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The map label for this gene is proB1

Identifier: 77465004

GI number: 77465004

Start: 18932

End: 20044

Strand: Direct

Name: proB1

Synonym: RSP_3823

Alternate gene names: 77465004

Gene position: 18932-20044 (Clockwise)

Preceding gene: 77465003

Following gene: 77465005

Centisome position: 2.01

GC content: 71.25

Gene sequence:

>1113_bases
ATGCCGCTTCAGGCGCCGGATATCCGCAGCGCCAAGCGTCTGGTCGTGAAGATCGGCTCGGCGCTCCTCGTGGATGGCGA
GGGGCTGCGCCGCCAATGGCTCTCGGCGCTGGCGCTCGACGTGGCCGAGGCGCGGACGCGGGGCACGCAGGTGATCCTCG
TCTCCTCGGGCTCGATCGCGCTCGGGCGGCGGGTGCTGCATCTGCCGCCGGGGCCGCTCGCGCTCGAACAGAGCCAGGCG
GCGGCGGCCGTGGGCCAGATCCGGCTCGCCCGCGCCTATGAGGAGGTGCTGGCCCCGCACGGCATCACCACCGCGCAGGT
GCTGGTCACGCTCGAGGATACCGAGGACCGGCGGCGCTACCTCAATTCCCGCGCCACGATGGAGACGCTGCTCGGGCTCG
GCACCGTGCCCATCGTGAACGAGAACGACACGGTCGCCACCGACGAGATCCGCTTCGGCGACAACGACCGGCTGGCCGCG
CAGATCGCGGTGACGGTGGGGGCGGATCAGCTCGTGCTCCTGTCCGACGTGGACGGCTTCTATTCGGCCAATCCCAAGGA
AGATCCCACCGCCGAGCGGTTCGATCTGGTCGAGACGATCACGCCCGCGCTCGAGGCGATGGCGGGCGATCCGGTTTCGG
GCCTGTCCAAGGGCGGCATGAAGACCAAGATCATGGCGGCCAAGACCGCCGTCGCCGGCGGCTGCGCCATGGCGATCACC
GAAGGCTCGGCGATGCGGCCGCTCAAGGCGCTGGAACAGGGCGCGGCGCGCACCTGGTTTGTGGCGCAGGGCGACCCGCA
GGCGGCGCGCAAGCGCTGGATCAACGCGATGAAGCCGCGCGGCACGATCCGGGTGGATGCGGGTGCGGTGCAGGCGCTGC
AGGCGGGCAAGTCGCTGCTGCCCGCGGGCGTGGTGGCGGTCGAGGGCAATTTCGGGCGCGGCGATCCGGTCGAGATCGCG
GGGCCCATGGGCGAGGTGCTGGGACGCGGGCTCACCCGCTACACGGCGGTCGAGGCGGTGCAGATCGCGGGCCACCGCTC
GGCCGAGATCGTGGCCATCCTCGGCTACAGCGGCCGGGCAGCCCTCATCCATCGCGACGATATGGTGATCTGA

Upstream 100 bases:

>100_bases
TCGCGGGCACCGGCCTCATGGATGTGCTGCGCGCGCTCTGGGCCGAGATCGACGGGGCGCGCGGCGACAAGGTGGAGGAG
CACGCGCCGTGGCAACCCTG

Downstream 100 bases:

>100_bases
GGCCGCCGCCCCGTCCGGGCGGGCCACGGCCTGAAGGAGAGGACAGGGACATGGACGGAACGGATGTGACGATGCTCATG
CGCGAGATCGGGGTTCGCGC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MPLQAPDIRSAKRLVVKIGSALLVDGEGLRRQWLSALALDVAEARTRGTQVILVSSGSIALGRRVLHLPPGPLALEQSQA
AAAVGQIRLARAYEEVLAPHGITTAQVLVTLEDTEDRRRYLNSRATMETLLGLGTVPIVNENDTVATDEIRFGDNDRLAA
QIAVTVGADQLVLLSDVDGFYSANPKEDPTAERFDLVETITPALEAMAGDPVSGLSKGGMKTKIMAAKTAVAGGCAMAIT
EGSAMRPLKALEQGAARTWFVAQGDPQAARKRWINAMKPRGTIRVDAGAVQALQAGKSLLPAGVVAVEGNFGRGDPVEIA
GPMGEVLGRGLTRYTAVEAVQIAGHRSAEIVAILGYSGRAALIHRDDMVI

Sequences:

>Translated_370_residues
MPLQAPDIRSAKRLVVKIGSALLVDGEGLRRQWLSALALDVAEARTRGTQVILVSSGSIALGRRVLHLPPGPLALEQSQA
AAAVGQIRLARAYEEVLAPHGITTAQVLVTLEDTEDRRRYLNSRATMETLLGLGTVPIVNENDTVATDEIRFGDNDRLAA
QIAVTVGADQLVLLSDVDGFYSANPKEDPTAERFDLVETITPALEAMAGDPVSGLSKGGMKTKIMAAKTAVAGGCAMAIT
EGSAMRPLKALEQGAARTWFVAQGDPQAARKRWINAMKPRGTIRVDAGAVQALQAGKSLLPAGVVAVEGNFGRGDPVEIA
GPMGEVLGRGLTRYTAVEAVQIAGHRSAEIVAILGYSGRAALIHRDDMVI
>Mature_369_residues
PLQAPDIRSAKRLVVKIGSALLVDGEGLRRQWLSALALDVAEARTRGTQVILVSSGSIALGRRVLHLPPGPLALEQSQAA
AAVGQIRLARAYEEVLAPHGITTAQVLVTLEDTEDRRRYLNSRATMETLLGLGTVPIVNENDTVATDEIRFGDNDRLAAQ
IAVTVGADQLVLLSDVDGFYSANPKEDPTAERFDLVETITPALEAMAGDPVSGLSKGGMKTKIMAAKTAVAGGCAMAITE
GSAMRPLKALEQGAARTWFVAQGDPQAARKRWINAMKPRGTIRVDAGAVQALQAGKSLLPAGVVAVEGNFGRGDPVEIAG
PMGEVLGRGLTRYTAVEAVQIAGHRSAEIVAILGYSGRAALIHRDDMVI

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=270, Percent_Identity=31.4814814814815, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI62912457, Length=268, Percent_Identity=31.7164179104478, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1786437, Length=362, Percent_Identity=43.3701657458564, Blast_Score=270, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17569969, Length=258, Percent_Identity=36.046511627907, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17569967, Length=258, Percent_Identity=36.046511627907, Blast_Score=131, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6321822, Length=415, Percent_Identity=32.5301204819277, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6320506, Length=413, Percent_Identity=31.4769975786925, Blast_Score=177, Evalue=3e-45,
Organism=Drosophila melanogaster, GI21357643, Length=278, Percent_Identity=33.8129496402878, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_RHOS4 (Q3IXX8)

Other databases:

- EMBL:   CP000144
- RefSeq:   YP_354507.1
- ProteinModelPortal:   Q3IXX8
- SMR:   Q3IXX8
- STRING:   Q3IXX8
- GeneID:   3721404
- GenomeReviews:   CP000144_GR
- KEGG:   rsp:RSP_3823
- NMPDR:   fig|272943.3.peg.3488
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- PhylomeDB:   Q3IXX8
- ProtClustDB:   PRK05429
- BioCyc:   RSPH272943:RSP_3823-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 38960; Mature: 38829

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLQAPDIRSAKRLVVKIGSALLVDGEGLRRQWLSALALDVAEARTRGTQVILVSSGSIA
CCCCCCCHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCE
LGRRVLHLPPGPLALEQSQAAAAVGQIRLARAYEEVLAPHGITTAQVLVTLEDTEDRRRY
ECCEEEECCCCCCEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCHHHHHH
LNSRATMETLLGLGTVPIVNENDTVATDEIRFGDNDRLAAQIAVTVGADQLVLLSDVDGF
HHHHHHHHHHHCCCCCEEECCCCCEEECEEEECCCCCEEEEEEEEECCCCEEEEECCCCC
YSANPKEDPTAERFDLVETITPALEAMAGDPVSGLSKGGMKTKIMAAKTAVAGGCAMAIT
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEEEEHHHHHCCCEEEEEE
EGSAMRPLKALEQGAARTWFVAQGDPQAARKRWINAMKPRGTIRVDAGAVQALQAGKSLL
CCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEECHHHHHHHHHCCCCC
PAGVVAVEGNFGRGDPVEIAGPMGEVLGRGLTRYTAVEAVQIAGHRSAEIVAILGYSGRA
CCCEEEEECCCCCCCCCEECCCHHHHHHCCHHHHHHHHHHEECCCCCCEEEEEEECCCCE
ALIHRDDMVI
EEEECCCCCC
>Mature Secondary Structure 
PLQAPDIRSAKRLVVKIGSALLVDGEGLRRQWLSALALDVAEARTRGTQVILVSSGSIA
CCCCCCHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCE
LGRRVLHLPPGPLALEQSQAAAAVGQIRLARAYEEVLAPHGITTAQVLVTLEDTEDRRRY
ECCEEEECCCCCCEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCHHHHHH
LNSRATMETLLGLGTVPIVNENDTVATDEIRFGDNDRLAAQIAVTVGADQLVLLSDVDGF
HHHHHHHHHHHCCCCCEEECCCCCEEECEEEECCCCCEEEEEEEEECCCCEEEEECCCCC
YSANPKEDPTAERFDLVETITPALEAMAGDPVSGLSKGGMKTKIMAAKTAVAGGCAMAIT
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEEEEHHHHHCCCEEEEEE
EGSAMRPLKALEQGAARTWFVAQGDPQAARKRWINAMKPRGTIRVDAGAVQALQAGKSLL
CCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEECHHHHHHHHHCCCCC
PAGVVAVEGNFGRGDPVEIAGPMGEVLGRGLTRYTAVEAVQIAGHRSAEIVAILGYSGRA
CCCEEEEECCCCCCCCCEECCCHHHHHHCCHHHHHHHHHHEECCCCCCEEEEEEECCCCE
ALIHRDDMVI
EEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA