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The map label for this gene is hsaB [H]

Identifier: 77465030

GI number: 77465030

Start: 48926

End: 49444

Strand: Reverse

Name: hsaB [H]

Synonym: RSP_3017

Alternate gene names: 77465030

Gene position: 49444-48926 (Counterclockwise)

Preceding gene: 77465031

Following gene: 77465029

Centisome position: 5.24

GC content: 68.21

Gene sequence:

>519_bases
ATGACCGATACCTTCCCCGCCGCCAAGGGGTTCGATCCGCGTGCCTTCCGCGCCGCCATGGGCTGTTTCGCCACCGGCGT
CACCGTCATCACCGCCCAGGCAGACGGCCGCCGCGTGGGGGTCACCGCCAACAGCTTCACCTCGCTGTCGCTGGAGCCGC
CGCTGATCCTCTGGAGCCTCCTGAAGGCCTCGTCCAGCTACCCGGTCTTCGAGGCCGCGAGCCACTTCGCCGTCAATATC
CTCGCCCATGACCAGATCGCCCTGTCGAACCGCTTCGCCTGTCGCACGGCGGACAAGTTCGACGGGCTCGAGGTCACCGA
GGGCGCGGGCGGCTGCCTGCTTCTGCCGGACACCTGCGCCTCGATCCAATGCCTGCGCGAGACCATCCTCGACGGCGGCG
ACCACTGGATCCTGATCGGCCGCGTCAGCGCCTTCGACAGTACGGATCAGGCACCGCTGCTGTTTCACCGGGGCGCCTAT
TCCATGGCACGGCCGCATCCGCAGGTCGCAGCCTCCTGA

Upstream 100 bases:

>100_bases
CTGGAGCGGGATCCCTCCCTGTTCTGAAACACCCGCCGCCCGGCCCCGCCCCCGGGCCGGGCCGCTCTCCCCTTGCCTCA
ACCAAACCGCAGAGGTCCCG

Downstream 100 bases:

>100_bases
ACCACCACCGTCAACGACAACAGGGAGGAGACCTGAATGACGACGACGCGCATCCTTGGGCTCGCGGCACTCGCGCTGAG
CCTTGCGGCCCCGGCCCGGG

Product: nitrilotriacetate monooxygenase

Products: NAD(+); reduced flavin [C]

Alternate protein names: Flavin:NADH reductase [H]

Number of amino acids: Translated: 172; Mature: 171

Protein sequence:

>172_residues
MTDTFPAAKGFDPRAFRAAMGCFATGVTVITAQADGRRVGVTANSFTSLSLEPPLILWSLLKASSSYPVFEAASHFAVNI
LAHDQIALSNRFACRTADKFDGLEVTEGAGGCLLLPDTCASIQCLRETILDGGDHWILIGRVSAFDSTDQAPLLFHRGAY
SMARPHPQVAAS

Sequences:

>Translated_172_residues
MTDTFPAAKGFDPRAFRAAMGCFATGVTVITAQADGRRVGVTANSFTSLSLEPPLILWSLLKASSSYPVFEAASHFAVNI
LAHDQIALSNRFACRTADKFDGLEVTEGAGGCLLLPDTCASIQCLRETILDGGDHWILIGRVSAFDSTDQAPLLFHRGAY
SMARPHPQVAAS
>Mature_171_residues
TDTFPAAKGFDPRAFRAAMGCFATGVTVITAQADGRRVGVTANSFTSLSLEPPLILWSLLKASSSYPVFEAASHFAVNIL
AHDQIALSNRFACRTADKFDGLEVTEGAGGCLLLPDTCASIQCLRETILDGGDHWILIGRVSAFDSTDQAPLLFHRGAYS
MARPHPQVAAS

Specific function: Catalyzes the reduction of free flavins (FMN or FAD) by NADH. Subsequently, the reduced flavins diffuse to the HsaA oxygenase subunit [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=158, Percent_Identity=29.746835443038, Blast_Score=71, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 18318; Mature: 18187

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTFPAAKGFDPRAFRAAMGCFATGVTVITAQADGRRVGVTANSFTSLSLEPPLILWSL
CCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCEEEEECCCEEEECCCCHHHHHHH
LKASSSYPVFEAASHFAVNILAHDQIALSNRFACRTADKFDGLEVTEGAGGCLLLPDTCA
HHCCCCCCHHHCCCCEEEEEEECCEEECCCCEEECCCCCCCCEEEECCCCCEEECCCCHH
SIQCLRETILDGGDHWILIGRVSAFDSTDQAPLLFHRGAYSMARPHPQVAAS
HHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDTFPAAKGFDPRAFRAAMGCFATGVTVITAQADGRRVGVTANSFTSLSLEPPLILWSL
CCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCEEEEECCCEEEECCCCHHHHHHH
LKASSSYPVFEAASHFAVNILAHDQIALSNRFACRTADKFDGLEVTEGAGGCLLLPDTCA
HHCCCCCCHHHCCCCEEEEEEECCEEECCCCEEECCCCCCCCEEEECCCCCEEECCCCHH
SIQCLRETILDGGDHWILIGRVSAFDSTDQAPLLFHRGAYSMARPHPQVAAS
HHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA