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The map label for this gene is proA [H]

Identifier: 77465005

GI number: 77465005

Start: 20095

End: 21357

Strand: Direct

Name: proA [H]

Synonym: RSP_3824

Alternate gene names: 77465005

Gene position: 20095-21357 (Clockwise)

Preceding gene: 77465004

Following gene: 77465007

Centisome position: 2.13

GC content: 70.23

Gene sequence:

>1263_bases
ATGGACGGAACGGATGTGACGATGCTCATGCGCGAGATCGGGGTTCGCGCGCGCGCTGCCGCGGCGGAGCTCGCCTTTGC
CGAACCGTCGCGCAAGGAAGAGGCCCTGAATGCCGCGGCCGAGGCGATGCTCGCCCGCTCCGACGAGATCCTCGAAGCGA
ACGGCCGCGATCTGGCTTTCGGCGCCGAGAAGGGCCTGACCCCCGCCATGATGGACCGGCTGAAGCTCGACGCCGCGCGG
ATCGACGGCATCGTCGAGGGGCTGCGGGCGGTCGCGGGTCAGCCCGATCCGGTGGGGCAGGTGATCGCCGAATGGGACCG
ACCGAGCGGCCTGCACATCCGCCGGGTCCGCACCCCCCTGGGCGTGGTGGGCGTGATCTACGAGAGCCGGCCCAACGTCA
CGGCGGATGCGGGCGCGCTCTGCCTCAAGTCCGGCAATGCGGTCATCCTGCGCGGCGGCTCCGAGAGCTTCCATTCCTCG
GGCGCGATCCACGCCGCGCTGCAGGACGGGCTGCGGCAGGCGGGCCTGCCGGTGGATGCGATCCAGCGCGTGCCGACGCG
CGACCGCGCCGCCGTGGCCGAGATGCTGCGCATGGTCGAGCATATCGACGTGATCGTGCCGCGCGGCGGCAAGGGGCTCG
TGGGCCTCGTGCAGGCCGAGGCGCGGGTGCCGGTCTTCGCCCATCTCGAGGGCATCTGTCACGTCTATGCCGACGGCGAG
GCCGATCTCGAGAAGGCGCGTCGCGTGGTGCTGAACGCCAAGACCCGCCGCACCGGCATCTGCGGCTCGGCGGAATGCCT
GCTGATCGACCGGGCCTTCCTCGCCAAGCACGGGCCGGTGCTGATCGAGGATCTGCTGAAGGCGGGCGTCGAAGTCCGCG
CCGAGGGCGAGCTGGCCCAGGTGCCGGGCACCGTGCCCGCGCAGCCCGAGGATTTCGGGCGCGAGTTTCTCGACATGATC
ATCGCGGCCAAAGTGGTGGACGGGGTGGACGAAGCGATCGCCCATATCCGCCGCTACGGCTCGAGCCACACCGAATCGAT
CCTGACCGAGAACGACGCCACGGCCGAGCGATTCTTCCGCCGGCTCGACAGCGCGATCCTGATGCGCAACGCCTCGACGC
AGTTCGCCGATGGCGGCGAGTTCGGCATGGGGGCCGAGATCGGCATCGCCACCGGCAAGATGCATGCCCGCGGCCCGGTG
GGCGCCGAGCAGCTCACGAGCTTCAAATATCTCGTGACGGGAGACGGCACGATCCGGACCTGA

Upstream 100 bases:

>100_bases
GCTACAGCGGCCGGGCAGCCCTCATCCATCGCGACGATATGGTGATCTGAGGCCGCCGCCCCGTCCGGGCGGGCCACGGC
CTGAAGGAGAGGACAGGGAC

Downstream 100 bases:

>100_bases
GGCTCAGAACGAGAGTCTGATCTCGGTCTCGCGGATCTCGGAGGTGAGGCGGCGGCCCTGACGGGACATCTCGTCGGGGA
CCAGCGCGAATTGCACGCGC

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MDGTDVTMLMREIGVRARAAAAELAFAEPSRKEEALNAAAEAMLARSDEILEANGRDLAFGAEKGLTPAMMDRLKLDAAR
IDGIVEGLRAVAGQPDPVGQVIAEWDRPSGLHIRRVRTPLGVVGVIYESRPNVTADAGALCLKSGNAVILRGGSESFHSS
GAIHAALQDGLRQAGLPVDAIQRVPTRDRAAVAEMLRMVEHIDVIVPRGGKGLVGLVQAEARVPVFAHLEGICHVYADGE
ADLEKARRVVLNAKTRRTGICGSAECLLIDRAFLAKHGPVLIEDLLKAGVEVRAEGELAQVPGTVPAQPEDFGREFLDMI
IAAKVVDGVDEAIAHIRRYGSSHTESILTENDATAERFFRRLDSAILMRNASTQFADGGEFGMGAEIGIATGKMHARGPV
GAEQLTSFKYLVTGDGTIRT

Sequences:

>Translated_420_residues
MDGTDVTMLMREIGVRARAAAAELAFAEPSRKEEALNAAAEAMLARSDEILEANGRDLAFGAEKGLTPAMMDRLKLDAAR
IDGIVEGLRAVAGQPDPVGQVIAEWDRPSGLHIRRVRTPLGVVGVIYESRPNVTADAGALCLKSGNAVILRGGSESFHSS
GAIHAALQDGLRQAGLPVDAIQRVPTRDRAAVAEMLRMVEHIDVIVPRGGKGLVGLVQAEARVPVFAHLEGICHVYADGE
ADLEKARRVVLNAKTRRTGICGSAECLLIDRAFLAKHGPVLIEDLLKAGVEVRAEGELAQVPGTVPAQPEDFGREFLDMI
IAAKVVDGVDEAIAHIRRYGSSHTESILTENDATAERFFRRLDSAILMRNASTQFADGGEFGMGAEIGIATGKMHARGPV
GAEQLTSFKYLVTGDGTIRT
>Mature_420_residues
MDGTDVTMLMREIGVRARAAAAELAFAEPSRKEEALNAAAEAMLARSDEILEANGRDLAFGAEKGLTPAMMDRLKLDAAR
IDGIVEGLRAVAGQPDPVGQVIAEWDRPSGLHIRRVRTPLGVVGVIYESRPNVTADAGALCLKSGNAVILRGGSESFHSS
GAIHAALQDGLRQAGLPVDAIQRVPTRDRAAVAEMLRMVEHIDVIVPRGGKGLVGLVQAEARVPVFAHLEGICHVYADGE
ADLEKARRVVLNAKTRRTGICGSAECLLIDRAFLAKHGPVLIEDLLKAGVEVRAEGELAQVPGTVPAQPEDFGREFLDMI
IAAKVVDGVDEAIAHIRRYGSSHTESILTENDATAERFFRRLDSAILMRNASTQFADGGEFGMGAEIGIATGKMHARGPV
GAEQLTSFKYLVTGDGTIRT

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate [H]

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=404, Percent_Identity=38.8613861386139, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI21361368, Length=404, Percent_Identity=38.8613861386139, Blast_Score=270, Evalue=2e-72,
Organism=Escherichia coli, GI1786438, Length=416, Percent_Identity=42.5480769230769, Blast_Score=351, Evalue=5e-98,
Organism=Caenorhabditis elegans, GI17569967, Length=413, Percent_Identity=38.0145278450363, Blast_Score=273, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI17569969, Length=413, Percent_Identity=38.0145278450363, Blast_Score=273, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6324899, Length=430, Percent_Identity=38.8372093023256, Blast_Score=281, Evalue=1e-76,
Organism=Drosophila melanogaster, GI21357643, Length=404, Percent_Identity=37.3762376237624, Blast_Score=265, Evalue=4e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134 [H]

Pfam domain/function: PF00171 Aldedh [H]

EC number: =1.2.1.41 [H]

Molecular weight: Translated: 44731; Mature: 44731

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGTDVTMLMREIGVRARAAAAELAFAEPSRKEEALNAAAEAMLARSDEILEANGRDLAF
CCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCEEEE
GAEKGLTPAMMDRLKLDAARIDGIVEGLRAVAGQPDPVGQVIAEWDRPSGLHIRRVRTPL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEEECCCC
GVVGVIYESRPNVTADAGALCLKSGNAVILRGGSESFHSSGAIHAALQDGLRQAGLPVDA
CEEEEEEECCCCCCCCCCCEEEECCCEEEEECCCCCHHCCCCCHHHHHHHHHHCCCCHHH
IQRVPTRDRAAVAEMLRMVEHIDVIVPRGGKGLVGLVQAEARVPVFAHLEGICHVYADGE
HHCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCEEEEECCCCCCHHEEHHCEEEEEECCC
ADLEKARRVVLNAKTRRTGICGSAECLLIDRAFLAKHGPVLIEDLLKAGVEVRAEGELAQ
HHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHCCCCHHHHHHHHCCCEEEECCCHHC
VPGTVPAQPEDFGREFLDMIIAAKVVDGVDEAIAHIRRYGSSHTESILTENDATAERFFR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHH
RLDSAILMRNASTQFADGGEFGMGAEIGIATGKMHARGPVGAEQLTSFKYLVTGDGTIRT
HHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHCCEEEEEECCCCCCC
>Mature Secondary Structure
MDGTDVTMLMREIGVRARAAAAELAFAEPSRKEEALNAAAEAMLARSDEILEANGRDLAF
CCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCEEEE
GAEKGLTPAMMDRLKLDAARIDGIVEGLRAVAGQPDPVGQVIAEWDRPSGLHIRRVRTPL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEEECCCC
GVVGVIYESRPNVTADAGALCLKSGNAVILRGGSESFHSSGAIHAALQDGLRQAGLPVDA
CEEEEEEECCCCCCCCCCCEEEECCCEEEEECCCCCHHCCCCCHHHHHHHHHHCCCCHHH
IQRVPTRDRAAVAEMLRMVEHIDVIVPRGGKGLVGLVQAEARVPVFAHLEGICHVYADGE
HHCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCEEEEECCCCCCHHEEHHCEEEEEECCC
ADLEKARRVVLNAKTRRTGICGSAECLLIDRAFLAKHGPVLIEDLLKAGVEVRAEGELAQ
HHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHCCCCHHHHHHHHCCCEEEECCCHHC
VPGTVPAQPEDFGREFLDMIIAAKVVDGVDEAIAHIRRYGSSHTESILTENDATAERFFR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHH
RLDSAILMRNASTQFADGGEFGMGAEIGIATGKMHARGPVGAEQLTSFKYLVTGDGTIRT
HHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA