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The map label for this gene is menE [H]

Identifier: 77465078

GI number: 77465078

Start: 103038

End: 104378

Strand: Direct

Name: menE [H]

Synonym: RSP_3063

Alternate gene names: 77465078

Gene position: 103038-104378 (Clockwise)

Preceding gene: 77465077

Following gene: 77465079

Centisome position: 10.93

GC content: 73.3

Gene sequence:

>1341_bases
ATGGCCGATGCGCTCGCCACGCTCTTCGCCCGGATGGCCGCAGCGGAGGGGAAGACCGCGCTGATCGAGGCCGACGGCAC
GGCGGTCTCCTATGCCGCGCTCCTTCGCCGGGTGGAGGAGGCCCTGACGCGACTGGCCTGCGAGGGTGTGCCCCGCGGCG
CCTCGGTCCAGCTCTGCGGCGATTTCGGCGCGGCCTCCGTGGTCTGGCTGCTCGCGCTCTGGCGGCACGGGGCCTGCGTC
TCGCCGGTGGCGCCCACGAGCTTCGAGCGGCGCGCGGACTTCGCCGCCGTGGCCCGGGCGAGCTGGCGGATCGAGGCCGC
GGACGAGGGGCTGGAGCGGCTCGGCGGCGGCTCGGACCATCCGCTCCTCGACCGGCTCCGCGCCGAGGGGGCGCCGGGGC
TCGTCATCTTCACCTCCGGCACGACCGGGGCGCCCAAGGGCGCCGTCCATGACATGCGCCGCCTCCTCGGCAAGTTCGCC
GCTCCCGGCAAGGATCTGGTGACGCTGGCCTTCCTGCTCTTCGACCATATCGCCGGGATCGACACGCTCCTCTATGCGCT
GTCGAATGGCTCGACGCTCGTCTGCCTGCCCGACCGGTCCGTGCCCACGGTGATGGACCGCATCCGCCGCCATCGGGTCG
AGGTGCTGCCCACCGCGCCCTCGTTCCTCAATCTCCTGCTCCTGAACGCGGGCGAGGGCCTGTCGCTGCCCAGCCTGCGC
ATCGTGACCTACGGCGCCGAGACCATGCCGCAGGCGCTGCTCGAGCGGTTGGCCGACGCACTGCCCGGCGTGCAGCTGAC
CCAGAAATACGGCACCTCCGAGCTCGGCGCCCTGCGCTCGCGCTCCGAGGGCGGGCGGTCGCTCTGGATCCGGCTCGAGG
GGGCAGGGACCGCCTGGCGCGTGGTCGAGGGCCGGCTCGAGATCCGCACCGCAACCGCGATGCTGGGCTATCTGAACGCG
CCCGATCCCTTCACCGCGGACGGCTGGTATCGAACCGGCGATCTGGTCGAAGTGGAGGGCGACCGGCTGCGGTTCCTCGG
CCGGGCGGGCGACACGATCAACGTGGGCGGCCAGAAGGTGCTTCCCGCCGAGGTCGAGGGCGCGCTTCTGGCGCTGCCGG
GCGTGGCCGAGGCGGCCGTCCATGGCGCGCCGCATCCGATCCTCGGCGCGGTCGTCGTGGCCCGGGTGCGGATGGCCGAA
GCCGGGCTCGCCCCGGCCGAGCAGCGCACGGCCCTGCGGCGCGGGCTCGCCGGGCGGCTCGAACCCTACAAGATCCCCCA
GAAGATCGAGATCGTGGCCGAGGCGCTGACGACCGAGCGGTTCAAGCAGAAGCGGGGCTGA

Upstream 100 bases:

>100_bases
TACGGCAAGATGGAGGATGTGCTCAACGTCTGCGACTTCTTCCTGCGCGACGAATCCGATTTCGTGACCGGCCAGACGAT
CTATCTCGGAGGGGTCTGAG

Downstream 100 bases:

>100_bases
CCCCGCCACCGGACCCCGCCGCCGCCCCAGGCGCGTTTCCTGATCCTGCGGCGAAAGTCTCCCGACGGCTCCTTCCGTTG
CGGCGGACTTGTGCCGAGAC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MADALATLFARMAAAEGKTALIEADGTAVSYAALLRRVEEALTRLACEGVPRGASVQLCGDFGAASVVWLLALWRHGACV
SPVAPTSFERRADFAAVARASWRIEAADEGLERLGGGSDHPLLDRLRAEGAPGLVIFTSGTTGAPKGAVHDMRRLLGKFA
APGKDLVTLAFLLFDHIAGIDTLLYALSNGSTLVCLPDRSVPTVMDRIRRHRVEVLPTAPSFLNLLLLNAGEGLSLPSLR
IVTYGAETMPQALLERLADALPGVQLTQKYGTSELGALRSRSEGGRSLWIRLEGAGTAWRVVEGRLEIRTATAMLGYLNA
PDPFTADGWYRTGDLVEVEGDRLRFLGRAGDTINVGGQKVLPAEVEGALLALPGVAEAAVHGAPHPILGAVVVARVRMAE
AGLAPAEQRTALRRGLAGRLEPYKIPQKIEIVAEALTTERFKQKRG

Sequences:

>Translated_446_residues
MADALATLFARMAAAEGKTALIEADGTAVSYAALLRRVEEALTRLACEGVPRGASVQLCGDFGAASVVWLLALWRHGACV
SPVAPTSFERRADFAAVARASWRIEAADEGLERLGGGSDHPLLDRLRAEGAPGLVIFTSGTTGAPKGAVHDMRRLLGKFA
APGKDLVTLAFLLFDHIAGIDTLLYALSNGSTLVCLPDRSVPTVMDRIRRHRVEVLPTAPSFLNLLLLNAGEGLSLPSLR
IVTYGAETMPQALLERLADALPGVQLTQKYGTSELGALRSRSEGGRSLWIRLEGAGTAWRVVEGRLEIRTATAMLGYLNA
PDPFTADGWYRTGDLVEVEGDRLRFLGRAGDTINVGGQKVLPAEVEGALLALPGVAEAAVHGAPHPILGAVVVARVRMAE
AGLAPAEQRTALRRGLAGRLEPYKIPQKIEIVAEALTTERFKQKRG
>Mature_445_residues
ADALATLFARMAAAEGKTALIEADGTAVSYAALLRRVEEALTRLACEGVPRGASVQLCGDFGAASVVWLLALWRHGACVS
PVAPTSFERRADFAAVARASWRIEAADEGLERLGGGSDHPLLDRLRAEGAPGLVIFTSGTTGAPKGAVHDMRRLLGKFAA
PGKDLVTLAFLLFDHIAGIDTLLYALSNGSTLVCLPDRSVPTVMDRIRRHRVEVLPTAPSFLNLLLLNAGEGLSLPSLRI
VTYGAETMPQALLERLADALPGVQLTQKYGTSELGALRSRSEGGRSLWIRLEGAGTAWRVVEGRLEIRTATAMLGYLNAP
DPFTADGWYRTGDLVEVEGDRLRFLGRAGDTINVGGQKVLPAEVEGALLALPGVAEAAVHGAPHPILGAVVVARVRMAEA
GLAPAEQRTALRRGLAGRLEPYKIPQKIEIVAEALTTERFKQKRG

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=529, Percent_Identity=26.2759924385633, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI187761343, Length=529, Percent_Identity=26.2759924385633, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI115511026, Length=338, Percent_Identity=27.2189349112426, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI58082049, Length=332, Percent_Identity=27.1084337349398, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI157311624, Length=343, Percent_Identity=26.8221574344023, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI157311622, Length=343, Percent_Identity=26.8221574344023, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI156151445, Length=364, Percent_Identity=24.1758241758242, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788107, Length=328, Percent_Identity=29.5731707317073, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786810, Length=362, Percent_Identity=25.6906077348066, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17558820, Length=323, Percent_Identity=29.7213622291022, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32563687, Length=466, Percent_Identity=24.8927038626609, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71996755, Length=284, Percent_Identity=27.4647887323944, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17560308, Length=351, Percent_Identity=28.4900284900285, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17560140, Length=316, Percent_Identity=26.2658227848101, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71994690, Length=354, Percent_Identity=24.2937853107345, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71994694, Length=354, Percent_Identity=24.2937853107345, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71994703, Length=354, Percent_Identity=24.2937853107345, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25141359, Length=353, Percent_Identity=23.2294617563739, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17538037, Length=329, Percent_Identity=25.2279635258359, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17559526, Length=326, Percent_Identity=26.6871165644172, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17531443, Length=337, Percent_Identity=26.7062314540059, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71985884, Length=324, Percent_Identity=25.6172839506173, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24581924, Length=328, Percent_Identity=28.9634146341463, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19922652, Length=316, Percent_Identity=28.1645569620253, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI18859661, Length=322, Percent_Identity=28.2608695652174, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648260, Length=270, Percent_Identity=27.037037037037, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21355181, Length=300, Percent_Identity=25, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 47421; Mature: 47290

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADALATLFARMAAAEGKTALIEADGTAVSYAALLRRVEEALTRLACEGVPRGASVQLCG
CHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
DFGAASVVWLLALWRHGACVSPVAPTSFERRADFAAVARASWRIEAADEGLERLGGGSDH
CCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEHHHHHHHHHCCCCCC
PLLDRLRAEGAPGLVIFTSGTTGAPKGAVHDMRRLLGKFAAPGKDLVTLAFLLFDHIAGI
HHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
DTLLYALSNGSTLVCLPDRSVPTVMDRIRRHRVEVLPTAPSFLNLLLLNAGEGLSLPSLR
HHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHEEEECCCHHHHHHHHEECCCCCCCCCEE
IVTYGAETMPQALLERLADALPGVQLTQKYGTSELGALRSRSEGGRSLWIRLEGAGTAWR
EEEECHHHHHHHHHHHHHHHCCCCEEHHHCCCHHHHHHHHCCCCCCEEEEEEECCCCEEE
VVEGRLEIRTATAMLGYLNAPDPFTADGWYRTGDLVEVEGDRLRFLGRAGDTINVGGQKV
EEECCHHEEHHHHHHHCCCCCCCCCCCCCEECCCEEEECCCCEEEEECCCCEEECCCCEE
LPAEVEGALLALPGVAEAAVHGAPHPILGAVVVARVRMAEAGLAPAEQRTALRRGLAGRL
CCHHHCCCEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
EPYKIPQKIEIVAEALTTERFKQKRG
CCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ADALATLFARMAAAEGKTALIEADGTAVSYAALLRRVEEALTRLACEGVPRGASVQLCG
HHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
DFGAASVVWLLALWRHGACVSPVAPTSFERRADFAAVARASWRIEAADEGLERLGGGSDH
CCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEHHHHHHHHHCCCCCC
PLLDRLRAEGAPGLVIFTSGTTGAPKGAVHDMRRLLGKFAAPGKDLVTLAFLLFDHIAGI
HHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
DTLLYALSNGSTLVCLPDRSVPTVMDRIRRHRVEVLPTAPSFLNLLLLNAGEGLSLPSLR
HHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHEEEECCCHHHHHHHHEECCCCCCCCCEE
IVTYGAETMPQALLERLADALPGVQLTQKYGTSELGALRSRSEGGRSLWIRLEGAGTAWR
EEEECHHHHHHHHHHHHHHHCCCCEEHHHCCCHHHHHHHHCCCCCCEEEEEEECCCCEEE
VVEGRLEIRTATAMLGYLNAPDPFTADGWYRTGDLVEVEGDRLRFLGRAGDTINVGGQKV
EEECCHHEEHHHHHHHCCCCCCCCCCCCCEECCCEEEECCCCEEEEECCCCEEECCCCEE
LPAEVEGALLALPGVAEAAVHGAPHPILGAVVVARVRMAEAGLAPAEQRTALRRGLAGRL
CCHHHCCCEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
EPYKIPQKIEIVAEALTTERFKQKRG
CCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA