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The map label for this gene is rnfC [H]

Identifier: 77465208

GI number: 77465208

Start: 247396

End: 248949

Strand: Direct

Name: rnfC [H]

Synonym: RSP_3194

Alternate gene names: 77465208

Gene position: 247396-248949 (Clockwise)

Preceding gene: 77465207

Following gene: 77465209

Centisome position: 26.23

GC content: 70.59

Gene sequence:

>1554_bases
ATGAGCTTTCCCCATTTCGCGGCCCTCTTCCGGCCCTCGCAGTTCTTCGGCATCCGCGGCGGCGTCCACCCCGAGACGCG
CAAGCATCTGAGCTCGGAAAGCCCCATCGAGGACATGCCGATCCCCACGCTGATCCGCGTGCCGCTCCAGCAGCATATCG
GCGCCGAGGCCGAGCCGCTGGTCAGGCGCGACGATCTGGTGGTGAAGGGCCAGCTGATCGGGCGCGCCCGCGGCCCGGTC
TCGGCCAACATACACGCGCCGACCTCGGGCCGGGTGATCGCGGTGGGCCATTTCGCGGCTCCCCATCCCTCGGGGCTGCC
CATTCCCACCATCACCATCCGCACCGACGGGCAGGACAGCTGGGGGCCCCGGCTGGGTCGCCTCCGGCCCGAGAATGCCA
CCCCCGAGGAGATCGCGGCCAAGGTGGCCGAGGCGGGGATCGTGGGCATGGGCGGCGCCACCTTCCCCTCGGCGGTGAAG
CTGAACCTGCGGGCGAAATACGACCTCCATACGCTCGTCATCAACGGGGCGGAATGCGAGCCCTACCTCACCTGCGACGA
CCGGCTGATGCGCGAGCGGGCCGAGGAGATCGCCGACGGCGTGGGGATCATGGCAAGGGCTCTGGGCGTGACGCAGATCG
TCGTGGCCATCGAGTCGAACAAGGCCGAGGCGATCTCGGCCATGCAGCGCTTCGACCGCGCGCTCGACATCCCGCTCCGG
GTGCAGGTGGTGCCGACCCAGTATCCGATGGGCTCGGAGAAGCATCTGGTGAAGATCCTGACCGGGCGCGAGACGCCGGC
GCGGGCGCTGACGGCCGATCTGGGCGTCGTCGTCCACAATGCCGCCACCGCCCATGCCGTCCATCTCGCGGTGCGCTACG
GCGAGCCTCTCCTGTCGCGCGTGGTCACGGTCTCGGGCCGGGCGGTGCGGCGGCCTGCCAATGTGCGCGTCCTGATCGGC
ACGCCGGTGTCTGAGGTCGTCGCCCATTGCGGCGGCTATGCCGAAGAGCCCGACCGGCTGCTTCTCGGCGGGCCGATGAT
GGGCCAGCCGATCCAGAACCTGCGCGTGCCGGTGGTGAAGGGCACAAGCGGCATCCTCGCCCTGACGCGCGCCGAGACCC
CCGGCGTCGATACGATGCCCTGCATCCGCTGCGGGCGCTGTGCGCAGGCCTGCCCCGTGGGCCTCACGCCCTTCGAACTC
AATGCCCGCATCCAGTCCGAGGATCTCGAGGGGGCGGGGCGGATCGGGCTTCTCGACTGCATCACCTGCGGCTGCTGCTC
CTTCACCTGCCCCTCGAACCTCCCGCTCGTGCAGACGATCCATTACGCCAAGGGCAAGCTCGCCGAGGCCGAGGGGCGCA
GGCACCAACAGGAAGAGACGAAGCGCCTCGCCGCCGCGCGGAAGGCGCGCGAGGAGGCCGCGGCCGAGGCCAAGCGGCAG
ATGATGGCGAAACGCAAGGCCGAGATGGAGGCCAGGAAGCAGCGCGAGGCCGCCGCGCCCCCGCCGCCACCGCTCGCTCA
GGCGATGGCCCAGCCCGGAGGTGCCGCGCAATGA

Upstream 100 bases:

>100_bases
CCGAGGCCATCGTGGCGCGCGTGAAGCCGAAGACCCTCAAGAGCTGGTACTGGGACAAGCCCCGCACGGCCTTCGAGGCC
CGGGGCACGGAGGTGGCGGC

Downstream 100 bases:

>100_bases
CCGCCCCGCTCATCATCTCGGGTCCCCATACCCACACGCTCTTCACCGTCTCGCGCACCATGCTGACGGTGAGCGCGGCG
CTGGCGCCCGCCACGCTCTT

Product: 4Fe-4S ferredoxin, RnfC

Products: NA

Alternate protein names: Nitrogen fixation protein rnfC [H]

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MSFPHFAALFRPSQFFGIRGGVHPETRKHLSSESPIEDMPIPTLIRVPLQQHIGAEAEPLVRRDDLVVKGQLIGRARGPV
SANIHAPTSGRVIAVGHFAAPHPSGLPIPTITIRTDGQDSWGPRLGRLRPENATPEEIAAKVAEAGIVGMGGATFPSAVK
LNLRAKYDLHTLVINGAECEPYLTCDDRLMRERAEEIADGVGIMARALGVTQIVVAIESNKAEAISAMQRFDRALDIPLR
VQVVPTQYPMGSEKHLVKILTGRETPARALTADLGVVVHNAATAHAVHLAVRYGEPLLSRVVTVSGRAVRRPANVRVLIG
TPVSEVVAHCGGYAEEPDRLLLGGPMMGQPIQNLRVPVVKGTSGILALTRAETPGVDTMPCIRCGRCAQACPVGLTPFEL
NARIQSEDLEGAGRIGLLDCITCGCCSFTCPSNLPLVQTIHYAKGKLAEAEGRRHQQEETKRLAAARKAREEAAAEAKRQ
MMAKRKAEMEARKQREAAAPPPPPLAQAMAQPGGAAQ

Sequences:

>Translated_517_residues
MSFPHFAALFRPSQFFGIRGGVHPETRKHLSSESPIEDMPIPTLIRVPLQQHIGAEAEPLVRRDDLVVKGQLIGRARGPV
SANIHAPTSGRVIAVGHFAAPHPSGLPIPTITIRTDGQDSWGPRLGRLRPENATPEEIAAKVAEAGIVGMGGATFPSAVK
LNLRAKYDLHTLVINGAECEPYLTCDDRLMRERAEEIADGVGIMARALGVTQIVVAIESNKAEAISAMQRFDRALDIPLR
VQVVPTQYPMGSEKHLVKILTGRETPARALTADLGVVVHNAATAHAVHLAVRYGEPLLSRVVTVSGRAVRRPANVRVLIG
TPVSEVVAHCGGYAEEPDRLLLGGPMMGQPIQNLRVPVVKGTSGILALTRAETPGVDTMPCIRCGRCAQACPVGLTPFEL
NARIQSEDLEGAGRIGLLDCITCGCCSFTCPSNLPLVQTIHYAKGKLAEAEGRRHQQEETKRLAAARKAREEAAAEAKRQ
MMAKRKAEMEARKQREAAAPPPPPLAQAMAQPGGAAQ
>Mature_516_residues
SFPHFAALFRPSQFFGIRGGVHPETRKHLSSESPIEDMPIPTLIRVPLQQHIGAEAEPLVRRDDLVVKGQLIGRARGPVS
ANIHAPTSGRVIAVGHFAAPHPSGLPIPTITIRTDGQDSWGPRLGRLRPENATPEEIAAKVAEAGIVGMGGATFPSAVKL
NLRAKYDLHTLVINGAECEPYLTCDDRLMRERAEEIADGVGIMARALGVTQIVVAIESNKAEAISAMQRFDRALDIPLRV
QVVPTQYPMGSEKHLVKILTGRETPARALTADLGVVVHNAATAHAVHLAVRYGEPLLSRVVTVSGRAVRRPANVRVLIGT
PVSEVVAHCGGYAEEPDRLLLGGPMMGQPIQNLRVPVVKGTSGILALTRAETPGVDTMPCIRCGRCAQACPVGLTPFELN
ARIQSEDLEGAGRIGLLDCITCGCCSFTCPSNLPLVQTIHYAKGKLAEAEGRRHQQEETKRLAAARKAREEAAAEAKRQM
MAKRKAEMEARKQREAAAPPPPPLAQAMAQPGGAAQ

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase. Stabilizes rnfB [H]

COG id: COG4656

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfC

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Probable) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787916, Length=482, Percent_Identity=39.4190871369295, Blast_Score=330, Evalue=2e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010208
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10531 SLBB [H]

EC number: NA

Molecular weight: Translated: 55380; Mature: 55249

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFPHFAALFRPSQFFGIRGGVHPETRKHLSSESPIEDMPIPTLIRVPLQQHIGAEAEPL
CCCCHHHHHHCCCHHEECCCCCCHHHHHHCCCCCCCCCCCCCHHEECCHHHHCCCCCCCC
VRRDDLVVKGQLIGRARGPVSANIHAPTSGRVIAVGHFAAPHPSGLPIPTITIRTDGQDS
CCCCCEEEEEEEEECCCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCC
WGPRLGRLRPENATPEEIAAKVAEAGIVGMGGATFPSAVKLNLRAKYDLHTLVINGAECE
CCCCCCCCCCCCCCHHHHHHHHHHCCEEECCCCCCCCEEEEEEEEEEEEEEEEEECCCCC
PYLTCDDRLMRERAEEIADGVGIMARALGVTQIVVAIESNKAEAISAMQRFDRALDIPLR
CEECCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHHHCCCCEE
VQVVPTQYPMGSEKHLVKILTGRETPARALTADLGVVVHNAATAHAVHLAVRYGEPLLSR
EEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHCEEEECCCHHEEEEEEECCCCHHHHH
VVTVSGRAVRRPANVRVLIGTPVSEVVAHCGGYAEEPDRLLLGGPMMGQPIQNLRVPVVK
HHHHCCCEEECCCCEEEEECCCHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHCCCEEEE
GTSGILALTRAETPGVDTMPCIRCGRCAQACPVGLTPFELNARIQSEDLEGAGRIGLLDC
CCCCEEEEEECCCCCCCCHHHHHHCCHHHHCCCCCCCEEECCEEECCCCCCCCCCCHHHH
ITCGCCSFTCPSNLPLVQTIHYAKGKLAEAEGRRHQQEETKRLAAARKAREEAAAEAKRQ
HHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MMAKRKAEMEARKQREAAAPPPPPLAQAMAQPGGAAQ
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
SFPHFAALFRPSQFFGIRGGVHPETRKHLSSESPIEDMPIPTLIRVPLQQHIGAEAEPL
CCCHHHHHHCCCHHEECCCCCCHHHHHHCCCCCCCCCCCCCHHEECCHHHHCCCCCCCC
VRRDDLVVKGQLIGRARGPVSANIHAPTSGRVIAVGHFAAPHPSGLPIPTITIRTDGQDS
CCCCCEEEEEEEEECCCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCC
WGPRLGRLRPENATPEEIAAKVAEAGIVGMGGATFPSAVKLNLRAKYDLHTLVINGAECE
CCCCCCCCCCCCCCHHHHHHHHHHCCEEECCCCCCCCEEEEEEEEEEEEEEEEEECCCCC
PYLTCDDRLMRERAEEIADGVGIMARALGVTQIVVAIESNKAEAISAMQRFDRALDIPLR
CEECCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHHHCCCCEE
VQVVPTQYPMGSEKHLVKILTGRETPARALTADLGVVVHNAATAHAVHLAVRYGEPLLSR
EEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHCEEEECCCHHEEEEEEECCCCHHHHH
VVTVSGRAVRRPANVRVLIGTPVSEVVAHCGGYAEEPDRLLLGGPMMGQPIQNLRVPVVK
HHHHCCCEEECCCCEEEEECCCHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHCCCEEEE
GTSGILALTRAETPGVDTMPCIRCGRCAQACPVGLTPFELNARIQSEDLEGAGRIGLLDC
CCCCEEEEEECCCCCCCCHHHHHHCCHHHHCCCCCCCEEECCEEECCCCCCCCCCCHHHH
ITCGCCSFTCPSNLPLVQTIHYAKGKLAEAEGRRHQQEETKRLAAARKAREEAAAEAKRQ
HHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MMAKRKAEMEARKQREAAAPPPPPLAQAMAQPGGAAQ
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 4Fe-4S clusters [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8264535; 9492268; 9154934 [H]