208

Following gene: 77465210

Centisome position: 26.4

GC content: 68.87

Gene sequence:

>1089_bases
ATGACCGCCCCGCTCATCATCTCGGGTCCCCATACCCACACGCTCTTCACCGTCTCGCGCACCATGCTGACGGTGAGCGC
GGCGCTGGCGCCCGCCACGCTCTTCGGCCTGTGGATGTTCGGCTGGCCCGCGATCTTCCTCTTCCTCGTGACGGTGGCCT
CGGCCTGGCTGTTCGAGGTGGCCTGCCTCCGGGTCGCGGGCAAGCCCGTGGCGCGGTTCGCGGGCGACGGCTCGGCCATC
TTGACGGGCTGGCTCGTGGCCATGACGCTGCCGCCCTATGCGCCTTGGTGGCTCGGCATGACGGGCTCGGCCATCGCCAT
CGTCATCGCGAAACATCTCTTCGGCGGCCTCGGCCAGAACCTCTTCAACCCGGCCATGGTCGCCCGCGCCATGCTGCTCA
TCGCGCTGCCCGTCCACATGACGACCTGGGTCGCGCCGGTGGGATTGCCCGAGGGGCCGGGCTTCCTCGACGGCCTCGCC
ATCACCTTCGCGGGTTCGCAGGAGTTCGACGCGGTCTCGAGCGCGTCCACGCTGAGCCACATCAAGAGCCAGATCGGCGC
GGGCGCCACCATGTCCGAGATCGCGCCGGATCTGTCCGATCTCGAGGCGCGGCTGATCGGCCTCGTTCCGGGCAGCCTCG
GCGAGACCAGCACGATCCTCCTTCTCCTCGGAGGCATCGCGTTGATGGCGCTGCGCATCATCACGCCGGTGATCCCACTC
TCGGTGCTGGGCACGCTGGCCGGGCTCTCGGCGGTGGCCTCGACCCTCGCCCCCGACCGGTTCGCGCCGCCGCTCCTGCA
TCTGACCTCGGGCTCGACGATGCTCTGCGCCTTCTTCATCGCGACCGACTATGTGACCTCGCCGGTCACTGCGATGGGCA
AGCTCGTCTATGGCGTGGCGATCGGCGCGCTCGTCTTCGTGATCCGCACCTGGGGATCGTTCCCTGAGGGCGTGGCCTTC
GCGGTGCTTTTGATGAATGCCTGCACGCCGCTGATCGAAACCTATGTGCGCCCGCGCGTCTTCGGCCGCACCCGGACGGG
CAAGTCCCTGCCGGTCAAGGCCCGGACGCCGCGCGGAGGGAAGAGATGA

Upstream 100 bases:

>100_bases
AACGCAAGGCCGAGATGGAGGCCAGGAAGCAGCGCGAGGCCGCCGCGCCCCCGCCGCCACCGCTCGCTCAGGCGATGGCC
CAGCCCGGAGGTGCCGCGCA

Downstream 100 bases:

>100_bases
CCGACGACACCGCCGCTCCGCCGCCCCGGGGCCCCGCCCGCGACTGGAAGAGCTCGCCCCTCGTCTCGGGGCTGCTCCTC
GGCCTCTTCTCGCTCGTCTC

Product: RnfD protein

Products: NA

Alternate protein names: Nitrogen fixation protein rnfD [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MTAPLIISGPHTHTLFTVSRTMLTVSAALAPATLFGLWMFGWPAIFLFLVTVASAWLFEVACLRVAGKPVARFAGDGSAI
LTGWLVAMTLPPYAPWWLGMTGSAIAIVIAKHLFGGLGQNLFNPAMVARAMLLIALPVHMTTWVAPVGLPEGPGFLDGLA
ITFAGSQEFDAVSSASTLSHIKSQIGAGATMSEIAPDLSDLEARLIGLVPGSLGETSTILLLLGGIALMALRIITPVIPL
SVLGTLAGLSAVASTLAPDRFAPPLLHLTSGSTMLCAFFIATDYVTSPVTAMGKLVYGVAIGALVFVIRTWGSFPEGVAF
AVLLMNACTPLIETYVRPRVFGRTRTGKSLPVKARTPRGGKR

Sequences:

>Translated_362_residues
MTAPLIISGPHTHTLFTVSRTMLTVSAALAPATLFGLWMFGWPAIFLFLVTVASAWLFEVACLRVAGKPVARFAGDGSAI
LTGWLVAMTLPPYAPWWLGMTGSAIAIVIAKHLFGGLGQNLFNPAMVARAMLLIALPVHMTTWVAPVGLPEGPGFLDGLA
ITFAGSQEFDAVSSASTLSHIKSQIGAGATMSEIAPDLSDLEARLIGLVPGSLGETSTILLLLGGIALMALRIITPVIPL
SVLGTLAGLSAVASTLAPDRFAPPLLHLTSGSTMLCAFFIATDYVTSPVTAMGKLVYGVAIGALVFVIRTWGSFPEGVAF
AVLLMNACTPLIETYVRPRVFGRTRTGKSLPVKARTPRGGKR
>Mature_361_residues
TAPLIISGPHTHTLFTVSRTMLTVSAALAPATLFGLWMFGWPAIFLFLVTVASAWLFEVACLRVAGKPVARFAGDGSAIL
TGWLVAMTLPPYAPWWLGMTGSAIAIVIAKHLFGGLGQNLFNPAMVARAMLLIALPVHMTTWVAPVGLPEGPGFLDGLAI
TFAGSQEFDAVSSASTLSHIKSQIGAGATMSEIAPDLSDLEARLIGLVPGSLGETSTILLLLGGIALMALRIITPVIPLS
VLGTLAGLSAVASTLAPDRFAPPLLHLTSGSTMLCAFFIATDYVTSPVTAMGKLVYGVAIGALVFVIRTWGSFPEGVAFA
VLLMNACTPLIETYVRPRVFGRTRTGKSLPVKARTPRGGKR

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase [H]

COG id: COG4658

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfD

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrB/rnfD family [H]

Homologues:

Organism=Escherichia coli, GI1787917, Length=354, Percent_Identity=40.3954802259887, Blast_Score=239, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011303
- InterPro:   IPR004338 [H]

Pfam domain/function: PF03116 NQR2_RnfD_RnfE [H]

EC number: NA

Molecular weight: Translated: 37867; Mature: 37736

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPLIISGPHTHTLFTVSRTMLTVSAALAPATLFGLWMFGWPAIFLFLVTVASAWLFEV
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ACLRVAGKPVARFAGDGSAILTGWLVAMTLPPYAPWWLGMTGSAIAIVIAKHLFGGLGQN
HHHHHCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHH
LFNPAMVARAMLLIALPVHMTTWVAPVGLPEGPGFLDGLAITFAGSQEFDAVSSASTLSH
HCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHH
IKSQIGAGATMSEIAPDLSDLEARLIGLVPGSLGETSTILLLLGGIALMALRIITPVIPL
HHHHHCCCCCHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SVLGTLAGLSAVASTLAPDRFAPPLLHLTSGSTMLCAFFIATDYVTSPVTAMGKLVYGVA
HHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
IGALVFVIRTWGSFPEGVAFAVLLMNACTPLIETYVRPRVFGRTRTGKSLPVKARTPRGG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCC
KR
CC
>Mature Secondary Structure 
TAPLIISGPHTHTLFTVSRTMLTVSAALAPATLFGLWMFGWPAIFLFLVTVASAWLFEV
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ACLRVAGKPVARFAGDGSAILTGWLVAMTLPPYAPWWLGMTGSAIAIVIAKHLFGGLGQN
HHHHHCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHH
LFNPAMVARAMLLIALPVHMTTWVAPVGLPEGPGFLDGLAITFAGSQEFDAVSSASTLSH
HCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHH
IKSQIGAGATMSEIAPDLSDLEARLIGLVPGSLGETSTILLLLGGIALMALRIITPVIPL
HHHHHCCCCCHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SVLGTLAGLSAVASTLAPDRFAPPLLHLTSGSTMLCAFFIATDYVTSPVTAMGKLVYGVA
HHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
IGALVFVIRTWGSFPEGVAFAVLLMNACTPLIETYVRPRVFGRTRTGKSLPVKARTPRGG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCC
KR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8264535; 9492268 [H]