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The map label for this gene is fabI [H]

Identifier: 77465189

GI number: 77465189

Start: 228690

End: 229478

Strand: Reverse

Name: fabI [H]

Synonym: RSP_3176

Alternate gene names: 77465189

Gene position: 229478-228690 (Counterclockwise)

Preceding gene: 77465190

Following gene: 77465188

Centisome position: 24.33

GC content: 67.05

Gene sequence:

>789_bases
ATGGCCGATCTGATGAAGGGTAAACGCGGTCTGGTGATGGGGGTCGCGAACGACCGCTCGATCGCCTGGGGCATTGCACG
CGCCCTGGCCGCCGAAGGGGCCGAACTCGCCTTCTCCTATCAGGGCGAGGCGTTCGGCAAGCGGGTCGAGCCGCTGGCGG
CCTCGGTCGGCTCGGACTTCCTCGTCGATGTCGATGTGATGGACGATGCCTCGCTCGACGCCTGCTTCGGCGCGCTGAAG
GATCGCTGGGGCACGCTCGACTTCGTGGTCCATGCAATCGCCTATTCGGACAAGACCGAACTCGCCGGACGCTTCATCAA
CACCTCGCGCGAGAACTTCCGCAATTCGCTGACGATCTCGTGCTATTCCTTCATCGACATCGCGCGGCGCGCCTCGGAGC
TCATGCCCGACGGCGGCGCGCTGCTGACGCTGACCTATCAGGGGTCGAACCGGGTGACGCCCTATTACAACGTGATGGGC
GTGGCGAAATCGGCGCTGGAATCGGCGGTGCGGTATCTCGCCAATGACCTCGGGCCGCAGAAGATCCGGGTGAATGCGAT
TTCGCCCGGCCCGATGAAGACGCTCGCGGGCGCGGCCATCGCGGGCGCGCGCGCCACCTTCCGCCACACCGAGGCCAACG
CGCCCCTGCGGGCGAACGCGACGCTCGAAGCGGTGGGCGGCACGGCGGCATGGCTCGCCTCGGACTATGGCGCCTGCACC
ACGGGCGAGATCGTCCGGGTGGACGGCGGCTATCATGTGCTGGGGATGCCGCAGCCCGACCATCTCTGA

Upstream 100 bases:

>100_bases
GAGGGGGTCGAGATCGACTCGGTCCTGTCCAACAGCTTCGGCTTCGGCGGCACCAACGCCAGTCTGCTCCTGAGCAAGTT
CAACAACTGAGGGTGCGGCG

Downstream 100 bases:

>100_bases
CGACGGGGATGCGCCGGAGACGGCGCGTCCGGGATCCTGAGGAACGGGCCTGACGGCGGCCCGTTCAGTCCTGTCCCGCG
CCTCTCGCGCAGCGGCGGAG

Product: enoyl-[acyl-carrier-protein] reductase [NADH]

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 2 [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MADLMKGKRGLVMGVANDRSIAWGIARALAAEGAELAFSYQGEAFGKRVEPLAASVGSDFLVDVDVMDDASLDACFGALK
DRWGTLDFVVHAIAYSDKTELAGRFINTSRENFRNSLTISCYSFIDIARRASELMPDGGALLTLTYQGSNRVTPYYNVMG
VAKSALESAVRYLANDLGPQKIRVNAISPGPMKTLAGAAIAGARATFRHTEANAPLRANATLEAVGGTAAWLASDYGACT
TGEIVRVDGGYHVLGMPQPDHL

Sequences:

>Translated_262_residues
MADLMKGKRGLVMGVANDRSIAWGIARALAAEGAELAFSYQGEAFGKRVEPLAASVGSDFLVDVDVMDDASLDACFGALK
DRWGTLDFVVHAIAYSDKTELAGRFINTSRENFRNSLTISCYSFIDIARRASELMPDGGALLTLTYQGSNRVTPYYNVMG
VAKSALESAVRYLANDLGPQKIRVNAISPGPMKTLAGAAIAGARATFRHTEANAPLRANATLEAVGGTAAWLASDYGACT
TGEIVRVDGGYHVLGMPQPDHL
>Mature_261_residues
ADLMKGKRGLVMGVANDRSIAWGIARALAAEGAELAFSYQGEAFGKRVEPLAASVGSDFLVDVDVMDDASLDACFGALKD
RWGTLDFVVHAIAYSDKTELAGRFINTSRENFRNSLTISCYSFIDIARRASELMPDGGALLTLTYQGSNRVTPYYNVMGV
AKSALESAVRYLANDLGPQKIRVNAISPGPMKTLAGAAIAGARATFRHTEANAPLRANATLEAVGGTAAWLASDYGACTT
GEIVRVDGGYHVLGMPQPDHL

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI7705925, Length=254, Percent_Identity=27.1653543307087, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1787545, Length=256, Percent_Identity=46.484375, Blast_Score=235, Evalue=2e-63,
Organism=Escherichia coli, GI2367365, Length=247, Percent_Identity=26.7206477732794, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27814; Mature: 27683

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLMKGKRGLVMGVANDRSIAWGIARALAAEGAELAFSYQGEAFGKRVEPLAASVGSDF
CCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCHHHCCHHHHHHHHCCCCE
LVDVDVMDDASLDACFGALKDRWGTLDFVVHAIAYSDKTELAGRFINTSRENFRNSLTIS
EEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHHCCCEEEE
CYSFIDIARRASELMPDGGALLTLTYQGSNRVTPYYNVMGVAKSALESAVRYLANDLGPQ
HHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
KIRVNAISPGPMKTLAGAAIAGARATFRHTEANAPLRANATLEAVGGTAAWLASDYGACT
EEEEEEECCCCHHHHHHHHHHCCHHEEEECCCCCCEECCCEEEECCCCHHHHHCCCCCCC
TGEIVRVDGGYHVLGMPQPDHL
CCCEEEECCCEEEECCCCCCCC
>Mature Secondary Structure 
ADLMKGKRGLVMGVANDRSIAWGIARALAAEGAELAFSYQGEAFGKRVEPLAASVGSDF
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCHHHCCHHHHHHHHCCCCE
LVDVDVMDDASLDACFGALKDRWGTLDFVVHAIAYSDKTELAGRFINTSRENFRNSLTIS
EEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHHCCCEEEE
CYSFIDIARRASELMPDGGALLTLTYQGSNRVTPYYNVMGVAKSALESAVRYLANDLGPQ
HHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
KIRVNAISPGPMKTLAGAAIAGARATFRHTEANAPLRANATLEAVGGTAAWLASDYGACT
EEEEEEECCCCHHHHHHHHHHCCHHEEEECCCCCCEECCCEEEECCCCHHHHHCCCCCCC
TGEIVRVDGGYHVLGMPQPDHL
CCCEEEECCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]